Chlorobium Chlorochromatii
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Chlorobium Chlorochromatii
''Chlorobium chlorochromatii'', originally known as ''Chlorobium aggregatum'', is a symbiotic green sulfur bacteria that performs anoxygenic photosynthesis and functions as an obligate photoautotroph using reduced sulfur species as electron donors. ''Chlorobium chlorochromatii'' can be found in stratified freshwater lakes. Topic morphology ''C. chlorochromatii'' is a Gram-negative, non-motile bacillus, that exist in short chains. They are green in color and have a ring of chlorosomes around that line the inside of their cell wall. Within these chlorosomes contain the light harvesting pigment bacteriochlorophyll a and bacteriochlorophyll c which feed electrons into Photosystem 1. Ecology Photosynthetic green sulfur bacteria such as ''Chlorobium chlorochromatii'' reside in freshwater, stratified lakes beneath the micro-aerophilic algal layer in anaerobic, light-exposed environments. They have been found worldwide, mostly in holomictic or meromictic stratified lakes. Lakes that ...
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Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationsh ...
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Lake Stratification
Lake stratification is the tendency of lakes to form separate and distinct thermal layers during warm weather. Typically stratified lakes show three distinct layers, the Epilimnion comprising the top warm layer, the thermocline (or Metalimnion): the middle layer, which may change depth throughout the day, and the colder Hypolimnion extending to the floor of the lake. Definition The thermal stratification of lakes refers to a change in the temperature at different depths in the lake, and is due to the density of water varying with temperature. Cold water is denser than warm water and the epilimnion generally consists of water that is not as dense as the water in the hypolimnion. However, the temperature of maximum density for freshwater is 4 °C. In Temperate climate, temperate regions where lake water warms up and cools through the seasons, a cyclical pattern of overturn occurs that is repeated from year to year as the cold dense water at the top of the lake sinks (see stab ...
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Pseudogenes
Pseudogenes are nonfunctional segments of DNA that resemble functional genes. Most arise as superfluous copies of functional genes, either directly by DNA duplication or indirectly by reverse transcription of an mRNA transcript. Pseudogenes are usually identified when genome sequence analysis finds gene-like sequences that lack regulatory sequences needed for transcription or translation, or whose coding sequences are obviously defective due to frameshifts or premature stop codons. Most non-bacterial genomes contain many pseudogenes, often as many as functional genes. This is not surprising, since various biological processes are expected to accidentally create pseudogenes, and there are no specialized mechanisms to remove them from genomes. Eventually pseudogenes may be deleted from their genomes by chance DNA replication or DNA repair errors, or they may accumulate so many mutational changes that they are no longer recognizable as former genes. Analysis of these degeneration ...
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Microbial Metabolism
Microbial metabolism is the means by which a microbe obtains the energy and nutrients (e.g. carbon) it needs to live and reproduce. Microbes use many different types of metabolic strategies and species can often be differentiated from each other based on metabolic characteristics. The specific metabolic properties of a microbe are the major factors in determining that microbe's ecological niche, and often allow for that microbe to be useful in industrial processes or responsible for biogeochemical cycles. Types All microbial metabolisms can be arranged according to three principles: 1. How the organism obtains carbon for synthesizing cell mass:Morris, J. et al. (2019). "Biology: How Life Works", 3rd edition, W. H. Freeman. * autotrophic – carbon is obtained from carbon dioxide () * heterotrophic – carbon is obtained from organic compounds * mixotrophic – carbon is obtained from both organic compounds and by fixing carbon dioxide 2. How the organism obtains reducing e ...
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NADPH
Nicotinamide adenine dinucleotide phosphate, abbreviated NADP or, in older notation, TPN (triphosphopyridine nucleotide), is a cofactor used in anabolic reactions, such as the Calvin cycle and lipid and nucleic acid syntheses, which require NADPH as a reducing agent ('hydrogen source'). It is used by all forms of cellular life. NADPH is the reduced form of NADP. NADP differs from NAD by the presence of an additional phosphate group on the 2' position of the ribose ring that carries the adenine moiety. This extra phosphate is added by NAD+ kinase and removed by NADP+ phosphatase. Biosynthesis NADP In general, NADP+ is synthesized before NADPH is. Such a reaction usually starts with NAD+ from either the de-novo or the salvage pathway, with NAD+ kinase adding the extra phosphate group. ADP-ribosyl cyclase allows for synthesis from nicotinamide in the salvage pathway, and NADP+ phosphatase can convert NADPH back to NADH to maintain a balance. Some forms of the NAD+ kinas ...
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NADP+
Nicotinamide adenine dinucleotide phosphate, abbreviated NADP or, in older notation, TPN (triphosphopyridine nucleotide), is a Cofactor (biochemistry), cofactor used in anabolic reactions, such as the Calvin cycle and lipid and nucleic acid syntheses, which require NADPH as a reducing agent ('hydrogen source'). It is used by all forms of cellular life. NADPH is the redox, reduced form of NADP. NADP differs from NAD+, NAD by the presence of an additional phosphate group on the 2' position of the ribose ring that carries the adenine Moiety (chemistry), moiety. This extra phosphate is added by NAD+ kinase, NAD+ kinase and removed by NADP+ phosphatase. Biosynthesis NADP In general, NADP+ is synthesized before NADPH is. Such a reaction usually starts with NAD+, NAD+ from either the de-novo or the salvage pathway, with NAD+ kinase, NAD+ kinase adding the extra phosphate group. ADP-ribosyl cyclase allows for synthesis from nicotinamide in the salvage pathway, and NADP+ phosphatase ...
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Electron Transport Chain
An electron transport chain (ETC) is a series of protein complexes and other molecules that transfer electrons from electron donors to electron acceptors via redox reactions (both reduction and oxidation occurring simultaneously) and couples this electron transfer with the transfer of protons (H+ ions) across a membrane. The electrons that transferred from NADH and FADH2 to the ETC involves 4 multi-subunit large enzymes complexes and 2 mobile electron carriers. Many of the enzymes in the electron transport chain are membrane-bound. The flow of electrons through the electron transport chain is an exergonic process. The energy from the redox reactions creates an electrochemical proton gradient that drives the synthesis of adenosine triphosphate (ATP). In aerobic respiration, the flow of electrons terminates with molecular oxygen as the final electron acceptor. In anaerobic respiration, other electron acceptors are used, such as sulfate. In an electron transport chain, the redo ...
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Microbial Consortium
A microbial consortium or microbial community, is two or more bacterial or microbial groups living symbiotically. Consortiums can be endosymbiotic or ectosymbiotic, or occasionally may be both. The protist ''Mixotricha paradoxa'', itself an endosymbiont of the ''Mastotermes darwiniensis'' termite, is always found as a consortium of at least one endosymbiotic coccus, multiple ectosymbiotic species of flagellate or ciliate bacteria, and at least one species of helical ''Treponema'' bacteria that forms the basis of ''Mixotricha'' protists' locomotion. The concept of a consortium was first introduced by Johannes Reinke in 1872, and in 1877 the term symbiosis was introduced and later expanded on. Evidence for symbiosis between microbes strongly suggests it to have been a necessary precursor of the evolution of land plants and for their transition from algal communities in the sea to land. Overview Microbes hold promising application potential to raise the efficiency of bioprocesses wh ...
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Meromictic Lake
A meromictic lake is a lake which has layers of water that do not intermix. In ordinary, holomictic lakes, at least once each year, there is a physical mixing of the surface and the deep waters. The term ''meromictic'' was coined by the Austrian Ingo Findenegg in 1935, apparently based on the older word ''holomictic''. The concepts and terminology used in describing meromictic lakes were essentially complete following some additions by G. Evelyn Hutchinson in 1937. Characteristics Most lakes are ''holomictic''; that is, at least once per year, physical mixing occurs between the surface and the deep waters. In so-called monomictic lakes, the mixing occurs once per year; in dimictic lakes, the mixing occurs twice a year (typically spring and autumn), and in polymictic lakes, the mixing occurs several times a year. In meromictic lakes, however, the layers of the lake water can remain unmixed for years, decades, or centuries. Meromictic lakes can usually be divided into th ...
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Holomictic Lake
Holomictic lakes are lakes that have a uniform temperature and density from surface to bottom at a specific time during the year, which allows the lake waters to mix in the absence of stratification. Details Holomictic lakes mix at least occasionally, in contrast to meromictic lakes. Most lakes on Earth are holomictic; meromictic lakes are rare, although they may be less rare than commonly thought. Amictic lakes are sealed off by ice and never mix. There are four types of holomictic lakes: * Polymictic (mixing many times annually) * Cold Monomictic (mixing once annually; exhibiting negative stratification) * Warm Monomictic (mixing once annually; exhibiting positive stratification) * Dimictic (mixing twice annually) * Oligomictic (mixing less than once annually) See also * Thermocline A thermocline (also known as the thermal layer or the metalimnion in lakes) is a thin but distinct layer in a large body of fluid (e.g. water, as in an ocean or lake; or air, e.g. an atmos ...
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Photosystem 1
Photosystem I (PSI, or plastocyanin–ferredoxin oxidoreductase) is one of two photosystems in the photosynthetic light reactions of algae, plants, and cyanobacteria. Photosystem I is an integral membrane protein complex that uses light energy to catalyze the transfer of electrons across the thylakoid membrane from plastocyanin to ferredoxin. Ultimately, the electrons that are transferred by Photosystem I are used to produce the moderate-energy hydrogen carrier NADPH. The photon energy absorbed by Photosystem I also produces a proton-motive force that is used to generate ATP. PSI is composed of more than 110 cofactors, significantly more than Photosystem II. History This photosystem is known as PSI because it was discovered before Photosystem II, although future experiments showed that Photosystem II is actually the first enzyme of the photosynthetic electron transport chain. Aspects of PSI were discovered in the 1950s, but the significance of these discover ...
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Chlorobiota
The green sulfur bacteria are a phylum of obligately anaerobic photoautotrophic bacteria that metabolize sulfur. Green sulfur bacteria are nonmotile (except ''Chloroherpeton thalassium'', which may glide) and capable of anoxygenic photosynthesis. They live in anaerobic aquatic environments. In contrast to plants, green sulfur bacteria mainly use sulfide ions as electron donors. They are autotrophs that utilize the reverse tricarboxylic acid cycle to perform carbon fixation. They are also mixotrophs and reduce nitrogen. Characteristics Green sulfur bacteria are gram-negative rod or spherical shaped bacteria. Some types of green sulfur bacteria have gas vacuoles that allow for movement. They are photolithoautotrophs, and use light energy and reduced sulfur compounds as the electron source. Electron donors include H2, H2S, S. The major photosynthetic pigment in these bacteria is Bacteriochlorophylls ''c'' or ''d'' in green species and ''e'' in brown species, and is located in ...
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