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Western Blotting
The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Besides detecting the proteins, this technique is also utilized to visualize, distinguish, and quantify the different proteins in a complicated protein combination. Western blot technique uses three elements to achieve its task of separating a specific protein from a complex: separation by size, transfer of protein to a solid support, and marking target protein using a primary and secondary antibody to visualize. A synthetic or animal-derived antibody (known as the primary antibody) is created that recognizes and binds to a specific target protein. The electrophoresis membrane is washed in a solution containing the primary antibody, before excess antibody is washed off. A secondary antibody is added which recognizes and binds to the primary antibody. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Western Blot Workflow
Western may refer to: Places *Western, Nebraska, a village in the US *Western, New York, a town in the US *Western Creek, Tasmania, a locality in Australia *Western Junction, Tasmania, a locality in Australia *Western world, countries that identify with shared "Western" culture Arts and entertainment Films *Western (1997 film), ''Western'' (1997 film), a French road movie directed by Manuel Poirier *Western (2017 film), ''Western'' (2017 film), a German-Austrian film Genres *Western (genre), a category of fiction and visual art centered on the American Old West **Western fiction, the Western genre as featured in literature **Western music (North America), a type of American folk music Music *Westerns (EP), ''Westerns'' (EP), an EP by Pete Yorn *WSTRN, a British hip hop group from west London Business *The Western, a closed hotel/casino in Las Vegas, United States *Western Cartridge Company, a manufacturer of ammunition *Western Publishing, a defunct publishing company Edu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Immunostaining
In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941. However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods. Techniques Immunohistochemistry Immunohistochemistry or IHC staining of tissue sections (or immunocytochemistry, which is the staining of cells), is perhaps the most commonly applied immunostaining technique. While the first cases of IHC staining used fluorescent dyes (see '' immunofluorescence''), other non-fluorescent methods using enzymes such as peroxidase (see '' immunoperoxidase staining'') and alkaline phosphatase are now used. These enzymes are capable of catalysing reactions that give a coloured product that is easily detectable by light m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Post-translational Modification
Post-translational modification (PTM) is the covalent and generally enzymatic modification of proteins following protein biosynthesis. This process occurs in the endoplasmic reticulum and the golgi apparatus. Proteins are synthesized by ribosomes translating mRNA into polypeptide chains, which may then undergo PTM to form the mature protein product. PTMs are important components in cell signaling, as for example when prohormones are converted to hormones. Post-translational modifications can occur on the amino acid side chains or at the protein's C- or N- termini. They can extend the chemical repertoire of the 20 standard amino acids by modifying an existing functional group or introducing a new one such as phosphate. Phosphorylation is a highly effective mechanism for regulating the activity of enzymes and is the most common post-translational modification. Many eukaryotic and prokaryotic proteins also have carbohydrate molecules attached to them in a process called glycosyla ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biochemistry
Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all areas of the life sciences are being uncovered and developed through biochemical methodology and research. Voet (2005), p. 3. Biochemistry focuses on understanding the chemical basis which allows biological molecules to give rise to the processes that occur within living cells and between cells,Karp (2009), p. 2. in turn relating greatly to the understanding of tissues and organs, as well as organism structure and function.Miller (2012). p. 62. Biochemistry is closely related to molecular biology, which is the study of the molecular mechanisms of biological phenomena.As ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Western Blot Results For HIV Test
Western may refer to: Places *Western, Nebraska, a village in the US *Western, New York, a town in the US *Western Creek, Tasmania, a locality in Australia *Western Junction, Tasmania, a locality in Australia *Western world, countries that identify with shared "Western" culture Arts and entertainment Films * ''Western'' (1997 film), a French road movie directed by Manuel Poirier * ''Western'' (2017 film), a German-Austrian film Genres *Western (genre), a category of fiction and visual art centered on the American Old West **Western fiction, the Western genre as featured in literature **Western music (North America), a type of American folk music Music * ''Westerns'' (EP), an EP by Pete Yorn *WSTRN, a British hip hop group from west London Business *The Western, a closed hotel/casino in Las Vegas, United States *Western Cartridge Company, a manufacturer of ammunition *Western Publishing, a defunct publishing company Educational institutions *Western Washington University i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
PubMed
PubMed is a free search engine accessing primarily the MEDLINE database of references and abstracts on life sciences and biomedical topics. The United States National Library of Medicine (NLM) at the National Institutes of Health maintain the database as part of the Entrez system of information retrieval. From 1971 to 1997, online access to the MEDLINE database had been primarily through institutional facilities, such as university libraries. PubMed, first released in January 1996, ushered in the era of private, free, home- and office-based MEDLINE searching. The PubMed system was offered free to the public starting in June 1997. Content In addition to MEDLINE, PubMed provides access to: * older references from the print version of ''Index Medicus'', back to 1951 and earlier * references to some journals before they were indexed in Index Medicus and MEDLINE, for instance ''Science'', ''BMJ'', and ''Annals of Surgery'' * very recent entries to records for an article before it ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Switzerland
). Swiss law does not designate a ''capital'' as such, but the federal parliament and government are installed in Bern, while other federal institutions, such as the federal courts, are in other cities (Bellinzona, Lausanne, Luzern, Neuchâtel, St. Gallen a.o.). , coordinates = , largest_city = Zürich , official_languages = , englishmotto = "One for all, all for one" , religion_year = 2020 , religion_ref = , religion = , demonym = , german: Schweizer/Schweizerin, french: Suisse/Suissesse, it, svizzero/svizzera or , rm, Svizzer/Svizra , government_type = Federalism, Federal assembly-independent Directorial system, directorial republic with elements of a direct democracy , leader_title1 = Federal Council (Switzerland), Federal Council , leader_name1 = , leader_title2 = , leader_name2 = Walter Thurnherr , legislature = Fe ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Basel
, french: link=no, Bâlois(e), it, Basilese , neighboring_municipalities= Allschwil (BL), Hégenheim (FR-68), Binningen (BL), Birsfelden (BL), Bottmingen (BL), Huningue (FR-68), Münchenstein (BL), Muttenz (BL), Reinach (BL), Riehen (BS), Saint-Louis (FR-68), Weil am Rhein (DE-BW) , twintowns = Shanghai, Miami Beach , website = www.bs.ch Basel ( , ), also known as Basle ( ),french: Bâle ; it, Basilea ; rm, label= Sutsilvan, Basileia; other rm, Basilea . is a city in northwestern Switzerland on the river Rhine. Basel is Switzerland's third-most-populous city (after Zürich and Geneva) with about 175,000 inhabitants. The official language of Basel is (the Swiss variety of Standard) German, but the main spoken language is the local Basel German dialect. Basel is commonly considered to be the cultural capital of Switzerland and the city is famous for its many museums, including the Kunstmuseum, which is the first collection of art accessibl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Friedrich Miescher Institute For Biomedical Research
The Friedrich Miescher Institute for Biomedical Research (FMI) is a biomedical research institute founded in 1970. Based in Basel, Switzerland, the FMI is affiliated with the University of Basel and the Novartis Institutes for BioMedical Research (NIBR). It is named after Friedrich Miescher. , the FMI has around 340 collaborators, of which 20 are research group leaders, over 80 are postdoctoral collaborators and over 80 are postgraduate students participating in the FMI International PhD Program. The FMI is directed by Dirk Schübeler. The FMI is member of EU-LIFE, an alliance of leading life sciences research centres in Europe. Scientific activities The FMI is devoted to the pursuit of fundamental biomedical research. Areas of research are neurobiology, genome regulation, and multicellular systems. Research is carried out in 20 independent but highly interactive research groups. In addition, several cutting-edge technology platforms – including microscopy & imaging, c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Stanford
Stanford University, officially Leland Stanford Junior University, is a private research university in Stanford, California. The campus occupies , among the largest in the United States, and enrolls over 17,000 students. Stanford is considered among the most prestigious universities in the world. Stanford was founded in 1885 by Leland and Jane Stanford in memory of their only child, Leland Stanford Jr., who had died of typhoid fever at age 15 the previous year. Leland Stanford was a U.S. senator and former governor of California who made his fortune as a railroad tycoon. The school admitted its first students on October 1, 1891, as a coeducational and non-denominational institution. Stanford University struggled financially after the death of Leland Stanford in 1893 and again after much of the campus was damaged by the 1906 San Francisco earthquake. Following World War II, provost of Stanford Frederick Terman inspired and supported faculty and graduates' entrepreneurialism ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
George Stark
George Stark (born 1933) is an American chemist and biochemist. His research interests include protein and enzyme function and modification, interferons and cytokines, signal transduction, and gene expression. Personal life George Stark was born in New York City in 1933. His father, Jack Stark, was a restaurant owner, and his mother, Florence Stark, was a bookkeeper. He was the youngest of three children, with two older sisters, Edna and Bernyce. Stark and his family moved to semi-rural Maryland (around the Washington, D.C. area) at the start of World War II. His father opened a restaurant named “Stark's Beef and Beans,” which Stark would often help at. The family remained there until Stark was through his third year of high school, after which they relocated back to New York City, NY in 1950. Stark would then finish his education in New York City. Over the course of his career, he relocated to various cities to conduct his research ranging from California to the United King ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Northern Blot
The northern blot, or RNA blot,Gilbert, S. F. (2000) Developmental Biology, 6th Ed. Sunderland MA, Sinauer Associates. is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.Kevil, C. G., Walsh, L., Laroux, F. S., Kalogeris, T., Grisham, M. B., Alexander, J. S. (1997) An Improved, Rapid Northern Protocol. Biochem. and Biophys. Research Comm. 238:277–279. With northern blotting it is possible to observe cellular control over structure and function by determining the particular gene expression rates during differentiation and morphogenesis, as well as in abnormal or diseased conditions. Northern blotting involves the use of electrophoresis to separate RNA samples by size, and detection with a hybridization probe complementary to part of or the entire target sequence. Strictly speaking, the term 'northern blot' refers specifically to the capillary transfer of RNA from the electrophoresis gel to the blotting m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
