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Single Particle Tracking
Single-particle tracking (SPT) is the observation of the motion of individual particles within a medium. The coordinates time series, which can be either in two dimensions (''x'', ''y'') or in three dimensions (''x'', ''y'', ''z''), is referred to as a ''trajectory''. The trajectory is typically analyzed using statistical methods to extract information about the underlying dynamics of the particle. These dynamics can reveal information about the type of transport being observed (e.g., thermal or active), the medium where the particle is moving, and interactions with other particles. In the case of random motion, trajectory analysis can be used to measure the diffusion coefficient. Applications In life sciences, single-particle tracking is broadly used to quantify the dynamics of molecules/proteins in live cells (of bacteria, yeast, mammalian cells and live ''Drosophila'' embryos). It has been extensively used to study the transcription factor dynamics in live cells. This method h ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Multifocal Plane Microscopy
Multifocal plane microscopy (MUM) or multiplane microscopy or multifocus microscopy is a form of light microscopy that allows the tracking of the 3D dynamics in live cells at high temporal and spatial resolution by simultaneously imaging different focal planes within the specimen. In this methodology, the light collected from the sample by an infinity-corrected objective lens is split into two paths. In each path the split light is focused onto a detector which is placed at a specific calibrated distance from the tube lens. In this way, each detector images a distinct plane within the sample. The first developed MUM setup was capable of imaging two distinct planes within the sample. However, the setup can be modified to image more than two planes by further splitting the light in each light path and focusing it onto detectors placed at specific calibrated distances. It has later been improved for imaging up to four distinct planes. To image a greater number of focal planes, simple ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Single-molecule Experiment
A single-molecule experiment is an experiment that investigates the properties of individual molecules. Single-molecule studies may be contrasted with measurements on an ensemble or bulk collection of molecules, where the individual behavior of molecules cannot be distinguished, and only average characteristics can be measured. Since many measurement techniques in biology, chemistry, and physics are not sensitive enough to observe single molecules, single-molecule fluorescence techniques (that have emerged since the 1990s for probing various processes on the level of individual molecules) caused a lot of excitement, since these supplied many new details on the measured processes that were not accessible in the past. Indeed, since the 1990s, many techniques for probing individual molecules have been developed. The first single-molecule experiments were patch clamp experiments performed in the 1970s, but these were limited to studying ion channels. Today, systems investigated using s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diffusion
Diffusion is the net movement of anything (for example, atoms, ions, molecules, energy) generally from a region of higher concentration to a region of lower concentration. Diffusion is driven by a gradient in Gibbs free energy or chemical potential. It is possible to diffuse "uphill" from a region of lower concentration to a region of higher concentration, like in spinodal decomposition. The concept of diffusion is widely used in many fields, including physics (particle diffusion), chemistry, biology, sociology, economics, and finance (diffusion of people, ideas, and price values). The central idea of diffusion, however, is common to all of these: a substance or collection undergoing diffusion spreads out from a point or location at which there is a higher concentration of that substance or collection. A gradient is the change in the value of a quantity, for example, concentration, pressure, or temperature with the change in another variable, usually distance. A change in c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Microrheology
Microrheology is a technique used to measure the rheological properties of a medium, such as microviscosity, via the measurement of the trajectory of a flow tracer (a micrometre-sized particle). It is a new way of doing rheology, traditionally done using a rheometer. There are two types of microrheology: ''passive microrheology'' and ''active microrheology''. Passive microrheology uses inherent thermal energy to move the tracers, whereas active microrheology uses externally applied forces, such as from a magnetic field or an optical tweezer, to do so. Microrheology can be further differentiated into 1- and 2-particle methods. Passive microrheology '' Passive microrheology '' uses the thermal energy (''kT'') to move the tracers, although recent evidence suggests that active random forces inside cells may instead move the tracers in a diffusive-like manner. The trajectories of the tracers are measured optically either by microscopy, or alternatively by light scattering techniqu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Brownian Motion
Brownian motion, or pedesis (from grc, πήδησις "leaping"), is the random motion of particles suspended in a medium (a liquid or a gas). This pattern of motion typically consists of random fluctuations in a particle's position inside a fluid sub-domain, followed by a relocation to another sub-domain. Each relocation is followed by more fluctuations within the new closed volume. This pattern describes a fluid at thermal equilibrium, defined by a given temperature. Within such a fluid, there exists no preferential direction of flow (as in transport phenomena). More specifically, the fluid's overall linear and angular momenta remain null over time. The kinetic energies of the molecular Brownian motions, together with those of molecular rotations and vibrations, sum up to the caloric component of a fluid's internal energy (the equipartition theorem). This motion is named after the botanist Robert Brown, who first described the phenomenon in 1827, while looking throu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Single-particle Trajectory
Single-particle trajectories (SPTs) consist of a collection of successive discrete points causal in time. These trajectories are acquired from images in experimental data. In the context of cell biology, the trajectories are obtained by the transient activation by a laser of small dyes attached to a moving molecule. Molecules can now by visualized based on recent super-resolution microscopy, which allow routine collections of thousands of short and long trajectories. These trajectories explore part of a cell, either on the membrane or in 3 dimensions and their paths are critically influenced by the local crowded organization and molecular interaction inside the cell, as emphasized in various cell types such as neuronal cells, astrocytes, immune cells and many others. SPTs allow observing moving molecules inside cells to collect statistics SPT allowed observing moving particles. These trajectories are used to investigate cytoplasm or membrane organization, but also the cell nucl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Applied Physics Letters
''Applied Physics Letters'' is a weekly peer-reviewed scientific journal that is published by the American Institute of Physics. Its focus is rapid publication and dissemination of new experimental and theoretical papers regarding applications of physics in all disciplines of science, engineering, and modern technology. Additionally, there is an emphasis on fundamental and new developments which lay the groundwork for fields that are rapidly evolving.Home page Applied Physics Letters. American Institute of Physics. 2016.Overview Applied Physics Letters. American Institute of Physics. 2016. The journal was established in 1962. The |
Biophysical Journal
The ''Biophysical Journal'' is a biweekly peer-reviewed scientific journal published by Cell Press on behalf of the Biophysical Society. The journal was established in 1960 and covers all aspects of biophysics. The journal occasionally publishes special issues devoted to specific topics. In addition, a supplemental "abstracts issue" is published, containing abstracts of presentations at the Biophysical Society Annual Meeting. The editor-in-chief An editor-in-chief (EIC), also known as lead editor or chief editor, is a publication's editorial leader who has final responsibility for its operations and policies. The highest-ranking editor of a publication may also be titled editor, managing ... is Jane Dyson. History The following persons are or have been editor-in-chief: References External links * Cell Press academic journals Publications established in 1960 Biophysics journals Biweekly journals English-language journals Academic journals associated with learned and ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorophore
A fluorophore (or fluorochrome, similarly to a chromophore) is a fluorescent chemical compound that can re-emit light upon light excitation. Fluorophores typically contain several combined aromatic groups, or planar or cyclic molecules with several π bonds. Fluorophores are sometimes used alone, as a tracer in fluids, as a dye for staining of certain structures, as a substrate of enzymes, or as a probe or indicator (when its fluorescence is affected by environmental aspects such as polarity or ions). More generally they are covalently bonded to a macromolecule, serving as a marker (or dye, or tag, or reporter) for affine or bioactive reagents (antibodies, peptides, nucleic acids). Fluorophores are notably used to stain tissues, cells, or materials in a variety of analytical methods, i.e., fluorescent imaging and spectroscopy. Fluorescein, via its amine-reactive isothiocyanate derivative fluorescein isothiocyanate (FITC), has been one of the most popular fluorophores. Fro ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Trajectory
A trajectory or flight path is the path that an object with mass in motion follows through space as a function of time. In classical mechanics, a trajectory is defined by Hamiltonian mechanics via canonical coordinates; hence, a complete trajectory is defined by position and momentum, simultaneously. The mass might be a projectile or a satellite. For example, it can be an orbit — the path of a planet, asteroid, or comet as it travels around a central mass. In control theory, a trajectory is a time-ordered set of states of a dynamical system (see e.g. Poincaré map). In discrete mathematics, a trajectory is a sequence (f^k(x))_ of values calculated by the iterated application of a mapping f to an element x of its source. Physics of trajectories A familiar example of a trajectory is the path of a projectile, such as a thrown ball or rock. In a significantly simplified model, the object moves only under the influence of a uniform gravitational force field. This can be ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
