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Sf21
Sf21 (officially called IPLB-Sf21-AE) is a continuous cell line developed from ovaries of the Fall Army worm, ''Spodoptera frugiperda'', a moth species that is an agricultural pest on corn and other grass species. It was originally developed in the United States at the Henry A. Wallace Beltsville Agricultural Research Center. Sf9 is a substrain ( clone) of these cells that was isolated from Sf21 by researchers at Texas A&M University. Both the clone and parent strains of the cells have been extensively used in research on viruses, especially baculovirus ''Baculoviridae'' is a family of viruses. Arthropod Arthropods (, (gen. ποδός)) are invertebrate animals with an exoskeleton, a Segmentation (biology), segmented body, and paired jointed appendages. Arthropods form the phylum Arthropod ...es in their use for producing recombinant proteins. References {{cite journal , author=Vaughn, J. L. , author2=Goodwin, R. H. , author3=Tompkins, G. J. , author4=McCawley ...
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Sf21
Sf21 (officially called IPLB-Sf21-AE) is a continuous cell line developed from ovaries of the Fall Army worm, ''Spodoptera frugiperda'', a moth species that is an agricultural pest on corn and other grass species. It was originally developed in the United States at the Henry A. Wallace Beltsville Agricultural Research Center. Sf9 is a substrain ( clone) of these cells that was isolated from Sf21 by researchers at Texas A&M University. Both the clone and parent strains of the cells have been extensively used in research on viruses, especially baculovirus ''Baculoviridae'' is a family of viruses. Arthropod Arthropods (, (gen. ποδός)) are invertebrate animals with an exoskeleton, a Segmentation (biology), segmented body, and paired jointed appendages. Arthropods form the phylum Arthropod ...es in their use for producing recombinant proteins. References {{cite journal , author=Vaughn, J. L. , author2=Goodwin, R. H. , author3=Tompkins, G. J. , author4=McCawley ...
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Sf9 (cells)
Sf9 cells, a clonal isolate of ''Spodoptera frugiperda'' Sf21 cells (IPLB-Sf21-AE), are commonly used in insect cell culture for recombinant protein production using baculovirus. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension. Sf9 Rhabdovirus It has previously been shown that some Sf9 cell lines harbor a negative sense Rhabdovirus called Spodoptera frugiperda rhabdovirus ''Spodoptera'' is a genus of moths of the family Noctuidae erected by Achille Guenée in 1852. Many are known as pest insects. The larvae are sometimes called armyworms. The roughly thirty species are distributed across six continents. Descri ... (SfRV). However, not all tested Sf9 cells appear to be infected with this virus. SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.{{cite journal, author=Maghodia AB, Jarvis DL, title=Infectivity of Sf-rhabdovirus variants in i ...
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Fall Armyworm
The fall armyworm (''Spodoptera frugiperda'') is a species in the order Lepidoptera and one of the species of the fall armyworm moths distinguished by their larval life stage. The term "armyworm" can refer to several species, often describing the large-scale invasive behavior of the species' larval stage. It is regarded as a pest and can damage and destroy a wide variety of crops, which causes large economic damage. Its scientific name derives from ''frugiperda,'' which is Latin for ''lost fruit'', named because of the species' ability to destroy crops. Because of its propensity for destruction, the fall armyworm's habits and possibilities for crop protection have been studied in depth. It is also a notable case for studying sympatric speciation, as it appears to be diverging into two species currently. Another remarkable trait of the larva is that they consistently practice cannibalism, despite its fitness costs. The fall armyworm is active at a different time of year from th ...
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Spodoptera Frugiperda
The fall armyworm (''Spodoptera frugiperda'') is a species in the order Lepidoptera and one of the species of the fall armyworm moths distinguished by their larval life stage. The term "armyworm" can refer to several species, often describing the large-scale invasive behavior of the species' larval stage. It is regarded as a pest and can damage and destroy a wide variety of crops, which causes large economic damage. Its scientific name derives from ''frugiperda,'' which is Latin for ''lost fruit'', named because of the species' ability to destroy crops. Because of its propensity for destruction, the fall armyworm's habits and possibilities for crop protection have been studied in depth. It is also a notable case for studying sympatric speciation, as it appears to be diverging into two species currently. Another remarkable trait of the larva is that they consistently practice cannibalism, despite its fitness costs. The fall armyworm is active at a different time of year from the ...
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Baculovirus
''Baculoviridae'' is a family of viruses. Arthropod Arthropods (, (gen. ποδός)) are invertebrate animals with an exoskeleton, a Segmentation (biology), segmented body, and paired jointed appendages. Arthropods form the phylum Arthropoda. They are distinguished by their jointed limbs and Arth ...s, among the most studied being Lepidoptera, Hymenoptera and Diptera, serve as natural hosts. Currently, 85 Virus classification, species are placed in this family, assigned to four genera. Baculoviruses are known to infect insects, with over 600 host species having been described. Immature (larval) forms of lepidopteran species (moths and butterflies) are the most common hosts, but these viruses have also been found infecting sawfly, sawflies, and mosquitoes. Although baculoviruses are capable of entering mammalian cells in culture, they are not known to be capable of replication in mammalian or other vertebrate animal cells. Starting in the 1940s, they were used and studied wi ...
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Cell Line
An immortalised cell line is a population of cells from a multicellular organism which would normally not proliferate indefinitely but, due to mutation, have evaded normal cellular senescence and instead can keep undergoing division. The cells can therefore be grown for prolonged periods ''in vitro''. The mutations required for immortality can occur naturally or be intentionally induced for experimental purposes. Immortal cell lines are a very important tool for research into the biochemistry and cell biology of multicellular organisms. Immortalised cell lines have also found uses in biotechnology. An immortalised cell line should not be confused with stem cells, which can also divide indefinitely, but form a normal part of the development of a multicellular organism. Relation to natural biology and pathology There are various immortal cell lines. Some of them are normal cell lines (e.g. derived from stem cells). Other immortalised cell lines are the ''in vitro'' equivalent ...
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Moth
Moths are a paraphyletic group of insects that includes all members of the order Lepidoptera that are not butterflies, with moths making up the vast majority of the order. There are thought to be approximately 160,000 species of moth, many of which have yet to be described. Most species of moth are nocturnal, but there are also crepuscular and diurnal species. Differences between butterflies and moths While the butterflies form a monophyletic group, the moths, comprising the rest of the Lepidoptera, do not. Many attempts have been made to group the superfamilies of the Lepidoptera into natural groups, most of which fail because one of the two groups is not monophyletic: Microlepidoptera and Macrolepidoptera, Heterocera and Rhopalocera, Jugatae and Frenatae, Monotrysia and Ditrysia.Scoble, MJ 1995. The Lepidoptera: Form, function and diversity. Oxford, UK: Oxford University Press; 404 p. Although the rules for distinguishing moths from butterflies are not well establishe ...
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Henry A
Henry may refer to: People *Henry (given name) *Henry (surname) * Henry Lau, Canadian singer and musician who performs under the mononym Henry Royalty * Portuguese royalty ** King-Cardinal Henry, King of Portugal ** Henry, Count of Portugal, Henry of Burgundy, Count of Portugal (father of Portugal's first king) ** Prince Henry the Navigator, Infante of Portugal ** Infante Henrique, Duke of Coimbra (born 1949), the sixth in line to Portuguese throne * King of Germany **Henry the Fowler (876–936), first king of Germany * King of Scots (in name, at least) ** Henry Stuart, Lord Darnley (1545/6–1567), consort of Mary, queen of Scots ** Henry Benedict Stuart, the 'Cardinal Duke of York', brother of Bonnie Prince Charlie, who was hailed by Jacobites as Henry IX * Four kings of Castile: **Henry I of Castile **Henry II of Castile **Henry III of Castile **Henry IV of Castile * Five kings of France, spelt ''Henri'' in Modern French since the Renaissance to italianize the name and to ...
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Cloning
Cloning is the process of producing individual organisms with identical or virtually identical DNA, either by natural or artificial means. In nature, some organisms produce clones through asexual reproduction. In the field of biotechnology, cloning is the process of creating cloned organisms (copies) of Cell (biology), cells and of DNA fragments (molecular cloning). Etymology Coined by Herbert J. Webber, the term clone derives from the Ancient Greek word (), ''twig'', which is the process whereby a new plant is created from a twig. In botany, the term ''lusus'' was used. In horticulture, the spelling ''clon'' was used until the early twentieth century; the final ''e'' came into use to indicate the vowel is a "long o" instead of a "short o". Since the term entered the popular lexicon in a more general context, the spelling ''clone'' has been used exclusively. Natural cloning Cloning is a natural form of reproduction that has allowed life forms to spread for hundreds of millio ...
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Texas A&M University
Texas A&M University (Texas A&M, A&M, or TAMU) is a public, land-grant, research university in College Station, Texas. It was founded in 1876 and became the flagship institution of the Texas A&M University System in 1948. As of late 2021, Texas A&M has the largest student body in the United States, and is the only university in Texas to hold simultaneous designations as a land, sea, and space grant institution. In 2001, it was inducted into the Association of American Universities. The university's students, alumni, and sports teams are known as Aggies, and its athletes compete in eighteen varsity sports as a member of the Southeastern Conference. The university was the first public higher-education institution in Texas; it opened for classes on October 4, 1876, as the Agricultural and Mechanical College of Texas (A.M.C.) under the provisions of the 1862 Morrill Land-Grant Act. In the following decades, the college grew in size and scope, expanding to its largest enrol ...
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Virus
A virus is a submicroscopic infectious agent that replicates only inside the living cells of an organism. Viruses infect all life forms, from animals and plants to microorganisms, including bacteria and archaea. Since Dmitri Ivanovsky's 1892 article describing a non-bacterial pathogen infecting tobacco plants and the discovery of the tobacco mosaic virus by Martinus Beijerinck in 1898,Dimmock p. 4 more than 9,000 virus species have been described in detail of the millions of types of viruses in the environment. Viruses are found in almost every ecosystem on Earth and are the most numerous type of biological entity. The study of viruses is known as virology, a subspeciality of microbiology. When infected, a host cell is often forced to rapidly produce thousands of copies of the original virus. When not inside an infected cell or in the process of infecting a cell, viruses exist in the form of independent particles, or ''virions'', consisting of (i) the genetic material, i. ...
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Insect Cell Lines
Insects (from Latin ') are pancrustacean hexapod invertebrates of the class Insecta. They are the largest group within the arthropod phylum. Insects have a chitinous exoskeleton, a three-part body (head, thorax and abdomen), three pairs of jointed legs, compound eyes and one pair of antennae. Their blood is not totally contained in vessels; some circulates in an open cavity known as the haemocoel. Insects are the most diverse group of animals; they include more than a million described species and represent more than half of all known living organisms. The total number of extant species is estimated at between six and ten million; In: potentially over 90% of the animal life forms on Earth are insects. Insects may be found in nearly all environments, although only a small number of species reside in the oceans, which are dominated by another arthropod group, crustaceans, which recent research has indicated insects are nested within. Nearly all insects hatch from eggs. Inse ...
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