Sf9 cells, a clonal isolate of ''

Spodoptera frugiperda The fall armyworm (''Spodoptera frugiperda'') is a species in the order Lepidoptera and one of the species of the fall armyworm moths distinguished by their larval life stage. The term "armyworm" can refer to several species, often describing the ...

'' Sf21 cells (IPLB-Sf21-AE), are commonly used in

insect cell culture The use of insect cell lines as production hosts is an emerging technology for the production of bio pharmaceuticals. There are currently more than 100 insect cell lines available for recombinant protein production with lines derived from ''Bombyx m ...

for recombinant protein production using

baculovirus ''Baculoviridae'' is a family of viruses. Arthropod Arthropods (, (gen. ποδός)) are invertebrate animals with an exoskeleton, a Segmentation (biology), segmented body, and paired jointed appendages. Arthropods form the phylum Arthropod ...

. They were originally established from ovarian tissue. They can be grown in the absence of serum, and can be cultured attached or in suspension.

Sf9 Rhabdovirus

It has previously been shown that some Sf9 cell lines harbor a negative sense

Rhabdovirus ''Rhabdoviridae'' is a family of negative-strand RNA viruses in the order '' Mononegavirales''. Vertebrates (including mammals and humans), invertebrates, plants, fungi and protozoans serve as natural hosts. Diseases associated with member vir ...

called

Spodoptera frugiperda rhabdovirus ''Spodoptera'' is a genus of moths of the family Noctuidae erected by Achille Guenée in 1852. Many are known as pest insects. The larvae are sometimes called armyworms. The roughly thirty species are distributed across six continents. Descri ...

(SfRV). However, not all tested Sf9 cells appear to be infected with this virus. SfRV appears to be insect-specific and does not appear to infect mammalian cell lines.{{cite journal, author=Maghodia AB, Jarvis DL, title=Infectivity of Sf-rhabdovirus variants in insect and mammalian cell lines. , journal=Virology , year= 2017 , volume= 512 , issue= , pages= 234–245 , pmid=29024851 , doi=10.1016/j.virol.2017.09.025 , pmc=5651996

References

External links

Cellosaurus entry for Sf9
Insect cell lines Spodoptera