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Rhodamine
Rhodamine is a family of related dyes, a subset of the triarylmethane dyes. They are derivatives of xanthene. Important members of the rhodamine family are Rhodamine 6G, Rhodamine 123, and Rhodamine B. They are mainly used to dye paper and inks, but they lack the lightfastness for fabric dyeing. Use Aside from their major applications, they are often used as a tracer dye, e.g. to determine the rate and direction of flow and transport of water. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 123 is used in biochemistry to inhibit mitochondrion function. Rhodamine 123 appears to bind to the mitochondrial membranes and inhibit transport processes, especially the electron transport chain, thus slowing down cellular respiration. It is a substra ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rhodamine B
Rhodamine B is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with fluorometers. Rhodamine B is used in biology as a staining fluorescent dye, sometimes in combination with auramine O, as the auramine-rhodamine stain to demonstrate acid-fast organisms, notably ''Mycobacterium''. Rhodamine dyes are also used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. It is also used in rose milk, a popular Indian beverage. Other uses Rhodamine B is often mixed with herbicides to show where they have been used. It is also being tested for use as a biomarker in oral rabies vaccines for wildlife, such as raccoons, to identify animals that have eaten a vaccine bait. The rhodamine is incorporated into the animal's whiskers and teeth. Rhodam ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rhodamine 6G
Rhodamine 6G is a highly fluorescent rhodamine family dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Forms Rhodamine 6G usually comes in three different forms. Rhodamine 6G chloride is a bronze/red powder with the chemical formula C28H31ClN2O3. Although highly soluble, this formulation is very corrosive to all metals except stainless steel. Other formulations are less soluble, but also less corrosive. Rhodamine 6G perchlorate (C28H31ClN2O7) comes in the form of red crystals, while rhodamine 6G tetrafluoroborate (C28H31BF4N2O3) appears as maroon crystals. Solubility Butanol (40 g/L), ethanol (80 g/L), methanol (400 g/L), propanol ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rhodamine Skeleton
Rhodamine is a family of related dyes, a subset of the triarylmethane dyes. They are derivatives of xanthene. Important members of the rhodamine family are Rhodamine 6G, Rhodamine 123, and Rhodamine B. They are mainly used to dye paper and inks, but they lack the lightfastness for fabric dyeing. Use Aside from their major applications, they are often used as a tracer dye, e.g. to determine the rate and direction of flow and transport of water. Rhodamine dyes fluorescence, fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as Fluorescence microscope, fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine 123 is used in biochemistry to inhibit mitochondrion function. Rhodamine 123 appears to bind to the mitochondrial membranes and inhibit transport processes, especially the electron transport chain, thus slowing down ce ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rhodamine 123
Rhodamine 123 is a chemical compound and a dye. It is often used as a tracer dye within water to determine the rate and direction of flow and transport. Rhodamine dyes fluoresce and can thus be detected easily and inexpensively with instruments called fluorometers. Rhodamine dyes are used extensively in biotechnology applications such as fluorescence microscopy, flow cytometry, fluorescence correlation spectroscopy and ELISA. Rhodamine fluorescence can also be used as a measure of membrane polarization in live cell assays both within mitochondria and with bacteria. This use relies on the fact that rhodamine 123 accumulates in membranes in a manner which is dependent on membrane polarization. The absorption of rhodamine 123 peaks around 505 nm and luminescence is tunable around 560 nm when used as a laser dye. Its luminescence quantum yield is 0.90.R. F. Kubin and A. N. Fletcher, "Fluorescence quantum yields of some rhodamine dyes.J. Luminescence 27 (1982) 455/ref> R ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dye Laser
A dye laser is a laser that uses an organic dye as the lasing medium, usually as a liquid solution. Compared to gases and most solid state lasing media, a dye can usually be used for a much wider range of wavelengths, often spanning 50 to 100 nanometers or more. The wide bandwidth makes them particularly suitable for tunable lasers and pulsed lasers. The dye rhodamine 6G, for example, can be tuned from 635 nm (orangish-red) to 560 nm (greenish-yellow), and produce pulses as short as 16 femtoseconds. Moreover, the dye can be replaced by another type in order to generate an even broader range of wavelengths with the same laser, from the near-infrared to the near-ultraviolet, although this usually requires replacing other optical components in the laser as well, such as dielectric mirrors or pump lasers. Dye lasers were independently discovered by P. P. Sorokin and F. P. Schäfer (and colleagues) in 1966. In addition to the usual liquid state, dye lasers are also availa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence Correlation Spectroscopy
Fluorescence correlation spectroscopy (FCS) is a statistical analysis, via time correlation, of stationary fluctuations of the fluorescence intensity. Its theoretical underpinning originated from L. Onsager's regression hypothesis. The analysis provides kinetic parameters of the physical processes underlying the fluctuations. One of the interesting applications of this is an analysis of the concentration fluctuations of fluorescent particles (molecules) in solution. In this application, the fluorescence emitted from a very tiny space in solution containing a small number of fluorescent particles (molecules) is observed. The fluorescence intensity is fluctuating due to Brownian motion of the particles. In other words, the number of the particles in the sub-space defined by the optical system is randomly changing around the average number. The analysis gives the average number of fluorescent particles and average diffusion time, when the particle is passing through the space. Eventua ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Triarylmethane Dye
Triarylmethane dyes are synthetic organic compounds containing triphenylmethane backbones. As dyes, these compounds are intensely colored. They are produced industrially as dyes. Families Triarylmethane dyes can be grouped into families according to the nature of the substituents on the aryl groups. In some cases, the anions associated with the cationic dyes (say crystal violet) vary even though the name of the dye does not. Often it is shown as chloride. Methyl violet dyes Methyl violet dyes have dimethylamino groups at the ''p''-positions of two aryl groups. Image:Methyl Violet 2B.png, Methyl violet 2B Image:Methyl Violet 6B.png, Methyl violet 6B Image:Methyl Violet 10B.png, Methyl violet 10B Fuchsine dyes Fuchsine dyes have primary or secondary amines (NH2 or NHMe) functional groups at the ''p''-positions of each aryl group. File:Pararosaniline.png, Pararosaniline File:Rosaniline hydrochloride.svg, Fuchsine (hydrochloride salt) Neofuchsin.svg, New fuchsine (As chlorid ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xanthene
Xanthene (9''H''-xanthene, 10''H''-9-oxaanthracene) is the organic compound with the formula CH2 6H4sub>2O. It is a yellow solid that is soluble in common organic solvents. Xanthene itself is an obscure compound, but many of its derivatives are useful dyes. Xanthene dyes Dyes that contain a xanthene core include fluorescein, eosins, and rhodamines. Xanthene dyes tend to be fluorescent, yellow to pink to bluish red, brilliant dyes. Many xanthene dyes can be prepared by condensation of derivates of phthalic anhydride with derivates of resorcinol or 3-aminophenol. Further reading * See also * Xanthone * Xanthydrol Xanthydrol is an organic chemical compound. Its formula is C13 H10 O2. Its total molecular weight is 198.221 g/ mol. Xanthydrol is used to test the levels of urea in the bloodstream. Synthesis Xanthydrol can be produced by the reduction of xan ... References {{reflist Fungicides ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sulforhodamine 101
Sulforhodamine 101 (SR101) is a red fluorescent dye. In neurophysiological experiments which comprise calcium imaging methods, it can be used for a counterstaining of astrocytes to be able to analyze data from neurons separately. However, in addition to labeling astrocytes, SR101 labels myelinating oligodendrocytes. SR101 has been reported to affect excitability of neurons and should therefore be used with caution. A sulfonyl chloride derivative of sulforhodamine 101, known as Texas Red Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohis ..., is used for conjugation to a number of functional groups, especially primary amines. References {{Reflist Benzenesulfonates Rhodamine dyes Nitrogen heterocycles Oxygen heterocycles ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Texas Red
Texas Red or sulforhodamine 101 acid chloride is a red fluorescent dye, used in histology for staining cell specimens, for sorting cells with fluorescent-activated cell sorting machines, in fluorescence microscopy applications, and in immunohistochemistry. Texas Red fluoresces at about 615 nm, and the peak of its absorption spectrum is at 589 nm. The powder is dark purple. Solutions can be excited by a dye laser tuned to 595-605 nm, or less efficiently a krypton laser at 567 nm. The absorption extinction coefficient at 596 nm is about 85,000 M−1cm−1. The compound is usually a mixture of two monosulfonyl chlorides, i.e., as pictured, or with the SO3 and SO2Cl groups exchanged. It can be used as a marker of proteins, with which it easily forms conjugates via the sulfonyl chloride (SO2Cl) group. In water, the sulfonyl chloride group of unreacted Texas Red molecules hydrolyses to sulfonate and the molecule becomes the very water-soluble sulforhodamine ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorometer
A fluorometer, fluorimeter or fluormeter is a device used to measure parameters of visible spectrum fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. These parameters are used to identify the presence and the amount of specific molecules in a medium. Modern fluorometers are capable of detecting fluorescent molecule concentrations as low as 1 part per trillion. Fluorescence analysis can be orders of magnitude more sensitive than other techniques. Applications include chemistry/biochemistry, medicine, environmental monitoring. For instance, they are used to measure chlorophyll fluorescence to investigate plant physiology. Components and Design Typically fluorometers utilize a double beam. These two beams work in tandem to decrease the noise created from radiant power fluctuations. The upper beam is passed through a filter or monochromator and passes through the sample. The lower beam is passed through an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Isothiocyanate
In organic chemistry, isothiocyanate is the functional group , formed by substituting the oxygen in the isocyanate group with a sulfur. Many natural isothiocyanates from plants are produced by enzymatic conversion of metabolites called glucosinolates. These natural isothiocyanates, such as allyl isothiocyanate, are also known as mustard oils. An artificial isothiocyanate, phenyl isothiocyanate, is used for amino acid sequencing in the Edman degradation. Cruciferous vegetables, such as bok choy, broccoli, cabbage, cauliflower, kale, and others, are rich sources of glucosinolate precursors of isothiocyanates. Although there has been some basic research on how isothiocyanates might exert biological effects ''in vivo'', there is no high-quality evidence to date for its efficacy against human diseases. Structure Typical bond angles for and linkages in aryl isothiocyanates are 165° and 177°, respectively. The and distances are 117 and 158 pms. Synthesis and reactions Isothiocy ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
