Phytophthora Kernoviae
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Phytophthora Kernoviae
''Phytophthora kernoviae'' is a plant pathogen that mainly infects European beech (''Fagus sylvatica'') and ''Rhododendron ponticum''. It was first identified in 2003 in Cornwall, UK when scientists were surveying for the presence of ''Phytophthora ramorum''. This made it the third new ''Phytophthora'' species to be found in the UK in a decade. It was named ''Phytophthora kernoviae'', after the ancient name for Cornwall, Kernow. It causes large stem lesions on beech and necrosis of stems and leaves of ''Rhododendron ponticum''. It is self-fertile. It has also been isolated from ''Quercus robur'' and '' Liriodendron tulipifera''. The original paper describing the species, stated it can infect ''Magnolia'' and ''Camellia'' species, '' Pieris formosa'', ''Gevuina avellana'', '' Michelia doltsopa'' and '' Quercus ilex''. Since then many other plants have been identified as natural hosts of the pathogen. Molecular analysis has revealed that an infection on ''Pinus radiata'', reco ...
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Clive Michael Brasier
Clive is a name. People and fictional characters with the name include: People Given name * Clive Allen (born 1961), English football player * Clive Anderson (born 1952), British television, radio presenter, comedy writer and former barrister * Clive Barker (born 1952), English writer, film director and visual artist * Clive Barker (artist, born 1940), British pop artist * Clive Barker (soccer) (born 1944), South African coach * Clive Barnes (1927–2008), English writer and critic, dance and theater critic for ''The New York Times'' * Clive Bell (1881–1964), English art critic * Clive Brook (1887–1974), British film actor * Clive Burr (1957–2013), British musician, former drummer with Iron Maiden * Clive Campbell (footballer), New Zealand footballer in the 1970s and early '80s * Clive Campbell (born 1955), Jamaican-born DJ with the stage name DJ Kool Herc * Clive Clark (golfer) (born 1945), English golfer * Clive Clark (footballer) (1940–2014), English former footballer ...
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Molecular Biology
Molecular biology is the branch of biology that seeks to understand the molecular basis of biological activity in and between cells, including biomolecular synthesis, modification, mechanisms, and interactions. The study of chemical and physical structure of biological macromolecules is known as molecular biology. Molecular biology was first described as an approach focused on the underpinnings of biological phenomena - uncovering the structures of biological molecules as well as their interactions, and how these interactions explain observations of classical biology. In 1945 the term molecular biology was used by physicist William Astbury. In 1953 Francis Crick, James Watson, Rosalind Franklin, and colleagues, working at Medical Research Council unit, Cavendish laboratory, Cambridge (now the MRC Laboratory of Molecular Biology), made a double helix model of DNA which changed the entire research scenario. They proposed the DNA structure based on previous research done by Ro ...
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China
China, officially the People's Republic of China (PRC), is a country in East Asia. It is the world's most populous country, with a population exceeding 1.4 billion, slightly ahead of India. China spans the equivalent of five time zones and borders fourteen countries by land, the most of any country in the world, tied with Russia. Covering an area of approximately , it is the world's third largest country by total land area. The country consists of 22 provinces, five autonomous regions, four municipalities, and two Special Administrative Regions (Hong Kong and Macau). The national capital is Beijing, and the most populous city and financial center is Shanghai. Modern Chinese trace their origins to a cradle of civilization in the fertile basin of the Yellow River in the North China Plain. The semi-legendary Xia dynasty in the 21st century BCE and the well-attested Shang and Zhou dynasties developed a bureaucratic political system to serve hereditary monarchies, or dyna ...
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Spore
In biology, a spore is a unit of sexual or asexual reproduction that may be adapted for dispersal and for survival, often for extended periods of time, in unfavourable conditions. Spores form part of the life cycles of many plants, algae, fungi and protozoa. Bacterial spores are not part of a sexual cycle, but are resistant structures used for survival under unfavourable conditions. Myxozoan spores release amoeboid infectious germs ("amoebulae") into their hosts for parasitic infection, but also reproduce within the hosts through the pairing of two nuclei within the plasmodium, which develops from the amoebula. In plants, spores are usually haploid and unicellular and are produced by meiosis in the sporangium of a diploid sporophyte. Under favourable conditions the spore can develop into a new organism using mitotic division, producing a multicellular gametophyte, which eventually goes on to produce gametes. Two gametes fuse to form a zygote which develops into a new s ...
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Real-time PCR
A real-time polymerase chain reaction (real-time PCR, or qPCR) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used quantitatively (quantitative real-time PCR) and semi-quantitatively (i.e., above/below a certain amount of DNA molecules) (semi-quantitative real-time PCR). Two common methods for the detection of PCR products in real-time PCR are (1) non-specific fluorescent dyes that intercalate with any double-stranded DNA and (2) sequence-specific DNA probes consisting of oligonucleotides that are labelled with a fluorescent reporter, which permits detection only after hybridization of the probe with its complementary sequence. The Minimum Information for Publication of Quantitative Real-Time PCR Experiments ( MIQE) guidelines propose that the abbreviation ''qPCR'' be used for q ...
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Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications ...
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DNA Extraction
The first isolation of deoxyribonucleic acid (DNA) was done in 1869 by Friedrich Miescher. Currently, it is a routine procedure in molecular biology or forensic analyses. For the chemical method, many different kits are used for extraction, and selecting the correct one will save time on kit optimization and extraction procedures. PCR sensitivity detection is considered to show the variation between the commercial kits. What does it deliver? DNA extraction is frequently a preliminary step in many diagnostic procedures used to identify environmental viruses and bacteria and diagnose illnesses and hereditary diseases. These methods consist of, but are not limited to: Fluorescence In Situ Hybridization (FISH) technique was developed in the 1980s. The basic idea is to use a nucleic acid probe to hybridize nuclear DNA from either interphase cells or metaphase chromosomes attached to a microscopic slide. It is a molecular method used, among other things, to recognize and count parti ...
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Canker
A plant canker is a small area of dead tissue, which grows slowly, often over years. Some cankers are of only minor consequence, but others are ultimately lethal and therefore can have major economic implications for agriculture and horticulture. Their causes include a wide range of organisms as fungi, bacteria, mycoplasmas and viruses. The majority of canker-causing organisms are bound to a unique host species or genus, but a few will attack other plants. Weather and animals can spread canker, thereby endangering areas that have only slight amount of canker. Although fungicides or bactericides can treat some cankers, often the only available treatment is to destroy the infected plant to contain the disease. Examples * Apple canker, caused by the fungus ''Neonectria galligena'' * Ash bacterial canker, now understood to be caused by the bacterium '' Pseudomonas savastanoi'', rather than ''Pseudomonas syringae''. After DNA-relatedness studies ''Pseudomonas savastanoi'' has bee ...
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Necrotic
Necrosis () is a form of cell injury which results in the premature death of cells in living tissue by autolysis. Necrosis is caused by factors external to the cell or tissue, such as infection, or trauma which result in the unregulated digestion of cell components. In contrast, apoptosis is a naturally occurring programmed and targeted cause of cellular death. While apoptosis often provides beneficial effects to the organism, necrosis is almost always detrimental and can be fatal. Cellular death due to necrosis does not follow the apoptotic signal transduction pathway, but rather various receptors are activated and result in the loss of cell membrane integrity and an uncontrolled release of products of cell death into the extracellular space. This initiates in the surrounding tissue an inflammatory response, which attracts leukocytes and nearby phagocytes which eliminate the dead cells by phagocytosis. However, microbial damaging substances released by leukocytes would crea ...
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Petiole (botany)
In botany, the petiole () is the stalk that attaches the leaf blade to the stem, and is able to twist the leaf to face the sun. This gives a characteristic foliage arrangement to the plant. Outgrowths appearing on each side of the petiole in some species are called stipules. Leaves with a petiole are said to be petiolate, while leaves lacking a petiole are called sessile or apetiolate. Description The petiole is a stalk that attaches a leaf to the plant stem. In petiolate leaves, the leaf stalk may be long, as in the leaves of celery and rhubarb, or short. When completely absent, the blade attaches directly to the stem and is said to be sessile. Subpetiolate leaves have an extremely short petiole, and may appear sessile. The broomrape family Orobanchaceae is an example of a family in which the leaves are always sessile. In some other plant groups, such as the speedwell genus '' Veronica'', petiolate and sessile leaves may occur in different species. In the grasses (Poaceae), ...
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Rhododendron
''Rhododendron'' (; from Ancient Greek ''rhódon'' "rose" and ''déndron'' "tree") is a very large genus of about 1,024 species of woody plants in the heath family (Ericaceae). They can be either evergreen or deciduous. Most species are native to eastern Asia and the Himalayan region, but smaller numbers occur elsewhere in Asia, and in North America, Europe and Australia. It is the national flower of Nepal, the state flower of Washington and West Virginia in the United States, the state flower of Nagaland in India, the provincial flower of Jiangxi in China and the state tree of Sikkim and Uttarakhand in India. Most species have brightly colored flowers which bloom from late winter through to early summer. Azaleas make up two subgenera of ''Rhododendron''. They are distinguished from "true" rhododendrons by having only five anthers per flower. Species Description ''Rhododendron'' is a genus of shrubs and small to (rarely) large trees, the smallest species growing to t ...
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Shrub
A shrub (often also called a bush) is a small-to-medium-sized perennial woody plant. Unlike herbaceous plants, shrubs have persistent woody stems above the ground. Shrubs can be either deciduous or evergreen. They are distinguished from trees by their multiple stems and shorter height, less than tall. Small shrubs, less than 2 m (6.6 ft) tall are sometimes termed as subshrubs. Many botanical groups have species that are shrubs, and others that are trees and herbaceous plants instead. Some definitions state that a shrub is less than and a tree is over 6 m. Others use as the cut-off point for classification. Many species of tree may not reach this mature height because of hostile less than ideal growing conditions, and resemble a shrub-sized plant. However, such species have the potential to grow taller under the ideal growing conditions for that plant. In terms of longevity, most shrubs fit in a class between perennials and trees; some may only last about five y ...
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