Mycobacterium Gordonae
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Mycobacterium Gordonae
''Mycobacterium gordonae'' is a species of ''Mycobacterium'' named for Ruth E. Gordon. It is a species of the phylum Actinomycetota (Gram-positive bacteria with high guanine and cytosine content, one of the dominant phyla of all bacteria), belonging to the genus ''Mycobacterium''. Description Gram-positive, nonmotile and moderate to long acid-fast rods. *Commonly found in tap water and soil. Casual resident in human sputum and gastric lavage specimens. Colony characteristics *Smooth, with yellow or orange scotochromogenic colonies. Even though they are scotochromogenic pigment is intensified by growing in continuous light. Physiology *Growth on Löwenstein-Jensen medium and Middlebrook 7H10 agar within 7 or more days at 37 °C (optimal 25 °C). *Does not grow in the presence of ethambutol (1 mg/L), isoniazid (10 mg/L) and sodium chloride (5%). *Some strains can grow using carbon monoxide as a carbon and energy source. Differential characteristics *A c ...
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Mycobacterium
''Mycobacterium'' is a genus of over 190 species in the phylum Actinomycetota, assigned its own family, Mycobacteriaceae. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis ('' M. tuberculosis'') and leprosy ('' M. leprae'') in humans. The Greek prefix ''myco-'' means 'fungus', alluding to this genus' mold-like colony surfaces. Since this genus has cell walls with Gram-positive and Gram-negative features, acid-fast staining is used to emphasize their resistance to acids, compared to other cell types. Metabolism and Morphology Mycobacteria are aerobic with 0.2-0.6 µm wide and 1.0-10 µm long rod shapes. They are generally non-motile, except for the species ''Mycobacterium marinum'', which has been shown to be motile within macrophages. Mycobacteria possess capsules and most do not form endospores. ''M. marinum'' and perhaps ''M. bovis'' have been shown to sporulate; however, this has been contested by further research. The disti ...
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Acid-fast Bacilli
Acid-fastness is a physical property of certain bacterial and eukaryotic cells, as well as some sub-cellular structures, specifically their resistance to decolorization by acids during laboratory staining procedures. Once stained as part of a sample, these organisms can resist the acid and/or ethanol-based decolorization procedures common in many staining protocols, hence the name ''acid-fast''. The mechanisms of acid-fastness vary by species, although the most well-known example is in the genus ''Mycobacterium'', which includes the species responsible for tuberculosis and leprosy. The acid-fastness of ''Mycobacteria'' is due to the high mycolic acid content of their cell walls, which is responsible for the staining pattern of poor absorption followed by high retention. Some bacteria may also be partially acid-fast, such as ''Nocardia''. Acid-fast organisms are difficult to characterize using standard microbiological techniques, though they can be stained using concentrated dyes, ...
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Immunocompromised
Immunodeficiency, also known as immunocompromisation, is a state in which the immune system's ability to fight infectious diseases and cancer is compromised or entirely absent. Most cases are acquired ("secondary") due to extrinsic factors that affect the patient's immune system. Examples of these extrinsic factors include HIV infection and environmental factors, such as nutrition. Immunocompromisation may also be due to genetic diseases/flaws such as SCID. In clinical settings, immunosuppression by some drugs, such as steroids, can either be an adverse effect or the intended purpose of the treatment. Examples of such use is in organ transplant surgery as an anti- rejection measure and in patients with an overactive immune system, as in autoimmune diseases. Some people are born with intrinsic defects in their immune system, or primary immunodeficiency. A person who has an immunodeficiency of any kind is said to be immunocompromised. An immunocompromised individual may particular ...
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16S RDNA
16 S ribosomal RNA (or 16 S rRNA) is the RNA component of the 30S subunit of a prokaryotic ribosome (SSU rRNA). It binds to the Shine-Dalgarno sequence and provides most of the SSU structure. The genes coding for it are referred to as 16S rRNA gene and are used in reconstructing phylogenies, due to the slow rates of evolution of this region of the gene. Carl Woese and George E. Fox were two of the people who pioneered the use of 16S rRNA in phylogenetics in 1977. Multiple sequences of the 16S rRNA gene can exist within a single bacterium. Functions * Like the large (23S) ribosomal RNA, it has a structural role, acting as a scaffold defining the positions of the ribosomal proteins. * The 3-end contains the anti- Shine-Dalgarno sequence, which binds upstream to the AUG start codon on the mRNA. The 3-end of 16S RNA binds to the proteins S1 and S21 which are known to be involved in initiation of protein synthesis * Interacts with 23S, aiding in the binding of the two ribosom ...
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Nucleic Acid Hybridization
Nucleus ( : nuclei) is a Latin word for the seed inside a fruit. It most often refers to: *Atomic nucleus, the very dense central region of an atom *Cell nucleus, a central organelle of a eukaryotic cell, containing most of the cell's DNA Nucleus may also refer to: Science, technology, and mathematics Astronomy *Active galactic nucleus in astronomy *Comet nucleus, the solid, central part of a comet Biology *Cell nucleus, a central organelle of a eukaryotic cell, containing most of the cell's DNA *Nucleus (neuroanatomy), a cluster of cell bodies of neurons in the central nervous system *Nucleus that forms in the eye in nuclear sclerosis (early cataracts) *''Nucleus'', a scientific journal concerned with the cell nucleus; published by Taylor & Francis *Nucleus, a small colony of honeybees, induced to raise a new queen by the beekeeper Computer systems * Nucleus (operating system), sometimes a synonym for kernel * Nucleus CMS, a weblog system * Nucleus RTOS, a real-time oper ...
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Middlebrook 7H10 Agar
Middlebrook 7H10 Agar is a solid growth medium specially used for culture of ''Mycobacterium'', notably ''Mycobacterium tuberculosis''. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria. Composition Ingredients (g/L) * Ammonium sulfate, 0.50 * Monopotassium phosphate, 1.50 * Disodium phosphate, 1.50 * Sodium citrate, 0.40 * Magnesium sulfate, 0.025 * Zinc sulfate, 0.001 * Copper sulfate, 0.001 * L-Glutamic acid, 0.50 * Ferric ammonium citrate, 0.04 * Pyridoxine hydrochloride, 0.001 * Biotin, 0.0005 * Malachite green, 0.00025 * Agar, 15.00 * Calcium chloride, 0.0005 Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks. See also * Lowenstein-Jensen medium * Middlebrook 7H9 Broth Middlebrook 7H9 broth is a liquid growth medium specially used for culture of'' Mycobacterium'' species, notably '' Mycobacterium tuberculosis''. Compo ...
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Scotochromogenic
Scotochromogenic bacteria develop pigment in the dark. Runyon Group II nontuberculous mycobacteria such as Mycobacterium gordonae ''Mycobacterium gordonae'' is a species of '' Mycobacterium'' named for Ruth E. Gordon. It is a species of the phylum Actinomycetota (Gram-positive bacteria with high guanine and cytosine content, one of the dominant phyla of all bacteria), b ... are examples but the term could apply to many other organisms. References Bacteria {{Bacteria-stub ...
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Ruth E
Ruth (or its variants) may refer to: Places France * Château de Ruthie, castle in the commune of Aussurucq in the Pyrénées-Atlantiques département of France Switzerland * Ruth, a hamlet in Cologny United States * Ruth, Alabama * Ruth, Arkansas * Ruth, California * Ruth, Louisiana * Ruth, Pulaski County, Kentucky * Ruth, Michigan * Ruth, Mississippi * Ruth, Nevada * Ruth, North Carolina * Ruth, Virginia * Ruth, Washington * Ruth, West Virginia In space * Ruth (lunar crater), crater on the Moon * Ruth (Venusian crater), crater on Venus * 798 Ruth, asteroid People * Ruth (biblical figure) * Ruth (given name) contains list of namesakes including fictional * Princess Ruth or Keʻelikōlani, (1826–1883), Hawaiian princess Surname * A. S. Ruth, American politician * Babe Ruth (1895–1948), American baseball player * Connie Ruth, American politician * Earl B. Ruth (1916–1989), American politician * Elizabeth Ruth, Canadian novelist * Kristin Ruth, American judge * Nanc ...
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Gastric Lavage
Gastric lavage, also commonly called stomach pumping or gastric irrigation, is the process of cleaning out the contents of the stomach. Since its first recorded use in early 19th century, it has become one of the most routine means of eliminating poisons from the stomach. Such devices are normally used on a person who has ingested a poison or overdosed on a drug such as ethanol. They may also be used before surgery, to clear the contents of the digestive tract before it is opened. Apart from toxicology, gastric lavage (or nasogastric lavage) is sometimes used to confirm levels of bleeding from the upper gastrointestinal tract. It may play a role in the evaluation of hematemesis. It can also be used as a cooling technique for hyperthermic patients. Technique Gastric lavage involves the passage of a tube (such as an ''Ewald tube'') via the mouth or nose down into the stomach followed by sequential administration and removal of small volumes of liquid. The placement of the tube in ...
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