Middlebrook 7H10 Agar is a solid growth medium specially used for culture of ''Mycobacterium'', notably ''

Mycobacterium tuberculosis ''Mycobacterium tuberculosis'' (M. tb) is a species of pathogenic bacteria in the family Mycobacteriaceae and the causative agent of tuberculosis. First discovered in 1882 by Robert Koch, ''M. tuberculosis'' has an unusual, waxy coating on its c ...

''. It has been reported that the 7H10 medium tends to grow fewer contaminants than the egg-based media commonly used for the cultivation of mycobacteria.

Composition

Ingredients (g/L) *

Ammonium sulfate Ammonium sulfate (American English and international scientific usage; ammonium sulphate in British English); (NH4)2SO4, is an inorganic salt with a number of commercial uses. The most common use is as a soil fertilizer. It contains 21% nitrogen a ...

, 0.50 *

Monopotassium phosphate Monopotassium phosphate (MKP) (also, potassium dihydrogenphosphate, KDP, or monobasic potassium phosphate) is the inorganic compound with the formula KH2PO4. Together with dipotassium phosphate (K2HPO4.(H2O)x) it is often used as a fertilizer, f ...

, 1.50 *

Disodium phosphate Disodium phosphate (DSP), or disodium hydrogen phosphate, or sodium phosphate dibasic, is the inorganic compound with the formula Na2HPO4. It is one of several sodium phosphates. The salt is known in anhydrous form as well as forms with 2, 7, 8, ...

, 1.50 *

Sodium citrate Sodium citrate may refer to any of the sodium salts of citric acid (though most commonly the third): * Monosodium citrate * Disodium citrate * Trisodium citrate The three forms of salt are collectively known by the E number E331. Applications F ...

, 0.40 *

Magnesium sulfate Magnesium sulfate or magnesium sulphate (in English-speaking countries other than the US) is a chemical compound, a salt with the formula , consisting of magnesium cations (20.19% by mass) and sulfate anions . It is a white crystalline solid, ...

, 0.025 *

Zinc sulfate Zinc sulfate is an inorganic compound. It is used as a dietary supplement to treat zinc deficiency and to prevent the condition in those at high risk. Side effects of excess supplementation may include abdominal pain, vomiting, headache, and ti ...

, 0.001 *

Copper sulfate Copper sulfate may refer to: * Copper(II) sulfate, CuSO4, a common compound used as a fungicide and herbicide * Copper(I) sulfate Copper(I) sulfate, also known as cuprous sulfate, is an inorganic compound with the chemical formula Cu2 SO4. It ...

, 0.001 * L-Glutamic acid, 0.50 *

Ferric ammonium citrate Ammonium ferric citrate (also known as Ferric Ammonium Citrate or Ammoniacal ferrous citrate) has the formula (NH4)5 e(C6H4O7)2 A distinguishing feature of this compound is that it is very soluble in water, in contrast to ferric citrate which is ...

, 0.04 *

Pyridoxine hydrochloride Pyridoxine, is a form of vitamin B6 found commonly in food and used as a dietary supplement. As a supplement it is used to treat and prevent pyridoxine deficiency, sideroblastic anaemia, pyridoxine-dependent epilepsy, certain metabolic disor ...

, 0.001 *

Biotin Biotin (or vitamin B7) is one of the B vitamins. It is involved in a wide range of metabolic processes, both in humans and in other organisms, primarily related to the utilization of fats, carbohydrates, and amino acids. The name ''biotin'', bor ...

, 0.0005 *

Malachite green Malachite green is an organic compound that is used as a dyestuff and controversially as an antimicrobial in aquaculture. Malachite green is traditionally used as a dye for materials such as silk, leather, and paper. Despite its name the dye is ...

, 0.00025 *

Agar Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is ...

, 15.00 *

Calcium chloride Calcium chloride is an inorganic compound, a salt with the chemical formula . It is a white crystalline solid at room temperature, and it is highly soluble in water. It can be created by neutralising hydrochloric acid with calcium hydroxide. Ca ...

, 0.0005 Cultures should be read within 5–7 days after inoculation and once a week thereafter for up to 8 weeks.

References

External links

Middlebrook 7H10 Agar
{{Growth media * http://www.bd.com/ds/productCenter/295964.asp Microbiological media

Composition

See also

References

External links