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Genetically Encoded Voltage Indicator
Genetically encoded voltage indicator (or GEVI) is a protein that can sense membrane potential in a cell and relate the change in voltage to a form of output, often fluorescent level. It is a promising optogenetic recording tool that enables exporting electrophysiological signals from cultured cells, live animals, and ultimately human brain. Examples of notable GEVIs include ArcLight, ASAP1, ASAP3, Archons, SomArchon, and Ace2N-mNeon. History Despite that the idea of optical measurement of neuronal activity was proposed in the late 1960s, the first successful GEVI that was convenient enough to put into actual use was not developed until technologies of genetic engineering had become mature in the late 1990s. The first GEVI, coined FlaSh, was constructed by fusing a modified green fluorescent protein with a voltage-sensitive K+ channel ( Shaker). Unlike fluorescent proteins, the discovery of new GEVIs were seldom inspired by the nature, for it is hard to find an organism which ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, responding to stimuli, providing structure to cells and organisms, and transporting molecules from one location to another. Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific 3D structure that determines its activity. A linear chain of amino acid residues is called a polypeptide. A protein contains at least one long polypeptide. Short polypeptides, containing less than 20–30 residues, are rarely considered to be proteins and are commonly called peptides. The individual amino acid residues are bonded together by peptide bonds and adjacent amino acid residues. The sequence of amino acid residue ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genomics
Genomics is an interdisciplinary field of biology focusing on the structure, function, evolution, mapping, and editing of genomes. A genome is an organism's complete set of DNA, including all of its genes as well as its hierarchical, three-dimensional structural configuration. In contrast to genetics, which refers to the study of ''individual'' genes and their roles in inheritance, genomics aims at the collective characterization and quantification of ''all'' of an organism's genes, their interrelations and influence on the organism. Genes may direct the production of proteins with the assistance of enzymes and messenger molecules. In turn, proteins make up body structures such as organs and tissues as well as control chemical reactions and carry signals between cells. Genomics also involves the sequencing and analysis of genomes through uses of high throughput DNA sequencing and bioinformatics to assemble and analyze the function and structure of entire genomes. Advances in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Journal Of Neurophysiology
The ''Journal of Neurophysiology'' is a monthly peer-reviewed scientific journal established in 1938. It is published by the American Physiological Society with Jan "Nino" Ramirez as its editor-in-chief. Ramirez is the Director for the Center for Integrative Brain Research at the University of Washington. The ''Journal of Neurophysiology'' publishes original articles on the function of the nervous system. All levels of function are included, from membrane biophysics to cell biology to systems neuroscience and the experimental analysis of behavior. Experimental approaches include molecular neurobiology, cell culture and slice preparations, membrane physiology, developmental neurobiology, functional neuroanatomy, neurochemistry, neuropharmacology, systems electrophysiology, imaging and mapping techniques, and behavioral analysis. Experimental preparations may be invertebrate or vertebrate species, including humans. Theoretical studies are acceptable if they are tied closely to t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Archaearhodopsin
Bacteriorhodopsin is a protein used by Archaea, most notably by haloarchaea, a class of the Euryarchaeota. It acts as a proton pump; that is, it captures light energy and uses it to move protons across the membrane out of the cell. The resulting proton gradient is subsequently converted into chemical energy. Function Bacteriorhodopsin is a light-driven H+ ion transporter found in some haloarchaea, most notably ''Halobacterium salinarum'' (formerly known as syn. ''H. halobium''). The proton-motive force generated by the protein is used by ATP synthase to generate adenosine triphosphate (ATP). By expressing bacteriorhodopsin, the archaea cells are able to synthesise ATP in the absence of a carbon source. Structure Bacteriorhodopsin is a 27 kDa integral membrane protein usually found in two-dimensional crystalline patches known as "purple membrane", which can occupy almost 50% of the surface area of the archaeal cell. The repeating element of the hexagonal lattice is composed o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Arclight (biology)
ArcLight is a genetically-encoded voltage indicator (GEVI) created from Ciona intestinalis voltage sensor and the fluorescent protein super ecliptic pHluorin that carries a critical point mutation A point mutation is a genetic mutation where a single nucleotide base is changed, inserted or deleted from a DNA or RNA sequence of an organism's genome. Point mutations have a variety of effects on the downstream protein product—consequences ... (A227D). References Electrophysiology {{biotech-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Science (journal)
''Science'', also widely referred to as ''Science Magazine'', is the peer-reviewed academic journal of the American Association for the Advancement of Science (AAAS) and one of the world's top academic journals. It was first published in 1880, is currently circulated weekly and has a subscriber base of around 130,000. Because institutional subscriptions and online access serve a larger audience, its estimated readership is over 400,000 people. ''Science'' is based in Washington, D.C., United States, with a second office in Cambridge, UK. Contents The major focus of the journal is publishing important original scientific research and research reviews, but ''Science'' also publishes science-related news, opinions on science policy and other matters of interest to scientists and others who are concerned with the wide implications of science and technology. Unlike most scientific journals, which focus on a specific field, ''Science'' and its rival ''Nature (journal), Nature'' c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemistry & Biology
''Cell Chemical Biology'' is a monthly, peer-reviewed, scientific journal published by Cell Press. The journal publishes research in chemical biology and studies at the interface of chemistry and biology Biology is the scientific study of life. It is a natural science with a broad scope but has several unifying themes that tie it together as a single, coherent field. For instance, all organisms are made up of cells that process hereditary i .... References External links * Biochemistry journals Cell Press academic journals Monthly journals {{biochemistry-journal-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dipicrylamine
Hexanitrodiphenylamine (abbreviated HND), is an explosive chemical compound with the formula C12H5N7O12. HND was used extensively by the Empire of Japan, Japanese during World War II but was discontinued due to its toxicity. Preparation Dinitrodiphenylamine is treated with 98% nitric acid. The starting material, dinitrodiphenylamine, is obtained from the reaction of aniline, dinitrochlorobenzene, and soda ash. Applications HND is a booster-class explosive that was used in World War II by the Nazi Germany, Germans as a component of Hexanite (60% TNT - 40% HND) and by the Japanese as a component of Kongo (Type 98 H2) (60% Trinitroanisole - 40% HND) for use in bombs, sea mines and depth charges; Seigate (Type 97 H) (60% TNT - 40% HND) for use in torpedo warheads and depth charges; and also in Otsu-B (60% TNT, 24% HND & 16% aluminium powder) for use in torpedo warheads. Its ammonium salt, also known as Aurantia or Imperial Yellow, was discovered in 1873 by Emil Kopp and used as a ye ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
The Journal Of Neuroscience
''The Journal of Neuroscience'' is a weekly peer-reviewed scientific journal published by the Society for Neuroscience. It covers empirical research on all aspects of neuroscience. Its editor-in-chief is Marina Picciotto (Yale University). According to the ''Journal Citation Reports'', the journal has a 2020 impact factor of 6.167. History The journal was established in 1981 and issues appeared monthly; as its popularity grew it switched to a biweekly schedule in 1996 and then to a weekly in July 2003. Themes Main themes Articles appear within one of the following five sections of the journal: * Cellular/Molecular * Development/Plasticity/Repair * Systems/Circuits * Behavioral/Cognitive * Neurobiology of Disease The journal has revised its sections over the years. In 2004, it added the Neurobiology of Disease section due to the growing number of papers on this subject. In January 2013, the journal split the section Behavioral/Systems/Cognitive into two sections, Systems/Circ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nature Methods
''Nature Methods'' is a monthly peer-reviewed scientific journal covering new scientific techniques. It was established in 2004 and is published by Springer Nature under the Nature Portfolio. Like other ''Nature'' journals, there is no external editorial board and editorial decisions are made by an in-house team, although peer review by external experts forms a part of the review process. Every year, the journal highlights a field, approach, or technique that has enabled recent major advances in life sciences research as the "Method of the Year". According to the ''Journal Citation Reports'', the journal had a 2021 impact factor The impact factor (IF) or journal impact factor (JIF) of an academic journal is a scientometric index calculated by Clarivate that reflects the yearly mean number of citations of articles published in the last two years in a given journal, as i ... of 47.99, ranking it first in the category "Biochemical Research Methods". References External link ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Journal Of Neuroscience Methods
The ''Journal of Neuroscience Methods'' is a peer-reviewed scientific journal covering scientific techniques and protocols used in any branch of neuroscience research. It was established in 1979. The editor-in-chief is Giuseppe Di Giovanni, Professor of Neuroscience at the University of Malta and Honorary Professor at Cardiff University, UK. Journal Neuroscience Methods is published 18 times a year by Elsevier. Associate Editors: Vince Calhoun ( The Mind Research Network, Albuquerque, New Mexico, USA), David Carter (Cardiff University, Cardiff, UK), Philippe De Deurwaerdère (Université de Bordeaux, Bordeaux Cedex, France), Robert Hampson (Wake Forest University Health Sciences, Winston-Salem, North Carolina, USA), Liset Menendez de la Prida (Consejo Superior de Investigaciones Científicas (CSIC), Madrid, Spain), Gernot Riedel (University of Aberdeen, Foresterhill, Scotland, UK), Floris Wouterlood (Vrije Universiteit Amsterdam, Amsterdam, Netherlands). Previous editors in ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
