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Formatotrophs
Formatotrophs are organisms that can assimilate formate or formic acid to use as a carbon source or for reducing power. Some authors classify formatotrophs as one of the five trophic groups of methanogens, which also include hydrogenotrophs, acetotrophs, methylotrophs, and alcoholotrophs. Formatotrophs have garnered attention for applications in biotechnology as part of a "formate bioeconomy" in which synthesized formate could be used as a nutrient for microoganisms. Formate can be electrochemically synthesized from CO2 and renewable energy, and formatotrophs may be genetically modified to enhance production of biochemical products to be used as biofuels. Technical limitations in culturing formatotrophs have limited the discovery of natural formatotrophs and impeded research on their formate-metabolizing enzymes, which are of interest for applications in carbon sequestration and astrobiology. Etymology Formatotrophs gain their name from Latin ''formica'', meaning "ant" (formic ac ...
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Methylorubrum Extorquens
''Methylorubrum extorquens'' is a Gram-negative bacterium. ''Methylorubrum'' species often appear pink, and are classified as pink-pigmented facultative methylotrophs, or PPFMs. The wild type has been known to use both methane and multiple carbon compounds as energy sources. Specifically, ''M. extorquens'' has been observed to use primarily methanol anC1 compoundsas substrates in their energy cycles. It has been also observed that use lanthanides as a cofactor to increase its methanol dehydrogenase activity Genetic structure After isolation from soil, ''M. extorquens'' was found to have a single chromosome measuring 5.71- Mb. The bacterium itself contains 70 genes over eight regions of the chromosome that are used for its metabolism of methanol. Within a section of the chromosome, of ''M. extorquens'' AM1 are twxoxFgenes that enable it to grow in methanol. ''M. extorquens'' AM1 genome encodes a 47.5 kb gene of unknown function. This gene encodes an over 15,000 residue-long po ...
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Methanogen
Methanogens are microorganisms that produce methane as a metabolic byproduct in hypoxic conditions. They are prokaryotic and belong to the domain Archaea. All known methanogens are members of the archaeal phylum Euryarchaeota. Methanogens are common in wetlands, where they are responsible for marsh gas, and in the digestive tracts of animals such as ruminants and many humans, where they are responsible for the methane content of belching in ruminants and flatulence in humans. In marine sediments, the biological production of methane, also termed methanogenesis, is generally confined to where sulfates are depleted, below the top layers. Moreover, methanogenic archaea populations play an indispensable role in anaerobic wastewater treatments. Others are extremophiles, found in environments such as hot springs and submarine hydrothermal vents as well as in the "solid" rock of Earth's crust, kilometers below the surface. Physical description Methanogens are coccoid (spherical shap ...
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Cupriavidus Necator
''Cupriavidus necator'' is a Gram-negative soil bacterium of the class Betaproteobacteria. Taxonomy ''Cupriavidus necator'' has gone through a series of name changes. In the first half of the 20th century, many micro-organisms were isolated for their ability to use hydrogen. Hydrogen-metabolizing chemolithotrophic organisms were clustered into the group ''Hydrogenomonas''. ''C. necator'' was originally named ''Hydrogenomonas eutrophus'' because it fell under the ''Hydrogenomonas'' classification and was “well nourished and robust”. Some of the original ''H. eutrophus'' cultures isolated were by Bovell and Wilde. After characterizing cell morphology, metabolism and GC content, the ''Hydrogenomonas'' nomenclature was disbanded because it comprised many species of microorganisms. ''H. eutrophus'' was then renamed ''Alcaligenes eutropha'' because it was a micro-organism with degenerated peritrichous flagellation. Investigating phenotype, lipid composition, fatty acid com ...
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Desulfurococcus Amylolyticus
In taxonomy, ''Desulfurococcus'' is a genus of the Desulfurococcaceae.See the NCBI The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH). It is approved and funded by the government of the United States. The ...br>webpage on Desulfurococcus Data extracted from the References Further reading Scientific journals * * * Scientific databases External links Type strain of ''Desulfurococcus mobilis'' at Bac''Dive'' - the Bacterial Diversity Metadatabase Archaea genera Formatotrophs Thermoproteota {{archaea-stub ...
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Formate
Formate (IUPAC name: methanoate) is the conjugate base of formic acid. Formate is an anion () or its derivatives such as ester of formic acid. The salts and esters are generally colorless.Werner Reutemann and Heinz Kieczka "Formic Acid" in ''Ullmann's Encyclopedia of Industrial Chemistry'' 2002, Wiley-VCH, Weinheim. Fundamentals When dissolved in water, formic acid converts to formate: : Formate is a planar anion. The two oxygen atoms are equivalent and bear a partial negative charge. The remaining C-H bond is not acidic. Biochemistry : Formate is a common C-1 source in living systems. It is formed from many precursors including choline, serine, and sarcosine. It provides a C-1 source in the biosynthesis of some nucleic acids. Formate (or formic acid) is ina leaving group in the demethylation of some sterols.. These conversions are catalyzed by aromatase enzymes using O2 as the oxidant. Specific conversions include testosterone to estradiol and androstenedione to estrone ...
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Bicarbonate
In inorganic chemistry, bicarbonate (IUPAC-recommended nomenclature: hydrogencarbonate) is an intermediate form in the deprotonation of carbonic acid. It is a polyatomic anion with the chemical formula . Bicarbonate serves a crucial biochemical role in the physiological pH buffering system. The term "bicarbonate" was coined in 1814 by the English chemist William Hyde Wollaston. The name lives on as a trivial name. Chemical properties The bicarbonate ion (hydrogencarbonate ion) is an anion with the empirical formula and a molecular mass of 61.01  daltons; it consists of one central carbon atom surrounded by three oxygen atoms in a trigonal planar arrangement, with a hydrogen atom attached to one of the oxygens. It is isoelectronic with nitric acid . The bicarbonate ion carries a negative one formal charge and is an amphiprotic species which has both acidic and basic properties. It is both the conjugate base of carbonic acid ; and the conjugate acid of , ...
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Serpentinization
Serpentinization is a hydration and Metamorphic rock, metamorphic transformation of ferromagnesian minerals, such as olivine and pyroxene, in mafic and ultramafic rock to produce serpentinite. Minerals formed by serpentinization include the Serpentine subgroup, serpentine group minerals (antigorite, lizardite, chrysotile), brucite, talc, Ni-Fe alloys, and magnetite. The mineral alteration is particularly important at the sea floor at plate tectonics, tectonic plate boundaries. Formation and petrology Serpentinization is a form of low-temperature (0 to ~600 °C) metamorphism of ferromagnesian minerals in mafic and ultramafic rocks, such as dunite, harzburgite, or lherzolite. These are rocks low in silica and composed mostly of olivine (), pyroxene (), and chromite (approximately ). Serpentinization is driven largely by Mineral hydration, hydration and oxidation of olivine and pyroxene to serpentine subgroup, serpentine group minerals (antigorite, lizardite, and chrysotile), bru ...
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Atlantic Ocean
The Atlantic Ocean is the second-largest of the world's five oceans, with an area of about . It covers approximately 20% of Earth's surface and about 29% of its water surface area. It is known to separate the " Old World" of Africa, Europe and Asia from the "New World" of the Americas in the European perception of the World. The Atlantic Ocean occupies an elongated, S-shaped basin extending longitudinally between Europe and Africa to the east, and North and South America to the west. As one component of the interconnected World Ocean, it is connected in the north to the Arctic Ocean, to the Pacific Ocean in the southwest, the Indian Ocean in the southeast, and the Southern Ocean in the south (other definitions describe the Atlantic as extending southward to Antarctica). The Atlantic Ocean is divided in two parts, by the Equatorial Counter Current, with the North(ern) Atlantic Ocean and the South(ern) Atlantic Ocean split at about 8°N. Scientific explorations of the A ...
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Lost City Hydrothermal Field
The Lost City Hydrothermal Field, often referred to simply as Lost City, is an area of marine alkaline hydrothermal vents located on the Atlantis Massif at the intersection between the Mid-Atlantic Ridge and the Atlantis Transform Fault, in the Atlantic Ocean. It is a long-lived site of active and inactive ultramafic-hosted serpentinization, abiotically producing many simple molecules such as methane and hydrogen which are fundamental to microbial life. As such it has generated scientific interest as a prime location for investigating the origin of life on Earth and other planets similar to it. Expedition history The Lost City was first identified on December 4, 2000, using ''DSV Alvin'' and ''ROV ArgoII'' on cruise AT03-60 of the RV Atlantis. The cruise lasted 34 days, during which photographs and vent chimney samples were taken. The discovery of the Lost City prompted the National Science Foundation to fund a second, 32-day voyage (AT07-34) to the site in 2003 in order to ...
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Metagenomics
Metagenomics is the study of genetic material recovered directly from environmental or clinical samples by a method called sequencing. The broad field may also be referred to as environmental genomics, ecogenomics, community genomics or microbiomics. While traditional microbiology and microbial genome sequencing and genomics rely upon cultivated clonal cultures, early environmental gene sequencing cloned specific genes (often the 16S rRNA gene) to produce a profile of diversity in a natural sample. Such work revealed that the vast majority of microbial biodiversity had been missed by cultivation-based methods. Because of its ability to reveal the previously hidden diversity of microscopic life, metagenomics offers a powerful lens for viewing the microbial world that has the potential to revolutionize understanding of the entire living world. As the price of DNA sequencing continues to fall, metagenomics now allows microbial ecology to be investigated at a much greater scale ...
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Confocal Laser Scanning Microscopy Images Showing Different Microbial Morphotyes In Chimney Samples
In geometry, confocal means having the same foci: confocal conic sections. * For an optical cavity consisting of two mirrors, confocal means that they share their foci. If they are identical mirrors, their radius of curvature, ''R''mirror, equals ''L'', where ''L'' is the distance between the mirrors. * In conic sections, it is said of two ellipses, two hyperbolas, or an ellipse and a hyperbola which share both foci with each other. If an ellipse and a hyperbola are confocal, they are perpendicular to each other. * In optics, it means that one focus or image point of one lens is the same as one focus of the next lens. See also *Confocal laser scanning microscopy *Confocal microscopy Confocal microscopy, most frequently confocal laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM), is an optical imaging technique for increasing optical resolution and contrast of a micrograph by means of using a sp ... * {{set index article, mathematics Elementary geo ...
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Ethanol
Ethanol (abbr. EtOH; also called ethyl alcohol, grain alcohol, drinking alcohol, or simply alcohol) is an organic compound. It is an Alcohol (chemistry), alcohol with the chemical formula . Its formula can be also written as or (an ethyl group linked to a hydroxyl group). Ethanol is a Volatility (chemistry), volatile, Combustibility and flammability, flammable, colorless liquid with a characteristic wine-like odor and pungent taste. It is a psychoactive recreational drug, the active ingredient in alcoholic drinks. Ethanol is naturally produced by the fermentation process of Carbohydrate, sugars by yeasts or via Petrochemistry, petrochemical processes such as ethylene hydration. It has medical applications as an antiseptic and disinfectant. It is used as a chemical solvent and in the Chemical synthesis, synthesis of organic compounds, and as a Alcohol fuel, fuel source. Ethanol also can be dehydrated to make ethylene, an important chemical feedstock. As of 2006, world produ ...
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