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Diazotroph
Diazotrophs are bacteria and archaea that fix gaseous nitrogen in the atmosphere into a more usable form such as ammonia. A diazotroph is a microorganism that is able to grow without external sources of fixed nitrogen. Examples of organisms that do this are rhizobia and ''Frankia'' (in symbiosis) and ''Azospirillum''. All diazotrophs contain iron-molybdenum or iron-vanadium nitrogenase systems. Two of the most studied systems are those of ''Klebsiella pneumoniae'' and ''Azotobacter vinelandii''. These systems are studied because of their genetic tractability and their fast growth. Etymology The word diazotroph is derived from the words ''diazo'' ("di" = two + "azo" = nitrogen) meaning "dinitrogen (N2)" and ''troph'' meaning "pertaining to food or nourishment", in summary dinitrogen utilizing. The word ''azote'' means nitrogen in French and was named by French chemist and biologist Antoine Lavoisier, who saw it as the part of air which cannot sustain life. Types of diazotrophs Diaz ...
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Nitrogen Fixation
Nitrogen fixation is a chemical process by which molecular nitrogen (), with a strong triple covalent bond, in the air is converted into ammonia () or related nitrogenous compounds, typically in soil or aquatic systems but also in industry. Atmospheric nitrogen is molecular dinitrogen, a relatively nonreactive molecule that is metabolically useless to all but a few microorganisms. Biological nitrogen fixation or ''diazotrophy'' is an important microbials mediated process that converts dinitrogen (N2) gas to ammonia (NH3) using the nitrogenase protein complex (Nif). Nitrogen fixation is essential to life because fixed inorganic nitrogen compounds are required for the biosynthesis of all nitrogen-containing organic compounds, such as amino acids and proteins, nucleoside triphosphates and nucleic acids. As part of the nitrogen cycle, it is essential for agriculture and the manufacture of fertilizer. It is also, indirectly, relevant to the manufacture of all nitrogen chemical c ...
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Trichodesmium
''Trichodesmium'', also called sea sawdust, is a genus of Filamentation, filamentous cyanobacteria. They are found in nutrient poor tropical and subtropical ocean waters (particularly around Australia and in the Red Sea, where they were first described by Captain Cook). ''Trichodesmium'' is a diazotroph; that is, it nitrogen fixation, fixes atmospheric nitrogen into ammonium, a nutrient used by other organisms. ''Trichodesmium'' is thought to fix nitrogen on such a scale that it accounts for almost half of the nitrogen fixation in marine systems globally. ''Trichodesmium'' is the only known diazotroph able to nitrogen fixation, fix nitrogen in daylight under aerobic conditions without the use of heterocysts. ''Trichodesmium'' can live as individual filaments, with tens to hundreds of cells strung together, or in colonies consisting of tens to hundreds of filaments clustered together. These colonies are visible to the naked eye and sometimes form blooms, which can be extensive on s ...
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Azospirillum
''Azospirillum'' is a Gram-negative, microaerophilic, non- fermentative and nitrogen-fixing bacterial genus from the family of Rhodospirillaceae. ''Azospirillum'' bacteria can promote plant growth. Characteristics The genus ''Azospirillum'' belongs in the alpha-Proteobacteria class of bacteria. ''Azospirillum'' are gram-negative, do not form spores, and have a slightly-twisted oblong-rod shape.  ''Azospirillum'' have at least one flagellum and sometimes multiple flagella, which they use to move rapidly. ''Azospirillum'' are aerobic, but many can also function as microaerobic diazotrophs, meaning, under low oxygen conditions, they can change inert nitrogen from the air into biologically useable forms. At least three species, ''A. melinis'', ''A. thiophilum'', and ''A. humicireducens'' are facultative anaerobes, and can live, if necessary, without oxygen. Growth of ''Azospirillum'' is possible between 5 °C and 42 °C and in substrates with a pH of 5 to 9, with optimal ...
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Rhizobia
Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). To express genes for nitrogen fixation, rhizobia require a plant host; they cannot independently fix nitrogen. In general, they are gram negative, motile, non-sporulating rods. Rhizobia are a "group of soil bacteria that infect the roots of legumes to form root nodules". Rhizobia are found in the soil and after infection, produce nodules in the legume where they fix nitrogen gas (N2) from the atmosphere turning it into a more readily useful form of nitrogen. From here, the nitrogen is exported from the nodules and used for growth in the legume. Once the legume dies, the nodule breaks down and releases the rhizobia back into the soil where they can live individually or reinfect a new legume host. History The first known species of rhizobia, '' Rhizobium leguminosarum'', was identified in 1889, and all further species were initially placed in the ''Rhiz ...
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Rhizobia
Rhizobia are diazotrophic bacteria that fix nitrogen after becoming established inside the root nodules of legumes (Fabaceae). To express genes for nitrogen fixation, rhizobia require a plant host; they cannot independently fix nitrogen. In general, they are gram negative, motile, non-sporulating rods. Rhizobia are a "group of soil bacteria that infect the roots of legumes to form root nodules". Rhizobia are found in the soil and after infection, produce nodules in the legume where they fix nitrogen gas (N2) from the atmosphere turning it into a more readily useful form of nitrogen. From here, the nitrogen is exported from the nodules and used for growth in the legume. Once the legume dies, the nodule breaks down and releases the rhizobia back into the soil where they can live individually or reinfect a new legume host. History The first known species of rhizobia, '' Rhizobium leguminosarum'', was identified in 1889, and all further species were initially placed in the ''Rhiz ...
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Cyanothece
''Cyanothece'' is a genus of unicellular, diazotrophic, oxygenic photosynthesizing cyanobacteria. Modern organisms and cellular organization In 1976, Jiří Komárek defined the prokaryotic cyanobacteria genus ''Cyanothece'' as distinct from ''Synechococcus'' NAG 1949. Organisms in both genera share characteristics in addition to being oxygenic phototrophs. They are both unicellular, forming aggregates, but not found in mucilaginous colonies. They may have a thin mucilage layer around each cell. Both genera also divide by binary fission along an axis perpendicular to the cell's longitudinal axis. A handful of characteristics distinguish the two genera. While ''Synechococcus'' species are usually cylindrical, ''Cyanothece'' species are normally oval and longer than 3 μm., ''Cyanothece’s'' outer cell wall layer is relatively thick and contains spherical, glassy Vesicle (biology and chemistry), vesicles whose function has yet to be defined. ''Cyanothece’s'' nucleoids ...
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Archaea
Archaea ( ; singular archaeon ) is a domain of single-celled organisms. These microorganisms lack cell nuclei and are therefore prokaryotes. Archaea were initially classified as bacteria, receiving the name archaebacteria (in the Archaebacteria kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from the other two domains, Bacteria and Eukaryota. Archaea are further divided into multiple recognized phyla. Classification is difficult because most have not been isolated in a laboratory and have been detected only by their gene sequences in environmental samples. Archaea and bacteria are generally similar in size and shape, although a few archaea have very different shapes, such as the flat, square cells of ''Haloquadratum walsbyi''. Despite this morphological similarity to bacteria, archaea possess genes and several metabolic pathways that are more closely related to those of eukaryotes, notably for the enzymes involved ...
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Cyanobacteria
Cyanobacteria (), also known as Cyanophyta, are a phylum of gram-negative bacteria that obtain energy via photosynthesis. The name ''cyanobacteria'' refers to their color (), which similarly forms the basis of cyanobacteria's common name, blue-green algae, although they are not usually scientifically classified as algae. They appear to have originated in a freshwater or terrestrial environment. Sericytochromatia, the proposed name of the paraphyletic and most basal group, is the ancestor of both the non-photosynthetic group Melainabacteria and the photosynthetic cyanobacteria, also called Oxyphotobacteria. Cyanobacteria use photosynthetic pigments, such as carotenoids, phycobilins, and various forms of chlorophyll, which absorb energy from light. Unlike heterotrophic prokaryotes, cyanobacteria have internal membranes. These are flattened sacs called thylakoids where photosynthesis is performed. Phototrophic eukaryotes such as green plants perform photosynthesis in plast ...
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Archaea
Archaea ( ; singular archaeon ) is a domain of single-celled organisms. These microorganisms lack cell nuclei and are therefore prokaryotes. Archaea were initially classified as bacteria, receiving the name archaebacteria (in the Archaebacteria kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from the other two domains, Bacteria and Eukaryota. Archaea are further divided into multiple recognized phyla. Classification is difficult because most have not been isolated in a laboratory and have been detected only by their gene sequences in environmental samples. Archaea and bacteria are generally similar in size and shape, although a few archaea have very different shapes, such as the flat, square cells of ''Haloquadratum walsbyi''. Despite this morphological similarity to bacteria, archaea possess genes and several metabolic pathways that are more closely related to those of eukaryotes, notably for the enzymes involved ...
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Bacillus Macerans
''Bacillus'' (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum ''Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural ''Bacilli'' is the name of the class of bacteria to which this genus belongs. ''Bacillus'' species can be either obligate aerobes which are dependent on oxygen, or facultative anaerobes which can survive in the absence of oxygen. Cultured ''Bacillus'' species test positive for the enzyme catalase if oxygen has been used or is present. ''Bacillus'' can reduce themselves to oval endospores and can remain in this dormant state for years. The endospore of one species from Morocco is reported to have survived being heated to 420 °C. Endospore formation is usually triggered by a lack of nutrients: the bacterium divides within its cell wall, and one side then engulfs the other. They are not true spores (i.e., not an offspring). Endospore formation ...
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Klebsiella Pneumoniae
''Klebsiella pneumoniae'' is a Gram-negative, non-motile, encapsulated, lactose-fermenting, facultative anaerobic, rod-shaped bacterium. It appears as a mucoid lactose fermenter on MacConkey agar. Although found in the normal flora of the mouth, skin, and intestines, it can cause destructive changes to human and animal lungs if aspirated, specifically to the alveoli resulting in bloody, brownish or yellow colored jelly like sputum. In the clinical setting, it is the most significant member of the genus ''Klebsiella'' of the Enterobacteriaceae. ''K. oxytoca'' and ''K. rhinoscleromatis'' have also been demonstrated in human clinical specimens. In recent years, ''Klebsiella'' species have become important pathogens in nosocomial infections. It naturally occurs in the soil, and about 30% of strains can fix nitrogen in anaerobic conditions. As a free-living diazotroph, its nitrogen-fixation system has been much-studied, and is of agricultural interest, as ''K. pneumoniae'' has been ...
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Desulfovibrio
''Desulfovibrio'' is a genus of Gram-negative sulfate-reducing bacteria. ''Desulfovibrio'' species are commonly found in aquatic environments with high levels of organic material, as well as in water-logged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers. Like other sulfate-reducing bacteria, ''Desulfovibrio'' was long considered to be obligately anaerobic. This is not strictly correct: while growth may be limited, these bacteria can survive in O2-rich environments. These types of bacteria are known as aerotolerant. Some'' Desulfovibrio'' species have in recent years been shown to have bioremediation potential for toxic radionuclides such as uranium by a reductive bioaccumulation process. Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI) Unassigned species: * "''D. caledonien ...
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