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Charles C. Richardson
Charles Clifton Richardson (born May 7, 1935) is an American biochemist and professor at Harvard University. Richardson received his undergraduate education at Duke University, where he majored in medicine. He received his M.D. at Duke Medical School in 1960. Richardson works as a professor at Harvard Medical School, and he served as editor/associate editor of the ''Annual Review of Biochemistry'' from 1972 to 2003. Richardson received the American Chemical Society Award in Biological Chemistry in 1968, as well as numerous other accolades. Early life and education Charles Richardson was born on May 7, 1935, in Wilson, North Carolina. His father, Barney Clifton Richardson, was an accountant at a local automobile dealership. His mother, Elizabeth Barefoot, was a housewife. At 11 years old, Richardson and his family moved to Columbia, South Carolina. Richardson graduated from Dreher High School and received a full scholarship to Duke University in 1953. Without completing a bachel ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wilson, NC
Wilson is a city in and the county seat of Wilson County, North Carolina, United States. Located approximately east of the capital city of Raleigh, North Carolina, Raleigh, it is served by the interchange of Interstate 95 in North Carolina, Interstate 95 and U.S. Route 264. Wilson had an estimated population of 49,459 in 2019, according to the U.S. Census Bureau, and is also an anchor city of the Rocky Mount-Wilson-Roanoke Rapids CSA, with a total population of 297,726 as of 2018. In the early 21st century, Wilson was ranked as 18th in size among North Carolina's 500-plus municipalities. From 1990 to 2010, the city population increased by more than 40 percent, primarily due to construction of new subdivisions that attracted many new residents. This has been accompanied by new retail and shopping construction, primarily in the northwestern parts of the city. Wilson is a diverse community; in 2012, the US Census estimated that 48% of the population identified as African American, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Duke Chapel
Duke University Chapel is a chapel located at the center of the campus of Duke University in Durham, North Carolina, Durham, North Carolina, United States. It is an ecumenism, ecumenical Christianity, Christian chapel and the center of religion at Duke, and has connections to the United Methodist Church. Constructed from 1930 to 1932, the Chapel seats about 1,800 people and stands 210 feet (64 m) tall, making it one of the tallest buildings in Durham County, North Carolina, Durham County. It is built in the Collegiate Gothic style, characterized by its large stones, pointed arches, and ribbed vaults. It has a 50-bell carillon and three pipe organs, one with 5,033 pipes and another with 6,900 pipes. The Chapel stands at the center of the university, on the highest ridge of Duke University's Duke University#West Campus, West Campus. Although plans for a chapel were first made in April 1925, the cornerstone was not laid until October 22, 1930. When it was completed in 1935 at a c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Ligase
DNA ligase is a specific type of enzyme, a ligase, () that facilitates the joining of DNA strands together by catalyzing the formation of a phosphodiester bond. It plays a role in repairing single-strand breaks in duplex DNA in living organisms, but some forms (such as DNA ligase IV) may specifically repair double-strand breaks (i.e. a break in both complementary strands of DNA). Single-strand breaks are repaired by DNA ligase using the complementary strand of the double helix as a template, with DNA ligase creating the final phosphodiester bond to fully repair the DNA. DNA ligase is used in both DNA repair and DNA replication (see '' Mammalian ligases''). In addition, DNA ligase has extensive use in molecular biology laboratories for recombinant DNA experiments (see '' Research applications''). Purified DNA ligase is used in gene cloning to join DNA molecules together to form recombinant DNA. Enzymatic mechanism The mechanism of DNA ligase is to form two covalent phos ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Exonuclease III
Exonuclease III (ExoIII) is an enzyme that belongs to the exonuclease family. ExoIII catalyzes the stepwise removal of mononucleotides from 3´-hydroxyl termini of double-stranded DNA. A limited number of nucleotides are removed during each binding event, resulting in coordinated progressive deletions within the population of DNA molecules. Function The preferred substrates are blunt or recessed 3´-termini, although ExoIII also acts at nicks in duplex DNA to produce single-strand gaps. The enzyme is not active on single-stranded DNA, and thus 3´-protruding termini are resistant to cleavage. The degree of resistance depends on the length of the extension, with extensions 4 bases or longer being essentially resistant to cleavage. This property is used to produce unidirectional deletions from a linear molecule with one resistant (3´-overhang) and one susceptible (blunt or 5´-overhang) terminus. ExoIII activity depends partially on the DNA helical structure and displays sequen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Replication
In molecular biology, DNA replication is the biological process of producing two identical replicas of DNA from one original DNA molecule. DNA replication occurs in all living organisms acting as the most essential part for biological inheritance. This is essential for cell division during growth and repair of damaged tissues, while it also ensures that each of the new cells receives its own copy of the DNA. The cell possesses the distinctive property of division, which makes replication of DNA essential. DNA is made up of a double helix of two complementary strands. The double helix describes the appearance of a double-stranded DNA which is thus composed of two linear strands that run opposite to each other and twist together to form. During replication, these strands are separated. Each strand of the original DNA molecule then serves as a template for the production of its counterpart, a process referred to as semiconservative replication. As a result of semi-conservative rep ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacteriophage
A bacteriophage (), also known informally as a ''phage'' (), is a duplodnaviria virus that infects and replicates within bacteria and archaea. The term was derived from "bacteria" and the Greek φαγεῖν ('), meaning "to devour". Bacteriophages are composed of proteins that encapsulate a DNA or RNA genome, and may have structures that are either simple or elaborate. Their genomes may encode as few as four genes (e.g. MS2) and as many as hundreds of genes. Phages replicate within the bacterium following the injection of their genome into its cytoplasm. Bacteriophages are among the most common and diverse entities in the biosphere. Bacteriophages are ubiquitous viruses, found wherever bacteria exist. It is estimated there are more than 1031 bacteriophages on the planet, more than every other organism on Earth, including bacteria, combined. Viruses are the most abundant biological entity in the water column of the world's oceans, and the second largest component of biom ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biochemistry
Biochemistry or biological chemistry is the study of chemical processes within and relating to living organisms. A sub-discipline of both chemistry and biology, biochemistry may be divided into three fields: structural biology, enzymology and metabolism. Over the last decades of the 20th century, biochemistry has become successful at explaining living processes through these three disciplines. Almost all areas of the life sciences are being uncovered and developed through biochemical methodology and research. Voet (2005), p. 3. Biochemistry focuses on understanding the chemical basis which allows biological molecules to give rise to the processes that occur within living cells and between cells,Karp (2009), p. 2. in turn relating greatly to the understanding of tissues and organs, as well as organism structure and function.Miller (2012). p. 62. Biochemistry is closely related to molecular biology, which is the study of the molecular mechanisms of biological phenomena.As ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tsuneko Okazaki
is a Japanese pioneer of molecular biology known for her work on DNA replication and specifically for discovering Okazaki fragments, along with her husband Reiji. Dr. Tsuneko Okazaki has continued to be involved in academia, contributing to more advancements in DNA research. Early life and education Tsuneko Okazaki was born in Nagoya, capital of the Aichi Prefecture of Japan, in 1933. She graduated from Aichi Prefectural Asahigaoka Senior High School. During her undergraduate years, she studied biology at Nagoya University School of Science. She graduated with her PhD from Nagoya University School of Science in 1956, which was also the year that she met her husband, Reiji Okazaki. They married that same year and soon after, they joined their research work and laboratories. Work leading to and discovery of Okazaki fragments Tsuneko and Reiji Okazaki's early research consisted of studying DNA synthesis and specific nucleotide characteristics in frog eggs and sea urchins. T ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Reiji Okazaki
was a pioneer Japanese molecular biologist, known for his research on DNA replication and especially for describing the role of Okazaki fragments along with his wife Tsuneko. Okazaki was born in Hiroshima, Japan. He graduated in 1953 from Nagoya University, and worked as a professor there after 1963. He died of leukemia in 1975 at the age of 44 while traveling to the United States; he had been heavily irradiated in Hiroshima when the first atomic bomb was dropped. Okazaki Fragments In 1968, Reiji and Tsuneko Okazaki discovered the way in which the lagging strand of DNA is replicated via fragments, now called Okazaki fragments. Their experiments used ''E. coli''. After introducing 3T-thymidine for only ten seconds to ''E. coli'' during DNA replication, they placed the sample in a test tube of alkaline sucrose. The larger, heavier DNA flowed to the bottom of the test tube, while the smaller, lighter DNA did not. When samples were taken from the bottom of the test tube, it was f ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Paul Berg
Paul Berg (born June 30, 1926) is an American biochemist and professor emeritus at Stanford University. He was the recipient of the Nobel Prize in Chemistry in 1980, along with Walter Gilbert and Frederick Sanger. The award recognized their contributions to basic research involving nucleic acids. Berg received his undergraduate education at Penn State University, where he majored in biochemistry. He received his Ph.D. in biochemistry from Case Western Reserve University in 1952. Berg worked as a professor at Washington University School of Medicine and Stanford University School of Medicine, in addition to serving as the director of the Beckman Center for Molecular and Genetic Medicine. In addition to the Nobel Prize, Berg was presented with the National Medal of Science in 1983 and the National Library of Medicine Medal in 1986. Berg is a member of the Board of Sponsors for the ''Bulletin of the Atomic Scientists''. Early life and education Berg was born in Brooklyn, New ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Escherichia Coli
''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus ''Escherichia'' that is commonly found in the lower intestine of warm-blooded organisms. Most ''E. coli'' strains are harmless, but some serotypes ( EPEC, ETEC etc.) can cause serious food poisoning in their hosts, and are occasionally responsible for food contamination incidents that prompt product recalls. Most strains do not cause disease in humans and are part of the normal microbiota of the gut; such strains are harmless or even beneficial to humans (although these strains tend to be less studied than the pathogenic ones). For example, some strains of ''E. coli'' benefit their hosts by producing vitamin K2 or by preventing the colonization of the intestine by pathogenic bacteria. These mutually beneficial relationships between ''E. col ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
DNA Polymerase
A DNA polymerase is a member of a family of enzymes that catalyze the synthesis of DNA molecules from nucleoside triphosphates, the molecular precursors of DNA. These enzymes are essential for DNA replication and usually work in groups to create two identical DNA duplexes from a single original DNA duplex. During this process, DNA polymerase "reads" the existing DNA strands to create two new strands that match the existing ones. These enzymes catalyze the chemical reaction : deoxynucleoside triphosphate + DNAn pyrophosphate + DNAn+1. DNA polymerase adds nucleotides to the three prime (3')-end of a DNA strand, one nucleotide at a time. Every time a cell divides, DNA polymerases are required to duplicate the cell's DNA, so that a copy of the original DNA molecule can be passed to each daughter cell. In this way, genetic information is passed down from generation to generation. Before replication can take place, an enzyme called helicase unwinds the DNA molecule from its tightl ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
