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Alicyclobacillus Acidocaldarius
''Alicyclobacillus acidocaldarius'' is a species of Gram positive, strictly aerobic, bacterium. The bacteria are acidophilic, thermophilic, and produce endospores. The first identified strains of ''A. acidocaldarius'' were from geysers in Yellowstone National Park and fumerole soil in Hawaii Volcano National Park. The species was originally classified as ''Bacillus acidocaldarius'' in 1971, but further 16S rRNA studies found that the species belonged in the newly created genus ''Alicyclobacillus''. The species name is derived from the Latin ''acidum'' (acid) and ''caldarius'' (pertaining to warm or hot), referring to the acidic and high temperature environments from which it was first isolated. Thomas D. Brock was one of the researchers who first categorized the species; his discovery of ''Thermus aquaticus'' allowed for other researchers to discover Taq polymerase and polyermase chain reaction (PCR). A strain of ''A. acidocaldarius'' has been isolated from thermally heated s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram Positive
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through an optical microscope. This is because the thick peptidoglycan layer in the bacterial cell wall retains the stain after it is washed away from the rest of the sample, in the decolorization stage of the test. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage degrades the outer membrane of gram-negative cells, making the cell wall more porous and incapable of retaining the crystal violet stain. Their peptidoglycan layer is much thinner and sandwiched between an inner cell membrane and a bacterial outer membrane, causing them to take up the counterstain (s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thermus Aquaticus
''Thermus aquaticus'' is a species of bacteria that can tolerate high temperatures, one of several thermophilic bacteria that belong to the ''Deinococcota'' phylum. It is the source of the heat-resistant enzyme ''Taq'' DNA polymerase, one of the most important enzymes in molecular biology because of its use in the polymerase chain reaction (PCR) DNA amplification technique. History When studies of biological organisms in hot springs began in the 1960s, scientists thought that the life of thermophilic bacteria could not be sustained in temperatures above about . Soon, however, it was discovered that many bacteria in different springs not only survived, but also thrived in higher temperatures. In 1969, Thomas D. Brock and Hudson Freeze of Indiana University reported a new species of thermophilic bacteria which they named ''Thermus aquaticus''. The bacterium was first isolated from Mushroom Spring in the Lower Geyser Basin of Yellowstone National Park, which is near the major Gr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gram-positive Bacteria
In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through an optical microscope. This is because the thick peptidoglycan layer in the bacterial cell wall retains the stain after it is washed away from the rest of the sample, in the decolorization stage of the test. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage degrades the outer membrane of gram-negative cells, making the cell wall more porous and incapable of retaining the crystal violet stain. Their peptidoglycan layer is much thinner and sandwiched between an inner cell membrane and a bacterial outer membrane, causing them to take up the counterstain (sa ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Alicyclobacillus Cycloheptanicus
''Alicyclobacillus cycloheptanicus'' is a species of Gram positive, strictly aerobic, bacterium. The bacteria are acidophilic and produce endospores. It was first isolated from soil. The species was originally classified as ''Bacillus cycloheptanicus'' in 1987, but further 16S rRNA studies found that the species belonged in the newly created genus ''Alicyclobacillus''. The species name refers ω-cycloheptane fatty acids in the cell membrane. ''A. cycloheptanicus'' was among the first three species reclassified from the genus ''Bacillus ''Bacillus'' (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum '' Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural ''Bacill ...'' to the newly created ''Alicyclobacillus'' in 1992, along with '' A. acidocaldarius'' and '' A. acidoterrestris''. The optimum growth temperature for ''A. cycloheptanicus'' is 48 °C, and can ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Alicyclobacillus Acidoterrestris
''Alicyclobacillus acidoterrestris'' is a species of Gram positive, strictly aerobic, bacterium. The bacteria are acidophilic and produce endospores. It was first isolated from soil. The species was originally classified as ''Bacillus acidoterrestris'' in 1987, but further 16S rRNA studies found that the species belonged in the newly created genus ''Alicyclobacillus''. The species name is derived from the Latin ''acidum'' (acid) and ''terrestris'' (from the earth), referring to its acidophilic nature and that it was isolated from soil. ''Alicyclobacillus acidoterrestris'' was among the first three species reclassified from the genus ''Bacillus ''Bacillus'' (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum '' Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural ''Bacill ...'' to the newly created ''Alicyclobacillus'' in 1992, along with '' A. acidocaldarius'' an ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacillus
''Bacillus'' (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum ''Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural ''Bacilli'' is the name of the class of bacteria to which this genus belongs. ''Bacillus'' species can be either obligate aerobes which are dependent on oxygen, or facultative anaerobes which can survive in the absence of oxygen. Cultured ''Bacillus'' species test positive for the enzyme catalase if oxygen has been used or is present. ''Bacillus'' can reduce themselves to oval endospores and can remain in this dormant state for years. The endospore of one species from Morocco is reported to have survived being heated to 420 °C. Endospore formation is usually triggered by a lack of nutrients: the bacterium divides within its cell wall, and one side then engulfs the other. They are not true spores (i.e., not an offspring). Endospore formation ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Polymerase Chain Reaction
The polymerase chain reaction (PCR) is a method widely used to rapidly make millions to billions of copies (complete or partial) of a specific DNA sample, allowing scientists to take a very small sample of DNA and amplify it (or a part of it) to a large enough amount to study in detail. PCR was invented in 1983 by the American biochemist Kary Mullis at Cetus Corporation; Mullis and biochemist Michael Smith (chemist), Michael Smith, who had developed other essential ways of manipulating DNA, were jointly awarded the Nobel Prize in Chemistry in 1993. PCR is fundamental to many of the procedures used in genetic testing and research, including analysis of Ancient DNA, ancient samples of DNA and identification of infectious agents. Using PCR, copies of very small amounts of DNA sequences are exponentially amplified in a series of cycles of temperature changes. PCR is now a common and often indispensable technique used in medical laboratory research for a broad variety of applications ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Taq Polymerase
''Taq'' polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism ''Thermus aquaticus,'' from which it was originally isolated by Chien et al. in 1976. Its name is often abbreviated to ''Taq'' or ''Taq'' pol. It is frequently used in the polymerase chain reaction (PCR), a method for greatly amplifying the quantity of short segments of DNA. ''T. aquaticus'' is a bacterium that lives in hot springs and hydrothermal vents, and ''Taq'' polymerase was identified as an enzyme able to withstand the protein-denaturing conditions (high temperature) required during PCR. Therefore, it replaced the DNA polymerase from '' E. coli'' originally used in PCR. Enzymatic properties ''Taqs optimum temperature for activity is 75–80 °C, with a half-life of greater than 2 hours at 92.5 °C, 40 minutes at 95 °C and 9 minutes at 97.5 °C, and can replicate a 1000 base pair strand of DNA in less than 10 seconds at 72 °C. At ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thomas D
Son Goku was a German rock band who played a mix of hardcore punk, alternative, reggae, and electronic sounds. The band was named after the Son Goku character in the ''Dragon Ball'' series. Son Goku's first and only album, ''Crashkurs'' (Crash course), was released in 2002 and reached a peak position of 15 on the Media Control Charts. The group has since disbanded due to "dissension" amongst the members. History Origins Son Goku was a side project of Thomas D of Die Fantastischen Vier. The band originated from the M.A.R.S. (Moderne Anstalt Rigoroser Spakker, or ''Modern Institute of Adamant Spakkers''), a commune of artists and musicians founded by Thomas D in 1998. Thomas D named the band after Son Goku, the protagonist of the ''Dragon Ball'' animated series, as the character embodied the band's philosophy; he stated he was "fascinated by Goku's naïveté and cheerfulness, yet, at the same time, a great warrior saving the world". Music Son Goku was formed with the "desire fo ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacterium
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationshi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Latin
Latin (, or , ) is a classical language belonging to the Italic branch of the Indo-European languages. Latin was originally a dialect spoken in the lower Tiber area (then known as Latium) around present-day Rome, but through the power of the Roman Republic it became the dominant language in the Italian region and subsequently throughout the Roman Empire. Even after the fall of Western Rome, Latin remained the common language of international communication, science, scholarship and academia in Europe until well into the 18th century, when other regional vernaculars (including its own descendants, the Romance languages) supplanted it in common academic and political usage, and it eventually became a dead language in the modern linguistic definition. Latin is a highly inflected language, with three distinct genders (masculine, feminine, and neuter), six or seven noun cases (nominative, accusative, genitive, dative, ablative, and vocative), five declensions, four verb conjuga ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
16S RRNA
16S rRNA may refer to: * 16S ribosomal RNA 16 S ribosomal RNA (or 16 S rRNA) is the RNA component of the 30S subunit of a prokaryotic ribosome ( SSU rRNA). It binds to the Shine-Dalgarno sequence and provides most of the SSU structure. The genes coding for it are referred to as 16S rR ..., the prokaryotic ribosomal subunit * Mitochondrially encoded 16S RNA, the eukaryotic ribosomal subunit {{Short pages monitor ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
