|
Ziehl–Neelsen Stain
Ziehl–Neelsen staining is a type of acid-fast stain, first introduced by Paul Ehrlich. Ziehl–Neelsen staining is a bacteriological stain used to identify acid-fast organisms, mainly Mycobacteria. It is named for two German doctors who modified the stain: the bacteriologist Franz Ziehl (1859–1926) and the pathologist Friedrich Neelsen (1854–1898). Mycobacteria In anatomic pathology specimens, immunohistochemistry and modifications of Ziehl–Neelsen staining (such as the Fite-Faraco method) have comparable diagnostic utility. Both of them are superior to traditional Ziehl–Neelsen staining. The genus ''Mycobacterium'' is a slow growing bacteria, made up of small rods that are slightly curved or straight, and are considered to be gram positive. Some mycobacteria are free-living saprophytes, but many are pathogens that cause disease in animals and humans. ''Mycobacterium bovis'' causes tuberculosis in cattle. Since tuberculosis can be spread to humans, milk is pasteurized ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mycobacterium Tuberculosis Ziehl-Neelsen Stain 02
''Mycobacterium'' is a genus of over 190 species in the phylum Actinomycetota, assigned its own family, Mycobacteriaceae. This genus includes pathogens known to cause serious diseases in mammals, including tuberculosis ('' M. tuberculosis'') and leprosy ('' M. leprae'') in humans. The Greek prefix ''myco-'' means 'fungus', alluding to this genus' mold-like colony surfaces. Since this genus has cell walls with Gram-positive and Gram-negative features, acid-fast staining is used to emphasize their resistance to acids, compared to other cell types. Metabolism and Morphology Mycobacteria are aerobic with 0.2-0.6 µm wide and 1.0-10 µm long rod shapes. They are generally non-motile, except for the species ''Mycobacterium marinum'', which has been shown to be motile within macrophages. Mycobacteria possess capsules and most do not form endospores. ''M. marinum'' and perhaps ''M. bovis'' have been shown to sporulate; however, this has been contested by further research. The disti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Histoplasmosis
Histoplasmosis is a fungal infection caused by ''Histoplasma capsulatum''. Symptoms of this infection vary greatly, but the disease affects primarily the lungs. Occasionally, other organs are affected; called disseminated histoplasmosis, it can be fatal if left untreated. Histoplasmosis is common among AIDS patients because of their suppressed immunity. In immunocompetent individuals, past infection results in partial protection against ill effects if reinfected. ''Histoplasma capsulatum'' is found in soil, often associated with decaying bat guano or bird droppings. Disruption of soil from excavation or construction can release infectious elements that are inhaled and settle into the lung. From 1938 to 2013 in the US, 105 outbreaks were reported in a total of 26 states plus Puerto Rico. In 1978 to 1979 during a large urban outbreak in which 100,000 people were exposed to the fungus in Indianapolis, victims had pericarditis, rheumatological syndromes, esophageal and vocal cord ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cell Wall
A cell wall is a structural layer surrounding some types of cells, just outside the cell membrane. It can be tough, flexible, and sometimes rigid. It provides the cell with both structural support and protection, and also acts as a filtering mechanism. Cell walls are absent in many eukaryotes, including animals, but are present in some other ones like fungi, algae and plants, and in most prokaryotes (except mollicute bacteria). A major function is to act as pressure vessels, preventing over-expansion of the cell when water enters. The composition of cell walls varies between taxonomic group and species and may depend on cell type and developmental stage. The primary cell wall of land plants is composed of the polysaccharides cellulose, hemicelluloses and pectin. Often, other polymers such as lignin, suberin or cutin are anchored to or embedded in plant cell walls. Algae possess cell walls made of glycoproteins and polysaccharides such as carrageenan and agar that are absent ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Malachite Green
Malachite green is an organic compound that is used as a dyestuff and controversially as an antimicrobial in aquaculture. Malachite green is traditionally used as a dye for materials such as silk, leather, and paper. Despite its name the dye is not prepared from the mineral malachite; the name just comes from the similarity of color. Structures and properties Malachite green is classified in the dyestuff industry as a triarylmethane dye and also using in pigment industry. Formally, malachite green refers to the chloride salt 6H5C(C6H4N(CH3)2)2l, although the term malachite green is used loosely and often just refers to the colored cation. The oxalate salt is also marketed. The anions have no effect on the color. The intense green color of the cation results from a strong absorption band at 621 nm ( extinction coefficient of ). Malachite green is prepared by the condensation of benzaldehyde and dimethylaniline to give leuco malachite green (LMG): :C6H5CHO + C6H5N(CH3)2 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methylene Blue
Methylthioninium chloride, commonly called methylene blue, is a salt used as a dye and as a medication. Methylene blue is a thiazine dye. As a medication, it is mainly used to treat methemoglobinemia by converting the ferric iron in hemoglobin to ferrous iron. Specifically, it is used to treat methemoglobin levels that are greater than 30% or in which there are symptoms despite oxygen therapy. It has previously been used for treating cyanide poisoning and urinary tract infections, but this use is no longer recommended. Methylene blue is typically given by injection into a vein. Common side effects include headache, vomiting, confusion, shortness of breath, and high blood pressure. Other side effects include serotonin syndrome, red blood cell breakdown, and allergic reactions. Use often turns the urine, sweat, and stool blue to green in color. While use during pregnancy may harm the baby, not using it in methemoglobinemia is likely more dangerous. Methylene blue was first pr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Carbol Fuchsin
Carbol fuchsin, carbol-fuchsin, carbolfuchsin, or Castellani's paint ( CAS ) is a mixture of phenol and basic fuchsin that is used in bacterial staining procedures. It is commonly used in the staining of mycobacteria because it has an affinity for the mycolic acids found in their cell membranes. It is a component of Ziehl–Neelsen stain, a differential stain. Carbol fuchsin is used as the primary stain dye to detect acid-fast bacteria because it is more soluble in the cells' wall lipids than in the acid alcohol. If the bacteria is acid-fast the bacteria will retain the initial red color of the dye because they are able to resist the destaining by acid alcohol (0.4–1% HCl in 70% EtOH). Additionally, it can be used for the staining of bacterial spores. Carbol-fuchsin is also used as a topical antiseptic An antiseptic (from Greek ἀντί ''anti'', "against" and σηπτικός ''sēptikos'', "putrefactive") is an antimicrobial substance or compound that is applied to livi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Joseph Kinyoun
Joseph James Kinyoun (November 25, 1860 – February 14, 1919) was an American physician and the founder of the United States' Hygienic Laboratory, the predecessor of the National Institutes of Health. Biography Early life Joseph James "Joe" Kinyoun was born November 25, 1860, in East Bend, North Carolina, the oldest of five children born to Elizabeth Ann Conrad and John Hendricks Kinyoun. His family settled in Post Oak, Missouri in 1866 after his house burned down during the Civil War. At the age of 16, he studied medicine with his father, John Hendricks Kinyoun, who was a general practitioner. His family joined a Baptist church. Kinyoun was educated at St. Louis Medical College and graduated from Bellevue Medical College in 1882 with an M.D. degree. He did postdoctoral studies in pathology and bacteriology at the Carnegie Laboratory where he became the first bacteriology student and studied cholera. Then he was a visiting scientist in Europe under Robert Koch. He was aw ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Robert Koch
Heinrich Hermann Robert Koch ( , ; 11 December 1843 – 27 May 1910) was a German physician and microbiologist. As the discoverer of the specific causative agents of deadly infectious diseases including tuberculosis, cholera (though the Vibrio cholerae, bacterium itself was discovered by Filippo Pacini in 1854), and anthrax, he is regarded as one of the main founders of modern bacteriology. As such he is popularly nicknamed the father of microbiology (with Louis Pasteur), and as the father of medical bacteriology. His discovery of the anthrax bacterium (''Bacillus anthracis'') in 1876 is considered as the birth of modern bacteriology. His discoveries directly provided proofs for the germ theory of diseases, and the scientific basis of public health. While working as a private physician, Koch developed many innovative techniques in microbiology. He was the first to use the oil immersion lens, Condenser (optics), condenser, and microphotography in microscopy. His invention of the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Paragonimiasis
Paragonimiasis is a food-borne parasitic disease caused by several species of lung flukes belonging to genus ''Paragonimus''. Infection is acquired by eating crustaceans such as crabs and crayfishes which host the infective forms called metacercariae, or by eating raw or undercooked meat of mammals harboring the metacercariae from crustaceans. More than 40 species of ''Paragonimus'' have been identified; 10 of these are known to cause disease in humans. The most common cause of human paragonimiasis is '' P. westermani'', the oriental lung fluke. About 22 million people are estimated to be affected yearly worldwide. It is particularly common in East Asia. Paragonimiasis is easily mistaken for other diseases with which it shares clinical symptoms, such as tuberculosis and lung cancer. Life cycle Not all ''Paragonimus'' species infect humans. However, all of them target mammals as their final (definitive) hosts. In mammalian lung tissue, the adult flukes live as encapsulated pairs. ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Isospora
''Isospora'' is a genus of internal parasites in the subclass Coccidia. It is responsible for the condition isosporiasis, which causes acute, non-bloody diarrhoea in immunocompromised individuals. Taxonomy At least 248 species were originally described in this genus. For instance, the house sparrow has 12 species of ''Isospora''. However, most species are little studied, and some authors doubt whether all should be recognized as distinct species. In 2005, all former ''Isospora'' species that infect mammalian hosts were reclassified as members of the genus ''Cystoisospora'', a member of the Sarcocystidae The Sarcocystidae are a family of Apicomplexa associated with a variety of diseases in humans and other animals. Taxonomy Genera in this family include: * ''Besnoitia'' * '' Cystoisospora'' * '' Frenkelia'' * '' Nephroisospora'' * ''Neospora'' ... family. References External links * Apicomplexa genera Conoidasida {{Apicomplexa-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cryptosporidium
''Cryptosporidium'', sometimes informally called crypto, is a genus of apicomplexan parasitic alveolates that can cause a respiratory and gastrointestinal illness (cryptosporidiosis) that primarily involves watery diarrhea (intestinal cryptosporidiosis), sometimes with a persistent cough (respiratory cryptosporidiosis). Treatment of gastrointestinal infection in humans involves fluid rehydration, electrolyte replacement, and management of any pain. , nitazoxanide is the only drug approved for the treatment of cryptosporidiosis in immunocompetent hosts. Supplemental zinc may improve symptoms, particularly in recurrent or persistent infections or in others at risk for zinc deficiency. ''Cryptosporidium'' oocysts are 4–6 μm in diameter and exhibit partial acid-fast staining. They must be differentiated from other partially acid-fast organisms including ''Cyclospora cayetanensis''. General characteristics ''Cryptosporidium'' causes cryptosporidiosis, an infection tha ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Russula
''Russula'' is a very large genus composed of around 750 worldwide species of ectomycorrhizal mushrooms. They are typically common, fairly large, and brightly colored – making them one of the most recognizable genera among mycologists and mushroom collectors. Their distinguishing characteristics include usually brightly coloured caps, a white to dark yellow spore print, brittle, attached gill (mushroom), gills, an absence of latex, and absence of partial veil or universal veil, volva tissue on the stem. Microscopically, the genus is characterised by the amyloid ornamented spores and flesh (trama) composed of spherocysts. Members of the related genus ''Lactarius (fungus), Lactarius'' have similar characteristics but emit a milky latex when their gills are broken. The genus was described by Christian Hendrik Persoon in 1796. Taxonomy Christian Hendrik Persoon first circumscription (taxonomy), circumscribed the genus ''Russula'' in his 1796 work ''Observationes Mycologicae'', and c ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
.jpg "Click for more on -> Joseph Kinyoun")
