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RAD-seq
Restriction site associated DNA (RAD) markers are a type of genetic marker which are useful for association mapping, Quantitative trait locus, QTL-mapping, population genetics, ecological genetics and evolutionary genetics. The use of RAD markers for genetic mapping is often called RAD mapping. An important aspect of RAD markers and mapping is the process of isolating RAD tags, which are the DNA sequences that immediately flank each instance of a particular restriction site of a restriction enzyme throughout the genome. Once RAD tags have been isolated, they can be used to identify and genotype DNA sequence polymorphisms mainly in form of Single-nucleotide polymorphism, single nucleotide polymorphisms (SNPs). Polymorphisms that are identified and genotyped by isolating and analyzing RAD tags are referred to as RAD markers. Although genotyping by sequencing presents an approach similar to the RAD-seq method, they differ in some substantial ways. Isolation of RAD tags The use of the ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Genotyping By Sequencing
In the field of DNA sequencing, genetic sequencing, genotyping by sequencing, also called GBS, is a method to discover Single-nucleotide polymorphism, single nucleotide polymorphisms (SNP) in order to perform genotyping studies, such as genome-wide association studies (Genome-wide association study, GWAS). GBS uses restriction enzymes to reduce genome complexity and genotype multiple DNA samples. After digestion, Polymerase chain reaction, PCR is performed to increase fragments pool and then GBS libraries are sequenced using next generation sequencing technologies, usually resulting in about 100bp single-end reads. It is relatively inexpensive and has been used in plant breeding. Although GBS presents an approach similar to Restriction site associated DNA markers, restriction-site-associated DNA sequencing (RAD-seq) method, they differ in some substantial ways. Methods GBS is a robust, simple, and affordable procedure for SNP discovery and mapping. Overall, this approach reduces ge ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |