Nitrospira Inopinata
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Nitrospira Inopinata
''Nitrospira inopinata'' is a bacterium from the phylum Nitrospirota. This phylum contains nitrite-oxidizing bacteria playing role in nitrification. However ''N. inopinata'' was shown to perform complete ammonia oxidation to nitrate thus being the first comammox bacterium to be discovered. ''N. inopinata'' was cultivated in enrichment culture. Initial inoculum was obtained in 2011 from microbial biofilm growing on metal surface of the pipe covered by hot water (56 °C, pH 7.5), which was raised from 1 200m deep oil exploration well. The well was located in Aushiger, North Caucasus, Russia. The growth in pure culture was achieved in 2017. Genome of ''N. inopinata'' was released in 2015 represented by 3.3 Mbp, with 3 116 genes and 59.2% GC content. NCBI The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH). It is approved and funded by the government of t ...
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Nitrospirota
Nitrospirota is a phylum of bacteria. It includes multiple genera, such as ''Nitrospira'', the largest. The first member of this phylum, ''Nitrospira marina'', was discovered in 1985. The second member, ''Nitrospira moscoviensis'', was discovered in 1995. Nitrospirota contains nitrifying taxa which oxidize nitrite to nitrate (nitrite-oxidizing bacteria, NOB) and commamox bacteria ''Nitrospira inopinata'' discovered in 2015 and cultivated in 2017. Phylogeny Taxonomy The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LSPN) and the National Center for Biotechnology Information (NCBI). * Phylum "Nitrospirota" Garrity & Holt 2021 ** Class " Leptospirillia" *** Order " Leptospirillales" **** Family " Leptospirillaceae" Cavalier-Smith 2020 ***** Genus '' Leptospirillum'' Markosyan 1972 ex Hippe 2000 ****** Species "'' L. ferrodiazotrophum''" Tyson et al. 2005 ****** Species '' L. ferriphilum'' Coram & Rawlings 2002 ****** Speci ...
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Nitrification
''Nitrification'' is the biological oxidation of ammonia to nitrite followed by the oxidation of the nitrite to nitrate occurring through separate organisms or direct ammonia oxidation to nitrate in comammox bacteria. The transformation of ammonia to nitrite is usually the rate limiting step of nitrification. Nitrification is an important step in the nitrogen cycle in soil. Nitrification is an aerobic process performed by small groups of autotrophic bacteria and archaea. Microbiology Ammonia oxidation The oxidation of ammonia into nitrite (also known as nitritation) is performed by two groups of organisms, ammonia-oxidizing bacteria (AOB) and ammonia-oxidizing archaea (AOA). AOB AOB can be found among the Betaproteobacteria and Gammaproteobacteria. Since discovery of AOA in 2005, two isolates have been cultivated: ''Nitrosopumilus maritimus'' and ''Nitrososphaera viennensis''. In soils the most studied AOB belong to the genera ''Nitrosomonas'' and '' Nitrococcus''. AOA ...
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Comammox
Comammox (COMplete AMMonia OXidation) is the name attributed to an organism that can convert ammonia into nitrite and then into nitrate through the process of nitrification. Nitrification has traditionally thought to be a two-step process, where ammonia-oxidizing bacteria and archaea oxidize ammonia to nitrite and then nitrite-oxidizing bacteria convert to nitrate. Complete conversion of ammonia into nitrate by a single microorganism was first predicted in 2006. In 2015 the presence of microorganisms that could carry out both conversion processes was discovered within the genus ''Nitrospira,'' and the nitrogen cycle was updated. Within the genus ''Nitrospira,'' the major ecosystems comammox are primarily found in natural aquifers and engineered ecosystems. Complete nitrification step yield more energy (∆G°′ = −349 kJ mol−1 NH3) than either single oxidation alone (∆G°′ = −275 kJ mol−1 NH3 for ammonia oxidation to nitrite and ∆G°′ = −74 kJ ...
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North Caucasus
The North Caucasus, ( ady, Темыр Къафкъас, Temır Qafqas; kbd, Ишхъэрэ Къаукъаз, İṩxhərə Qauqaz; ce, Къилбаседа Кавказ, Q̇ilbaseda Kavkaz; , os, Цӕгат Кавказ, Cægat Kavkaz, inh, Даькъасте, Däq̇aste, krc, Шимал Кавказ, Şimal Kavkaz, russian: Северный Кавказ, r=Severnyy Kavkaz, p=ˈsʲevʲɪrnɨj kɐfˈkas) or Ciscaucasia (russian: Предкавказье, Predkavkazye), is a subregion of Eastern Europe in the Eurasian continent. It is the northern part of the wider Caucasus region, and is entirely a part of Russia, sandwiched between the Sea of Azov and Black Sea to the west, and the Caspian Sea to the east. The region shares land borders with Georgia (country), Georgia and Azerbaijan to the south. Krasnodar is the largest city within the North Caucasus. Politically, the North Caucasus is made up of Russian Republics of Russia, republics and krais. It lies north of the Main C ...
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National Center For Biotechnology Information
The National Center for Biotechnology Information (NCBI) is part of the United States National Library of Medicine (NLM), a branch of the National Institutes of Health (NIH). It is approved and funded by the government of the United States. The NCBI is located in Bethesda, Maryland, and was founded in 1988 through legislation sponsored by US Congressman Claude Pepper. The NCBI houses a series of databases relevant to biotechnology and biomedicine and is an important resource for bioinformatics tools and services. Major databases include GenBank for DNA sequences and PubMed, a bibliographic database for biomedical literature. Other databases include the NCBI Epigenomics database. All these databases are available online through the Entrez search engine. NCBI was directed by David Lipman, one of the original authors of the BLAST sequence alignment program and a widely respected figure in bioinformatics. GenBank NCBI had responsibility for making available the GenBank DNA seque ...
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Nitrogen Cycle
The nitrogen cycle is the biogeochemical cycle by which nitrogen is converted into multiple chemical forms as it circulates among atmospheric, terrestrial, and marine ecosystems. The conversion of nitrogen can be carried out through both biological and physical processes. Important processes in the nitrogen cycle include fixation, ammonification, nitrification, and denitrification. The majority of Earth's atmosphere (78%) is atmospheric nitrogen, making it the largest source of nitrogen. However, atmospheric nitrogen has limited availability for biological use, leading to a scarcity of usable nitrogen in many types of ecosystems. The nitrogen cycle is of particular interest to ecologists because nitrogen availability can affect the rate of key ecosystem processes, including primary production and decomposition. Human activities such as fossil fuel combustion, use of artificial nitrogen fertilizers, and release of nitrogen in wastewater have dramatically altered the global nitr ...
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