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Microbial Contamination Of Diesel Fuel
Diesel bug is contamination of diesel fuel by microbes such as bacteria and fungi. Water can get into diesel fuel as a result of condensation, rainwater penetration or adsorption from the air — modern biodiesel is especially hygroscopic. The presence of water then encourages microbial growth which either occurs at the interface between the oil and water or on the tank walls, depending on whether the microbes need oxygen. Species which may grow in this way include: * bacteria — ''Clostridium''; '' Desulfotomaculum''; '' Desulfovibrio''; ''Flavobacterium''; ''Acidovorax facilis''; ''Pseudomonas''; ''Sarcina'' * fungi — ''Aspergillus''; '' Candida keroseneae''; ''Fusarium ''Fusarium'' is a large genus of filamentous fungi, part of a group often referred to as hyphomycetes, widely distributed in soil and associated with plants. Most species are harmless saprobes, and are relatively abundant members of the soil ...''; '' Hormoconis resinae'' Fuel companies agree that ...
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Diesel Fuel
Diesel fuel , also called diesel oil, is any liquid fuel specifically designed for use in a diesel engine, a type of internal combustion engine in which fuel ignition takes place without a spark as a result of compression of the inlet air and then injection of fuel. Therefore, diesel fuel needs good compression ignition characteristics. The most common type of diesel fuel is a specific fractional distillate of petroleum fuel oil, but alternatives that are not derived from petroleum, such as biodiesel, biomass to liquid (BTL) or gas to liquid (GTL) diesel are increasingly being developed and adopted. To distinguish these types, petroleum-derived diesel is sometimes called petrodiesel in some academic circles. In many countries, diesel fuel is standardised. For example, in the European Union, the standard for diesel fuel is EN 590. Diesel fuel has many colloquial names; most commonly, it is simply referred to as ''diesel''. In the United Kingdom, diesel fuel for on-road us ...
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Flavobacterium
''Flavobacterium'' is a genus of Gram-negative, nonmotile and motile, rod-shaped bacteria that consists of 130 recognized species. Flavobacteria are found in soil and fresh water in a variety of environments. Several species are known to cause disease in freshwater fish. ''Flavobacterium psychrophilum'' causes the bacterial cold water disease on salmonids and the rainbow trout fry disease on rainbow trout. '' F. columnare'' causes the cotton-wool disease on freshwater fishes. '' F. branchiophilum'' causes the bacterial gill disease on trout. Another member of this genus, '' F. okeanokoites'' is the original source for the type IIs restriction endonuclease ''Fok''I, used in Zinc finger nucleases and TALENs. Nylon-eating bacteria are a strain of ''Flavobacterium'' that is capable of digesting certain by-products of nylon 6 manufacture. Species who are a part of the genus ''Flavobacterium'' are most likely found scattered along in nature. These microbes are mostly found in ...
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Fusarium
''Fusarium'' is a large genus of filamentous fungi, part of a group often referred to as hyphomycetes, widely distributed in soil and associated with plants. Most species are harmless saprobes, and are relatively abundant members of the soil microbial community. Some species produce mycotoxins in cereal crops that can affect human and animal health if they enter the food chain. The main toxins produced by these ''Fusarium'' species are fumonisins and trichothecenes. Despite most species apparently being harmless (some existing on the skin as commensal members of the skin flora), some ''Fusarium'' species and subspecific groups are among the most important fungal pathogens of plants and animals. The name of ''Fusarium'' comes from Latin ''fusus'', meaning a spindle. Taxonomy The taxonomy of the genus is complex. A number of different schemes have been used, and up to 1,000 species have been identified at times, with approaches varying between wide and narrow concepts ...
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Candida Keroseneae
''Candida keroseneae'' is a species of yeast in the genus '' Candida'', family Saccharomycetaceae. Described as new to science in 2011, it was isolated from aviation fuel. Taxonomy The type strain of this yeast (IMI 395605T) was isolated from aircraft fuel (kerosene) sampled from a European aircraft. Later analysis demonstrated that the isolated strains were able to grow in liquid media containing 50% Jet A-1 aviation fuel. Molecular analysis was performed using the ribosomal RNA gene sequences of internal transcribed spacer regions in addition to the D1/D2 domains of the 26S nuclear ribosomal RNA gene. The two isolated strains clustered within the '' Candida membranifaciens'' clade, with '' C. tumulicola'' as the most closely related species. The specific epithet ''keroseneae'' is New Latin for kerosene, the substrate of the new species. Description The yeast cells, after growth on glucose-peptone-yeast extract broth culture for three days at , are egg-shaped to elongated ...
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Aspergillus
'''' () is a genus consisting of several hundred mold species found in various climates worldwide. ''Aspergillus'' was first catalogued in 1729 by the Italian priest and biologist Pier Antonio Micheli. Viewing the fungi under a microscope, Micheli was reminded of the shape of an '' aspergillum'' (holy water sprinkler), from Latin ''spargere'' (to sprinkle), and named the genus accordingly. Aspergillum is an asexual spore-forming structure common to all ''Aspergillus'' species; around one-third of species are also known to have a sexual stage. While some species of ''Aspergillus'' are known to cause fungal infections, others are of commercial importance. Taxonomy Species ''Aspergillus'' consists of 837 species of fungi. Growth and distribution ''Aspergillus'' is defined as a group of conidial fungi—that is, fungi in an asexual state. Some of them, however, are known to have a teleomorph (sexual state) in the Ascomycota. With DNA evidence, all members of the genu ...
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Sarcina (genus)
''Sarcina'' is a genus of Gram-positive cocci bacteria in the family Clostridiaceae. A synthesizer of microbial cellulose, various members of the genus are human flora and may be found in the skin and large intestine. The genus takes its name from the Latin word "sarcina," meaning pack or bundle, after the cuboidal (2x2x2) cellular associations they form during division along three planes. The genus's type species is '' Sarcina ventriculi'', a variety found on the surface of cereal seeds, in soil, mud, and in the stomachs of humans, rabbits, and guinea pigs. Species * ''Sarcina aurantiaca'' * '' Sarcina maxima'' has been reclassified to '' Clostridium maximum'' * '' Sarcina ventriculi, also known as Clostridium ventriculi'' * '' Sarcina lutea'' has been reclassified to ''Micrococcus luteus'' * '' Sarcina troglodytae'' is a chimpanzee pathogen In biology, a pathogen ( el, πάθος, "suffering", "passion" and , "producer of") in the oldest and broadest sense, is any org ...
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Pseudomonas
''Pseudomonas'' is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae and containing 191 described species. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Their ease of culture ''in vitro'' and availability of an increasing number of ''Pseudomonas'' strain genome sequences has made the genus an excellent focus for scientific research; the best studied species include '' P. aeruginosa'' in its role as an opportunistic human pathogen, the plant pathogen '' P. syringae'', the soil bacterium '' P. putida'', and the plant growth-promoting '' P. fluorescens, P. lini, P. migulae'', and ''P. graminis''. Because of their widespread occurrence in water and plant seeds such as dicots, the pseudomonads were observed early in the history of microbiology. The generic name ''Pseudomonas'' created for these organisms was defined in rather vague terms by Walter Migula ...
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Acidovorax Facilis
''Acidovorax facilis'' is an aerobic, chemoorganotrophic bacterium used as a soil inoculant in agriculture and horticulture. Description Members of ''A. facilis'' are generally 1.0-5.0  μm long and 0.2-0.7 μm wide. Under a microscope, they appear as straight to slightly curved rods that occurs singly or in short chains. ''A. facilis'' are motile via a single flagellum at one end of the bacterium. They are negative by Gram stain and positive by the oxidase test. When grown on nutrient agar, they form unpigmented colonies. They grow in the presence of oxygen. Uses ''A. facilis'' has been used in agriculture and horticulture as a soil additive to improve plant growth. Additionally, a nitrilase enzyme from ''A. facilis'' has been engineered into '' E. coli'' for the commercial production of 3-hydroxyvaleric acid. History ''A. facilis'' was originally isolated from lawn soil in the United States by Albert Schatz and Carlton Bovell in 1950 and named ''Hydrogenomas ...
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Desulfovibrio
''Desulfovibrio'' is a genus of Gram-negative sulfate-reducing bacteria. ''Desulfovibrio'' species are commonly found in aquatic environments with high levels of organic material, as well as in water-logged soils, and form major community members of extreme oligotrophic habitats such as deep granitic fractured rock aquifers. Like other sulfate-reducing bacteria, ''Desulfovibrio'' was long considered to be obligately anaerobic. This is not strictly correct: while growth may be limited, these bacteria can survive in O2-rich environments. These types of bacteria are known as aerotolerant. Some'' Desulfovibrio'' species have in recent years been shown to have bioremediation potential for toxic radionuclides such as uranium by a reductive bioaccumulation process. Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI) Unassigned species: * "''D. caledoniens ...
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Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic re ...
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Desulfotomaculum
''Desulfotomaculum'' is a genus of Gram-positive, obligately anaerobic soil bacteria. A type of sulfate-reducing bacteria, ''Desulfotomaculum'' can cause food spoilage in poorly processed canned foods. Their presence can be identified by the release of hydrogen sulfide gas with its rotten egg smell when the can is first opened. They are endospore-forming bacteria. A new strain of ''Desulfotomaculum'', called '' Desulforudis audaxviator'', was discovered in 2005 during drilling 2.8 km deep in the Mponeng gold mine in South Africa. The strain, found in water which has been isolated for tens of millions of years, exists completely independent of photosynthesis. The bacteria uses radiolytically-produced hydrogen gas, which is generated in that environment by the energy released by radioisotopes, as well as sulfates which may be generated both by the energy released by radioisotopes as well as by other chemical reactions, to form hydrogen sulfide, which replaces the hydroge ...
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Clostridium
''Clostridium'' is a genus of anaerobic, Gram-positive bacteria. Species of ''Clostridium'' inhabit soils and the intestinal tract of animals, including humans. This genus includes several significant human pathogens, including the causative agents of botulism and tetanus. It also formerly included an important cause of diarrhea, '' Clostridioides difficile'', which was reclassified into the '' Clostridioides'' genus in 2016. History In the late 1700s, Germany experienced a number of outbreaks of an illness that seemed connected to eating certain sausages. In 1817, the German neurologist Justinus Kerner detected rod-shaped cells in his investigations into this so-called sausage poisoning. In 1897, the Belgian biology professor Emile van Ermengem published his finding of an endospore-forming organism he isolated from spoiled ham. Biologists classified van Ermengem's discovery along with other known gram-positive spore formers in the genus '' Bacillus''. This classificatio ...
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