Chthonomonas Calidirosea
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Chthonomonas Calidirosea
''Chthonomonas calidirosea'' is a Gram-negative bacterium and also the first representative of the new class Chthonomonadetes within the phylum Armatimonadota. The Armatimonadota were previously known as candidate phylum OP10. OP10 was composed solely of environmental 16S rRNA gene clone sequences prior to ''C. calidirosea's'' relative, ''Armatimonas rosea's'' discovery. It is now known that bacterial communities from geothermal environments, are generally constituted by, at least 5–10% of bacteria belonging to Armatimonadota. Discovery ''Chthonomonas calidirosea'' was originally isolated from geothermally heated soil at Hell's Gate, Tikitere, New Zealand. The soil was aseptically sampled at an approximate depth of 15 cm below the surface. The sampled soil had a pH of 4.3 and an ''in situ'' temperature of 55 °C. Relatives The environmental 16S rRNA gene sequences, belonging to the phylum ''Armatimonadota'' are currently sorted into six groups. Groups 2, 5, and 6 co ...
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Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationsh ...
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Armatimonadota
''Armatimonadota'' is a phylum of gram-negative bacteria. History ''Armatimonadota'' was originally described solely on the basis of environmental 16S rRNA gene clone sequences, and was temporarily titled candidate phylum OP10. However, in 2011 a bacterial strain belonging to the phylum was isolated from an aquatic plant in Japan. The species was named ''Armatimonas rosea'' and was the first member of its phylum, genus, and species. Members ''Armatimonas rosea'', an aerobic chemoheterotrophic bacterium, strain YO-36T, was isolated from rhizoplane of an aquatic plant (a reed, ''Phragmites australis'') inhabiting a freshwater lake in Japan. ''Chthonomonas calidirosea'', an aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, strain T49(T), was isolated from geothermally heated soil at Hell's Gate, Tikitere, New Zealand. Phylogeny Taxonomy The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature ...
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Gram-negative
Gram-negative bacteria are bacteria that do not retain the crystal violet stain used in the Gram staining method of bacterial differentiation. They are characterized by their cell envelopes, which are composed of a thin peptidoglycan cell wall sandwiched between an inner cytoplasmic cell membrane and a bacterial outer membrane. Gram-negative bacteria are found in virtually all environments on Earth that support life. The gram-negative bacteria include the model organism ''Escherichia coli'', as well as many pathogenic bacteria, such as ''Pseudomonas aeruginosa'', ''Chlamydia trachomatis'', and ''Yersinia pestis''. They are a significant medical challenge as their outer membrane protects them from many antibiotics (including penicillin), detergents that would normally damage the inner cell membrane, and lysozyme, an antimicrobial enzyme produced by animals that forms part of the innate immune system. Additionally, the outer leaflet of this membrane comprises a complex lipo ...
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Aseptic Sampling
Aseptic sampling is the process of aseptically withdrawing materials used in biopharmaceutical processes for analysis so as not contaminate or alter the sample or the source of the sample.ASTM E1287-89(1999) "Standard Practice for Aseptic Sampling of Biological Materials," ASTM International, West Conshohocken, PA, 1999, DOI: 10.1520/E1287-89R99, www.astm.org Aseptic samples are drawn throughout the entire biopharmaceutical process (cell culture/fermentation, buffer & media prep, purification, final fill and finishStockdale, D. Overview of Aseptic Fill/Finish Manufacturing. Am. Pharm. Rev. 2004.). Analysis of the sample includes sterility, cell count/cell viability, metabolites, gases, osmolality and more. Aseptic sampling techniques Biopharmaceutical drug manufacturers widely use aseptic sampling deviceto enhance aseptic technique Asepsis is the state of being free from disease-causing micro-organisms (such as pathogenic bacteria, viruses, pathogenic fungi, and parasites). T ...
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Armatimonas Rosea
''Armatimonas rosea'' is a Gram-negative bacterium and also the first species to be characterized within the phylum Armatimonadota. The Armatimonadota were previously known as candidate phylum OP10. OP10 was composed solely of environmental 16S rRNA gene clone sequences prior to ''A. rosea's'' discovery. Discovery ''A. rosea'' was originally isolated from the rhizoplane of the aquatic plant ''Phragmites australis'' inhabiting a freshwater lake in the Yamanashi prefecture in Japan. Cultivation and isolation were completed in a low nutrient medium, DTS. Relatives The environmental 16S rRNA gene sequences belonging to the phylum ''Armatimonadota'' are currently sorted into six groups. Groups 2, 5, and 6 consist solely of sequences. Group 1 contains ''Armatimonas rosea'', Group 3 contains ''Chthonomonadetes calidirosea'', and Group 4 contains ''Fimbriimonas ginsengisoli''. ''Chthonomonas calidirosea'' strain T49T, an aerobic, saccharolytic, obligately thermophilic, motile, non- ...
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Fimbriimonas Ginsengisoli
''Fimbriimonas ginsengisoli'' is a Gram-negative bacterium and the first representative of the class ''Fimbriimonadia'' within the phylum ''Armatimonadota''. The ''Armatimonadota'' were previously known as candidate phylum OP10. OP10 was composed solely of environmental 16S rRNA gene clone sequences prior to ''F. ginsengisoli's'' relative, ''Armatimonas rosea's'' discovery. Discovery ''F. ginsengisoli'' was originally isolated from a soil sample from a ginseng field in the Pocheon province, South Korea. Cultivation took place on one-half strength R2A agar. Relatives The environmental 16S rRNA gene sequences, belonging to the phylum ''Armatimonadota'' are currently sorted into six groups. Groups 2, 5, and 6 consist solely of sequences. Group 1 contains ''Armatimonas rosea'', Group 3 contains ''Chthonomonadetes calidirosea'', and Group 4 contains ''Fimbriimonas ginsengisoli''. ''Chthonomonas calidirosea'' strain T49T, an aerobic organism, aerobic, saccharolytic, obligately th ...
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Aerobic Organism
Aerobic means "requiring air," in which "air" usually means oxygen. Aerobic may also refer to * Aerobic exercise, prolonged exercise of moderate intensity * Aerobics, a form of aerobic exercise * Aerobic respiration, the aerobic process of cellular respiration * Aerobic organism Aerobic means "requiring air," in which "air" usually means oxygen. Aerobic may also refer to * Aerobic exercise, prolonged exercise of moderate intensity * Aerobics Aerobics is a form of physical exercise that combines rhythmic aerobic exe ..., a living thing with an oxygen-based metabolism See also * Anaerobic (other) {{disambiguation ...
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Armatimonadetes
''Armatimonadota'' is a phylum of gram-negative bacteria. History ''Armatimonadota'' was originally described solely on the basis of environmental 16S rRNA gene clone sequences, and was temporarily titled candidate phylum OP10. However, in 2011 a bacterial strain belonging to the phylum was isolated from an aquatic plant in Japan. The species was named '' Armatimonas rosea'' and was the first member of its phylum, genus, and species. Members '' Armatimonas rosea'', an aerobic chemoheterotrophic bacterium, strain YO-36T, was isolated from rhizoplane of an aquatic plant (a reed, ''Phragmites australis'') inhabiting a freshwater lake in Japan. ''Chthonomonas calidirosea'', an aerobic, saccharolytic, obligately thermophilic, motile, non-spore-forming bacterium, strain T49(T), was isolated from geothermally heated soil at Hell's Gate, Tikitere, New Zealand. Phylogeny Taxonomy The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomencla ...
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