The ''Pseudomon''-1 RNA motif is a conserved
RNA identified by
bioinformatics.
It is used by most
species
In biology, a species is the basic unit of classification and a taxonomic rank of an organism, as well as a unit of biodiversity. A species is often defined as the largest group of organisms in which any two individuals of the appropriate s ...
whose
genome
In the fields of molecular biology and genetics, a genome is all the genetic information of an organism. It consists of nucleotide sequences of DNA (or RNA in RNA viruses). The nuclear genome includes protein-coding genes and non-coding g ...
s have been sequenced and that are classified within the
genus
Genus ( plural genera ) is a taxonomic rank used in the biological classification of living and fossil organisms as well as viruses. In the hierarchy of biological classification, genus comes above species and below family. In binomial nom ...
''
Pseudomonas
''Pseudomonas'' is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae and containing 191 described species. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able t ...
'', and is also present in ''
Azotobacter vinelandii'', a closely related species. It is presumed to function as a
non-coding RNA
A non-coding RNA (ncRNA) is a functional RNA molecule that is not Translation (genetics), translated into a protein. The DNA sequence from which a functional non-coding RNA is transcribed is often called an RNA gene. Abundant and functionally im ...
. ''Pseudomon''-1 RNAs consistently have a downstream
rho-independent transcription terminator.
The intergenic region containing ''Pseudomon''-1 RNAs were also detected later by an independent study based on deep sequencing and called SPA0122
The genes surrounding this region suggest a similarity to
Spot 42 RNA,
but the ''Pseudomonas'' RNA functions to regulate the AlgC enzyme
Based on this information, the new name ErsA for the RNA was adopted.
Negatively regulates major porin ''oprD'' expression responsible for uptake of
carbapenem
Carbapenems are a class of very effective antibiotic agents most commonly used for the treatment of severe bacterial infections. This class of antibiotics is usually reserved for known or suspected multidrug-resistant (MDR) bacterial infections. ...
antibiotics, by base pairing with ''oprD''
5′UTR (leading to increased bacterial resistance to
meropenem
Meropenem, sold under the brand name Merrem among others, is an intravenous β-lactam antibiotic used to treat a variety of bacterial infections. Some of these include meningitis, intra-abdominal infection, pneumonia, sepsis, and anthrax.
...
). ErsA positively regulates amrZ mRNA and contributes to biofilm formation and motility.
References
External links
*
Non-coding RNA
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