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Nanosensors are nanoscale devices that measure physical quantities and convert these to signals that can be detected and analyzed. There are several ways proposed today to make nanosensors; these include top-down lithography, bottom-up assembly, and
molecular self-assembly In chemistry and materials science, molecular self-assembly is the process by which molecules adopt a defined arrangement without guidance or management from an outside source. There are two types of self-assembly: intramolecular and intermol ...
. There are different types of nanosensors in the market and in development for various applications, most notably in defense, environmental, and healthcare industries. These sensors share the same basic workflow: a selective binding of an analyte, signal generation from the interaction of the nanosensor with the bio-element, and processing of the signal into useful metrics.


Characteristics

Nanomaterials-based sensors have several benefits in
sensitivity and specificity ''Sensitivity'' and ''specificity'' mathematically describe the accuracy of a test which reports the presence or absence of a condition. Individuals for which the condition is satisfied are considered "positive" and those for which it is not are ...
over sensors made from traditional materials, due to nanomaterial features not present in bulk material that arise at the nanoscale. Nanosensors can have increased specificity because they operate at a similar scale as natural biological processes, allowing functionalization with chemical and biological molecules, with recognition events that cause detectable physical changes. Enhancements in sensitivity stem from the high surface-to-volume ratio of nanomaterials, as well as novel physical properties of nanomaterials that can be used as the basis for detection, including
nanophotonics Nanophotonics or nano-optics is the study of the behavior of light on the nanometer scale, and of the interaction of nanometer-scale objects with light. It is a branch of optics, optical engineering, electrical engineering, and nanotechnolog ...
. Nanosensors can also potentially be integrated with
nanoelectronics Nanoelectronics refers to the use of nanotechnology in electronic components. The term covers a diverse set of devices and materials, with the common characteristic that they are so small that inter-atomic interactions and quantum mechanical p ...
to add native processing capability to the nanosensor. In addition to their sensitivity and specificity, nanosensors offer significant advantages in cost and response times, making them suitable for high-throughput applications. Nanosensors provide real-time monitoring compared to traditional detection methods such as chromatography and spectroscopy. These traditional methods may take days to weeks to obtain results and often require investment in capital costs as well as time for sample preparation. One-dimensional nanomaterials such as
nanowire A nanowire is a nanostructure in the form of a wire with the diameter of the order of a nanometre (10−9 metres). More generally, nanowires can be defined as structures that have a thickness or diameter constrained to tens of nanometers or less ...
s and nanotubes are well suited for use in nanosensors, as compared to bulk or
thin-film A thin film is a layer of material ranging from fractions of a nanometer (monolayer) to several micrometers in thickness. The controlled synthesis of materials as thin films (a process referred to as deposition) is a fundamental step in many ap ...
planar devices. They can function both as transducers and wires to transmit the signal. Their high surface area can cause large signal changes upon binding of an analyte. Their small size can enable extensive
multiplexing In telecommunications and computer networking, multiplexing (sometimes contracted to muxing) is a method by which multiple analog or digital signals are combined into one signal over a shared medium. The aim is to share a scarce resource - ...
of individually addressable sensor units in a small device. Their operation is also "label free" in the sense of not requiring fluorescent or radioactive labels on the analytes. Zinc oxide nanowire is used for gas sensing applications, given that it exhibits high sensitivity toward low concentration of gas under ambient conditions and can be fabricated easily with low cost. There are several challenges for nanosensors, including avoiding drift and
fouling Fouling is the accumulation of unwanted material on solid surfaces. The fouling materials can consist of either living organisms (biofouling) or a non-living substance (inorganic or organic). Fouling is usually distinguished from other surf ...
, developing reproducible calibration methods, applying preconcentration and separation methods to attain a proper analyte concentration that avoids saturation, and integrating the nanosensor with other elements of a sensor package in a reliable manufacturable manner. Because nanosensors are a relatively new technology, there are many unanswered questions regarding nanotoxicology, which currently limits their application in biological systems. Potential applications for nanosensors include medicine, detection of contaminants and pathogens, and monitoring manufacturing processes and transportation systems. By measuring changes in physical properties (
volume Volume is a measure of occupied three-dimensional space. It is often quantified numerically using SI derived units (such as the cubic metre and litre) or by various imperial or US customary units (such as the gallon, quart, cubic inch). ...
,
concentration In chemistry, concentration is the abundance of a constituent divided by the total volume of a mixture. Several types of mathematical description can be distinguished: '' mass concentration'', '' molar concentration'', ''number concentration'', ...
, displacement and
velocity Velocity is the directional speed of an object in motion as an indication of its rate of change in position as observed from a particular frame of reference and as measured by a particular standard of time (e.g. northbound). Velocity i ...
,
gravitational In physics, gravity () is a fundamental interaction which causes mutual attraction between all things with mass or energy. Gravity is, by far, the weakest of the four fundamental interactions, approximately 1038 times weaker than the str ...
,
electrical Electricity is the set of physical phenomena associated with the presence and motion of matter that has a property of electric charge. Electricity is related to magnetism, both being part of the phenomenon of electromagnetism, as described ...
, and
magnetic Magnetism is the class of physical attributes that are mediated by a magnetic field, which refers to the capacity to induce attractive and repulsive phenomena in other entities. Electric currents and the magnetic moments of elementary particl ...
forces,
pressure Pressure (symbol: ''p'' or ''P'') is the force applied perpendicular to the surface of an object per unit area over which that force is distributed. Gauge pressure (also spelled ''gage'' pressure)The preferred spelling varies by country a ...
, or
temperature Temperature is a physical quantity that expresses quantitatively the perceptions of hotness and coldness. Temperature is measured with a thermometer. Thermometers are calibrated in various temperature scales that historically have relied o ...
) nanosensors may be able to distinguish between and recognize certain cells at the molecular level in order to deliver medicine or monitor development to specific places in the body. The type of signal transduction defines the major classification system for nanosensors. Some of the main types of nanosensor readouts include optical, mechanical, vibrational, or electromagnetic. As an example of classification, nanosensors that use
molecularly imprinted polymer A molecularly imprinted polymer (MIP) is a polymer that has been processed using the molecular imprinting technique which leaves cavities in the polymer matrix with an affinity for a chosen "template" molecule. The process usually involves initiati ...
s (MIP) can be divided into three categories, which are
electrochemical Electrochemistry is the branch of physical chemistry concerned with the relationship between electrical potential difference, as a measurable and quantitative phenomenon, and identifiable chemical change, with the potential difference as an outc ...
,
piezoelectric Piezoelectricity (, ) is the electric charge that accumulates in certain solid materials—such as crystals, certain ceramics, and biological matter such as bone, DNA, and various proteins—in response to applied mechanical stress. The word '' ...
, or
spectroscopic Spectroscopy is the field of study that measures and interprets the electromagnetic spectra that result from the interaction between electromagnetic radiation and matter as a function of the wavelength or frequency of the radiation. Matter wa ...
sensors.  Electrochemical sensors  induce a change in the electrochemical properties of the sensing material, which includes
charge Charge or charged may refer to: Arts, entertainment, and media Films * '' Charge, Zero Emissions/Maximum Speed'', a 2011 documentary Music * ''Charge'' (David Ford album) * ''Charge'' (Machel Montano album) * ''Charge!!'', an album by The Aqu ...
,
conductivity Conductivity may refer to: *Electrical conductivity, a measure of a material's ability to conduct an electric current **Conductivity (electrolytic), the electrical conductivity of an electrolyte in solution ** Ionic conductivity (solid state), ele ...
, and
electric potential The electric potential (also called the ''electric field potential'', potential drop, the electrostatic potential) is defined as the amount of work energy needed to move a unit of electric charge from a reference point to the specific point in ...
. Piezoelectric sensors either convert mechanical force into electric force or vice versa. This force is then transduced into a signal. MIP spectroscopic sensors can be divided into three subcategories, which are chemiluminescent sensors,
surface plasmon resonance Surface plasmon resonance (SPR) is the resonant oscillation of conduction electrons at the interface between negative and positive permittivity material in a particle stimulated by incident light. SPR is the basis of many standard tools for measu ...
sensors, and
fluorescence Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, tha ...
sensors. As the name suggests, these sensors produce light based signals in forms of chemiluminescence, resonance, and fluorescence. As described by the examples, the type of change that the sensor detects and type of signal it induces depend on the type of sensor


Mechanisms of operation

There are multiple mechanisms by which a recognition event can be transduced into a measurable signal; generally, these take advantage of the nanomaterial sensitivity and other unique properties to detect a selectively bound analyte. Electrochemical nanosensors are based on detecting a resistance change in the nanomaterial upon binding of an analyte, due to changes in
scattering Scattering is a term used in physics to describe a wide range of physical processes where moving particles or radiation of some form, such as light or sound, are forced to deviate from a straight trajectory by localized non-uniformities (including ...
or to the depletion or accumulation of
charge carrier In physics, a charge carrier is a particle or quasiparticle that is free to move, carrying an electric charge, especially the particles that carry electric charges in electrical conductors. Examples are electrons, ions and holes. The term i ...
s. One possibility is to use nanowires such as
carbon nanotubes A scanning tunneling microscopy image of a single-walled carbon nanotube Rotating single-walled zigzag carbon nanotube A carbon nanotube (CNT) is a tube made of carbon with diameters typically measured in nanometers. ''Single-wall carbon na ...
,
conductive polymer Conductive polymers or, more precisely, intrinsically conducting polymers (ICPs) are organic polymers that conduct electricity. Such compounds may have metallic conductivity or can be semiconductors. The biggest advantage of conductive polymers ...
s, or metal oxide nanowires as gates in
field-effect transistor The field-effect transistor (FET) is a type of transistor that uses an electric field to control the flow of current in a semiconductor. FETs (JFETs or MOSFETs) are devices with three terminals: ''source'', ''gate'', and ''drain''. FETs co ...
s, although as of 2009 they had not yet been demonstrated in real-world conditions. Chemical nanosensors contain a chemical recognition system (receptor) and a physiochemical transducer, in which the receptor interacts with analyte to produce electrical signals. In one case, upon interaction of the analyte with the receptor, the nanoporous transducer had a change in impedance which was determined as the sensor signal. Other examples include electromagnetic or
plasmonic In physics, a plasmon is a quantum of plasma oscillation. Just as light (an optical oscillation) consists of photons, the plasma oscillation consists of plasmons. The plasmon can be considered as a quasiparticle since it arises from the quantiz ...
nanosensors, spectroscopic nanosensors such as
surface-enhanced Raman spectroscopy Surface-enhanced Raman spectroscopy or surface-enhanced Raman scattering (SERS) is a surface-sensitive technique that enhances Raman scattering by molecules adsorbed on rough metal surfaces or by nanostructures such as plasmonic-magnetic silica ...
, magnetoelectronic or spintronic nanosensors, and mechanical nanosensors. Biological nanosensors consist of a bio-receptor and a transducer. The transduction method of choice is currently fluorescence because of the high sensitivity and relative ease of measurement. The measurement can be achieved by using the following methods: binding active nanoparticles to active proteins within the cell, using
site-directed mutagenesis Site-directed mutagenesis is a molecular biology method that is used to make specific and intentional mutating changes to the DNA sequence of a gene and any gene products. Also called site-specific mutagenesis or oligonucleotide-directed mutagenesi ...
to produce indicator proteins, allowing for real-time measurements, or by creating a nanomaterial (e.g. nanofibers) with attachment sites for the bio-receptors. Even though electrochemical nanosensors can be used to measure
intracellular This glossary of biology terms is a list of definitions of fundamental terms and concepts used in biology, the study of life and of living organisms. It is intended as introductory material for novices; for more specific and technical definitions ...
properties, they are typically less selective for biological measurements, as they lack the high specificity of bio-receptors (e.g. antibody, DNA).
Photonic Photonics is a branch of optics that involves the application of generation, detection, and manipulation of light in form of photons through emission, transmission, modulation, signal processing, switching, amplification, and sensing. Though ...
devices can also be used as nanosensors to quantify concentrations of clinically relevant samples. A principle of operation of these sensors is based on the chemical modulation of a hydrogel film volume that incorporates a Bragg grating. As the
hydrogel A hydrogel is a crosslinked hydrophilic polymer that does not dissolve in water. They are highly absorbent yet maintain well defined structures. These properties underpin several applications, especially in the biomedical area. Many hydrogels ar ...
swells or shrinks upon chemical stimulation, the Bragg grating changes color and diffracts light at different wavelengths. The diffracted light can be correlated with the concentration of a target analyte. Another type of nanosensor is one that works through a
colorimetric Colorimetry is "the science and technology used to quantify and describe physically the human color perception". It is similar to spectrophotometry, but is distinguished by its interest in reducing spectra to the physical correlates of color ...
basis. Here, the presence of the
analyte An analyte, component (in clinical chemistry), or chemical species is a substance or chemical constituent that is of interest in an analytical procedure. The purest substances are referred to as analytes, such as 24 karat gold, NaCl, water, etc. ...
causes a
chemical reaction A chemical reaction is a process that leads to the chemical transformation of one set of chemical substances to another. Classically, chemical reactions encompass changes that only involve the positions of electrons in the forming and breaking ...
or morphological alteration for a visible color change to occur. One such application, is that gold
nanoparticle A nanoparticle or ultrafine particle is usually defined as a particle of matter that is between 1 and 100 nanometres (nm) in diameter. The term is sometimes used for larger particles, up to 500 nm, or fibers and tubes that are less than 10 ...
s can be used for the detection of heavy metals. Many harmful gases can also be detected by a colorimetric change, such as through the commercially availabl
Dräger Tube
These provide an alternative to bulky, lab-scale systems, as these can be miniaturized to be used for point-of-sample devices. For example, many chemicals are regulated by the
Environmental Protection Agency A biophysical environment is a biotic and abiotic surrounding of an organism or population, and consequently includes the factors that have an influence in their survival, development, and evolution. A biophysical environment can vary in scale ...
and require extensive testing to ensure
contaminant Contamination is the presence of a constituent, impurity, or some other undesirable element that spoils, corrupts, infects, makes unfit, or makes inferior a material, physical body, natural environment, workplace, etc. Types of contamination Wi ...
levels are within the appropriate limits. Colorimetric nanosensors provide a method for on-site determination of many contaminants.


Production methods

The production method plays a central role in determining the characteristics of the manufactured nanosensor in that the function of nanosensor can be made through controlling the surface of nanoparticles. There are two main approaches in the manufacturing of nanosensors: top-down methods, which begin with a pattern generated at a larger scale, and then reduced to microscale. Bottom-up methods start with atoms or molecules that build up to nanostructures.


Top-down methods


Lithography

It involves starting out with a larger block of some material and carving out the desired form. These carved out devices, notably put to use in specific
microelectromechanical systems Microelectromechanical systems (MEMS), also written as micro-electro-mechanical systems (or microelectronic and microelectromechanical systems) and the related micromechatronics and microsystems constitute the technology of microscopic devices, ...
used as microsensors, generally only reach the
micro Micro may refer to: Measurement * micro- (μ), a metric prefix denoting a factor of 10−6 Places * Micro, North Carolina, town in U.S. People * DJ Micro, (born Michael Marsicano) an American trance DJ and producer * Chii Tomiya (都宮 ち ...
size, but the most recent of these have begun to incorporate nanosized components. One of the most common method is called electron beam lithography. Although very costly, this technique effectively forms a distribution of circular or ellipsoidal plots on the two dimensional surface. Another method is electrodeposition, which requires conductive elements to produce miniaturized devices.Pison, U., Giersig, M., & Schaefer, Alex. (2014). US 8846580 B2. Berlin, Germany.


Fiber pulling

This method consists in using a tension device to stretch the major axis of a fiber while it is heated, to achieve nano-sized scales. This method is specially used in optical fiber to develop optical-fiber-based nanosensors.


Chemical etching

Two different types of chemical etching have been reported. In th
Turner method
a fiber is etched to a point while placed in the meniscus between
hydrofluoric acid Hydrofluoric acid is a solution of hydrogen fluoride (HF) in water. Solutions of HF are colourless, acidic and highly corrosive. It is used to make most fluorine-containing compounds; examples include the commonly used pharmaceutical antidepres ...
and an organic overlayer. This technique has been shown to produce fibers with large taper angles (thus increasing the light reaching the tip of the fiber) and tip diameters comparable to the pulling method. The second method is tube etching, which involves etching an optical fiber with a single-component solution of
hydrogen fluoride Hydrogen fluoride (fluorane) is an inorganic compound with the chemical formula . This colorless gas or liquid is the principal industrial source of fluorine, often as an aqueous solution called hydrofluoric acid. It is an important feedstock ...
. A silica fiber, surrounded with an organic cladding, is polished and one end is placed in a container of hydrofluoric acid. The acid then begins to etch away the tip of the fiber without destroying the cladding. As the silica fiber is etched away, the polymer cladding acts as a wall, creating microcurrents in the hydrofluoric acid that, coupled with
capillary action Capillary action (sometimes called capillarity, capillary motion, capillary rise, capillary effect, or wicking) is the process of a liquid flowing in a narrow space without the assistance of, or even in opposition to, any external forces li ...
, cause the fiber to be etched into the shape of a cone with large, smooth tapers. This method shows much less susceptibility to environmental parameters than the Turner method.


Bottom-up methods

This type of methods involve assembling the sensors out of smaller components, usually individual
atoms Every atom is composed of a nucleus and one or more electrons bound to the nucleus. The nucleus is made of one or more protons and a number of neutrons. Only the most common variety of hydrogen has no neutrons. Every solid, liquid, gas ...
or molecules. This is done by arranging atoms in specific patterns, which has been achieved in laboratory tests through use of
atomic force microscopy Atomic force microscopy (AFM) or scanning force microscopy (SFM) is a very-high-resolution type of scanning probe microscopy (SPM), with demonstrated resolution on the order of fractions of a nanometer, more than 1000 times better than the opt ...
, but is still difficult to achieve
en masse Many words in the English vocabulary are of French origin, most coming from the Anglo-Norman spoken by the upper classes in England for several hundred years after the Norman Conquest, before the language settled into what became Modern Engli ...
and is not economically viable.


Self-assembly

Also known as “growing”, this method most often entails an already complete set of components that would automatically assemble themselves into a finished product. Accurately being able to reproduce this effect for a desired sensor in a laboratory would imply that scientists could manufacture nanosensors much more quickly and potentially far more cheaply by letting numerous molecules assemble themselves with little or no outside influence, rather than having to manually assemble each sensor. Although the conventional fabrication techniques have proven to be efficient, further improvements in the production method can lead to minimization of cost and enhancement in performance. Challenges with current production methods include uneven distribution, size, and shape of nanoparticles, which all lead to limitation in performance. In 2006, researchers in Berlin patented their invention of a novel diagnostic nanosensor fabricated with nanosphere lithography (NSL), which allows precise control over size and shape of nanoparticles and creates nanoislands. The metallic nanoislands produced an increase in signal transduction and thus increased sensitivity of the sensor. The results also showed that the sensitivity and specification of the diagnostic nanosensor depend on the size of the nanoparticles, that decreasing the nanoparticle size increases the sensitivity.


Applications

One of the first working examples of a synthetic nanosensor was built by researchers at the
Georgia Institute of Technology The Georgia Institute of Technology, commonly referred to as Georgia Tech or, in the state of Georgia, as Tech or The Institute, is a public research university and institute of technology in Atlanta, Georgia. Established in 1885, it is part ...
in 1999. It involved attaching a single particle onto the end of a
carbon nanotube A scanning tunneling microscopy image of a single-walled carbon nanotube Rotating single-walled zigzag carbon nanotube A carbon nanotube (CNT) is a tube made of carbon with diameters typically measured in nanometers. ''Single-wall carbon na ...
and measuring the
vibrational frequency A molecular vibration is a periodic motion of the atoms of a molecule relative to each other, such that the center of mass of the molecule remains unchanged. The typical vibrational frequencies range from less than 1013 Hz to approximately 1014 ...
of the nanotube both with and without the particle. The discrepancy between the two frequencies allowed the researchers to measure the mass of the attached particle. Since then, increasing amounts of research have gone into nanosensors, whereby modern nanosensors have been developed for many applications.  Currently, the applications of nanosensors in the market include: healthcare, defense and military, and others such as food, environment, and agriculture.Technavio.  Investment in the Global Nanosensors Market. 2017.


Defense and military

Nanoscience as a whole has many potential applications in the defense and military sector- including chemical detection, decontamination, and forensics. Some nanosensors in development for defense applications include nanosensors for the detection of explosives or toxic gases. Such nanosensors work on the principle that gas molecules can be distinguished based on their mass using, for example, piezoelectric sensors. If a gas molecule is adsorbed at the surface of the detector, the resonance frequency of the crystal changes and this can be measured as a change in electrical properties. In addition, field effect transistors, used as
potentiometer A potentiometer is a three-terminal resistor with a sliding or rotating contact that forms an adjustable voltage divider. If only two terminals are used, one end and the wiper, it acts as a variable resistor or rheostat. The measuring instrum ...
s, can detect toxic gases if their gate is made sensitive to them.Ngo C., Van de Voorde M.H. (2014) Nanotechnology for Defense and Security. In: Nanotechnology in a Nutshell. Atlantis Press, Paris In a similar application, nanosensors can be utilized in military and law enforcement clothing and gear. The Navy Research Laboratory's Institute for Nanoscience has studied
quantum dot Quantum dots (QDs) are semiconductor particles a few nanometres in size, having optical and electronic properties that differ from those of larger particles as a result of quantum mechanics. They are a central topic in nanotechnology. When the ...
s for application in
nanophotonics Nanophotonics or nano-optics is the study of the behavior of light on the nanometer scale, and of the interaction of nanometer-scale objects with light. It is a branch of optics, optical engineering, electrical engineering, and nanotechnolog ...
and identifying biological materials. Nanoparticles layered with polymers and other receptor molecules will change color when contacted by analytes such as toxic gases. This alerts the user that they are in danger. Other projects involve embedding clothing with
biometric Biometrics are body measurements and calculations related to human characteristics. Biometric authentication (or realistic authentication) is used in computer science as a form of identification and access control. It is also used to identify in ...
sensors to relay information regarding the user's health and vitals, which would be useful for monitoring soldiers in combat. Surprisingly, some of the most challenging aspects in creating nanosensors for defense and military use are political in nature, rather than technical. Many different government agencies must work together to allocate budgets and share information and progress in testing; this can be difficult with such large and complex institutions. In addition, visas and immigration status can become an issue for foreign researchers - as the subject matter is very sensitive, government clearance can sometimes be required.Carafano, J. Nanotechnology and National Security: Small Changes, Big Impact. https://www.heritage.org/defense/report/nanotechnology-and-national-security-small-changes-big-impact (accessed Dec 3, 2018) Finally, there are currently not well defined or clear regulations on nanosensor testing or applications in the sensor industry, which contributes to the difficulty of implementation.


Food and the environment

Nanosensors can improve various sub-areas within food and environment sectors including food processing, agriculture, air and water quality monitoring, and packaging and transport.  Due to their sensitivity, as well as their tunability and resulting binding selectivity, nanosensors are very effective and can be designed for a wide variety of environmental applications. Such applications of nanosensors help in a convenient, rapid, and ultrasensitive assessment of many types of environmental pollutants. Chemical sensors are useful for analyzing odors from food samples and detecting atmospheric gases.  The "electronic nose" was developed in 1988 to determine the quality and freshness of food samples using traditional sensors, but more recently the sensing film has been improved with nanomaterials. A sample is placed in a chamber where volatile compounds become concentrated in the gas phase, whereby the gas is then pumped through the chamber to carry the aroma to the sensor that measures its unique fingerprint. The high surface area to volume ratio of the nanomaterials allows for greater interaction with analytes and the nanosensor's fast response time enables the separation of interfering responses. Chemical sensors, too, have been built using nanotubes to detect various properties of gaseous molecules. Many carbon nanotube based sensors are designed as field effect transistors, taking advantage of their sensitivity. The electrical conductivity of these nanotubes will change due to charge transfer and chemical doping by other molecules, enabling their detection. To enhance their selectivity, many of these involve a system by which nanosensors are built to have a specific pocket for another molecule. Carbon nanotubes have been used to sense
ionization Ionization, or Ionisation is the process by which an atom or a molecule acquires a negative or positive charge by gaining or losing electrons, often in conjunction with other chemical changes. The resulting electrically charged atom or molecul ...
of gaseous molecules while nanotubes made out of titanium have been employed to detect atmospheric concentrations of hydrogen at the molecular level. Some of these have been designed as field effect transistors, while others take advantage of optical sensing capabilities. Selective analyte binding is detected through spectral shift or fluorescence modulation. In a similar fashion, Flood et al. have shown that supramolecular host–guest chemistry offers quantitative sensing using Raman scattered light as well as
SERS Sers may refer to: * Sers, Armenia * Sers, Charente, France * Sers, Hautes-Pyrénées, France * Sers, Tunisia Sers, also Le Sers or Es Sers, is a town and commune in the Kef Governorate, Tunisia. It is located by road southeast of El Kef. As of ...
. Other types of nanosensors, including
quantum dot Quantum dots (QDs) are semiconductor particles a few nanometres in size, having optical and electronic properties that differ from those of larger particles as a result of quantum mechanics. They are a central topic in nanotechnology. When the ...
s and gold nanoparticles, are currently being developed to detect pollutants and toxins in the environment. These take advantage of the localized surface plasmon resonance (LSPR) that arises at the nanoscale, which results in wavelength specific absorption. This LSPR spectrum is particularly sensitive, and its dependence on nanoparticle size and environment can be used in various ways to design optical sensors. To take advantage of the LSPR spectrum shift that occurs when molecules bind to the nanoparticle, their surfaces can be functionalized to dictate which molecules will bind and trigger a response. For environmental applications, quantum dot surfaces can be modified with antibodies that bind specifically to microorganisms or other pollutants. Spectroscopy can then be used to observe and quantify this spectrum shift, enabling precise detection, potentially on the order of molecules. Similarly, fluorescent semiconducting nanosensors may take advantage of
fluorescence resonance energy transfer Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy ...
(FRET) to achieve optical detection. Quantum dots can be used as donors, and will transfer electronic excitation energy when positioned near acceptor molecules, thus losing their fluorescence. These quantum dots can be functionalized to determine which molecules will bind, upon which fluorescence will be restored. Gold nanoparticle-based optical sensors can be used to detect heavy metals very precisely; for example, mercury levels as low as 0.49 nanometers. This sensing modality takes advantage of FRET, in which the presence of metals inhibits the interaction between quantum dots and gold nanoparticles, and quenches the FRET response. Another potential implementation takes advantage of the size dependence of the LSPR spectrum to achieve ion sensing. In one study, Liu et al. functionalized gold nanoparticles with a Pb2+ sensitive enzyme to produce a lead sensor. Generally, the gold nanoparticles would aggregate as they approached each other, and the change in size would result in a color change. Interactions between the enzyme and Pb2+ ions would inhibit this aggregation, and thus the presence of ions could be detected. The main challenge associated with using nanosensors in food and the environment is determining their associated toxicity and overall effect on the environment. Currently, there is insufficient knowledge on how the implementation of nanosensors will affect the soil, plants, and humans in the long-term. This is difficult to fully address because nanoparticle toxicity depends heavily on the type, size, and dosage of the particle as well as environmental variables including pH, temperature, and humidity. To mitigate potential risk, research is being done to manufacture safe, nontoxic nanomaterials, as part of an overall effort towards green nanotechnology.


Healthcare

Nanosensors possess great potential for diagnostic medicine, enabling early identification of disease without reliance on observable symptoms. Ideal nanosensor implementations look to emulate the response of immune cells in the body, incorporating both diagnostic and immune response functionalities, while transmitting data to allow for monitoring of the sensor input and response. However, this model remains a long-term goal, and research is currently focused on the immediate diagnostic capabilities of nanosensors. The intracellular implementation of nanosensor synthesized with biodegradable polymers induces signals that enable real-time monitoring and thus paves way for advancement in drug delivery and treatment. One example of these nanosensors involves using the fluorescence properties of cadmium selenide
quantum dot Quantum dots (QDs) are semiconductor particles a few nanometres in size, having optical and electronic properties that differ from those of larger particles as a result of quantum mechanics. They are a central topic in nanotechnology. When the ...
s as sensors to uncover tumors within the body. A downside to the cadmium selenide dots, however, is that they are highly toxic to the body. As a result, researchers are working on developing alternate dots made out of a different, less toxic material while still retaining some of the fluorescence properties. In particular, they have been investigating the particular benefits of zinc sulfide quantum dots which, though they are not quite as fluorescent as cadmium selenide, can be augmented with other metals including manganese and various
lanthanide The lanthanide () or lanthanoid () series of chemical elements comprises the 15 metallic chemical elements with atomic numbers 57–71, from lanthanum through lutetium. These elements, along with the chemically similar elements scandium and yt ...
elements. In addition, these newer quantum dots become more fluorescent when they bond to their target cells. Another application of nanosensors involves using silicon nanowires in IV lines to monitor organ health. The nanowires are sensitive to detect trace biomarkers that diffuse into the IV line through blood which can monitor kidney or organ failure. These nanowires would allow for continuous biomarker measurement, which provides some benefits in terms of temporal sensitivity over traditional biomarker quantification assays such as ELISA. Nanosensors can also be used to detect contamination in organ implants. The nanosensor is embedded into the implant and detects contamination in the cells surrounding the implant through an electric signal sent to a clinician or healthcare provider. The nanosensor can detect whether the cells are healthy, inflammatory, or contaminated with bacteria. However, a main drawback is found within the long term use of the implant, where tissue grows on top of the sensors, limiting their ability to compress. This impedes the production of electrical charges, thus shortening the lifetime of these nanosensors, as they use the piezoelectric effect to self-power. Similarly to those used to measure atmospheric pollutants, gold-particle based nanosensors are used to give an early diagnosis to several types of cancer by detecting
volatile organic compound Volatile organic compounds (VOCs) are organic compounds that have a high vapour pressure at room temperature. High vapor pressure correlates with a low boiling point, which relates to the number of the sample's molecules in the surrounding air, a ...
s (VOCs) in breath, as tumor growth is associated with
peroxidation Lipid peroxidation is the chain of reactions of oxidative degradation of lipids. It is the process in which free radicals "steal" electrons from the lipids in cell membranes, resulting in cell damage. This process proceeds by a free radical cha ...
of the cell membrane. Another cancer related application, though still in mice probing stage, is the use of peptide-coated nanoparticles as activity-based sensors to detect lung cancer. The two main advantages of the use of nanoparticles to detect diseases is that it allows early stage detection, as it can detect tumors the size in the order of millimeters. It also provides a cost-effective, easy-to-use, portable, and non-invasive diagnostic tool. A recent effort towards advancement in nanosensor technology has employed
molecular imprinting Molecular imprinting is a technique to create template-shaped cavities in polymer matrices with predetermined selectivity and high affinity. This technique is based on the system used by enzymes for substrate recognition, which is called the " ...
, which is a technique used to synthesize polymer matrices that act as a receptor in molecular recognition. Analogous to the enzyme-substrate lock and key model, molecular imprinting uses template molecules with functional monomers to form polymer matrices with specific shape corresponding to its target template molecules, thus increasing the selectivity and affinity of the matrices. This technique has enabled nanosensors to detect chemical species. In the field of biotechnology, molecularly imprinted polymers (MIP) are synthesized receptors that have shown promising, cost-effective alternatives to natural antibodies in that they are engineered to have high selectivity and affinity. For example, an experiment with MI sensor containing nanotips with non-conductive
polyphenol Polyphenols () are a large family of naturally occurring organic compounds characterized by multiples of phenol units. They are abundant in plants and structurally diverse. Polyphenols include flavonoids, tannic acid, and ellagitannin, some ...
nano-coating (PPn coating) showed selective detection of E7 protein and thus demonstrated potential use of these nanosensors in detection and diagnosis of human papillomavirus, other human pathogens, and toxins. As shown above, nanosensors with molecular imprinting technique are capable of selectively detecting ultrasensitive chemical species in that by artificially modifying the polymer matrices, molecular imprinting increases the affinity and selectivity. Although molecularly imprinted polymers provide advantages in selective molecular recognition of nanosensors, the technique itself is relatively recent and there still remains challenges such as attenuation signals, detection systems lacking effective transducers, and surfaces lacking efficient detection. Further investigation and research on the field of molecularly imprinted polymers is crucial for development of highly effective nanosensors. In order to develop smart health care with nanosensors, a network of nanosensors, often called nanonetwork, need to be established to overcome the size and power limitations of individual nanosensors. Nanonetworks not only mitigates the existing challenges but also provides numerous improvements. Cell-level resolution of nanosensors will enable treatments to eliminate side effects, enable continuous monitoring and reporting of patients’ conditions. Nanonetworks require further study in that nanosensors are different from traditional sensors. The most common mechanism of sensor networks are through electromagnetic communications. However, the current paradigm is not applicable to nanodevices due to their low range and power. Optical signal transduction has been suggested as an alternative to the classical electromagnetic telemetry and has monitoring applications in human bodies. Other suggested mechanisms include bioinspired molecular communications, wired and wireless active transport in molecular communications, Forster energy transfer, and more. It is crucial to build an efficient nanonetwork so that it can be applied in fields such as medical implants, body area networks (BAN), internet of nano things (IoNT), drug delivery and more. With an adept nanonetwork, bio implantable nanodevices can provide higher accuracy, resolution, and safety compared to macroscale implants. Body area networks (BAN) enable sensors and actuators to collect physical and physiological data from the human body to better anticipate any diseases, which will thus facilitate the treatment. Potential applications of BAN include cardiovascular disease monitoring, insulin management, artificial vision and hearing, and hormonal therapy management. The Internet of Bio-Nano Things refers to networks of nanodevices that can be accessed by the internet. Development of IoBNT has paved the way to new treatments and diagnostic techniques. Nanonetworks may also help drug delivery by increasing localization and circulation time of drugs. Existing challenges with the aforementioned applications include biocompatibility of the nano implants, physical limitations leading to lack of power and memory storage, and bio compatibility of the transmitter and receiver design of IoBNT. The nanonetwork concept has numerous areas for improvements: these include developing
nanomachines A molecular machine, nanite, or nanomachine is a molecular component that produces quasi-mechanical movements (output) in response to specific stimuli (input). In cellular biology, macromolecular machines frequently perform tasks essential for l ...
, protocol stack issues, power provisioning techniques, and more. There are still stringent regulations in place for the development of standards for nanosensors to be used in the medical industry, due to insufficient knowledge of the adverse effects of nanosensors as well as potential cytotoxic effects of nanosensors. Additionally, there can be a high cost of raw materials such as silicon, nanowires, and carbon nanotubes, which prevent commercialization and manufacturing of nanosensors requiring scale-up for implementation. To mitigate the drawback of cost, researchers are looking into manufacturing nanosensors made of more cost-effective materials. There is also a high degree of precision needed to reproducibly manufacture nanosensors, due to their small size and sensitivity to different synthesis techniques, which creates additional technical challenges to be overcome.


See also

*
Nanotechnology Nanotechnology, also shortened to nanotech, is the use of matter on an atomic, molecular, and supramolecular scale for industrial purposes. The earliest, widespread description of nanotechnology referred to the particular technological goal ...
* List of nanotechnology topics *
Surface plasmon resonance Surface plasmon resonance (SPR) is the resonant oscillation of conduction electrons at the interface between negative and positive permittivity material in a particle stimulated by incident light. SPR is the basis of many standard tools for measu ...


References


External links


Weighing the Very Small: 'Nanobalance' Based on Carbon Nanotubes Shows New Application for Nanomechanics
Georgia Tech Research News.
Emerging Technologies and the Environment

Nanotechnology and Societal Transformation

Nanotechnology, Privacy and Shifting Social Conventions


{{emerging technologies, topics=yes, biomed=yes Nanotechnology Nanomedicine Emerging technologies