Pithoviridae

''Pithovirus'', first described in a 2014 paper, is a genus of giant virus known from two species, ''Pithovirus sibericum'', which infects amoebas and ''Pithovirus massiliensis''. It is a double-stranded DNA virus, and is a member of the nucleocytoplasmic large DNA viruses clade. The 2014 discovery was made when a viable specimen was found in a 30,000-year-old ice core harvested from permafrost in Siberia, Russia.

Description

The genus name ''Pithovirus'', a reference to large storage containers of ancient Greece known as pithoi, was chosen to describe the new species. A specimen of ''Pithovirus'' measures approximately 1.5  μm (1500 nm) in length and 0.5 μm (500 nm) in diameter, making it the largest virus yet found. It is 50% larger in size than the ''Pandoraviridae'', the previous largest-known viruses, and is larger than ''Ostreococcus'', the smallest eukaryotic cell, although ''Pandoravirus'' has the largest viral genome, containing 1.9 to 2.5 megabases of DNA.

''Pithovirus'' has a thick, oval wall with an opening at one end. Internally, its structure resembles a honeycomb.

The genome of ''Pithovirus'' contains 467 distinct genes, more than a typical virus but far fewer than the 2556 putative protein-coding sequences found in ''Pandoravirus''. Thus, its genome is far less densely packed than any other known virus. Two-thirds of its proteins are unlike those of other viruses. Despite the physical similarity with ''Pandoravirus'', the ''Pithovirus'' genome sequence reveals that it is barely related to that virus, but more closely resembles members of ''Marseilleviridae'', ''Megaviridae'', and ''Iridoviridae''. These families all contain large icosahedral viruses with DNA genomes. The ''Pithovirus'' genome has 36% GC-content, similar to the ''Megaviridae'', in contrast to greater than 61% for pandoraviruses.

Replication

''Pithovirus genome is one circular, double-stranded DNA ( dsDNA) chromosome of about 610,000 base pairs (bp), encoding approximately 467 open reading frames (ORFs), which translate into 467 different proteins. The genome encodes all the proteins needed to produce mRNA; these proteins are present in the purified virions. ''Pithovirus'' therefore undergoes its entire replication cycle in its host's cytoplasm, rather than the more typical method of taking over the host's nucleus.

Discovery

''Pithovirus sibericum'' was discovered in a 30,000-year-old sample of Siberian permafrost by Chantal Abergel and Jean-Michel Claverie of Aix-Marseille University. The virus was discovered buried below the surface of a late Pleistocene sediment. It was found when riverbank samples collected in 2000 were exposed to amoebas. The amoebas started dying and when examined were found to contain giant virus specimens. The authors said they got the idea to probe permafrost samples for new viruses after reading about an experiment that revived a similar aged seed of ''Silene stenophylla'' two years earlier. The ''Pithovirus'' findings were published in the ''Proceedings of the National Academy of Sciences'' in March 2014. Although the virus is harmless to humans, its viability after being frozen for millennia has raised concerns that global climate change and tundra drilling operations could lead to previously undiscovered and potentially pathogenic viruses being unearthed. However, other scientists dispute that this scenario poses a real threat.

A modern species in the genus, ''Pithovirus massiliensis'', was isolated in 2016. The core features like the order of ORFs and orphan genes (ORFans) are well conserved between the two known species.

Evolution

The rate of mutation of the genome has been estimated to be 2.23 × 10⁻⁶ substitutions/site/year. The authors have suggested that these viruses evolved at least hundreds of thousands of years ago.

See also

* DNA virus
* List of viruses
* Microbiology
* Virology
* Virus classification

References

External links

Viralzone: Pithovirus
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{{Taxonbar, from=Q15889043

Nucleocytoplasmic large DNA viruses
Discovered cryopreserved organisms
Unaccepted virus taxa