Nitrososphaera

''Nitrososphaera'' is a mesophilic genus of ammonia-oxidizing Crenarchaeota. The first ''Nitrososphaera'' organism was discovered in garden soils at the University of Vienna leading to the categorization of a new genus, family, order and class of Archaea. This genus is contains three distinct species: '' N. viennensis, Ca. N. gargensis'', and ''Ca N. evergladensis''. ''Nitrososphaera'' are chemolithoautotrophs and have important biogeochemical roles as nitrifying organisms.

Phylogeny

The ''Nitrososphaera'' genus contains one of the first discovered ammonia-oxidizing archaea (''N. viennensis)''. Only three distinct species of this genus have been identified. Both ''Ca. N. gargensis'', and ''Ca N. Evergladensis'' are known as ''Candidatus,'' which have been discovered and analyzed but have yet been studied in pure culture in a lab. The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LPSN) and National Center for Biotechnology Information (NCBI) Cladogram was taken from GTDB release 07-RS207 (8 April 2022).

Genome structure

The 16S rRNA gene of all ''Nitrososphaera'' ''sp.'' are nearly identical as they are neighboring within the phylogentic tree. ''N. viennensis'' has a 3% divergence from ''Ca. N. gargensis,'' while ''Ca. N evergladensis'' has a 97% similarity to ''Ca. N. gargensis'' within the 16S rRNA gene. The ''Nitrososphaera'' ''sp.'' use ammonia monooxygenase (''amo''A) genes to oxidize oxidize ammonium to nitrite.

Morphology

All three species contain genes for urease, urea, and ammonia. Nitrososphaera have a cell membrane composed of crenarchaeol, its isomer, and a glycerol dialkyl glycerol tetraether (GDGT), all of which are used for identifying ammonia-oxidizing archaea. ''N. viennensis'' has a cell diameter of 0.6–0.9 µm and is an irregular spherical coccus. ''Ca. N. gargensis'' is non-pathogenic presents a diameter of approximately 0.9 ± 0.3 μm with a relatively small coccus. ''Ca. N evergladensis'' has yet to be properly analyzed and described for morphological characteristics.  

Habitats

Ammonia-oxidizing archaea have been found in various environments and habitats around the world. ''N. viennensis'' was first discovered in garden soils. The preferred growth conditions are 35 °C - 42 °C and pH of 7.5. ''Ca. N. gargensis'' was found in hot springs and is commonly found in heavy metal containing habitats with a growth temperature of ~ 46 °C. ''Ca. N evergladensis'' was first discovered in the humid region of the Everglades in Florida. Other relatives of ''Nitrososphaera sp.'' have also been detected in swamps, microbial mats, freshwater sediments, deep sea marine sediments, and regions with high levels of nitrogen and ammonia sources to allow for the oxidation process of the lipids and nutrients for the optimal survival of these microbes.

Nitrification and environmental impact

The discovery of ''Nitrososphaera'' capable of ammonia oxidation indicated that both archaea and bacteria were capable of ammonia oxidation. Ammonia-oxidizing archaea have been comparable to ammonia-oxidizing bacteria. It was not until recent discovery and analysis, scientists believed that only ammonia-oxidizing bacteria were capable of oxidizing ammonia within the soils. However, ammonia-oxidizing archaea and ammonia-oxidizing bacteria work together in the nitrogen cycle. Ammonia-oxidizing archaea, including ''Nitrososphaera,'' are abundant in warm and humid soils, along with ammonia-oxidizing bacteria. Both microbes play a significant role in the nitrification of soils.

''Nitrososphaera'' utilize ammonia from the environment to generate ATP by oxidizing ammonia (NH₃) into nitrite (NO₂⁻). Ammonia oxidation leads to the disaggregation of other chemical compounds, providing important nutrients for plant survival. One of the chemical compounds that forms from nitrogen cycling is nitrous oxide (N₂O), a greenhouse gas. Nitrous oxide has a 216 times higher radiative efficiency than CO₂. These ammonia-oxidizing archaea are a key component in soils, which emit more than 65% of the Earth's atmospheric nitrous oxide concentrations.

References

Further reading

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Archaea taxonomic orders
Archaea