''Nitrobacter'' is a genus comprising rod-shaped, gram-negative, and chemoautotrophic bacteria. The name ''Nitrobacter'' derives from the Latin neuter gender noun ''nitrum, nitri'', alkalis; the Ancient Greek noun βακτηρία'','' βακτηρίᾱς'','' rod. They are non-motile and reproduce via budding or binary fission. ''Nitrobacter'' cells are obligate aerobes and have a doubling time of about 13 hours. ''Nitrobacter'' play an important role in the nitrogen cycle by oxidizing nitrite into nitrate in soil and marine systems. Unlike plants, where electron transfer in photosynthesis provides the energy for carbon fixation, ''Nitrobacter'' uses energy from the oxidation of nitrite ions, NO2, into nitrate ions, NO3, to fulfill their energy needs. ''Nitrobacter'' fix carbon dioxide via the Calvin cycle for their carbon requirements. ''Nitrobacter'' belongs to the α-subclass of the Proteobacteria.

Morphology and Characteristics

''Nitrobacter'' are gram-negative bacteria and are either rod-shaped, pear-shaped or pleomorphic. They are typically 0.5–0.9 μm in width and 1.0–2.0 μm in length and have an intra-cytomembrane polar cap. Due to the presence of cytochromes c, they are often yellow in cell suspensions. The nitrate oxidizing system on membranes is cytoplasmic. ''Nitrobacter'' cells have been shown to recover following extreme carbon dioxide exposure and are non-motile.


16s rRNA sequence analysis phylogenetically places ''Nitrobacter'' within the class of Alphaproteobacteria. Pairwise evolutionary distance measurements within the genus are low compared to those found in other genera, and are less than 1%. ''Nitrobacter'' are also closely related to other species within the alpha subdivision, including the photosynthetic ''Rhodopseudomonas palustris'', the root-nodulating ''Bradyrhizobium japonicum'' and ''Blastobacter denitrificans'', and the human pathogens ''Afipia felis'' and ''Afipia clevelandensis.'' Bacteria within the genus ''Nitrobacter'' are presumed to have arose on multiple occasions from a photosynthetic ancestor, and for individual nitrifying genera and species there is evidence that the nitrification phenotype evolved separately from that found in photosynthetic bacteria. All known nitrite-oxidizing prokaryotes are restricted to a handful of phylogenetic groups. This includes the genus ''Nitrospira'' within the phylum Nitrospirae, and the genus ''Nitrolancetus ''from the phylum Chloroflexi. Before 2004, nitrite oxidation was believed to only occur within Proteobacteria; it is likely that further scientific inquiry will expand the list of known nitrite-oxidizing species. The low diversity of species oxidizing nitrite oxidation contrasts with other processes associated with the nitrogen cycle in the ocean, such as denitrification and N-fixation, where a diverse range of taxa perform analogous functions.


Main article: Nitrification

Nitrification is a crucial component of the nitrogen cycle, especially in the oceans. The production of nitrate (NO3) by oxidation of nitrite (NO2) by nitrification the process that produces the inorganic nitrogen that supplies much of the demand by marine oxygenic, photosynthetic organisms such as phytoplankton, particularly in areas of upwelling. For this reason, nitrification supplies much of the nitrogen that fuels planktonic primary production in the world's oceans. Nitrification is estimated to be the source of half of the nitrate consumed by phytoplankton globally. Phytoplankton are major contributors to oceanic production, and are therefore important for the biological pump which exports carbon and other particulate organic matter from the surface waters of the world's oceans. The process of nitrification is crucial for separating recycled production from production leading to export. Biologically metabolized nitrogen returns to the inorganic dissolved nitrogen pool in the form of ammonia. Microbe-mediated nitrification converts that ammonia into nitrate, which can subsequently be taken up by phytoplankton and recycled. The nitrite oxidation reaction performed by the ''Nitrobacter'' is as follows; NO2 + H2O → NO3 + 2H+ + 2e 2H+ + 2e + ½O2 → H2O The Gibbs' Free Energy yield for nitrite oxidation is: ΔGο = -74 kJ mol−1 NO2 In the oceans, nitrite-oxidizing bacteria such as ''Nitrobacter'' are usually found in close proximity to ammonia-oxidizing bacteria. These two reactions together make up the process of nitrification. The nitrite-oxidation reaction generally proceeds more quickly in ocean waters, and therefore is not a rate-limiting step in nitrification. For this reason, it is rare for nitrite to accumulate in ocean waters. The two-step conversion of ammonia to nitrate observed in ammonia-oxidizing bacteria, ammonia-oxidizing archaea and nitrite-oxidizing bacteria (such as ''Nitrobacter'') is puzzling to researchers. Complete nitrification, the conversion of ammonia to nitrate in a single step known as comammox, has an energy yield (∆G°′) of −349 kJ mol−1 NH3, while the energy yields for the ammonia-oxidation and nitrite-oxidation steps of the observed two-step reaction are −275 kJ mol−1 NH3, and −74 kJ mol−1 NO2, respectively. These values indicate that it would be energetically favourable for an organism to carry out complete nitrification from ammonia to nitrate (comammox), rather than conduct only one of the two steps. The evolutionary motivation for a decoupled, two-step nitrification reaction is an area of ongoing research. In 2015, it was discovered that the ''species Nitrospira inopinata'' possesses all the enzymes required for carrying out complete nitrification in one step, suggesting that this reaction does occur. This discovery raises questions about evolutionary capability of ''Nitrobacter'' to conduct only nitrite-oxidation.

Metabolism and Growth

Members of the genus ''Nitrobacter'' use nitrite as a source of electrons (reductant), O2 as a source of energy, Schmidt-Rohr, K. (2020). "Oxygen Is the High-Energy Molecule Powering Complex Multicellular Life: Fundamental Corrections to Traditional Bioenergetics” ''ACS Omega'' 5: 2221-2233. http://dx.doi.org/10.1021/acsomega.9b03352 and CO2 as a carbon source. Nitrite is not a particularly favourable substrate from which to gain energy. Thermodynamically, nitrite oxidation gives a yield (∆G°′) of only -74  kJ mol−1 NO2. As a result, ''Nitrobacter'' has developed a highly specialized metabolism to derive energy from the oxidation of nitrite. Cells in the genus ''Nitrobacter'' reproduce by budding or binary fission. Carboxysomes, which aid carbon fixation, are found in lithoautotrophically and mixotrophically grown cells. Additional energy conserving inclusions are PHB granules and polyphosphates. When both nitrite and organic substances are present, cells can exhibit biphasic growth; first the nitrite is used and after a lag phase, organic matter is oxidized. Chemoorganotroph growth is slow and unbalanced, thus more poly-β-hydroxybutyrate granules are seen that distort the shape and size of the cells. The enzyme responsible for the oxidation of nitrite to nitrate in members of the genus ''Nitrobacter'' is nitrite oxidoreductase (NXR), which is encoded by the gene ''nxrA''. NXR is composed of two subunits, and likely forms an αβ-heterodimer. The enzyme exists within the cell on specialized membranes in the cytoplasm that can be folded into vesicles or tubes. The α-subunit is thought to be the location of nitrite oxidation, and the β-subunit is an electron channel from the membrane. The direction of the reaction catalyzed by NXR can be reversed depending on oxygen concentrations. The region of the ''nxrA'' gene which encodes for the β-subunit of the NXR enzyme is similar in sequence to the iron-sulfur centers of bacterial ferredoxins, and to the β-subunit of the enzyme nitrate reductase, found in ''Escherichia coli''.

Ecology and Distribution

The genus ''Nitrobacter'' is widely distributed in both aquatic and terrestrial environments. Nitrifying bacteria have an optimum growth between 25 and 30 °C, and cannot survive past the upper limit of 49 °C or the lower limit of 0 °C. This limits their distribution even though they can be found in a wide variety of habitats. Cells in the genus ''Nitrobacter'' have an optimum pH for growth between 7.3 and 7.5, and will die at temperatures exceeding 120 °F (49 °C) or below 32 °F (0 °C). According to Grundmann, ''Nitrobacter'' seem to grow optimally at 38 °C and at a pH of 7.9, but Holt states that ''Nitrobacter'' grow optimally at 28 °C and within a pH range of 5.8 to 8.5, although they have a pH optima between 7.6 and 7.8. The primary ecological role of members of the genus ''Nitrobacter'' is to oxidize nitrite to nitrate, a primary source on inorganic nitrogen for plants. This role is also essential in aquaponics. Since all members in the genus ''Nitrobacter'' are obligate aerobes, oxygen along with phosphorus tend to be factors that limit their capability to perform nitrite oxidation. One of the major impacts of nitrifying bacteria such as ammonia-oxidizing ''Nitrosomonas'' and nitrite-oxidizing ''Nitrobacter'' in both oceanic and terrestrial ecosystems is on the process of eutrophication. The distribution and differences in nitrification rates across different species of ''Nitrobacter'' may be attributed to differences in the plasmids among species, as data presented in Schutt (1990) imply, habitat-specific plasmid DNA was induced by adaptation for some of the lakes that were investigated. A follow-up study performed by Navarro ''et al''. (1995) showed that various ''Nitrobacter'' populations carry two large plasmids. In conjunction with Schutts’ (1990) study, Navarro et al. (1995) illustrated genomic features that may play crucial roles in determining the distribution and ecological impact of members of the genus ''Nitrobacter''. Nitrifying bacteria in general tend to be less abundant than their heterotrophic counterparts, as the oxidizing reactions they perform have a low energy yield and most of their energy production goes toward carbon-fixation rather than growth and reproduction.


In 1890, Ukrainian-Russian microbiologist Sergei Winogradsky isolated the first pure cultures of nitrifying bacteria which are capable of growth in the absence of organic matter and sunlight. The exclusion of organic material by Winogradsky in the preparation of his cultures is recognized as a contributing factor to his success in isolating the microbes. In 1891, English chemist Robert Warington proposed a two-stage mechanism for nitrification, mediated by two distinct genera of bacteria. The first stage proposed was the conversion of ammonia to nitrite, and the second the oxidation of nitrite to nitrate. Winogradsky named the bacteria responsible for the oxidation of nitrite to nitrate ''Nitrobacter'' in his subsequent study on microbial nitrification in 1892. Winslow et al. proposed the type species ''Nitrobacter winogradsky'' in 1917. The species was officially recognized in 1980.

Main Species

* ''Nitrobacter winogradskyi'' * ''Nitrobacter hamburgensis'' * ''Nitrobacter vulgaris'' * ''Nitrobacter alkalicus''

See also

* Nitrate * Nitrite * Nitrobacteraceae * Nitrogen cycle * Nitrosomonas * Nitrospira * Nitrospirae


{{Taxonbar|from=Q146274 Category:Bacteria genera Category:Bradyrhizobiaceae