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Nitrifying Bacteria
Nitrifying bacteria are chemolithotrophic organisms that include species of genera such as ''Nitrosomonas'', ''Nitrosococcus'', ''Nitrobacter'', '' Nitrospina'', ''Nitrospira'' and '' Nitrococcus''. These bacteria get their energy from the oxidation of inorganic nitrogen compounds. Types include ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB). Many species of nitrifying bacteria have complex internal membrane systems that are the location for key enzymes in nitrification: ammonia monooxygenase (which oxidizes ammonia to hydroxylamine), hydroxylamine oxidoreductase (which oxidizes hydroxylamine to nitric oxide - which is further oxidized to nitrite by a currently unidentified enzyme), and nitrite oxidoreductase (which oxidizes nitrite to nitrate). Ecology Nitrifying bacteria are present in distinct taxonomical groups and are found in highest numbers where considerable amounts of ammonia are present (such as areas with extensive protein decomposition, and sewage ...
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Lithotroph
Lithotrophs are a diverse group of organisms using an inorganic substrate (usually of mineral origin) to obtain reducing equivalents for use in biosynthesis (e.g., carbon dioxide fixation) or energy conservation (i.e., ATP production) via aerobic or anaerobic respiration. While lithotrophs in the broader sense include photolithotrophs like plants, chemolithotrophs are exclusively microorganisms; no known macrofauna possesses the ability to use inorganic compounds as electron sources. Macrofauna and lithotrophs can form symbiotic relationships, in which case the lithotrophs are called "prokaryotic symbionts". An example of this is chemolithotrophic bacteria in giant tube worms or plastids, which are organelles within plant cells that may have evolved from photolithotrophic cyanobacteria-like organisms. Chemolithotrophs belong to the domains Bacteria and Archaea. The term "lithotroph" was created from the Greek terms 'lithos' (rock) and 'troph' (consumer), meaning "eaters of rock" ...
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Nitrite
The nitrite polyatomic ion, ion has the chemical formula . Nitrite (mostly sodium nitrite) is widely used throughout chemical and pharmaceutical industries. The nitrite anion is a pervasive intermediate in the nitrogen cycle in nature. The name nitrite also refers to organic compounds having the –ONO group, which are esters of nitrous acid. Production Sodium nitrite is made industrially by passing a mixture of nitrogen oxides into aqueous sodium hydroxide or sodium carbonate solution: : The product is purified by recrystallization. Alkali metal nitrites are thermally stable up to and beyond their melting point (441 °C for KNO2). Ammonium nitrite can be made from dinitrogen trioxide, N2O3, which is formally the anhydride of nitrous acid: :2 NH3 + H2O + N2O3 → 2 NH4NO2 Structure The nitrite ion has a symmetrical structure (C2v molecular point group, symmetry), with both N–O bonds having equal length and a bond angle of about 115°. In valence bond theory, it is des ...
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Ammonia Oxidation
Ammonia is an inorganic compound of nitrogen and hydrogen with the formula . A stable binary hydride, and the simplest pnictogen hydride, ammonia is a colourless gas with a distinct pungent smell. Biologically, it is a common nitrogenous waste, particularly among aquatic organisms, and it contributes significantly to the nutritional needs of terrestrial organisms by serving as a precursor to 45% of the world's food and fertilizers. Around 70% of ammonia is used to make fertilisers in various forms and composition, such as urea and Diammonium phosphate. Ammonia in pure form is also applied directly into the soil. Ammonia, either directly or indirectly, is also a building block for the synthesis of many pharmaceutical products and is used in many commercial cleaning products. It is mainly collected by downward displacement of both air and water. Although common in nature—both terrestrially and in the outer planets of the Solar System—and in wide use, ammonia is both causti ...
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Comammox
Comammox (COMplete AMMonia OXidation) is the name attributed to an organism that can convert ammonia into nitrite and then into nitrate through the process of nitrification. Nitrification has traditionally thought to be a two-step process, where ammonia-oxidizing bacteria and archaea oxidize ammonia to nitrite and then nitrite-oxidizing bacteria convert to nitrate. Complete conversion of ammonia into nitrate by a single microorganism was first predicted in 2006. In 2015 the presence of microorganisms that could carry out both conversion processes was discovered within the genus ''Nitrospira,'' and the nitrogen cycle was updated. Within the genus ''Nitrospira,'' the major ecosystems comammox are primarily found in natural aquifers and engineered ecosystems. Complete nitrification step yield more energy (∆G°′ = −349 kJ mol−1 NH3) than either single oxidation alone (∆G°′ = −275 kJ mol−1 NH3 for ammonia oxidation to nitrite and ∆G°′ = −74 kJ ...
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Sergei Winogradsky
Sergei Nikolaievich Winogradsky (or Vinohradsky; published under the name of Sergius Winogradsky or M. S. Winogradsky from Ukrainian Mykolayovych Serhiy; uk, Сергій Миколайович Виноградський; 1 September 1856 – 25 February 1953) was a Ukrainian microbiologist, ecologist and soil scientist who pioneered the cycle-of-life concept. Winogradsky discovered the first known form of lithotrophy during his research with '' Beggiatoa'' in 1887. He reported that ''Beggiatoa'' oxidized hydrogen sulfide (H2S) as an energy source and formed intracellular sulfur droplets. This research provided the first example of lithotrophy, but not autotrophy. His research on nitrifying bacteria would report the first known form of chemoautotrophy, showing how a lithotroph fixes carbon dioxide (CO2) to make organic compounds. He is best known in school science as the inventor of the Winogradsky Column technique for the study of sediment microbes. Biography Winograds ...
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Microbiologist
A microbiologist (from Ancient Greek, Greek ) is a scientist who studies microscopic life forms and processes. This includes study of the growth, interactions and characteristics of Microorganism, microscopic organisms such as bacteria, algae, fungi, and some types of parasites and their vectors. Most microbiologists work in offices and/or research facilities, both in private biotechnology companies and in academia. Most microbiologists specialize in a given topic within microbiology such as bacteriology, parasitology, virology, or immunology. Duties Microbiologists generally work in some way to increase scientific knowledge or to utilise that knowledge in a way that improves outcomes in medicine or some industry. For many microbiologists, this work includes planning and conducting experimental research projects in some kind of laboratory setting. Others may have a more administrative role, supervising scientists and evaluating their results. Microbiologists working in the medi ...
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Chloroflexota
The Chloroflexota are a phylum of bacteria containing isolates with a diversity of phenotypes, including members that are aerobic thermophiles, which use oxygen and grow well in high temperatures; anoxygenic phototrophs, which use light for photosynthesis (green non-sulfur bacteria); and anaerobic halorespirers, which uses halogenated organics (such as the toxic chlorinated ethenes and polychlorinated biphenyls) as electron acceptors. The members of the phylum ''Chloroflexota'' are monoderms (that is, have one cell membrane with no outer membrane), but they stain mostly gram-negative. Many well-studied phyla of bacteria are diderms and stain gram-negative, whereas well-known monoderms that stain Gram-positive include ''Firmicutes'' (or ''Bacillota'') ( low G+C gram-positives), ''Actinomycetota'' (high-G+C gram-positives) and ''Deinococcota'' (gram-positive diderms with thick peptidoglycan). History The taxon name was created in the 2001 edition of Volume 1 of Bergey's Manual of ...
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Pseudomonadota
Pseudomonadota (synonym Proteobacteria) is a major phylum of Gram-negative bacteria. The renaming of phyla in 2021 remains controversial among microbiologists, many of whom continue to use the earlier names of long standing in the literature. The phylum Proteobacteria includes a wide variety of pathogenic genera, such as ''Escherichia'', '' Salmonella'', ''Vibrio'', ''Yersinia'', ''Legionella'', and many others.Slonczewski JL, Foster JW, Foster E. Microbiology: An Evolving Science 5th Ed. WW Norton & Company; 2020. Others are free-living (nonparasitic) and include many of the bacteria responsible for nitrogen fixation. Carl Woese established this grouping in 1987, calling it informally the "purple bacteria and their relatives". Because of the great diversity of forms found in this group, it was later informally named Proteobacteria, after Proteus, a Greek god of the sea capable of assuming many different shapes (not after the Proteobacteria genus ''Proteus''). In 2021 the Internat ...
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Nitrospirota
Nitrospirota is a phylum of bacteria. It includes multiple genera, such as ''Nitrospira'', the largest. The first member of this phylum, ''Nitrospira marina'', was discovered in 1985. The second member, ''Nitrospira moscoviensis'', was discovered in 1995. Nitrospirota contains nitrifying taxa which oxidize nitrite to nitrate (nitrite-oxidizing bacteria, NOB) and commamox bacteria ''Nitrospira inopinata'' discovered in 2015 and cultivated in 2017. Phylogeny Taxonomy The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature (LSPN) and the National Center for Biotechnology Information (NCBI). * Phylum "Nitrospirota" Garrity & Holt 2021 ** Class " Leptospirillia" *** Order " Leptospirillales" **** Family " Leptospirillaceae" Cavalier-Smith 2020 ***** Genus '' Leptospirillum'' Markosyan 1972 ex Hippe 2000 ****** Species "'' L. ferrodiazotrophum''" Tyson et al. 2005 ****** Species '' L. ferriphilum'' Coram & Rawlings 2002 ****** Speci ...
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Nitrospinota
Nitrospinota is a bacterial phylum. Despite only few described species, members of this phylum are major nitrite-oxidizing bacteria in surface waters in oceans. By oxidation of nitrite to nitrate they are important in the process of nitrification in marine environments. Although genus Nitrospina is aerobic bacterium, it was shown to oxidize nitrite also in oxygen minimum zone of the ocean. Depletion of oxygen in such zones leads to preference of anaerobic processes such as denitrification and nitrogen loss through anammox. Nitrospina thus outweight nitrogen loss by nitrification also in these oxygen depleted zones. Among the cultivated isolates within the genus ''Nitrospina'' are '' Nitrospina gracilis'' and '' Nitrospina watsonii''. Further genomes were resolved by culture-independent metagenome binning. The two ''Nitrospina'' species are, however, distantly related to environmentally abundant uncultured Nitrospinota. The two other strains were cultivated in 2020 each in the ...
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Archaea
Archaea ( ; singular archaeon ) is a domain of single-celled organisms. These microorganisms lack cell nuclei and are therefore prokaryotes. Archaea were initially classified as bacteria, receiving the name archaebacteria (in the Archaebacteria kingdom), but this term has fallen out of use. Archaeal cells have unique properties separating them from the other two domains, Bacteria and Eukaryota. Archaea are further divided into multiple recognized phyla. Classification is difficult because most have not been isolated in a laboratory and have been detected only by their gene sequences in environmental samples. Archaea and bacteria are generally similar in size and shape, although a few archaea have very different shapes, such as the flat, square cells of ''Haloquadratum walsbyi''. Despite this morphological similarity to bacteria, archaea possess genes and several metabolic pathways that are more closely related to those of eukaryotes, notably for the enzymes involved ...
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Gammaproteobacteria
Gammaproteobacteria is a class of bacteria in the phylum Pseudomonadota (synonym Proteobacteria). It contains about 250 genera, which makes it the most genera-rich taxon of the Prokaryotes. Several medically, ecologically, and scientifically important groups of bacteria belong to this class. It is composed by all Gram-negative microbes and is the most phylogenetically and physiologically diverse class of Proteobacteria. These microorganisms can live in several terrestrial and marine environments, in which they play various important roles, including ''extreme environments'' such as hydrothermal vents. They generally have different shapes - rods, curved rods, cocci, spirilla, and filaments and include free living bacteria, biofilm formers, commensals and symbionts, some also have the distinctive trait of being bioluminescent. Metabolisms found in the different genera are very different; there are both aerobic and anaerobic (obligate or facultative) species, chemolithoautotrophic ...
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