Lactobacillus

''Lactobacillus'' is a genus of gram-positive, aerotolerant anaerobes or microaerophilic, rod-shaped, non-spore-forming bacteria. Until 2020, the genus ''Lactobacillus'' comprised over 260 phylogenetically, ecologically, and metabolically diverse species; a taxonomic revision of the genus assigned lactobacilli to 25 genera (see below).

''Lactobacillus'' species constitute a significant component of the human and animal microbiota at a number of body sites, such as the digestive system and the female genital system. In women of European ancestry, ''Lactobacillus'' species are normally a major part of the vaginal microbiota. ''Lactobacillus'' forms biofilms in the vaginal and gut microbiota, allowing them to persist in harsh environmental conditions and maintain ample populations. ''Lactobacillus'' exhibits a mutualistic relationship with the human body, as it protects the host against potential invasions by pathogens, and in turn, the host provides a source of nutrients. Lactobacilli are among the most common probiotic found in food such as yogurt, and the bacteria are diverse in their application in maintaining human well-being, by helping to treat diarrhea, vaginal infections, and skin disorders such as eczema.

Metabolism

Lactobacilli are homofermentative, i.e., hexoses are metabolized by glycolysis to lactate as the major end product, or heterofermentative, i.e., hexoses are metabolized by the Phosphoketolase pathway to lactate, CO₂, and acetate or ethanol as major end products. Most lactobacilli are aerotolerant and some species respire if heme and menaquinone are present in the growth medium. Aerotolerance of lactobacilli is manganese-dependent and has been explored (and explained) in ''Lactiplantibacillus plantarum'' (previously '' Lactobacillus plantarum)''. Lactobacilli generally do not require iron for growth.

The ''Lactobacillaceae'' are the only family of the lactic acid bacteria (LAB) that includes homofermentative and heterofermentative organisms; in the ''Lactobacillaceae'', homofermentative or heterofermentative metabolism is shared by all strains of a genus. ''Lactobacillus'' species are all homofermentative, do not express pyruvate formate lyase, and most species do not ferment pentoses. In ''L. crispatus'', pentose metabolism is strain specific and acquired by lateral gene transfer.

Genomes

The genomes of lactobacilli are highly variable, ranging in size from 1.2 to 4.9 Mb ( megabases). Accordingly, the number of protein-coding genes ranges from 1,267 to about 4,758 genes (in ''Fructilactobacillus sanfranciscensis'' and ''Lentilactobacillus parakefiri'', respectively). Even within a single species, there can be substantial variation. For instance, strains of ''L. crispatus'' have genome sizes ranging from 1.83 to 2.7 Mb, or 1,839 to 2,688 open reading frames. ''Lactobacillus'' contains a wealth of compound microsatellites in the coding region of the genome, which are imperfect and have variant motifs. Many lactobacilli also contain multiple plasmids. A recent study has revealed that plasmids encode the genes which are required for adaptation of lactobacilli to the given environment.

Species

The genus ''Lactobacillus'' comprises the following species:

* '' Lactobacillus acetotolerans'' Entani et al. 1986

* '' Lactobacillus acidophilus'' (Moro 1900) Hansen and Mocquot 1970 (Approved Lists 1980)

* "''Lactobacillus alvi''" Kim et al. 2011
* '' Lactobacillus amylolyticus'' Bohak et al. 1999

* '' Lactobacillus amylovorus'' Nakamura 1981

* '' Lactobacillus apis'' Killer et al. 2014

* "''Lactobacillus backi''" Bohak et al. 2006

* '' Lactobacillus bombicola'' Praet et al. 2015

* '' Lactobacillus colini'' Zhang et al. 2017

* '' Lactobacillus crispatus'' (Brygoo and Aladame 195555) Moore and Holdeman 1970 (Approved Lists 1980)

* '' Lactobacillus delbrueckii'' (Leichmann 1896) Beijerinck 1901 (Approved Lists 1980)

* '' Lactobacillus equicursoris'' Morita et al. 2010

* '' Lactobacillus fornicalis'' Dicks et al. 2000

* '' Lactobacillus gallinarum'' Fujisawa et al. 1992

* '' Lactobacillus gasseri'' Lauer and Kandler 1980

* '' Lactobacillus gigeriorum'' Cousin et al. 2012
* "''Lactobacillus ginsenosidimutans''" Jung et al. 2013

* '' Lactobacillus hamsteri'' Mitsuoka and Fujisawa 1988

* '' Lactobacillus helsingborgensis'' Olofsson et al. 2014
* '' Lactobacillus helveticus'' (Orla-Jensen 1919) Bergey et al. 1925 (Approved Lists 1980)

* '' Lactobacillus hominis'' Cousin et al. 2013

* '' Lactobacillus iners'' Falsen et al. 1999

* '' Lactobacillus intestinalis'' (ex Hemme 1974) Fujisawa et al. 1990

* '' Lactobacillus jensenii'' Gasser et al. 1970 (Approved Lists 1980)

* "''Lactobacillus jinshani''" Yu et al. 2020

* '' Lactobacillus johnsonii'' Fujisawa et al. 1992

* '' Lactobacillus kalixensis'' Roos et al. 2005

* ''Lactobacillus kefiranofaciens'' Fujisawa et al. 1988

* '' Lactobacillus kimbladii'' Olofsson et al. 2014

* '' Lactobacillus kitasatonis'' Mukai et al. 2003

* '' Lactobacillus kullabergensis'' Olofsson et al. 2014

* '' Lactobacillus melliventris'' Olofsson et al. 2014

* '' Lactobacillus mulieris'' Rocha et al. 2020

* '' Lactobacillus nasalidis'' Suzuki-Hashido et al. 2021

* '' Lactobacillus panisapium'' Wang et al. 2018

* '' Lactobacillus paragasseri'' Tanizawa et al. 2018

* '' Lactobacillus pasteurii'' Cousin et al. 2013

* '' Lactobacillus porci'' Kim et al. 2018

* '' Lactobacillus psittaci'' Lawson et al. 2001

* "''Lactobacillus raoultii''" Nicaise et al. 2018

* '' Lactobacillus rodentium'' Killer et al. 2014
* '' Lactobacillus rogosae'' Holdeman and Moore 1974 (Approved Lists 1980)

* '' Lactobacillus taiwanensis'' Wang et al. 2009

* "'' Lactobacillus thermophilus''" Ayers and Johnson 1924

* "''Lactobacillus timonensis''" Afouda et al. 2017

* '' Lactobacillus ultunensis'' Roos et al. 2005

* '' Lactobacillus xujianguonis'' Meng et al. 2020

Taxonomy

The genus ''Lactobacillus'' currently contains 44 species which are adapted to vertebrate hosts or to insects. In recent years, other members of the genus ''Lactobacillus'' (formerly known as the ''Leuconostoc'' branch of ''Lactobacillus'') have been reclassified into the genera '' Atopobium'', '' Carnobacterium'', '' Weissella'', '' Oenococcus'', and '' Leuconostoc''. The '' Pediococcus'' species '' P. dextrinicus'' has been reclassified as a ''Lapidilactobacillus dextrinicus'' and most lactobacilli were assigned to '' Paralactobacillus'' or one of the 23 novel genera of the ''Lactobacillaceae.'' Two websites inform on the assignment of species to the novel genera or species (http://www.lactobacillus.uantwerpen.be/; http://www.lactobacillus.ualberta.ca/).

Phylogeny

The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature and the phylogeny is based on whole-genome sequences.

Human health

Vaginal tract

''Lactobacillus'' s.s. species are considered "keystone species" in the vaginal flora of reproductive-age women. Most, but not all, healthy women have vaginal floras dominated by one of four species of ''Lactobacillus'': ''L. iners'', ''L. crispatus'', ''L. gasseri'', and ''L. jensenii''. Other women have a more diverse mix of anaerobic microorganisms and are still considered to have a healthy microbiome.

Interactions with pathogens

Lactobacilli produce lactic acid, which contributes to the vaginal acidity, and this lowered pH is generally accepted to be the main mechanism controlling the composition of the vaginal microflora.

Lactobacilli are also proposed to produce hydrogen peroxide, which inhibits the growth and virulence of the fungal pathogen '' Candida albicans'' ''in vitro'', though this is arguably not the main mechanism ''in vivo''.

''In vitro'' studies have also shown that lactobacilli reduce the pathogenicity of ''C. albicans'' through the production of organic acids and certain metabolites. Both the presence of metabolites, such as sodium butyrate, and decrease in environmental pH caused by the organic acids reduce the growth of hyphae in ''C. albicans'', which reduces its pathogenicity. Lactobacilli also reduce the pathogenicity of ''C. albicans'' by reducing ''C. albicans'' biofilm formation. On the other hand, following antibiotic therapy, certain ''Candida'' species can suppress the regrowth of lactobacilli at body sites where they cohabitate, such as in the gastrointestinal tract.

In addition to its effects on ''C. albicans'', ''Lactobacillus'' sp. also interact with other pathogens. For example, '' Limosilactobacillus reuteri'' (formerly ''Lactobacillus reuteri'') can inhibit the growth of many different bacterial species by using glycerol to produce the antimicrobial substance called reuterin. Another example is '' Ligilactobacillus salivarius (''formerly ''Lactobacillus salivarius''), which interacts with many pathogens through the production of salivaricin B, a bacteriocin.

Probiotics

Because of the interactions with other microbes, fermenting bacteria like lactic acid bacteria (LAB) are now in use as probiotics with many applications.

Lactobacilli administered in combination with other probiotics provides benefits in cases of irritable bowel syndrome (IBS), although the extent of efficacy is still uncertain. The probiotics help treat IBS by re-establishing homeostasis when the gut microbiota experiences unusually high levels of opportunistic bacteria. In addition, lactobacilli can be administered as probiotics during cases of infection by the ulcer-causing bacterium ''Helicobacter pylori''. ''Helicobacter pylori'' is linked to cancer, and antibiotic resistance impedes the success of current antibiotic-based eradication treatments. When probiotic lactobacilli are administered along with the treatment as an adjuvant, its efficacy is substantially increased and side effects may be lessened. In addition, lactobacilli with other probiotic organisms in ripened milk and yogurt aid development of immunity in the intestine mucus in humans by raising the number of immunoglobulin A ( IgA (+)) antiodies.

Gastroesophageal reflux disease (GERD) is a common condition associated with bile acid-induced oxidative stress and accumulation of reactive oxygen species (ROS) in esophageal tissues that cause inflammation and DNA damage.Bernard, J.N.; Chinnaiyan, V.; Almeda, J.; Catala-Valentin, A.; Andl, C.D. Lactobacillus sp. Facilitate the Repair of DNA Damage Caused by Bile-Induced Reactive Oxygen Species in Experimental Models of Gastroesophageal Reflux Disease. Antioxidants 2023, 12, 1314. https://doi.org/10.3390/antiox12071314 In an experimental model of GERD, ''Lactobacillus'' species ''( L. acidophilus'', '' L. plantarum'', and '' L. fermentum'') facilitated the repair of DNA damage caused by bile-induced ROS. For patients with GERD, there is significant interest in the anti-inflammatory effect of lactobacilli that may help prevent progression to Barrett’s esophagus and esophageal adenocarcinoma.

Given the known microbial associations, lactobacilli are currently available as probiotics to help control urogenital and vaginal infections, such as bacterial vaginosis (BV). Lactobacilli produce bacteriocins to suppress the pathogenic growth of certain bacteria, as well as lactic acid, which lowers the vaginal pH to around 4.5 or less, hampering the survival of other bacteria.

In children, lactobacilli such as ''Lacticaseibacillus rhamnosus'' (previously '' L. rhamnosus'') are associated with a reduction of atopic eczema, also known as dermatitis, due to anti-inflammatory cytokines secreted by this probiotic bacteria.

Oral health

Some lactobacilli have been associated with cases of dental caries (cavities). Lactic acid can corrode teeth, and the ''Lactobacillus'' count in saliva has been used as a "caries test" for many years. Lactobacilli characteristically cause existing carious lesions to progress, especially those in coronal caries. The issue is, however, complex, as recent studies show probiotics can allow beneficial lactobacilli to populate sites on teeth, preventing streptococcal pathogens from taking hold and inducing dental decay. The scientific research of lactobacilli in relation to oral health is a new field and only a few studies and results have been published. Some studies have provided evidence of certain lactobacilli which can be a probiotic for oral health. Some species, but not all, show evidence in defense to dental caries. Due to these studies, there have been applications of incorporating such probiotics in chewing gum and lozenges. There is also evidence of certain lactobacilli that are beneficial in the defense of periodontal disease such as gingivitis and periodontitis.

Food production

Species of ''Lactobacillus'' (and related genera) comprise many food fermenting lactic acid bacteria and are used as starter cultures in industry for controlled fermentation in the production of wine, yogurt, cheese, sauerkraut, pickles, beer, cider, kimchi, cocoa, kefir, and other fermented foods, as well as animal feeds and the bokashi soil amendment. ''Lactobacillus'' species are dominant in yogurt, cheese, and sourdough fermentations.

Their importance in fermentation comes from both metabolism of the food itself, as well as the inhibition of growth of other potentially pathogenic microbes. The antibacterial and antifungal activity of lactobacilli relies on production of bacteriocins and low molecular weight compounds that inhibit these microorganisms.

Sourdough bread is made either spontaneously, by taking advantage of the bacteria naturally present in flour, or by using a "starter culture", which is a symbiotic culture of yeast and lactic acid bacteria growing in a water and flour medium. The bacteria metabolize sugars into lactic acid, which lowers the pH of their environment and creates the signature sourness associated with yogurt, sauerkraut, etc.

In many traditional pickling processes, vegetables are submerged in brine, and salt-tolerant lactobacilli feed on natural sugars found in the vegetables. The resulting mix of salt and lactic acid is a hostile environment for other microbes, such as fungi, and the vegetables are thus preserved, remaining edible for long periods.

Lactobacilli, especially ''Pediococcus'' and '' L. brevis'', are some of the most common beer spoilage organisms. They are, however, essential to the production of sour beers such as Belgian lambics and American wild ales, giving the beer a distinct tart flavor.

Scientist Elie Metchnikoff won a Nobel prize in 1908 for his work on LAB, the connection to food, and possible usage as a probiotic.'Lactic Acid Bacteria and Their Uses in Animal Feeding to Improve Food Safety' in Advances in Food and Nutrition Research, Volume 50 (Elsevier),

See also

* Lactobacillus L. anticaries
* Lactic acid fermentation
* MRS agar
* '' Pediococcus''
* Probiotics
* Proteobiotics
* Carbon monoxide-releasing molecules

References

External links

*

Lactobacillus at Milk the Funk Wiki

''Lactobacillus'' at Bac''Dive'' - the Bacterial Diversity Metadatabase

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Lactobacillaceae
Food microbiology
Gut flora bacteria
Garde manger
Gram-positive bacteria
Bacteria genera