Iodine-125 (
125I) is a
radioisotope
A radionuclide (radioactive nuclide, radioisotope or radioactive isotope) is a nuclide that has excess nuclear energy, making it unstable. This excess energy can be used in one of three ways: emitted from the nucleus as gamma radiation; transferr ...
of
iodine
Iodine is a chemical element with the symbol I and atomic number 53. The heaviest of the stable halogens, it exists as a semi-lustrous, non-metallic solid at standard conditions that melts to form a deep violet liquid at , and boils to a vi ...
which has uses in
biological assay
An assay is an investigative (analytic) procedure in laboratory medicine, mining, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence, amount, or functional activity of a ...
s,
nuclear medicine
Nuclear medicine or nucleology is a medical specialty involving the application of radioactive substances in the diagnosis and treatment of disease. Nuclear imaging, in a sense, is " radiology done inside out" because it records radiation emi ...
imaging and in
radiation therapy
Radiation therapy or radiotherapy, often abbreviated RT, RTx, or XRT, is a therapy using ionizing radiation, generally provided as part of cancer treatment to control or kill malignant cells and normally delivered by a linear accelerator. Radi ...
as brachytherapy to treat a number of conditions, including
prostate cancer,
uveal melanoma
Uveal melanoma is a type of eye cancer in the uvea of the eye. It is traditionally classed as originating in the Iris (anatomy), iris, choroid, and ciliary body, but can also be divided into class I (low metastatic risk) and class II (high metasta ...
s, and
brain tumor
A brain tumor occurs when abnormal cells form within the brain. There are two main types of tumors: malignant tumors and benign (non-cancerous) tumors. These can be further classified as primary tumors, which start within the brain, and seconda ...
s. It is the second longest-lived radioisotope of iodine, after
iodine-129
Iodine-129 (129I) is a long-lived radioisotope of iodine which occurs naturally, but also is of special interest in the monitoring and effects of man-made nuclear fission products, where it serves as both tracer and potential radiological contamin ...
.
Its
half-life
Half-life (symbol ) is the time required for a quantity (of substance) to reduce to half of its initial value. The term is commonly used in nuclear physics to describe how quickly unstable atoms undergo radioactive decay or how long stable at ...
is 59.49 days and it decays by
electron capture
Electron capture (K-electron capture, also K-capture, or L-electron capture, L-capture) is a process in which the proton-rich nucleus of an electrically neutral atom absorbs an inner atomic electron, usually from the K or L electron shells. Thi ...
to an excited state of
tellurium-125
There are 39 known isotopes and 17 nuclear isomers of tellurium (52Te), with atomic masses that range from 104 to 142. These are listed in the table below.
Naturally-occurring tellurium on Earth consists of eight isotopes. Two of these have been ...
. This state is not the metastable
125mTe, but rather a lower energy state that decays immediately by
gamma decay
A gamma ray, also known as gamma radiation (symbol γ or \gamma), is a penetrating form of electromagnetic radiation arising from the radioactive decay of atomic nuclei. It consists of the shortest wavelength electromagnetic waves, typically sh ...
with a maximum energy of 35
keV Kev can refer to:
Given name
* Kev Adams, French comedian, actor, screenwriter and film producer born Kevin Smadja in 1991
* Kevin Kev Carmody (born 1946), Indigenous Australian singer-songwriter
* Kev Coghlan (born 1988), Scottish Grand Prix moto ...
. Some of the excess energy of the excited
125Te may be
internally converted ejected electrons (also at 35 keV), or to
x-rays
An X-ray, or, much less commonly, X-radiation, is a penetrating form of high-energy electromagnetic radiation. Most X-rays have a wavelength ranging from 10 picometers to 10 nanometers, corresponding to frequencies in the range 30&nbs ...
(from electron
bremsstrahlung
''Bremsstrahlung'' (), from "to brake" and "radiation"; i.e., "braking radiation" or "deceleration radiation", is electromagnetic radiation produced by the deceleration of a charged particle when deflected by another charged particle, typicall ...
), and also a total of 21
Auger electrons, which are produced at the low energies of 50 to 500 electron volts. Eventually, stable ground state
125Te is produced as the final decay product.
In medical applications, the internal conversion and Auger electrons cause little damage outside the cell which contains the isotope atom. The X-rays and gamma rays are of low enough energy to deliver a higher radiation dose selectively to nearby tissues, in "permanent" brachytherapy where the isotope capsules are left in place (
125I competes with
palladium-103 in such uses).
Because of its relatively long half-life and emission of low-energy photons which can be detected by
gamma-counter crystal detectors,
125I is a preferred isotope for
tagging antibodies
An antibody (Ab), also known as an immunoglobulin (Ig), is a large, Y-shaped protein used by the immune system to identify and neutralize foreign objects such as pathogenic bacteria and viruses. The antibody recognizes a unique molecule of the ...
in
radioimmunoassay
A radioimmunoassay (RIA) is an immunoassay that uses radiolabeled molecules in a stepwise formation of immune complexes. A RIA is a very sensitive in vitro assay technique used to measure concentrations of substances, usually measuring antigen conc ...
and other gamma-counting procedures involving
protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...
s outside the body. The same properties of the isotope make it useful for brachytherapy, and for certain nuclear medicine scanning procedures, in which it is attached to proteins (
albumin
Albumin is a family of globular proteins, the most common of which are the serum albumins. All the proteins of the albumin family are water-soluble, moderately soluble in concentrated salt solutions, and experience heat denaturation. Albumins ...
or
fibrinogen
Fibrinogen (factor I) is a glycoprotein complex, produced in the liver, that circulates in the blood of all vertebrates. During tissue and vascular injury, it is converted enzymatically by thrombin to fibrin and then to a fibrin-based blood cl ...
), and where a half-life longer than that provided by
123I is required for diagnostic or lab tests lasting several days.
Iodine-125 can be used in
scanning/imaging the
thyroid
The thyroid, or thyroid gland, is an endocrine gland in vertebrates. In humans it is in the neck and consists of two connected lobes. The lower two thirds of the lobes are connected by a thin band of tissue called the thyroid isthmus. The thy ...
, but iodine-123 is preferred for this purpose, due to better
radiation penetration and shorter half-life (13 hours).
125I is useful for
glomerular filtration rate
Renal functions include maintaining an acid–base balance; regulating fluid balance; regulating sodium, potassium, and other electrolytes; clearing toxins; absorption of glucose, amino acids, and other small molecules; regulation of blood p ...
(GFR) testing in the diagnosis or monitoring of patients with
kidney disease
Kidney disease, or renal disease, technically referred to as nephropathy, is damage to or disease of a kidney. Nephritis is an inflammatory kidney disease and has several types according to the location of the inflammation. Inflammation can ...
. Iodine-125 is used ''therapeutically'' in
brachytherapy
Brachytherapy is a form of radiation therapy where a sealed radiation source is placed inside or next to the area requiring treatment. ''Brachy'' is Greek for short. Brachytherapy is commonly used as an effective treatment for cervical, prost ...
treatments of
tumor
A neoplasm () is a type of abnormal and excessive growth of tissue. The process that occurs to form or produce a neoplasm is called neoplasia. The growth of a neoplasm is uncoordinated with that of the normal surrounding tissue, and persists ...
s. For
radiotherapy ablation
Ablation ( la, ablatio – removal) is removal or destruction of something from an object by vaporization, chipping, erosion, erosive processes or by other means. Examples of ablative materials are described below, and include spacecraft materi ...
of tissues that absorb iodine (such as the thyroid), or that absorb an iodine-containing
radiopharmaceutical
Radiopharmaceuticals, or medicinal radiocompounds, are a group of pharmaceutical drugs containing radioactive isotopes. Radiopharmaceuticals can be used as diagnostic and therapeutic agents. Radiopharmaceuticals emit radiation themselves, which is ...
, the
beta-emitter iodine-131
Iodine-131 (131I, I-131) is an important radioisotope of iodine discovered by Glenn Seaborg and John Livingood in 1938 at the University of California, Berkeley. It has a radioactive decay half-life of about eight days. It is associated with nu ...
is the preferred isotope.
When studying
plant immunity, I is used as the
radiolabel
A radioactive tracer, radiotracer, or radioactive label is a chemical compound in which one or more atoms have been replaced by a radionuclide so by virtue of its radioactive decay it can be used to explore the mechanism of chemical reactions by tr ...
in tracking
ligand
In coordination chemistry, a ligand is an ion or molecule (functional group) that binds to a central metal atom to form a coordination complex. The bonding with the metal generally involves formal donation of one or more of the ligand's electr ...
s to determine which
plant pattern recognition receptor
Pattern recognition receptors (PRRs) play a crucial role in the proper function of the innate immune system. PRRs are germline-encoded host sensors, which detect molecules typical for the pathogens. They are proteins expressed, mainly, by cells of ...
s (PRRs) they bind to.
125I is produced by the
electron capture
Electron capture (K-electron capture, also K-capture, or L-electron capture, L-capture) is a process in which the proton-rich nucleus of an electrically neutral atom absorbs an inner atomic electron, usually from the K or L electron shells. Thi ...
decay of
125Xe, which is an artificial isotope of
xenon
Xenon is a chemical element with the symbol Xe and atomic number 54. It is a dense, colorless, odorless noble gas found in Earth's atmosphere in trace amounts. Although generally unreactive, it can undergo a few chemical reactions such as the ...
, itself created by
neutron capture
Neutron capture is a nuclear reaction in which an atomic nucleus and one or more neutrons collide and merge to form a heavier nucleus. Since neutrons have no electric charge, they can enter a nucleus more easily than positively charged protons, ...
of near-stable
124Xe (it undergoes
double electron capture
Double electron capture is a decay mode of an atomic nucleus. For a nuclide (''A'', ''Z'') with a number of nucleons ''A'' and atomic number ''Z'', double electron capture is only possible if the mass of the nuclide (''A'', ''Z''−2) is lower.
...
with a half life orders of magnitude larger than the age of the universe), which makes up around 0.1% of naturally occurring xenon. Because of the artificial production route of
125I and its short half-life, its
natural abundance
In physics, natural abundance (NA) refers to the abundance of isotopes of a chemical element as naturally found on a planet. The relative atomic mass (a weighted average, weighted by mole-fraction abundance figures) of these isotopes is the atomi ...
on Earth is effectively zero.
Production
125I is a reactor-produced radionuclide and is available in large quantities. Its production follows the two reactions:
124Xe (n,γ) →
125mXe (57 s) →
125I (59.4 d)
124Xe (n,γ) →
125gXe (19.9 h) →
125I (59.4 d)
The irradiation target is natural
xenon
Xenon is a chemical element with the symbol Xe and atomic number 54. It is a dense, colorless, odorless noble gas found in Earth's atmosphere in trace amounts. Although generally unreactive, it can undergo a few chemical reactions such as the ...
gas
Gas is one of the four fundamental states of matter (the others being solid, liquid, and plasma).
A pure gas may be made up of individual atoms (e.g. a noble gas like neon), elemental molecules made from one type of atom (e.g. oxygen), or ...
containing 0.0965 atom % (
mole fraction
In chemistry, the mole fraction or molar fraction (''xi'' or ) is defined as unit of the amount of a constituent (expressed in moles), ''ni'', divided by the total amount of all constituents in a mixture (also expressed in moles), ''n''tot. This ex ...
) of the
primordial nuclide 124Xe, which is the target isotope for making
125I by
neutron capture
Neutron capture is a nuclear reaction in which an atomic nucleus and one or more neutrons collide and merge to form a heavier nucleus. Since neutrons have no electric charge, they can enter a nucleus more easily than positively charged protons, ...
. It is loaded into irradiation capsules of the zirconium alloy
zircaloy-2 (a corrosion resisting alloy transparent to
neutron
The neutron is a subatomic particle, symbol or , which has a neutral (not positive or negative) charge, and a mass slightly greater than that of a proton. Protons and neutrons constitute the nuclei of atoms. Since protons and neutrons beh ...
s) to a pressure of about 100
bar
Bar or BAR may refer to:
Food and drink
* Bar (establishment), selling alcoholic beverages
* Candy bar
* Chocolate bar
Science and technology
* Bar (river morphology), a deposit of sediment
* Bar (tropical cyclone), a layer of cloud
* Bar (u ...
(about 100
atm). Upon irradiation with
slow neutrons in a
nuclear reactor
A nuclear reactor is a device used to initiate and control a fission nuclear chain reaction or nuclear fusion reactions. Nuclear reactors are used at nuclear power plants for electricity generation and in nuclear marine propulsion. Heat fr ...
, several
radioisotopes of xenon are produced. However, only the decay of
125Xe leads to a radioiodine:
125I. The other xenon radioisotopes decay either to stable
xenon
Xenon is a chemical element with the symbol Xe and atomic number 54. It is a dense, colorless, odorless noble gas found in Earth's atmosphere in trace amounts. Although generally unreactive, it can undergo a few chemical reactions such as the ...
, or to various
caesium isotopes, some of them radioactive (a.o., the long-lived
135Cs and
137Cs).
Long
irradiation
Irradiation is the process by which an object is exposed to radiation. The exposure can originate from various sources, including natural sources. Most frequently the term refers to ionizing radiation, and to a level of radiation that will serve ...
times are disadvantageous. Iodine-125 itself has a
neutron capture
Neutron capture is a nuclear reaction in which an atomic nucleus and one or more neutrons collide and merge to form a heavier nucleus. Since neutrons have no electric charge, they can enter a nucleus more easily than positively charged protons, ...
cross section
Cross section may refer to:
* Cross section (geometry)
** Cross-sectional views in architecture & engineering 3D
*Cross section (geology)
* Cross section (electronics)
* Radar cross section, measure of detectability
* Cross section (physics)
**Abs ...
of 900
barns
A barn is an agricultural building usually on farms and used for various purposes. In North America, a barn refers to structures that house livestock, including cattle and horses, as well as equipment and fodder, and often grain.Allen G. ...
, and consequently during a long irradiation, part of the
125I formed will be converted to
126I, a
beta-emitter and
positron-emitter with a
half-life
Half-life (symbol ) is the time required for a quantity (of substance) to reduce to half of its initial value. The term is commonly used in nuclear physics to describe how quickly unstable atoms undergo radioactive decay or how long stable at ...
of 13.1 days, which is not medically useful. In practice, the most useful irradiation time in the reactor amounts to a few days. Thereafter, the irradiated gas is allowed to decay for three or four days to eliminate short-lived unwanted radioisotopes, and to allow the newly created xenon-125 (half-life 17 hours) to decay to iodine-125.
To isolate radioiodine, the irradiated capsule is first cooled at low temperature (to collect free iodine gas on the capsule inner wall) and the remaining Xe gas is vented in a controlled way and recovered for further use. The inner walls of the capsule are then rinsed with dilute NaOH solution to collect iodine as soluble
iodide
An iodide ion is the ion I−. Compounds with iodine in formal oxidation state −1 are called iodides. In everyday life, iodide is most commonly encountered as a component of iodized salt, which many governments mandate. Worldwide, iodine de ...
(I
−) and
hypoiodite (IO
−), according to the standard
disproportionation
In chemistry, disproportionation, sometimes called dismutation, is a redox reaction in which one compound of intermediate oxidation state converts to two compounds, one of higher and one of lower oxidation states. More generally, the term can ...
reaction of
halogen
The halogens () are a group in the periodic table consisting of five or six chemically related elements: fluorine (F), chlorine (Cl), bromine (Br), iodine (I), astatine (At), and tennessine (Ts). In the modern IUPAC nomenclature, this group is ...
s in alkaline solutions. Any
caesium
Caesium (IUPAC spelling) (or cesium in American English) is a chemical element with the symbol Cs and atomic number 55. It is a soft, silvery-golden alkali metal with a melting point of , which makes it one of only five elemental metals that a ...
atom present immediately
oxidizes and passes into the water as Cs
+. In order to eliminate any long-lived
135Cs and
137Cs which may be present in small amounts, the solution is passed through a
cation-exchange
Ion exchange is a reversible interchange of one kind of ion present in an insoluble solid with another of like charge present in a solution surrounding the solid with the reaction being used especially for softening or making water demineralised, ...
column, which exchanges Cs
+ for another non-radioactive cation. The radioiodine (as anion I
− or IO
−) remains in solution as iodide/hypoiodite.
Availability and purity
Iodine-125 is commercially available in dilute
NaOH
Sodium hydroxide, also known as lye and caustic soda, is an inorganic compound with the formula NaOH. It is a white solid ionic compound consisting of sodium cations and hydroxide anions .
Sodium hydroxide is a highly caustic base and alkali ...
solution as
125I-iodide (or the
hypohalite sodium
hypoiodite, NaIO). The radioactive concentration lies at 4 to 11 GBq/ml and the specific radioactivity is >75 GBq/µmol (7.5 × 10
16 Bq/mol). The chemical and radiochemical purity is high. The radionuclidic purity is also high; some
126I (t
1/2 = 13.1 d) is unavoidable due to the
neutron capture
Neutron capture is a nuclear reaction in which an atomic nucleus and one or more neutrons collide and merge to form a heavier nucleus. Since neutrons have no electric charge, they can enter a nucleus more easily than positively charged protons, ...
noted above. The
126I tolerable content (which is set by the unwanted isotope interfering with
dose calculations in brachytherapy) lies at about 0.2 atom % (atom fraction) of the total iodine (the rest being
125I).
Producers
As of October 2019, there were two producers of iodine-125, the
McMaster Nuclear Reactor
The McMaster Nuclear Reactor (MNR) is a 5 MWth open pool reactor located on the campus of McMaster University, in Hamilton, Ontario, Canada.
Description
MNR began operating in April 1959, as the first university-based research reactor in t ...
in
Hamilton Hamilton may refer to:
People
* Hamilton (name), a common British surname and occasional given name, usually of Scottish origin, including a list of persons with the surname
** The Duke of Hamilton, the premier peer of Scotland
** Lord Hamilt ...
,
Ontario
Ontario ( ; ) is one of the thirteen provinces and territories of Canada.Ontario is located in the geographic eastern half of Canada, but it has historically and politically been considered to be part of Central Canada. Located in Central Ca ...
, Canada; and a research reactor in Uzbekistan.
The McMaster reactor is presently the largest producer of iodine-125, producing approximately 60 per cent of the global supply in 2018;
with the remaining global supply produced at the reactor based in Uzbekistan. Annually, the McMaster reactor produces enough iodine-125 to treat approximately 70,000 patients.
In November 2019, the research reactor in Uzbekistan shut down temporarily in order to facilitate repairs. The temporary shutdown threatened the global supply of the radioisotope by leaving the McMaster reactor as the sole producer of iodine-125 during the period.
[
Prior to 2018, the National Research Universal (NRU) reactor at ]Chalk River Laboratories
Chalk River Laboratories (french: Laboratoires de Chalk River; also known as CRL, Chalk River Labs and formerly Chalk River Nuclear Laboratories, CRNL) is a Canadian nuclear research facility in Deep River, about north-west of Ottawa.
CRL is a ...
in Deep River, Ontario, was one of three reactors to produce iodine-125. However, on March 31, 2018, the NRU reactor was permanently shut down ahead of its scheduled decommissioning in 2028, as a result of a government order. The Russian nuclear reactor equipped to produce iodine-125, was offline as of December 2019.[
]
Decay properties
The detailed decay mechanism to form the stable daughter nuclide tellurium-125 is a multi-step process that begins with electron capture
Electron capture (K-electron capture, also K-capture, or L-electron capture, L-capture) is a process in which the proton-rich nucleus of an electrically neutral atom absorbs an inner atomic electron, usually from the K or L electron shells. Thi ...
. This is followed by a cascade of electron relaxation as the core electron hole moves toward the valence orbital
In chemistry and physics, a valence electron is an electron in the outer shell associated with an atom, and that can participate in the formation of a chemical bond if the outer shell is not closed. In a single covalent bond, a shared pair forms ...
s. The cascade involves many Auger transitions, each of which cause the atom to become increasingly ionized
Ionization, or Ionisation is the process by which an atom or a molecule acquires a negative or positive charge by gaining or losing electrons, often in conjunction with other chemical changes. The resulting electrically charged atom or molecule ...
. The electron capture produces a tellurium-125 nucleus in an excited state with a half-life of 1.6 ns, which undergoes gamma decay
A gamma ray, also known as gamma radiation (symbol γ or \gamma), is a penetrating form of electromagnetic radiation arising from the radioactive decay of atomic nuclei. It consists of the shortest wavelength electromagnetic waves, typically sh ...
emitting a gamma photon
A photon () is an elementary particle that is a quantum of the electromagnetic field, including electromagnetic radiation such as light and radio waves, and the force carrier for the electromagnetic force. Photons are massless, so they always ...
or an internal conversion
Internal conversion is a non-radioactive, atomic decay process where an excited nucleus interacts electromagnetically with one of the orbital electrons of an atom. This causes the electron to be emitted (ejected) from the atom. Thus, in internal ...
electron
The electron ( or ) is a subatomic particle with a negative one elementary electric charge. Electrons belong to the first generation of the lepton particle family,
and are generally thought to be elementary particles because they have no kn ...
at 35.5 keV. A second electron relaxation cascade follows the gamma decay before the nuclide comes to rest. Throughout the entire process an average of 13.3 electrons are emitted (10.3 of which are Auger electron
The Auger effect or Auger−Meitner effect is a physical phenomenon in which the filling of an inner-shell vacancy of an atom is accompanied by the emission of an electron from the same atom. When a core electron is removed, leaving a vacancy, ...
s), most with energies less than 400 eV (79% of yield). The internal conversion and Auger electrons from the radioisotope have been found in one study to do little cellular damage, unless the radionuclide is directly incorporated chemically into cellular DNA, which is not the case for present radiopharmaceuticals which use 125I as the radioactive label nuclide.
As with other radioisotopes of iodine, accidental iodine-125 uptake in the body (mostly by the thyroid
The thyroid, or thyroid gland, is an endocrine gland in vertebrates. In humans it is in the neck and consists of two connected lobes. The lower two thirds of the lobes are connected by a thin band of tissue called the thyroid isthmus. The thy ...
gland) can be blocked by the prompt administration of stable iodine-127
There are 37 known isotopes of iodine (53I) from 108I to 144I; all undergo radioactive decay except 127I, which is stable. Iodine is thus a monoisotopic element.
Its longest-lived radioactive isotope, 129I, has a half-life of 15.7 million yea ...
in the form of an iodide salt.[Michigan State University (October 2013). Radiation safety manual, Environmental Health & Safety, see I-125, p. 81.](_blank)
/ref> Potassium iodide
Potassium iodide is a chemical compound, medication, and dietary supplement. It is a medication used for treating hyperthyroidism, in radiation emergencies, and for protecting the thyroid gland when certain types of radiopharmaceuticals are u ...
(KI) is typically used for this purpose.
However, unjustified self-medicated preventive administration of stable KI is not recommended in order to avoid to disturb the normal thyroid function. Such a treatment must be carefully dosed and requires an appropriate KI amount prescribed by a specialised physician.
See also
* Isotopes of iodine
There are 37 known isotopes of iodine (53I) from 108I to 144I; all undergo radioactive decay except 127I, which is stable. Iodine is thus a monoisotopic element.
Its longest-lived radioactive isotope, 129I, has a half-life of 15.7 million yea ...
* Iodine-123
Iodine-123 (123I) is a radioactive isotope of iodine used in nuclear medicine imaging, including single-photon emission computed tomography (SPECT) or SPECT/CT exams. The isotope's half-life is 13.2230 hours; the decay by electron capture to tellu ...
* Iodine-129
Iodine-129 (129I) is a long-lived radioisotope of iodine which occurs naturally, but also is of special interest in the monitoring and effects of man-made nuclear fission products, where it serves as both tracer and potential radiological contamin ...
* Iodine-131
Iodine-131 (131I, I-131) is an important radioisotope of iodine discovered by Glenn Seaborg and John Livingood in 1938 at the University of California, Berkeley. It has a radioactive decay half-life of about eight days. It is associated with nu ...
* Iodine in biology
Iodine is an essential trace element in biological systems. It has the distinction of being the heaviest element commonly needed by living organisms as well as the second-heaviest known to be used by any form of life (only tungsten, a component ...
** 2,5-Dimethoxy-4-iodoamphetamine (DOI), often labeled with 125I
Notes and references
{{Radiopharmaceuticals
Isotopes of iodine
Radiopharmaceuticals
Radiobiology
Radiation therapy