Heat stabilization is an additive-free preservation technology for tissue samples which stops degradation and changes immediately and permanently. Heat stabilization uses rapid conductive heating, under controlled pressure, to generate a fast, homogeneous and irreversible thermal denaturation of proteins, resulting in a complete and permanent elimination of all enzymatic activity that would otherwise cause further biological changes to the tissue sample ''ex vivo''. Due to the permanent inactivation of enzymes, heat stabilization overcomes the drawbacks of conventional tissue sample preservation techniques, such as snap-freezing followed by inhibitors. Understanding the role of

proteins Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, respo ...

peptides Peptides (, ) are short chains of amino acids linked by peptide bonds. Long chains of amino acids are called proteins. Chains of fewer than twenty amino acids are called oligopeptides, and include dipeptides, tripeptides, and tetrapeptides. A p ...

and

small molecules Within the fields of molecular biology and pharmacology, a small molecule or micromolecule is a low molecular weight (≤ 1000 daltons) organic compound that may regulate a biological process, with a size on the order of 1 nm. Many drugs are ...

in normal and diseased tissue is crucial to defining their potential use as drugs, drug targets or disease

biomarkers In biomedical contexts, a biomarker, or biological marker, is a measurable indicator of some biological state or condition. Biomarkers are often measured and evaluated using blood, urine, or soft tissues to examine normal biological processes, p ...

. Yet biological changes begin the moment tissue is removed from its native environment. Dramatic alterations at the molecular level occur within seconds e.g. changed metabolism, catabolic fragmentation of large molecules (such as ATP) occurs in order to release energy, leading to disrupted control mechanisms,

phosphorylation In chemistry, phosphorylation is the attachment of a phosphate group to a molecule or an ion. This process and its inverse, dephosphorylation, are common in biology and could be driven by natural selection. Text was copied from this source, wh ...

states are altered and proteins begin to degrade. As a consequence vital information may be lost or distorted, leading to inter-sample variation, risk of incorrect data interpretation and potentially misleading conclusions. Heat stabilization offers significant advantages over conventional approaches to preventing biological change. It can be used to replace snap freezing followed by inhibitors, pH changes, organic solvents or cross-linking. It can also be used with frozen tissue, allowing stabilization of stored samples. Heat stabilization can be used for almost any kind of tissue sample, and has been verified to be compatible with many downstream analytical techniques such as

mass spectrometry Mass spectrometry (MS) is an analytical technique that is used to measure the mass-to-charge ratio of ions. The results are presented as a ''mass spectrum'', a plot of intensity as a function of the mass-to-charge ratio. Mass spectrometry is use ...

, phospho-shotgun,

MALDI imaging MALDI mass spectrometry imaging (MALDI-MSI) is the use of matrix-assisted laser desorption ionization as a mass spectrometry imaging technique in which the sample, often a thin tissue section, is moved in two dimensions while the mass spectrum is ...

Western blot The western blot (sometimes called the protein immunoblot), or western blotting, is a widely used analytical technique in molecular biology and immunogenetics to detect specific proteins in a sample of tissue homogenate or extract. Besides detect ...

, 1D and 2D gels, reversed-phased protein arrays,

RIA A ria (; gl, ría) is a coastal inlet formed by the partial submergence of an unglaciated river valley. It is a drowned river valley that remains open to the sea. Definitions Typically rias have a dendritic, treelike outline although they ca ...

and

ELISA The enzyme-linked immunosorbent assay (ELISA) (, ) is a commonly used analytical biochemistry assay, first described by Eva Engvall and Peter Perlmann in 1971. The assay uses a solid-phase type of enzyme immunoassay (EIA) to detect the presence ...

. The method also allows samples collected and handled in bio safety level laboratories to be subsequently handled outside such labs after treatment.

References

{{Reflist Scientific techniques Proteomics