Aphanoascus Fulvescens

''Aphanoascus fulvescens'' is a mould fungus that behaves as a keratinophilic saprotroph and belongs to the Ascomycota. It is readily isolated from soil and dung containing keratin-rich tissues that have been separated from their animal hosts. This organism, distributed worldwide, is most commonly found in areas of temperate climate, in keeping with its optimal growth temperature of . While ''A. fulvescens'' is recognized as a geophilic fungal species, it is also a facultative opportunistic pathogen. Although it is not a dermatophyte, ''A. fulvescens'' has occasionally been shown to cause onychomycosis infections in humans. Its recognition in the laboratory is clinically important for correct diagnosis and treatment of human dermal infections.

Taxonomy

''Aphanoascus fulvescens'' has faced many discrepancies and challenges for proper taxonomic placement. The naming and classification of ''A. fulvescens'' has been subject to taxonomic confusion since the original discovery of the species.Cano J, Guarro J. (April 1990). “The genus ''Aphanoascus''”. ''Mycological Research'' 94: 355–377 The genus ''Aphanoascus'', to which ''A. fulvescens'' belongs, was first described in 1890 by Hugo Zukal after isolating it from alligator dung in Vienna.Rippon JW, Lee FC, McMillen S. (1970). “Dermatophyte infection caused by ''Aphanoascus fulvescens''”. ''Archives of Dermatology'' 102(5):552-&. doi:10.1001/archderm.102.5.552 Zukal named this fungus ''Aphanoascus cinnabarinus''. The same fungus however, had already been isolated earlier in 1875 under the name ''Badhamia fulvescens'' by Cooke. The genus ''Aphanoascus'' was then further reviewed by Apinis in 1968 who deemed ''A. fulvescens'' as the type species. While most mycologists accept this title, others argue that ''Aphanoascus'' is a synonym for ''Anixiopsis'', which was the genus name given to a soil isolate described by Hansen as ''Anixiopsis fulvescens''. Later in 1973, further confusion was caused with the introduction of ''Aphanoascus cinnabarinus'' as the new type species for the genus ''Aphanoascus'' by Udagawa & Takada. In 1980, Benny & Kimbrough recognized ''Aphanoascus'' and ''Anixiopsis'' as two distinct genera, the former distinguished by reticulate ascospores and the latter by ridged ascospores. Whether the two genera are synonymous remains controversial; many authors continue to accept them as one genus.
Initially, ''A. fulvescens'' was placed in the family Cephalothecaceae by Apinis, in the Onygenaceae by Malloch & Cain and Currah, in Trichocomaceae by Benny & Kimbrough, and in Amauroascaceae by Arx.
Today, most mycologists accept ''A. fulvescens'' as the type species of the genus ''Aphanoascus'' under the family Onygenaceae.
''A. fulvescens'' refers to the sexual state, or teleomorph, of the organism. ''Chrysosporium keratinophilum'' is regarded by some mycologists as the asexual state, or anamorph, however others argue that this is only the asexual state of ''Aphanoascus keratinophilus''.Campbell, C. K., Johnson, E. M. and Warnock, D. W. (2013) "Miscellaneous Moulds".''Identification of Pathogenic Fungi Second Edition''. Wiley-Blackwell. Oxford, UK. ''Aphanoascus fulvescens'', ''Aphanoascus terreus'', ''Aphanoascus canadensis'', ''Aphanoascus reticulisporus'', and ''Keratinophyton durum'' or ''Anixiopsis biplanata'' are the species that are currently accepted under the genus ''Aphanoascus''.

Appearance

The colonies of ''Aphanoascus fulvescens'' at on glucose-peptone agar have a flat topology and a powdery to felt-like texture and are usually white to cream coloured depending on the number of brown ascomata present on the surface. Under the microscope, ''A. fulvescens'' is seen to have numerous, large club-shaped conidia with older colonies containing fruiting bodies, or ascomata, at their centres. These fruiting bodies are relatively large, ranging from 290–500 μm in diameter, and are either colourless or have a light brown pigment. The presence of the ascomata is characteristic of all members of the Ascomycota. The ascomata are smooth and have thick, enclosed walls lacking any openings. They reach full maturity after 8–10 days of colonial growth at optimal temperatures and eventually burst to release the asci contained within them. The asci have walls composed of two to four layers of flattened cells which in total amount to 4–6 μm in thickness. There are eight ascospores contained within each ascus in ''A. fulvescens'' colonies. The ellipsoidal ascospores are a light brown colour and are either lens shaped or disc shaped. They can be as large as 5 x 3.5 μm and have notably rough walls. The ascospores of ''A. fulvescens'' are different than those of other members of the Amauroascaeae which have ascospores that are usually globular and round. Rather, the unicellular ascospores of ''A. fulvescens'' are found to be flattened with reticulate walls. The hyphae of ''A. fulvescens'' are hyaline, branched, and contain many cross-walls (setpa). They are generally 1.7–3 μm wide and are contained within thin walls.
The colonial appearance of ''A. fulvescens'' generally mimics that of species in the genus ''Trichophyton'' and ''Chrysosporium'' while under the microscope the appearance of the organism resembles that of the genus '' Aspergillus''. The differentiation of ''A. fulvescens'' from Aspergillus spp. is based on their conidial states.

Ecology

''Aphanoascus fulvescens'' is commonly found in the soil and in dung, living as a keratinophilic saprotroph. It is also often isolated from keratin-rich tissues such as hair and nails that have been discarded from the host. ''A. fulvescens'' is a geophilic fungus and therefore does not normally infect mammals. The organism is more commonly isolated from soil that has been inhabited frequently by animals than soil that has not. However, while this fungus is not a true dermatophyte, it is opportunistic and has been seen to cause dermatophytosis in humans and other mammals on occasion.Marin G, Campos R. (1984). “Dermatophytosis due to ''Aphanoascus fulvescens''”. ''Sabouraudia Journal of Medical and Veterinary Mycology'' 22(4):311–14. ''A. fulvescens'' has a worldwide distribution, however it is more frequently isolated in temperate climates. This is because the organism has optimal growth at 28 °C at which it grows 3–4 mm per day and up to 25 mm per week. At elevated temperatures, such as that of the human body (37 °C), ''A. fulvescens'' shows restricted growth, averaging only 5–6 mm of growth over the course of seven days. This may be a possible explanation as to why infections involving ''A. fulvescens'' are uncommon in humans and other mammals.

Pathogenicity

While ''Aphanoascus fulvescens'' is a geophilic fungus, it is also opportunistic and therefore has been isolated from humans and animals on occasion. In order to cause infection, the fungus must first adhere to the host cells. '' In vivo'' and '' in vitro'' experiments have proposed the possible role of secreted serine proteases in order to mediate this adhesion. Adhesion of ''A. fulvescens'' to the host cell is necessary, because the organism cannot degrade keratin from a distance and instead uses pressure to penetrate the keratin-rich cells of the host.Cano J, Guarro J, Mayayo J. (January 1990). “Experimental Pathogenicity of ''Aphanoascus'' spp.”. ''Mycoses'' 33(1): 41–45.

Skin

Keratinophilic fungal species that fall under the genus ''Trichophyton'', '' Epidermophyton'', or '' Microsporum'', are pathogenic to humans and other animals, and are referred to as dermatophytes on the basis of their ability to cause dermatophytosis in these hosts.de Vries G. (1962). “Keratinophilic fungi and their action”. ''Antonie van Leeuwenhoek'' 28:121–33 However, infections in these hosts caused by soil-derived keratinophilic saprotrophs like ''A. fulvescens'' are very seldom and only a few cases have been documented in the literature. The first documented case of ''A. fulvescens'' causing tinea corporis was in 1970 when the fungus was repeatedly isolated from a 4 x 5 cm red patch on the inner right thigh of a 21-year-old white male. This case showcases the clinical importance of non-dermatophyte keratinophilic fungi such as ''A. fulvescens'' as a result of the ability to cause dermatophytosis in the form of tinea corporis in humans. While visually, the case of tinea corporis was indistinguishable from those caused by true dermatophytes, the infection did not respond well to typical treatment. This observation is crucial for correct diagnosis and treatment of the skin lesion and highlights the necessity of awareness for atypical opportunistic human pathogenic fungi.

Hair

The cuticle is the first region of the hair that is attacked in an ''A. fulvescens'' infection.English MP. “Destruction of hair by ''Chrysosporium keratinophilum''”. (April 1969). ''Transactions of the British Mycological Society'' 52(2):247–255.Cano J. Guarro J, Figueras M. (March 1991). "Study of the invasion of human hair in vitro by ''Aphanoascus'' spp." ''Mycoses''. 34(3):145–152. This attack resembles that of true dermatophytes in that some lateral hyphae give rise to fronded mycelium. However, some hyphae also give rise to appressoria, or flattened hyphal pressing organs, which are characteristic of non-keratinophilic fungi. Dermatophytes digest the hair cuticle rapidly, unlike ''A. fulvescens'', which breaks the cuticle down so slowly that the scales are still visible for weeks after the hair has been infected.

After the cuticle has been broken down, the next structure that is targeted is the medulla. This region is first colonized by hyphae present in the cuticle and fungal growth is more rapid here because the trichohyalin of the medulla is more readily digested than the keratin of the cuticle. The medullary mycelium then grow back towards the cuticle in a manner that makes the origin of the mycelium undetectable after 5–6 days of infection.

The cortex is the third, and last region of the hair to be attacked by ''A. fulvescens''. Appressoria penetrate through to the cortex to produce cells of various sizes, giving the cortex a swollen morphology. This swollen mass of cells can proliferate in all directions to give rise to a large mass of mycelium that protrudes backwards out of the hair. Dermatophytes are seen to behave in a similar manner, however, instead of a bulging mass, the mycelium grows in a more organized fashion, as a single collapsed column.

Growth of the cortical mycelium can be at any angle to the hair in an attack from ''A. fulvescens'', while true dermatophytes have the characteristic of growing along the hair in parallel. ''A. fulvescens'' can attack at any point along the hair and often causes a marked swelling upon entry sites. The mycelium penetrates the hair by exerting a high amount of pressure on the hair cells and is always closely pressed to the cavity walls. ''A. fulvescens'' cannot digest keratin from a distance, as seen in dermatophytes, but rather seems to require direct contact with the keratin-rich substrate. The digestion of keratin by ''A. fulvescens'' happens in two stages, the first of which involves the denaturation of keratin. The second stage involves the actual digestion of keratin by ''A. fulvescens'', which then grows into the remaining space. An infected hair is completely replaced by the mycelium of ''A. fulvescens'' within 6–8 weeks.

Treatment

Topical steroids and antibiotic ointments such as Neosporin have not only shown to be ineffective in treating dermatophytosis caused by ''Aphanoascus fulvescens'', but have also been shown to enhance its fungal growth. Ringworm infections caused by ''A. fulvescens'' in animals have been successfully treated with anti-fungal drugs such as Tinactin, which contains tolnaftate as its active ingredient, and Grisactin, which contains griseofulvin as its active ingredient. However, topical treatment of human ''A. fulvescens'' infections with tolnaftate-containing drugs has been shown to worsen the lesions, causing further inflammation and increased sensitivity. Treatment with griseofulvin has proven somewhat effective in reducing lesion sizes and appears to have a dose-dependent effect. While lesions continue to heal after increased griseofulvin administration, there is some residual scarring of the infected area. Currently, there is no documentation providing evidence of an anti-fungal treatment that is completely effective against human dermatophytosis caused by ''A. fulvescens''.

References

{{Taxonbar, from=Q10414566

Fungi described in 1875
Onygenales