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Xanthoproteic Reaction
The xanthoproteic reaction is a method that can be used to detect a presence of protein soluble in a solution, using concentrated nitric acid. The test gives a positive result in amino acids carrying aromatic groups, especially in the presence of tyrosine. If the test is positive the proof is neutralized with an alkali, turning dark yellow. The yellow colour is due to xanthoproteic acid which is formed due to nitration of certain amino acids, most common examples being tyrosine and tryptophan. This chemical reaction is a qualitative test, determining the presence or absence of proteins. Procedure Add 1 ml of concentrated HNO3 to 1 ml of the test sample. Heat the mixture and cool it. Slowly add sodium hydroxide (40% w/v in water) solution until the mixture becomes alkaline and a colour change is noted. If the colour changes from yellow to orange, this indicates the presence of an aromatic amino acid. When human skin or nails are exposed to nitric acid, they turn yellow after ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Sodium Hydroxide
Sodium hydroxide, also known as lye and caustic soda, is an inorganic compound with the formula NaOH. It is a white solid ionic compound consisting of sodium cations and hydroxide anions . Sodium hydroxide is a highly caustic base and alkali that decomposes proteins at ordinary ambient temperatures and may cause severe chemical burns. It is highly soluble in water, and readily absorbs moisture and carbon dioxide from the air. It forms a series of hydrates . The monohydrate crystallizes from water solutions between 12.3 and 61.8 °C. The commercially available "sodium hydroxide" is often this monohydrate, and published data may refer to it instead of the anhydrous compound. As one of the simplest hydroxides, sodium hydroxide is frequently used alongside neutral water and acidic hydrochloric acid to demonstrate the pH scale to chemistry students. Sodium hydroxide is used in many industries: in the manufacture of pulp and paper, textiles, drinking water, soaps and deterge ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xanthoproteic Acid
Xanthoproteic acid is a noncrystallizable yellow substance derived from proteins upon treatment with nitric acid. Nitric acid reacts with proteins to form xanthoproteic acid. This reaction is known as the xanthoproteic reaction. This test is carried out by adding concentrated nitric acid to the substance being tested, and then heating the mixture. If proteins are present that contains amino acids with aromatic rings, the mixture turns yellow . Upon adding a strong base such as liquid ammonia, the color turns orange . These color changes are caused by nitrated aromatic rings in the protein . The xanthoproteic test is specific for aromatic compounds such as tyrosine, tryptophan and phenylalanine . Xanthoproteinic acids are also formed when the acid contacts the skin and are a certain giveaway of inadequate care when handling nitric acid . Minor stains are harmless and resolve in a few days . It is a mixture of nitro compounds of tyrosine, tryptophan, and phenylalanine such as 2-am ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Keratin
Keratin () is one of a family of structural fibrous proteins also known as ''scleroproteins''. Alpha-keratin (α-keratin) is a type of keratin found in vertebrates. It is the key structural material making up scales, hair, nails, feathers, horns, claws, hooves, and the outer layer of skin among vertebrates. Keratin also protects epithelial cells from damage or stress. Keratin is extremely insoluble in water and organic solvents. Keratin monomers assemble into bundles to form intermediate filaments, which are tough and form strong unmineralized epidermal appendages found in reptiles, birds, amphibians, and mammals. Excessive keratinization participate in fortification of certain tissues such as in horns of cattle and rhinos, and armadillos' osteoderm. The only other biological matter known to approximate the toughness of keratinized tissue is chitin. Keratin comes in two types, the primitive, softer forms found in all vertebrates and harder, derived forms found only amon ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nail (anatomy)
A nail is a claw-like plate found at the tip of the Finger, fingers and Toe, toes on most primates. Nails correspond to the claws found in other animals. Fingernails and toenails are made of a tough protective protein called alpha-keratin, which is a polymer. Alpha-keratin is found in the hooves, claws, and horns of vertebrates. Structure The nail consists of the nail plate, the nail matrix and the nail bed below it, and the grooves surrounding it. Parts of the nail The matrix, sometimes called the ''matrix unguis'', keratogenous membrane, nail matrix, or onychostroma, is the active Tissue (biology), tissue (or Germ layer, germinal Matrix (biology), matrix) that generates cells, which harden as they move outward from the nail root to the nail plate. It is the part of the nail bed that is beneath the nail and contains nerves, lymph and blood vessels. The matrix produces cells that become the nail plate. The width and thickness of the nail plate is determined by the size, length, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Aromatic Amino Acid
An aromatic amino acid is an amino acid that includes an aromatic ring. Among the 20 standard amino acids, the following are classically considered aromatic: phenylalanine, tryptophan and tyrosine. Although histidine contains an aromatic ring, its basic properties cause it to be predominantly classified as a polar amino acid. Chemical structure and properties Aromatic amino acids absorb ultraviolet light at a wavelength above 250 nm and produce fluorescence. This characteristic is used in quantitative analysis, notably in determining the concentrations of these amino acids in solution. This achieved through the utilization of a UV spectrophotomer and the Beer-Lambert Law equation. Most proteins will have an absorption maximum at 280 nm due to the presence of aromatic amino acids in their primary structure. However, because several aromatic amino acids exist, this method has low accuracy; in order to mitigate this issue, the desired protein must be pure, and its molar absorp ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Orange (colour)
Orange is the colour between yellow and red on the visible spectrum, spectrum of light, visible light. Human eyes perceive orange when observing light with a dominant wavelength between roughly 585 and 620 nanometres. In traditional colour theory, it is a secondary colour of pigments, produced by mixing yellow and red. In the RGB colour model, it is a tertiary colour. It is named after the orange (fruit), fruit of the same name. The orange colour of many fruits and vegetables, such as carrots, pumpkins, sweet potatoes, and Orange (fruit), oranges, comes from carotenes, a type of photosynthetic pigment. These pigments convert the light energy that the plants absorb from the Sun into chemical energy for the plants' growth. Similarly, the hues of autumn leaves are from the same pigment after chlorophyll is removed. In Europe and America, surveys show that orange is the colour most associated with amusement, the unconventional, extroversion, warmth, fire, energy, activity, danger ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Yellow
Yellow is the color between green and orange on the spectrum of light. It is evoked by light with a dominant wavelength of roughly 575585 nm. It is a primary color in subtractive color systems, used in painting or color printing. In the RGB color model, used to create colors on television and computer screens, yellow is a secondary color made by combining red and green at equal intensity. Carotenoids give the characteristic yellow color to autumn leaves, corn, canaries, daffodils, and lemons, as well as egg yolks, buttercups, and bananas. They absorb light energy and protect plants from photo damage in some cases. Sunlight has a slight yellowish hue when the Sun is near the horizon, due to atmospheric scattering of shorter wavelengths (green, blue, and violet). Because it was widely available, yellow ochre pigment was one of the first colors used in art; the Lascaux cave in France has a painting of a yellow horse 17,000 years old. Ochre and orpiment pigments were us ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nitric Acid
Nitric acid is the inorganic compound with the formula . It is a highly corrosive mineral acid. The compound is colorless, but older samples tend to be yellow cast due to decomposition into oxides of nitrogen. Most commercially available nitric acid has a concentration of 68% in water. When the solution contains more than 86% , it is referred to as ''fuming nitric acid''. Depending on the amount of nitrogen dioxide present, fuming nitric acid is further characterized as red fuming nitric acid at concentrations above 86%, or white fuming nitric acid at concentrations above 95%. Nitric acid is the primary reagent used for nitration – the addition of a nitro group, typically to an organic molecule. While some resulting nitro compounds are shock- and thermally-sensitive explosives, a few are stable enough to be used in munitions and demolition, while others are still more stable and used as pigments in inks and dyes. Nitric acid is also commonly used as a strong oxidizing agen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Protein
Proteins are large biomolecules and macromolecules that comprise one or more long chains of amino acid residues. Proteins perform a vast array of functions within organisms, including catalysing metabolic reactions, DNA replication, responding to stimuli, providing structure to cells and organisms, and transporting molecules from one location to another. Proteins differ from one another primarily in their sequence of amino acids, which is dictated by the nucleotide sequence of their genes, and which usually results in protein folding into a specific 3D structure that determines its activity. A linear chain of amino acid residues is called a polypeptide. A protein contains at least one long polypeptide. Short polypeptides, containing less than 20–30 residues, are rarely considered to be proteins and are commonly called peptides. The individual amino acid residues are bonded together by peptide bonds and adjacent amino acid residues. The sequence of amino acid residue ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Xanthoproteic Reaction
The xanthoproteic reaction is a method that can be used to detect a presence of protein soluble in a solution, using concentrated nitric acid. The test gives a positive result in amino acids carrying aromatic groups, especially in the presence of tyrosine. If the test is positive the proof is neutralized with an alkali, turning dark yellow. The yellow colour is due to xanthoproteic acid which is formed due to nitration of certain amino acids, most common examples being tyrosine and tryptophan. This chemical reaction is a qualitative test, determining the presence or absence of proteins. Procedure Add 1 ml of concentrated HNO3 to 1 ml of the test sample. Heat the mixture and cool it. Slowly add sodium hydroxide (40% w/v in water) solution until the mixture becomes alkaline and a colour change is noted. If the colour changes from yellow to orange, this indicates the presence of an aromatic amino acid. When human skin or nails are exposed to nitric acid, they turn yellow after ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
