Autoinducer 2
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Autoinducer 2
Autoinducer-2 (AI-2), a furanosyl borate diester or tetrahydroxy furan (species dependent), is a member of a family of signaling molecules used in quorum sensing. AI-2 is one of only a few known biomolecules incorporating boron. First identified in the marine bacterium ''Vibrio harveyi'', AI-2 is produced and recognized by many Gram-negative and Gram-positive bacteria. AI-2 arises by the reaction of 4,5-Dihydroxy-2,3-pentanedione, which is produced enzymatically with boric acid, and is recognized by the two-component sensor kinase LuxPQ in Vibrionaceae. AI-2 is actively transported by the Lsr ABC-type transporter into the cell in Enterobacteriaceae and few other bacterial taxa ('' Pasteurella'', ''Photorhabdus'', ''Haemophilus ''Haemophilus'' is a genus of Gram-negative, pleomorphic, coccobacilli bacteria belonging to the family Pasteurellaceae. While ''Haemophilus'' bacteria are typically small coccobacilli, they are categorized as pleomorphic bacteria because of ...' ...
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Borate Ester
In organic chemistry, borate esters are organoboron compounds which are conveniently prepared by the stoichiometric condensation reaction of boric acid with alcohols. There are two main classes of borate esters: orthoborates, and metaborates, . Metaborates contain 6-membered boroxine rings. :B(OH)3 + 3 ROH -> B(OR)3 + 3 H2O :3 B(OH)3 + 3 ROH -> B3O3(OR)3 + 6 H2O A dehydrating agent, such as concentrated sulfuric acid is typically added. Borate esters are volatile and can be purified by distillation. This procedure is used for analysis of trace amounts of borate and for analysis of boron in steel. Like all boron compounds, alkyl borates burn with a characteristic green flame. This property is used to determine the presence of boron in qualitative analysis. Borate esters form spontaneously when treated with diols such as sugars and the reaction with mannitol forms the basis of a titrimetric analytical method for boric acid. Metaborate esters show considerable Lewis acidity a ...
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Vibrionaceae
The Vibrionaceae are a family of Pseudomonadota given their own order, Vibrionales. Inhabitants of fresh or salt water, several species are pathogenic, including the type species ''Vibrio cholerae'', which is the agent responsible for cholera. Most bioluminescent bacteria belong to this family, and are typically found as symbionts of deep-sea animals. Vibrionaceae are Gram-negative organisms and facultative anaerobes, capable of fermentation. They contain oxidase and have one or more flagella, which are generally polar. Originally, these characteristics defined the family, which was divided into four genera. Two of these, ''Vibrio'' and ''Photobacterium'', correspond to the modern group, although several new genera have been defined. Genetic studies have shown the other two original members—''Aeromonas'' and '' Plesiomonas''—belong to separate families. The family Vibrionaceae currently comprises eight validly published genera: ''Aliivibrio'', ''Catenococcus'', ''Ent ...
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Cell Communication
In biology, cell signaling (cell signalling in British English) or cell communication is the ability of a Cell (biology), cell to receive, process, and transmit signals with its environment and with itself. Cell signaling is a fundamental property of all Cell (biology), cellular life in prokaryotes and eukaryotes. Signals that originate from outside a cell (or extracellular signals) can be physical agents like mechanical pressure, membrane potential , voltage, temperature, light, or chemical signals (e.g., small molecules, peptides, or gas). Cell signaling can occur over short or long distances, and as a result can be classified as autocrine, juxtacrine, intracrine, paracrine, or endocrine. Signaling molecules can be synthesized from various biosynthetic pathways and released through passive transport , passive or active transports, or even from cell damage. Receptor (biochemistry), Receptors play a key role in cell signaling as they are able to detect chemical signals or physic ...
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Cell Biology
Cell biology (also cellular biology or cytology) is a branch of biology that studies the structure, function, and behavior of cells. All living organisms are made of cells. A cell is the basic unit of life that is responsible for the living and functioning of organisms. Cell biology is the study of structural and functional units of cells. Cell biology encompasses both prokaryotic and eukaryotic cells and has many subtopics which may include the study of cell metabolism, cell communication, cell cycle, biochemistry, and cell composition. The study of cells is performed using several microscopy techniques, cell culture, and cell fractionation. These have allowed for and are currently being used for discoveries and research pertaining to how cells function, ultimately giving insight into understanding larger organisms. Knowing the components of cells and how cells work is fundamental to all biological sciences while also being essential for research in biomedical fields such as ...
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Signal Transduction
Signal transduction is the process by which a chemical or physical signal is transmitted through a cell as a series of molecular events, most commonly protein phosphorylation catalyzed by protein kinases, which ultimately results in a cellular response. Proteins responsible for detecting stimuli are generally termed receptors, although in some cases the term sensor is used. The changes elicited by ligand binding (or signal sensing) in a receptor give rise to a biochemical cascade, which is a chain of biochemical events known as a signaling pathway. When signaling pathways interact with one another they form networks, which allow cellular responses to be coordinated, often by combinatorial signaling events. At the molecular level, such responses include changes in the transcription or translation of genes, and post-translational and conformational changes in proteins, as well as changes in their location. These molecular events are the basic mechanisms controlling cell growth, ...
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S-adenosyl-L-methionine
''S''-Adenosyl methionine (SAM), also known under the commercial names of SAMe, SAM-e, or AdoMet, is a common cosubstrate involved in methyl group transfers, transsulfuration, and aminopropylation. Although these anabolic reactions occur throughout the body, most SAM is produced and consumed in the liver. More than 40 methyl transfers from SAM are known, to various substrates such as nucleic acids, proteins, lipids and secondary metabolites. It is made from adenosine triphosphate (ATP) and methionine by methionine adenosyltransferase. SAM was first discovered by Giulio Cantoni in 1952. In bacteria, SAM is bound by the SAM riboswitch, which regulates genes involved in methionine or cysteine biosynthesis. In eukaryotic cells, SAM serves as a regulator of a variety of processes including DNA, tRNA, and rRNA methylation; immune response; amino acid metabolism; transsulfuration; and more. In plants, SAM is crucial to the biosynthesis of ethylene, an important plant hormone and sign ...
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Journal Of Bacteriology
The ''Journal of Bacteriology'' is a biweekly peer-reviewed scientific journal established in 1916. It is published by the American Society for Microbiology and the editor in chief is George A. O'Toole (Dartmouth College). The journal is delayed open access: content is available for free at the journal's website and at PubMed Central after a six-month embargo. The journal is also hybrid open access allowing authors to pay an article processing fee for their articles to be available for free immediately. Abstracting and indexing The journal is abstracted and indexed in: According to the ''Journal Citation Reports'', the journal has a 2020 impact factor The impact factor (IF) or journal impact factor (JIF) of an academic journal is a scientometric index calculated by Clarivate that reflects the yearly mean number of citations of articles published in the last two years in a given journal, as i ... of 3.490. References External links *{{Official website, http://jb.asm.org/ ...
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Bacillus
''Bacillus'' (Latin "stick") is a genus of Gram-positive, rod-shaped bacteria, a member of the phylum ''Bacillota'', with 266 named species. The term is also used to describe the shape (rod) of other so-shaped bacteria; and the plural ''Bacilli'' is the name of the class of bacteria to which this genus belongs. ''Bacillus'' species can be either obligate aerobes which are dependent on oxygen, or facultative anaerobes which can survive in the absence of oxygen. Cultured ''Bacillus'' species test positive for the enzyme catalase if oxygen has been used or is present. ''Bacillus'' can reduce themselves to oval endospores and can remain in this dormant state for years. The endospore of one species from Morocco is reported to have survived being heated to 420 °C. Endospore formation is usually triggered by a lack of nutrients: the bacterium divides within its cell wall, and one side then engulfs the other. They are not true spores (i.e., not an offspring). Endospore formation ...
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Haemophilus
''Haemophilus'' is a genus of Gram-negative, pleomorphic, coccobacilli bacteria belonging to the family Pasteurellaceae. While ''Haemophilus'' bacteria are typically small coccobacilli, they are categorized as pleomorphic bacteria because of the wide range of shapes they occasionally assume. These organisms inhabit the mucous membranes of the upper respiratory tract, mouth, vagina, and intestinal tract. The genus includes commensal organisms along with some significant pathogenic species such as ''H. influenzae''—a cause of sepsis and bacterial meningitis in young children—and '' H. ducreyi'', the causative agent of chancroid. All members are either aerobic or facultatively anaerobic. This genus has been found to be part of the salivary microbiome. Metabolism Members of the genus ''Haemophilus'' will not grow on blood agar plates, as all species require at least one of these blood factors for growth: hemin (X-factor) and/or nicotinamide adenine dinucleotide (V-fa ...
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Photorhabdus
''Photorhabdus'' is a genus of bioluminescent, gram-negative bacilli which lives symbiotically within entomopathogenic nematodes, hence the name ''photo'' (which means light producing) and ''rhabdus'' (rod shape). ''Photorhabdus'' is known to be pathogenic to a wide range of insects and has been used as biopesticide in agriculture. Life cycle ''Photorhabdus'' species facilitate the reproduction of entomopathogenic nematodes by infecting and killing susceptible insect larvae. Entomopathogenic nematodes are normally found in soil. Nematodes infect larval hosts by piercing the larval cuticle. When the nematode enters an insect larvae, ''Photorhabdus'' species are released by the nematodes and will produce a range of toxins, killing the host within 48 hours. ''Photorhabdus'' species feed on the cadaver of the insect and the process converts the cadaver into a nutrient source for the nematode. Mature nematodes leave the depleted body of the insect and search for new hosts to infect. ...
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Pasteurella
__NOTOC__ ''Pasteurella'' is a genus of Gram-negative, facultatively anaerobic bacteria. ''Pasteurella'' species are nonmotile and pleomorphic, and often exhibit bipolar staining ("safety pin" appearance). Most species are catalase- and oxidase-positive. The genus is named after the French chemist and microbiologist, Louis Pasteur, who first identified the bacteria now known as ''Pasteurella multocida'' as the agent of chicken cholera. Pathogenesis Many ''Pasteurella'' species are zoonotic pathogens, and humans can acquire an infection from domestic animal bites. In cattle, sheep, and birds, ''Pasteurella'' species can cause a life-threatening pneumonia; in cats and dogs, however, ''Pasteurella'' is not a cause of disease, and constitutes part of the normal flora of the nose and mouth. ''Pasteurella haemolytica'' is a species that infects mainly cattle and horses: '' P. multocida'' is the most frequent causative agent in human ''Pasteurella'' infection. Common symptoms of pasteu ...
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