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Tethered Particle Motion
Tethered particle motion (TPM) is a biophysical method that is used for studying various polymers such as DNA and their interaction with other entities such as proteins. The method allows observers to measure various physical properties on the substances, as well as to measure the properties of biochemical interactions with other substances such as proteins and enzymes. TPM is a single molecule experiment method. History TPM was first introduced by Schafer, Gelles, Sheetz and Landick in 1991. In their research, they attached RNA polymerase to the surface, and gold beads were attached to one end of the DNA molecules. In the beginning, the RNA polymerase "captures" the DNA near the gold bead. During the transcription, the DNA "slides" on the RNA polymerase so the distance between the RNA polymerase and the gold bead (the tether length)is increased. Using an optical microscope the area that the bead moves in was detected. The transcription rate was extracted from data. Since then, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Polymers
A polymer (; Greek '' poly-'', "many" + ''-mer'', "part") is a substance or material consisting of very large molecules called macromolecules, composed of many repeating subunits. Due to their broad spectrum of properties, both synthetic and natural polymers play essential and ubiquitous roles in everyday life. Polymers range from familiar synthetic plastics such as polystyrene to natural biopolymers such as DNA and proteins that are fundamental to biological structure and function. Polymers, both natural and synthetic, are created via polymerization of many small molecules, known as monomers. Their consequently large molecular mass, relative to small molecule compounds, produces unique physical properties including toughness, high elasticity, viscoelasticity, and a tendency to form amorphous and semicrystalline structures rather than crystals. The term "polymer" derives from the Greek word πολύς (''polus'', meaning "many, much") and μέρος (''meros'', meani ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Dichroic Filter
A dichroic filter, thin-film filter, or interference filter is a color filter used to selectively pass light of a small range of colors while reflecting other colors. By comparison, dichroic mirrors and dichroic reflectors tend to be characterized by the colors of light that they reflect, rather than the colors they pass. Dichroic filters can filter light from a white light source to produce light that is perceived by humans to be highly saturated in color. Such filters are popular in architectural and theatrical applications. Dichroic reflectors known as cold mirrors are commonly used behind a light source to reflect visible light forward while allowing the invisible infrared light to pass out of the rear of the fixture. Such an arrangement allows intense illumination with less heating of the illuminated object. Many quartz-halogen lamps have an integrated dichroic reflector for this purpose, being originally designed for use in slide projectors to avoid melting the slides, ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Random Walk
In mathematics, a random walk is a random process that describes a path that consists of a succession of random steps on some mathematical space. An elementary example of a random walk is the random walk on the integer number line \mathbb Z which starts at 0, and at each step moves +1 or −1 with equal probability. Other examples include the path traced by a molecule as it travels in a liquid or a gas (see Brownian motion), the search path of a foraging animal, or the price of a fluctuating stock and the financial status of a gambler. Random walks have applications to engineering and many scientific fields including ecology, psychology, computer science, physics, chemistry, biology, economics, and sociology. The term ''random walk'' was first introduced by Karl Pearson in 1905. Lattice random walk A popular random walk model is that of a random walk on a regular lattice, where at each step the location jumps to another site according to some probability distribution. In a ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
High-pass Filter
A high-pass filter (HPF) is an electronic filter that passes signals with a frequency higher than a certain cutoff frequency and attenuates signals with frequencies lower than the cutoff frequency. The amount of attenuation for each frequency depends on the filter design. A high-pass filter is usually modeled as a linear time-invariant system. It is sometimes called a low-cut filter or bass-cut filter in the context of audio engineering. High-pass filters have many uses, such as blocking DC from circuitry sensitive to non-zero average voltages or radio frequency devices. They can also be used in conjunction with a low-pass filter to produce a bandpass filter. In the optical domain filters are often characterised by wavelength rather than frequency. High-pass and low-pass have the opposite meanings, with a "high-pass" filter (more commonly "long-pass") passing only ''longer'' wavelengths (lower frequencies), and vice versa for "low-pass" (more commonly "short-pass"). Descripti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
TPM Drift Correction
TPM may refer to: Organisations * The Pentecostal Mission, a Pentecostal religious denomination in Tamil Nadu, India * Australian Securities Exchange symbol for TPG Telecom Publishing * ''The Philosophers' Magazine'', an independent quarterly magazine * ''Talking Points Memo'', a political blog run by Joshua Marshall Science and technology * Tethered particle motion, a biophysical single-molecule experiment method * Technical program manager, a product manager with a background or a focus on engineering * Transcripts per million, a measure of gene expression in RNA-Seq. Computing * Trusted Platform Module, a specification for a secure cryptoprocessor included with some computers * Tivoli Provisioning Manager, a software product by IBM * Trade promotion management, software that supports the management of trade promotion * Technical protection measures, another name for digital rights management Other uses * Technical performance measure, a term used by the US military for key ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Center Of Mass
In physics, the center of mass of a distribution of mass in space (sometimes referred to as the balance point) is the unique point where the weighted relative position of the distributed mass sums to zero. This is the point to which a force may be applied to cause a linear acceleration without an angular acceleration. Calculations in mechanics are often simplified when formulated with respect to the center of mass. It is a hypothetical point where the entire mass of an object may be assumed to be concentrated to visualise its motion. In other words, the center of mass is the particle equivalent of a given object for application of Newton's laws of motion. In the case of a single rigid body, the center of mass is fixed in relation to the body, and if the body has uniform density, it will be located at the centroid. The center of mass may be located outside the physical body, as is sometimes the case for hollow or open-shaped objects, such as a horseshoe. In the case of a dist ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Gaussian Function
In mathematics, a Gaussian function, often simply referred to as a Gaussian, is a function of the base form f(x) = \exp (-x^2) and with parametric extension f(x) = a \exp\left( -\frac \right) for arbitrary real constants , and non-zero . It is named after the mathematician Carl Friedrich Gauss. The graph of a Gaussian is a characteristic symmetric " bell curve" shape. The parameter is the height of the curve's peak, is the position of the center of the peak, and (the standard deviation, sometimes called the Gaussian RMS width) controls the width of the "bell". Gaussian functions are often used to represent the probability density function of a normally distributed random variable with expected value and variance . In this case, the Gaussian is of the form g(x) = \frac \exp\left( -\frac \frac \right). Gaussian functions are widely used in statistics to describe the normal distributions, in signal processing to define Gaussian filters, in image processing where two-dimensio ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Airy Disk
In optics, the Airy disk (or Airy disc) and Airy pattern are descriptions of the best- focused spot of light that a perfect lens with a circular aperture can make, limited by the diffraction of light. The Airy disk is of importance in physics, optics, and astronomy. The diffraction pattern resulting from a uniformly illuminated, circular aperture has a bright central region, known as the Airy disk, which together with the series of concentric rings around is called the Airy pattern. Both are named after George Biddell Airy. The disk and rings phenomenon had been known prior to Airy; John Herschel described the appearance of a bright star seen through a telescope under high magnification for an 1828 article on light for the ''Encyclopedia Metropolitana'': Airy wrote the first full theoretical treatment explaining the phenomenon (his 1835 "On the Diffraction of an Object-glass with Circular Aperture"). Mathematically, the diffraction pattern is characterized by the wavelen ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Focal Plane
In Gaussian optics, the cardinal points consist of three pairs of points located on the optical axis of a rotationally symmetric, focal, optical system. These are the '' focal points'', the principal points, and the nodal points. For ''ideal'' systems, the basic imaging properties such as image size, location, and orientation are completely determined by the locations of the cardinal points; in fact only four points are necessary: the focal points and either the principal or nodal points. The only ideal system that has been achieved in practice is the plane mirror, however the cardinal points are widely used to ''approximate'' the behavior of real optical systems. Cardinal points provide a way to analytically simplify a system with many components, allowing the imaging characteristics of the system to be approximately determined with simple calculations. Explanation The cardinal points lie on the optical axis of the optical system. Each point is defined by the effect the opti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Pixel
In digital imaging, a pixel (abbreviated px), pel, or picture element is the smallest addressable element in a raster image, or the smallest point in an all points addressable display device. In most digital display devices, pixels are the smallest element that can be manipulated through software. Each pixel is a sample of an original image; more samples typically provide more accurate representations of the original. The intensity of each pixel is variable. In color imaging systems, a color is typically represented by three or four component intensities such as red, green, and blue, or cyan, magenta, yellow, and black. In some contexts (such as descriptions of camera sensors), ''pixel'' refers to a single scalar element of a multi-component representation (called a ''photosite'' in the camera sensor context, although ''sensel'' is sometimes used), while in yet other contexts (like MRI) it may refer to a set of component intensities for a spatial position. Etymology The w ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
TPM Experiment Video
TPM may refer to: Organisations * The Pentecostal Mission, a Pentecostal religious denomination in Tamil Nadu, India * Australian Securities Exchange symbol for TPG Telecom Publishing * ''The Philosophers' Magazine'', an independent quarterly magazine * ''Talking Points Memo'', a political blog run by Joshua Marshall Science and technology * Tethered particle motion, a biophysical single-molecule experiment method * Technical program manager, a product manager with a background or a focus on engineering * Transcripts per million, a measure of gene expression in RNA-Seq. Computing * Trusted Platform Module, a specification for a secure cryptoprocessor included with some computers * Tivoli Provisioning Manager, a software product by IBM * Trade promotion management, software that supports the management of trade promotion * Technical protection measures, another name for digital rights management Other uses * Technical performance measure, a term used by the US military for key ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
