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TSI Agar
250px, TSI agar slant results: (from left) preinoculated (as control), ''P. aeruginosa'', ''E. coli'', '' Salmonella Typhimurium'', ''Shigella flexneri'' ">Shigella_flexneri.html" ;"title="Salmonella Typhimurium'', ''Shigella flexneri">Salmonella Typhimurium'', ''Shigella flexneri'' The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. It is often used to Differential media, differentiate enteric bacteria including ''Salmonella'' and ''Shigella''. Composition The TSI slant is a test tube that contains agar, a pH-sensitive dye (phenol red), 1% lactose, 1% sucrose, 0.1% glucose, and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle. The slanted shape of this medium provides an array of surfaces that are either expo ...
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Ferrous Sulfate
Iron(II) sulfate (British English: iron(II) sulphate) or ferrous sulfate denotes a range of salts with the formula Fe SO4·''x''H2O. These compounds exist most commonly as the hepta hydrate (''x'' = 7) but several values for x are known. The hydrated form is used medically to treat iron deficiency, and also for industrial applications. Known since ancient times as copperas and as green vitriol (vitriol is an archaic name for sulfate), the blue-green heptahydrate ( hydrate with 7 molecules of water) is the most common form of this material. All the iron(II) sulfates dissolve in water to give the same aquo complex e(H2O)6sup>2+, which has octahedral molecular geometry and is paramagnetic. The name copperas dates from times when the copper(II) sulfate was known as blue copperas, and perhaps in analogy, iron(II) and zinc sulfate were known respectively as green and white copperas. It is on the World Health Organization's List of Essential Medicines. In 2020, it was the 1 ...
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Cystine Tryptic Agar
Cystine tryptic agar (CTA), also known as cystine trypticase agar, is a growth medium used for the identification of microorganisms. It can be used to determine if organisms can ferment various carbohydrates, including maltose, lactose, and sucrose. This approach can be used to type organisms because although strains quickly gain antibiotic resistance, they rarely gain the ability to metabolize new nutrients (though exceptions are known.) For example, the following fermentation patterns have been observed: Typical composition Cystine tryptic agar typically contains ( w/v):Becton, Dickinson and Company CTA Medium 2005. *2.0 % casein *0.05 % L-cystine *0.5 % sodium chloride *0.05% sodium sulfite *0.25% agar *0.00017% phenol red *0.5% one of the sugars mentioned above See also * TSI slant 250px, TSI agar slant results: (from left) preinoculated (as control), ''P. aeruginosa'', ''E. coli'', '' Salmonella Typhimurium'', ''Shigella flexneri'' ">Shigella_flexneri.html" ;"title= ...
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Hydrogen Production
Hydrogen production is the family of industrial methods for generating hydrogen gas. As of 2020, the majority of hydrogen (∼95%) is produced from fossil fuels by steam reforming of natural gas and other light hydrocarbons, partial oxidation of heavier hydrocarbons, and coal gasification. Other methods of hydrogen production include biomass gasification, zero-CO2-emission methane pyrolysis, and electrolysis of water. The latter processes, methane pyrolysis as well as water electrolysis can be done directly with any source of electricity, such as solar power. The production of hydrogen plays a key role in any industrialized society, since hydrogen is required for many essential chemical processes. In 2020, roughly 87 million tons of hydrogen was produced worldwide for various uses, such as oil refining, and in the production of ammonia (NH3) (through the Haber process) and methanol (CH3OH) (through reduction of carbon monoxide O, and also as a fuel in transportation. The glo ...
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TSI Agar
250px, TSI agar slant results: (from left) preinoculated (as control), ''P. aeruginosa'', ''E. coli'', '' Salmonella Typhimurium'', ''Shigella flexneri'' ">Shigella_flexneri.html" ;"title="Salmonella Typhimurium'', ''Shigella flexneri">Salmonella Typhimurium'', ''Shigella flexneri'' The Triple Sugar Iron (TSI) test is a microbiological test roughly named for its ability to test a microorganism's ability to ferment sugars and to produce hydrogen sulfide. It is often used to Differential media, differentiate enteric bacteria including ''Salmonella'' and ''Shigella''. Composition The TSI slant is a test tube that contains agar, a pH-sensitive dye (phenol red), 1% lactose, 1% sucrose, 0.1% glucose, and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle. The slanted shape of this medium provides an array of surfaces that are either expo ...
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Salmonella Enterica
''Salmonella enterica'' (formerly ''Salmonella choleraesuis'') is a rod-headed, flagellate, facultative anaerobic, Gram-negative bacterium and a species of the genus ''Salmonella''. A number of its serovars are serious human pathogens. Epidemiology Most cases of salmonellosis are caused by food infected with ''S. enterica'', which often infects cattle and poultry, though other animals such as domestic cats and hamsters have also been shown to be sources of infection in humans. Investigations of vacuum cleaner bags have shown that households can act as a reservoir of the bacterium; this is more likely if the household has contact with an infection source (i.e., members working with cattle or in a veterinary clinic). Raw chicken eggs and goose eggs can harbor ''S. enterica'', initially in the egg whites, although most eggs are not infected. As the egg ages at room temperature, the yolk membrane begins to break down and ''S. enterica'' can spread into the yolk. Refrigeration a ...
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Edwardsiella (bacterium)
''Edwardsiella'' is a genus of gram-negative, fermentative bacteria of the family Hafniaceae. It was first discovered in snakes in 1962. Description A genus of gram-negative, facultatively anaerobic, rod-shaped bacteria of the family Hafniaceae, they are occasionally opportunistic pathogens of humans. Species Notable species include: * ''Edwardsiella hoshinae'', a motile species that, isolated from animals and humans, does not produce indole. * ''Edwardsiella ictaluri'', a nonmotile species that does not produce indole, and occurs as a pathogen of catfish. * ''Edwardsiella tarda'', also known as ''Edwardsiella anguillimortifera'', a species which produces indole, is biochemically similar to ''Escherichia coli ''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus '' Esc ...''. It is usual ...
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Citrobacter
''Citrobacter'' is a genus of Gram-negative coliform bacteria in the family Enterobacteriaceae. The species ''C. amalonaticus'', ''C. koseri'', and ''C. freundii'' can use citrate as a sole carbon source. ''Citrobacter'' species are differentiated by their ability to convert tryptophan to indole (''C. koseri'' is the only citrobacter to be commonly indole-positive), ferment lactose (''C. koseri'' is a lactose fermentor), and use malonate. ''Citrobacter'' shows the ability to accumulate uranium by building phosphate complexes. Clinical significance These bacteria can be found almost everywhere in soil, water, wastewater, etc. They can also be found in the human intestine. They are rarely the source of illnesses, except for infections of the GI Tract, urinary tract and infant meningitis and sepsis. ''Citrobacter freundii'' strains have inducible ''ampC'' genes encoding resistance to ampicillin and first-generation cephalosporins. In addition, isolates of ''Citrobacter'' may be r ...
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Proteus (bacterium)
''Proteus'' is a genus of Gram-negative bacteria. ''Proteus'' bacilli are widely distributed in nature as saprophytes, being found in decomposing animal matter, sewage, manure soil, the mammalian intestine, and human and animal feces. They are opportunistic pathogens, commonly responsible for urinary and septic infections, often nosocomial. The term Proteus signifies changeability of form, as personified in the Homeric poems in Proteus, "the old man of the sea", who tends the sealflocks of Poseidon and has the gift of endless transformation. The first use of the term “Proteus” in bacteriological nomenclature was made by Hauser (1885), who described under this term three types of organisms which he isolated from putrefied meat. Clinical significance Three species—'' P. vulgaris'', '' P. mirabilis'', and '' P. penneri''—are opportunistic human pathogens. ''Proteus'' includes pathogens responsible for many human urinary tract infections. ''P. mirabilis'' causes w ...
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Ferrous Sulfide
Iron(II) sulfide or ferrous sulfide (Br.E. sulphide) is one of a family chemical compounds and minerals with the approximate formula . Iron sulfides are often iron-deficient non-stoichiometric. All are black, water-insoluble solids. Preparation and structure FeS can be obtained by the heating of iron and sulfur: :Fe + S → FeS FeS adopts the nickel arsenide structure, featuring octahedral Fe centers and trigonal prismatic sulfide sites. Reactions Iron sulfide reacts with hydrochloric acid, releasing hydrogen sulfide: :FeS + 2 HCl → FeCl2 + H2S :FeS + H2SO4 → FeSO4 + H2S In moist air, iron sulfides oxidize to hydrated ferrous sulfate. Biology and biogeochemistry Iron sulfides occur widely in nature in the form of iron–sulfur proteins. As organic matter decays under low-oxygen (or hypoxic) conditions such as in swamps or dead zones of lakes and oceans, sulfate-reducing bacteria reduce various sulfates present in the water, producing hydrogen sulfide. S ...
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Ferrous Ammonium Sulfate
Ammonium iron(II) sulfate, or Mohr's salt, is the inorganic compound with the formula (NH4)2Fe(SO4)2(H2O)6. Containing two different cations, Fe2+ and NH4+, it is classified as a double salt of ferrous sulfate and ammonium sulfate. It is a common laboratory reagent because it is readily crystallized, and crystals resist oxidation by air. Like the other ferrous sulfate salts, ferrous ammonium sulfate dissolves in water to give the aquo complex e(H2O)6sup>2+, which has octahedral molecular geometry. Its mineral form is mohrite. Structure This compound is a member of a group of double sulfates called Schönites or Tutton's salts. Tutton's salts form monoclinic crystals and have formula M2N(SO4)2.6H2O (M = various monocations). With regards to the bonding, crystals consist of octahedra e(H2O)6sup>2+ centers, which are hydrogen bonded to sulfate and ammonium. Mohr's salt is named after the German chemist Karl Friedrich Mohr, who made many important advances in the methodology o ...
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Sodium Thiosulfate
Sodium thiosulfate (sodium thiosulphate) is an inorganic compound with the formula . Typically it is available as the white or colorless pentahydrate, . The solid is an efflorescent (loses water readily) crystalline substance that dissolves well in water. Sodium thiosulfate is used in gold mining, water treatment, analytical chemistry, the development of silver-based photographic film and prints, and medicine. The medical uses of sodium thiosulfate include treatment of cyanide poisoning and pityriasis. It is on the World Health Organization's List of Essential Medicines. Uses Sodium thiosulfate is used predominantly in industry. For example, it is used to convert dyes to their soluble colorless forms, which are called leuco. It is also used to bleach "wool, cotton, silk, ...soaps, glues, clay, sand, bauxite, and... edible oils, edible fats, and gelatin." Medical uses Sodium thiosulfate is used in the treatment of cyanide poisoning. Other uses include topical treatment of ...
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