Syngas Fermentation
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Syngas Fermentation
Syngas fermentation, also known as synthesis gas fermentation, is a microbial process. In this process, a mixture of hydrogen, carbon monoxide, and carbon dioxide, known as syngas, is used as carbon and energy sources, and then converted into fuel and chemicals by microorganisms. The main products of syngas fermentation (biochemistry), fermentation include ethanol, butanol, acetic acid, butyric acid, and methane.Worden, R.M., Bredwell, M.D., and Grethlein, A.J. (1997). Engineering issues in synthesis gas fermentations, Fuels and Chemicals from Biomass. Washington, DC: American Chemical Society, 321-335 Certain industrial processes, such as petroleum refining, steel milling, and methods for producing carbon black, Petroleum coke, coke, ammonia, and methanol, discharge enormous amounts of waste gases containing mainly CO and into the atmosphere either directly or through combustion. Biocatalysts can be exploited to convert these waste gases to chemicals and fuels as, for example, eth ...
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Hydrogen
Hydrogen is the chemical element with the symbol H and atomic number 1. Hydrogen is the lightest element. At standard conditions hydrogen is a gas of diatomic molecules having the formula . It is colorless, odorless, tasteless, non-toxic, and highly combustible. Hydrogen is the most abundant chemical substance in the universe, constituting roughly 75% of all normal matter.However, most of the universe's mass is not in the form of baryons or chemical elements. See dark matter and dark energy. Stars such as the Sun are mainly composed of hydrogen in the plasma state. Most of the hydrogen on Earth exists in molecular forms such as water and organic compounds. For the most common isotope of hydrogen (symbol 1H) each atom has one proton, one electron, and no neutrons. In the early universe, the formation of protons, the nuclei of hydrogen, occurred during the first second after the Big Bang. The emergence of neutral hydrogen atoms throughout the universe occurred about 370,000 ...
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Ammonia
Ammonia is an inorganic compound of nitrogen and hydrogen with the formula . A stable binary hydride, and the simplest pnictogen hydride, ammonia is a colourless gas with a distinct pungent smell. Biologically, it is a common nitrogenous waste, particularly among aquatic organisms, and it contributes significantly to the nutritional needs of terrestrial organisms by serving as a precursor to 45% of the world's food and fertilizers. Around 70% of ammonia is used to make fertilisers in various forms and composition, such as urea and Diammonium phosphate. Ammonia in pure form is also applied directly into the soil. Ammonia, either directly or indirectly, is also a building block for the synthesis of many pharmaceutical products and is used in many commercial cleaning products. It is mainly collected by downward displacement of both air and water. Although common in nature—both terrestrially and in the outer planets of the Solar System—and in wide use, ammonia is both caust ...
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Chemical Specificity
Chemical specificity is the ability of binding site of a macromolecule (such as a protein) to bind specific ligands. The fewer ligands a protein can bind, the greater its specificity. Specificity describes the strength of binding between a given protein and ligand. This relationship can be described by a dissociation constant, which characterizes the balance between bound and unbound states for the protein-ligand system. In the context of a single enzyme and a pair of binding molecules, the two ligands can be compared as stronger or weaker ligands (for the enzyme) on the basis of their dissociation constants. (A lower value corresponds to a stronger binding.) Specificity for a set of ligands is unrelated to the ability of an enzyme to catalyze a given reaction, with the ligand as a substrate. If a given enzyme has a high chemical specificity, this means that the set of ligands to which it binds is limited, such that neither binding events nor catalysis can occur at an appreciabl ...
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Chemical Reaction
A chemical reaction is a process that leads to the IUPAC nomenclature for organic transformations, chemical transformation of one set of chemical substances to another. Classically, chemical reactions encompass changes that only involve the positions of electrons in the forming and breaking of chemical bonds between atoms, with no change to the Atomic nucleus, nuclei (no change to the elements present), and can often be described by a chemical equation. Nuclear chemistry is a sub-discipline of chemistry that involves the chemical reactions of unstable and radioactive Chemical element, elements where both electronic and nuclear changes can occur. The substance (or substances) initially involved in a chemical reaction are called reagent, reactants or reagents. Chemical reactions are usually characterized by a chemical change, and they yield one or more Product (chemistry), products, which usually have properties different from the reactants. Reactions often consist of a sequence o ...
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Pressure
Pressure (symbol: ''p'' or ''P'') is the force applied perpendicular to the surface of an object per unit area over which that force is distributed. Gauge pressure (also spelled ''gage'' pressure)The preferred spelling varies by country and even by industry. Further, both spellings are often used ''within'' a particular industry or country. Industries in British English-speaking countries typically use the "gauge" spelling. is the pressure relative to the ambient pressure. Various units are used to express pressure. Some of these derive from a unit of force divided by a unit of area; the SI unit of pressure, the pascal (Pa), for example, is one newton per square metre (N/m2); similarly, the pound-force per square inch (psi) is the traditional unit of pressure in the imperial and U.S. customary systems. Pressure may also be expressed in terms of standard atmospheric pressure; the atmosphere (atm) is equal to this pressure, and the torr is defined as of this. Manometric u ...
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Temperature
Temperature is a physical quantity that expresses quantitatively the perceptions of hotness and coldness. Temperature is measured with a thermometer. Thermometers are calibrated in various temperature scales that historically have relied on various reference points and thermometric substances for definition. The most common scales are the Celsius scale with the unit symbol °C (formerly called ''centigrade''), the Fahrenheit scale (°F), and the Kelvin scale (K), the latter being used predominantly for scientific purposes. The kelvin is one of the seven base units in the International System of Units (SI). Absolute zero, i.e., zero kelvin or −273.15 °C, is the lowest point in the thermodynamic temperature scale. Experimentally, it can be approached very closely but not actually reached, as recognized in the third law of thermodynamics. It would be impossible to extract energy as heat from a body at that temperature. Temperature is important in all fields of natur ...
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Chemical Process
In a scientific sense, a chemical process is a method or means of somehow changing one or more chemicals or chemical compounds. Such a chemical process can occur by itself or be caused by an outside force, and involves a chemical reaction of some sort. In an "engineering" sense, a chemical process is a method intended to be used in manufacturing or on an industrial scale (see Industrial process) to change the composition of chemical(s) or material(s), usually using technology similar or related to that used in chemical plants or the chemical industry. Neither of these definitions are exact in the sense that one can always tell definitively what is a chemical process and what is not; they are practical definitions. There is also significant overlap in these two definition variations. Because of the inexactness of the definition, chemists and other scientists use the term "chemical process" only in a general sense or in the engineering sense. However, in the "process (engineer ...
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Wood–Ljungdahl Pathway
The Wood–Ljungdahl pathway is a set of biochemical reactions used by some bacteria. It is also known as the reductive acetyl-coenzyme A (Acetyl-CoA) pathway. This pathway enables these organisms to use hydrogen as an electron donor, and carbon dioxide as an electron acceptor and as a building block for biosynthesis. In this pathway carbon dioxide is reduced to carbon monoxide and formic acid or directly into a formyl group, the formyl group is reduced to a methyl group and then combined with the carbon monoxide and Coenzyme A to produce acetyl-CoA. Two specific enzymes participate on the carbon monoxide side of the pathway: CO Dehydrogenase and acetyl-CoA synthase. The former catalyzes the reduction of the CO2 and the latter combines the resulting CO with a methyl group to give acetyl-CoA. Some anaerobic bacteria use the Wood–Ljungdahl pathway in reverse to break down acetate. For example, Sulfate reducing bacteria oxidize acetate completely to CO2 and H2 coupled with ...
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Peptostreptococcus
''Peptostreptococcus'' is a genus of anaerobic, Gram-positive, non-spore forming bacteria. The cells are small, spherical A sphere () is a geometrical object that is a three-dimensional analogue to a two-dimensional circle. A sphere is the set of points that are all at the same distance from a given point in three-dimensional space.. That given point is the ce ..., and can occur in short chains, in pairs or individually. They typically move using cilia. ''Peptostreptococcus'' are slow-growing bacteria with increasing resistance to antimicrobial drugs. ''Peptostreptococcus'' is a normal List of microbiota species of the lower reproductive tract of women, inhabitant of the healthy lower reproductive tract of women. Pathogenesis ''Peptostreptococcus'' species are commensal organisms in humans, living predominantly in the mouth, skin, gastrointestinal tract, gastrointestinal, vagina and urinary tracts, and are members of the gut flora, gut microbiota. Under immunosuppresse ...
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Clostridium Carboxidivorans
''Clostridium carboxidivorans'' is a Gram-positive anaerobic, spore-forming and motile bacterium from the genus ''Clostridium'' which has been isolated from an agricultural lagoon in Oklahoma in the United States. ''Clostridium carboxidivorans'' is capable of fermenting carbon monoxide Carbon monoxide (chemical formula CO) is a colorless, poisonous, odorless, tasteless, flammable gas that is slightly less dense than air. Carbon monoxide consists of one carbon atom and one oxygen atom connected by a triple bond. It is the simple ..., hydrogen, and carbon dioxide, and produce ethanol, butanol and hexanol as end-products. C. carboxidivorans does this using the wood-ljungdahl pathway. References Bacteria described in 2005 carboxidivorans {{Firmicutes-stub ...
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Eubacterium
''Eubacterium'' is a genus of Gram-positive bacteria in the family Eubacteriaceae. These bacteria are characterised by a rigid cell wall. They may either be motile or nonmotile. If motile, they have a flagellum A flagellum (; ) is a hairlike appendage that protrudes from certain plant and animal sperm cells, and from a wide range of microorganisms to provide motility. Many protists with flagella are termed as flagellates. A microorganism may have f .... A typical flagellum consists of a basal body, filament, and hook. The long filament is the organ which helps eubacteria move. Gram-positive bacteria have a thick proteoglycan layer and take up violet Gram stain (whereas Gram-negative bacteria have a thinner proteoglycan layer which is surrounded by a layer of immune response-inducing lipopolysaccharide, and do not take up Gram stain). References Eubacteriaceae Bacterial vaginosis Bacteria genera {{Clostridia-stub ...
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Clostridium Autoethanogenum
''Clostridium autoethanogenum'' is an anaerobic bacterium that produces ethanol from carbon monoxide, in so-called syngas fermentation, being one of the few known microorganisms to do so. It is gram-positive, spore-forming, rod-like, motile Motility is the ability of an organism to move independently, using metabolic energy. Definitions Motility, the ability of an organism to move independently, using metabolic energy, can be contrasted with sessility, the state of organisms th ..., and was first isolated from rabbit feces. Its type strain is strain JA1-1. Its genome has been sequenced and the genes required for utilising carbon monoxide as a sole carbon and energy source have been determined. Uses This species is the key to the technology developed by Lanzatech to create ethanol from waste carbon monoxide gas of factory emissions. References Further reading * * * External links * LPSN Gram-positive bacteria autoethanogenum {{Clostridia-stub ...
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