Sphingomonas Elodea
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Sphingomonas Elodea
''Sphingomonas elodea'' is a species of bacteria in the genus ''Sphingomonas''. This species is important to humans due to the fact that it produces gellan gum, a suitable agar substitute as a gelling agent in various clinical bacteriological media and especially important for the culture growth of thermophilic microorganisms in solid media. When the gellan gum-producing bacterium was first isolated from a natural lily pond it was classified as ''Pseudomonas elodea'' based on the taxonomic classification of that time. However, the gellan gum-producing bacterium was subsequently re-classified as '' Sphingomonas elodea'' based on the current taxonomic classification. ''Sphingomonas elodea'' metabolizes maltodextrin (oligosaccharides of glucose) externally into glucose by the putative exo-acting glucosidase. ''Sphingomonas elodea'' utilizes the Entner-Doudoroff pathway for glucose metabolism. References elodea ''Elodea'' is a genus of 6 species of aquatic plants often c ...
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Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one Cell (biology), biological cell. They constitute a large domain (biology), domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, Hot spring, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the nitrogen fixation, fixation of nitrogen from the Earth's atmosphere, atmosphere. The nutrient cycle includes the decomposition of cadaver, dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulp ...
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Pseudomonadota
Pseudomonadota (synonym Proteobacteria) is a major phylum of Gram-negative bacteria. The renaming of phyla in 2021 remains controversial among microbiologists, many of whom continue to use the earlier names of long standing in the literature. The phylum Proteobacteria includes a wide variety of pathogenic genera, such as '' Escherichia'', '' Salmonella'', '' Vibrio'', '' Yersinia'', '' Legionella'', and many others.Slonczewski JL, Foster JW, Foster E. Microbiology: An Evolving Science 5th Ed. WW Norton & Company; 2020. Others are free-living (non parasitic) and include many of the bacteria responsible for nitrogen fixation. Carl Woese established this grouping in 1987, calling it informally the "purple bacteria and their relatives". Because of the great diversity of forms found in this group, it was later informally named Proteobacteria, after Proteus, a Greek god of the sea capable of assuming many different shapes (not after the Proteobacteria genus ''Proteus''). In 2021 the I ...
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Alphaproteobacteria
Alphaproteobacteria is a class of bacteria in the phylum Pseudomonadota (formerly Proteobacteria). The Magnetococcales and Mariprofundales are considered basal or sister to the Alphaproteobacteria. The Alphaproteobacteria are highly diverse and possess few commonalities, but nevertheless share a common ancestor. Like all ''Proteobacteria'', its members are gram-negative and some of its intracellular parasitic members lack peptidoglycan and are consequently gram variable. Characteristics The Alphaproteobacteria are a diverse taxon and comprises several phototrophic genera, several genera metabolising C1-compounds (''e.g.'', ''Methylobacterium'' spp.), symbionts of plants (''e.g.'', ''Rhizobium'' spp.), endosymbionts of arthropods (''Wolbachia'') and intracellular pathogens (''e.g. Rickettsia''). Moreover, the class is sister to the protomitochondrion, the bacterium that was engulfed by the eukaryotic ancestor and gave rise to the mitochondria, which are organelles in eukaryotic ...
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Sphingomonadales
The ''Sphingomonadales'' are an order of the Alphaproteobacteria. Phylogeny The currently accepted taxonomy is based on the List of Prokaryotic names with Standing in Nomenclature List of Prokaryotic names with Standing in Nomenclature (LPSN) is an online database that maintains information on the nomenclature, naming and Taxonomy (biology), taxonomy of prokaryotes, following the taxonomy requirements and rulings of the In ... and the phylogeny is based on whole-genome sequences. References Sphingomonadales Bacteria orders Alphaproteobacteria {{Alphaproteobacteria-stub ...
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Sphingomonadaceae
Sphingomonadaceae are a gram-negative bacterial family of the Alphaproteobacteria. An important feature is the presence of sphingolipids (mainly 2′-hydroxymyristol dihydrosphingosine 1-glucuronic acid, "SGL-1") in the outer membrane of the cell wall. The cells are ovoid or rod-shaped. Others are also pleomorphic, i.e. the cells change the shape over time. Some species from Sphingomonadaceae family are dominant components of biofilms. Energy source While most species within Sphingomonadaceae family are heterotrophic, some are phototrophic. Function Some species of Sphingomonadaceae are known to degrade some aromatic compounds. This makes the bacteria of interest to environmental remediation. The diverse metabolic capacity of genera within the ''Sphingomonadaceae'' family, such as '' Sphingobium'', '' Novosphingobium'', and '' Sphingopyxis'' enable these genera to adapt to and be abundant in the presence of Bisphenol A. A microbial community with abundant ''Sphingomonadac ...
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Sphingomonas
''Sphingomonas'' was defined in 1990 as a group of Gram-negative, rod-shaped, chemoheterotrophic, strictly aerobic bacteria. They possess ubiquinone 10 as their major respiratory quinone, contain glycosphingolipids (GSLs), specifically ceramide, instead of lipopolysaccharide (LPS) in their cell envelopes, and typically produce yellow-pigmented colonies. The GSL serves to protect the bacteria from antibacterial substances. Unlike most Gram-negative bacteria, ''Sphingomonas'' carries endotoxins and has a hydrophobic surface characterized by the short nature of the GSL's carbohydrate portion. By 2001, the genus included more than 20 species that were quite diverse in terms of their phylogenetic, ecological, and physiological properties. As a result, ''Sphingomonas'' was subdivided into different genera: ''Sphingomonas'', ''Sphingobium'', '' Novosphingobium'', '' Sphingosinicella'', and '' Sphingopyxis''. These genera are commonly referred to collectively as sphingomonads. Distinct ...
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Pseudomonas
''Pseudomonas'' is a genus of Gram-negative, Gammaproteobacteria, belonging to the family Pseudomonadaceae and containing 191 described species. The members of the genus demonstrate a great deal of metabolic diversity and consequently are able to colonize a wide range of niches. Their ease of culture ''in vitro'' and availability of an increasing number of ''Pseudomonas'' strain genome sequences has made the genus an excellent focus for scientific research; the best studied species include '' P. aeruginosa'' in its role as an opportunistic human pathogen, the plant pathogen '' P. syringae'', the soil bacterium '' P. putida'', and the plant growth-promoting ''P. fluorescens, P. lini, P. migulae'', and ''P. graminis''. Because of their widespread occurrence in water and plant seeds such as dicots, the pseudomonads were observed early in the history of microbiology. The generic name ''Pseudomonas'' created for these organisms was defined in rather vague terms by Walter Migula ...
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Gellan Gum
Gellan gum is a water-soluble anionic polysaccharide produced by the bacterium '' Sphingomonas elodea'' (formerly ''Pseudomonas elodea'' based on the taxonomic classification at the time of its discovery). The gellan-producing bacterium was discovered and isolated by the former Kelco Division of Merck & Company, Inc. in 1978 from the lily plant tissue from a natural pond in Pennsylvania. It was initially identified as a substitute gelling agent at significantly lower use level to replace agar in solid culture media for the growth of various microorganisms. Its initial commercial product with the trademark as Gelrite gellan gum, was subsequently identified as a suitable agar substitute as gelling agent in various clinical bacteriological media. Chemical structure The repeating unit of the polymer is a tetrasaccharide, which consists of two residues of D-glucose and one of each residues of L-rhamnose and D-glucuronic acid. The tetrasaccharide repeat has the following structure: D-G ...
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Agar
Agar ( or ), or agar-agar, is a jelly-like substance consisting of polysaccharides obtained from the cell walls of some species of red algae, primarily from ogonori (''Gracilaria'') and "tengusa" (''Gelidiaceae''). As found in nature, agar is a mixture of two components, the linear polysaccharide agarose and a heterogeneous mixture of smaller molecules called agaropectin. It forms the supporting structure in the cell walls of certain species of algae and is released on boiling. These algae are known as agarophytes, belonging to the Rhodophyta (red algae) phylum. The processing of food-grade agar removes the agaropectin, and the commercial product is essentially pure agarose. Agar has been used as an ingredient in desserts throughout Asia and also as a solid substrate to contain culture media for microbiological work. Agar can be used as a laxative; an appetite suppressant; a vegan substitute for gelatin; a thickener for soups; in fruit preserves, ice cream, and other ...
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Maltodextrin
Maltodextrin is a polysaccharide that is used as a food ingredient. It is produced from vegetable starch by partial hydrolysis and is usually found as a white hygroscopic spray-dried powder. Maltodextrin is easily digestible, being absorbed as rapidly as glucose and may be either moderately sweet or almost flavorless (depending on the degree of polymerisation). It can be found as an ingredient in a variety of processed foods. Structure Maltodextrin consists of D-glucose units connected in chains of variable length. The glucose units are primarily linked with α(1→4) glycosidic bonds, like that seen in the linear derivative of glycogen (after the removal of α1,6- branching). Maltodextrin is typically composed of a mixture of chains that vary from three to 17 glucose units long. Maltodextrins are classified by DE ( dextrose equivalent) and have a DE between 3 and 20. The higher the DE value, the shorter the glucose chains, the higher the sweetness, the higher the solubility ...
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