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Spiral Plater
A spiral plater is an instrument used to dispense a liquid sample onto a Petri dish in a spiral pattern. Commonly used as part of a CFU count procedure for the purpose of determining the number of microbes in the sample.J. E. Gilchrist, J. E. Campbell, C. B. Donnelly, J. T. Peeler, and J. M. Delaney"Spiral Plate Method for Bacterial Determination" '' Appl Microbiol. '', 1973 February; 25(2): 244–252., In this setting, after spiral plating, the Petri dish is incubated for several hours after which the number of colony forming microbes (CFU) is determined. Spiral platers are also used for research, clinical diagnostics and as a method for covering a Petri dish with bacteria before placing antibiotic discs for AST. Mode of action The spiral plater rotates the dish while simultaneously dispensing the liquid and either linearly moving the dish or the dispensing tip. This creates the common spiral pattern. If all movements are done in constant speed, the spiral created would hav ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spiral Plater Pattern On Petri Dish
In mathematics, a spiral is a curve which emanates from a point, moving farther away as it revolves around the point. Helices Two major definitions of "spiral" in the American Heritage Dictionary are:Spiral ''American Heritage Dictionary of the English Language'', Houghton Mifflin Company, Fourth Edition, 2009. # a curve on a plane that winds around a fixed center point at a continuously increasing or decreasing distance from the point. # a three-dimensional curve that turns around an axis at a constant or continuously varying distance while moving parallel to the axis; a . The first definition describes a |
Petri Dish
A Petri dish (alternatively known as a Petri plate or cell-culture dish) is a shallow transparent lidded dish that biologists use to hold growth medium in which cells can be cultured,R. C. Dubey (2014): ''A Textbook Of Biotechnology For Class-XI'', 4th edition, p. 469. originally, cells of bacteria, fungi and small mosses. The container is named after its inventor, German bacteriologist Julius Richard Petri. It is the most common type of culture plate. The Petri dish is one of the most common items in biology laboratories and has entered popular culture. The term is sometimes written in lower case, especially in non-technical literature. What was later called Petri dish was originally developed by German physician Robert Koch in his private laboratory in 1881, as a precursor method. Petri, as assistant to Koch, at Berlin University made the final modifications in 1887 as used today. Penicillin, the first antibiotic, was discovered in 1929 when Alexander Fleming noticed that m ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spiral
In mathematics, a spiral is a curve which emanates from a point, moving farther away as it revolves around the point. Helices Two major definitions of "spiral" in the American Heritage Dictionary are:Spiral ''American Heritage Dictionary of the English Language'', Houghton Mifflin Company, Fourth Edition, 2009. # a curve on a plane that winds around a fixed center point at a continuously increasing or decreasing distance from the point. # a three-dimensional curve that turns around an axis at a constant or continuously varying distance while moving parallel to the axis; a . The first definition describes a |
Colony-forming Unit
In microbiology, colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions. Counting with colony-forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic examination which counts all cells, living or dead. The visual appearance of a colony in a cell culture requires significant growth, and when counting colonies, it is uncertain if the colony arose from one cell or a group of cells. Expressing results as colony-forming units reflects this uncertainty. Theory The purpose of plate counting is to estimate the number of cells present based on their ability to give rise to colonies under specific conditions of nutrient medium, temperature and time. Theoretically, one viable cell can give rise to a colony through replication. However, solitary cells are the exception in na ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Appl Microbiol
''Applied and Environmental Microbiology'' is a biweekly peer-reviewed scientific journal published by the American Society for Microbiology. It was established in 1953 as ''Applied Microbiology'' and obtained its current name in 1975. Articles older than six months are available free of cost from the website, however, the newly published articles within six months are available to subscribers only. According to the ''Journal Citation Reports'', the journal has a 2021 impact factor of 5.005. The journal has been ranked as one of the top 100 journals over the past 100 years in the fields of biology and medicine.Special Libraries Association100 Journals in last 100 years/ref> The editor-in-chief is Gemma Reguera (Michigan State University Michigan State University (Michigan State, MSU) is a public university, public Land-grant university, land-grant research university in East Lansing, Michigan. It was founded in 1855 as the Agricultural College of the State of Michigan, the fi . ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationsh ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Antibiotic Sensitivity
Antibiotic sensitivity testing or antibiotic susceptibility testing is the measurement of the susceptibility of bacteria to antibiotics. It is used because bacteria may have resistance to some antibiotics. Sensitivity testing results can allow a clinician to change the choice of antibiotics from empiric therapy, which is when an antibiotic is selected based on clinical suspicion about the site of an infection and common causative bacteria, to directed therapy, in which the choice of antibiotic is based on knowledge of the organism and its sensitivities. Sensitivity testing usually occurs in a medical laboratory, and uses culture methods that expose bacteria to antibiotics, or genetic methods that test to see if bacteria have genes that confer resistance. Culture methods often involve measuring the diameter of areas without bacterial growth, called zones of inhibition, around paper discs containing antibiotics on agar culture dishes that have been evenly inoculated with bacteria ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Linear Motion
Linear motion, also called rectilinear motion, is one-dimensional motion along a straight line, and can therefore be described mathematically using only one spatial dimension. The linear motion can be of two types: uniform linear motion, with constant velocity (zero acceleration); and non-uniform linear motion, with variable velocity (non-zero acceleration). The motion of a particle (a point-like object) along a line can be described by its position x, which varies with t (time). An example of linear motion is an athlete running a 100-meter dash along a straight track. Linear motion is the most basic of all motion. According to Newton's first law of motion, objects that do not experience any net force will continue to move in a straight line with a constant velocity until they are subjected to a net force. Under everyday circumstances, external forces such as gravity and friction can cause an object to change the direction of its motion, so that its motion cannot be described as ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Incubator (culture)
An incubator is a device used to grow and maintain microbiological cultures or cell cultures. The incubator maintains optimal temperature, humidity and other conditions such as the CO2 and oxygen content of the atmosphere inside. Incubators are essential for much experimental work in cell biology, microbiology and molecular biology and are used to culture both bacterial and eukaryotic cells. An incubator is made up of a chamber with a regulated temperature. Some incubators also regulate humidity, gas composition, or ventilation within that chamber. The simplest incubators are insulated boxes with an adjustable heater, typically going up to 60 to 65 °C (140 to 150 °F), though some can go slightly higher (generally to no more than 100 °C). The most commonly used temperature both for bacteria such as the frequently used E. coli as well as for mammalian cells is approximately 37 °C (99 °F), as these organisms grow well under such conditions. For other organisms used in biologi ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Colony Counter
In microbiology, colony-forming unit (CFU, cfu or Cfu) is a unit which estimates the number of microbial cells (bacteria, fungi, viruses etc.) in a sample that are viable, able to multiply via binary fission under the controlled conditions. Counting with colony-forming units requires culturing the microbes and counts only viable cells, in contrast with microscopic examination which counts all cells, living or dead. The visual appearance of a colony in a cell culture requires significant growth, and when counting colonies, it is uncertain if the colony arose from one cell or a group of cells. Expressing results as colony-forming units reflects this uncertainty. Theory The purpose of plate counting is to estimate the number of cells present based on their ability to give rise to colonies under specific conditions of nutrient medium, temperature and time. Theoretically, one viable cell can give rise to a colony through replication. However, solitary cells are the exception in na ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biological Techniques And Tools
Biology is the scientific study of life. It is a natural science with a broad scope but has several unifying themes that tie it together as a single, coherent field. For instance, all organisms are made up of cells that process hereditary information encoded in genes, which can be transmitted to future generations. Another major theme is evolution, which explains the unity and diversity of life. Energy processing is also important to life as it allows organisms to move, grow, and reproduce. Finally, all organisms are able to regulate their own internal environments. Biologists are able to study life at multiple levels of organization, from the molecular biology of a cell to the anatomy and physiology of plants and animals, and evolution of populations.Based on definition from: Hence, there are multiple subdisciplines within biology, each defined by the nature of their research questions and the tools that they use. Like other scientists, biologists use the scientific meth ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
