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Single Colour Reflectometry
Single colour reflectometry (SCORE), formerly known as imaging Reflectometric Interferometry (iRIf) and 1-lambda Reflectometry, is a physical method based on interference of monochromatic light at thin films, which is used to investigate (bio-)molecular interactions. The obtained binding curves using SCORE provide detailed information on kinetics and thermodynamics of the observed interaction(s) as well as on concentrations of the used analytes. These data can be relevant for pharmaceutical screening and drug design, biosensors and other biomedical applications, diagnostics, and cell-based assays. Principle The underlying principle corresponds to that of the Fabry-Pérot interferometer, which is also the underlying principle for the white-light interferometry. Realisation / setup Monochromatic light is illuminated vertically on the rear side of a transparent multi-layer substrate. The partial beams of the monochromatic light are transmitted and reflected at each interph ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Receptor–ligand Kinetics
In biochemistry, receptor–ligand kinetics is a branch of chemical kinetics in which the kinetic species are defined by different non-covalent bindings and/or conformations of the molecules involved, which are denoted as ''receptor (biochemistry), receptor(s)'' and ''ligand (biochemistry), ligand(s)''. Receptor–ligand binding kinetics also involves the on- and off-rates of binding. A main goal of receptor–ligand kinetics is to determine the concentrations of the various kinetic species (i.e., the states of the receptor and ligand) at all times, from a given set of initial concentrations and a given set of rate constants. In a few cases, an analytical solution of the rate equations may be determined, but this is relatively rare. However, most rate equations can be integrated numerically, or approximately, using the steady state (chemistry), steady-state approximation. A less ambitious goal is to determine the final ''equilibrium'' concentrations of the kinetic species, which i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bio-layer Interferometry
Bio-layer interferometry (BLI) is an optical biosensing technology that analyzes biomolecular interactions in real-time without the need for fluorescent labeling. Alongside Surface Plasmon Resonance, BLI is one of few widely available label-free biosensing technologies, a detection style that yields more information in less time than traditional processes. The technology relies on the phase shift-wavelength correlation created between interference patterns off of two unique surfaces on the tip of a biosensor. BLI has significant applications in quantifying binding strength, measuring protein interactions, and identifying properties of reaction kinetics, such as rate constants and reaction rates. Method Mechanism Overview Bio-layer interferometry measures kinetics and biomolecular interactions on a basis of wave interference. To prepare for BLI analysis between two unique biomolecules, the ligand is first immobilized onto a bio compatible biosensor while the analyte is in solut ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Nanotechnology
Nanotechnology, also shortened to nanotech, is the use of matter on an atomic, molecular, and supramolecular scale for industrial purposes. The earliest, widespread description of nanotechnology referred to the particular technological goal of precisely manipulating atoms and molecules for fabrication of macroscale products, also now referred to as molecular nanotechnology. A more generalized description of nanotechnology was subsequently established by the National Nanotechnology Initiative, which defined nanotechnology as the manipulation of matter with at least one dimension sized from 1 to 100 nanometers (nm). This definition reflects the fact that quantum mechanical effects are important at this quantum-realm scale, and so the definition shifted from a particular technological goal to a research category inclusive of all types of research and technologies that deal with the special properties of matter which occur below the given size threshold. It is therefore common to ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Forensic Techniques
Forensic science, also known as criminalistics, is the application of science to criminal and civil laws, mainly—on the criminal side—during criminal investigation, as governed by the legal standards of admissible evidence and criminal procedure. Forensic science is a broad field that includes; DNA analysis, fingerprint analysis, blood stain pattern analysis, firearms examination and ballistics, tool mark analysis, serology, toxicology, hair and fiber analysis, entomology, questioned documents, anthropology, odontology, pathology, epidemiology, footwear and tire tread analysis, drug chemistry, paint and glass analysis, digital audio video and photo analysis. Forensic scientists collect, preserve, and analyze scientific evidence during the course of an investigation. While some forensic scientists travel to the scene of the crime to collect the evidence themselves, others occupy a laboratory role, performing analysis on objects brought to them by other individuals. Still ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Biophysics
Biophysics is an interdisciplinary science that applies approaches and methods traditionally used in physics to study biological phenomena. Biophysics covers all scales of biological organization, from molecular to organismic and populations. Biophysical research shares significant overlap with biochemistry, molecular biology, physical chemistry, physiology, nanotechnology, bioengineering, computational biology, biomechanics, developmental biology and systems biology. The term ''biophysics'' was originally introduced by Karl Pearson in 1892.Roland Glaser. Biophysics: An Introduction'. Springer; 23 April 2012. . The term ''biophysics'' is also regularly used in academia to indicate the study of the physical quantities (e.g. electric current, temperature, stress, entropy) in biological systems. Other biological sciences also perform research on the biophysical properties of living organisms including molecular biology, cell biology, chemical biology, and biochemistry. Overview ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spectroscopy
Spectroscopy is the field of study that measures and interprets the electromagnetic spectra that result from the interaction between electromagnetic radiation and matter as a function of the wavelength or frequency of the radiation. Matter waves and acoustic waves can also be considered forms of radiative energy, and recently gravitational waves have been associated with a spectral signature in the context of the Laser Interferometer Gravitational-Wave Observatory (LIGO) In simpler terms, spectroscopy is the precise study of color as generalized from visible light to all bands of the electromagnetic spectrum. Historically, spectroscopy originated as the study of the wavelength dependence of the absorption by gas phase matter of visible light dispersed by a prism. Spectroscopy, primarily in the electromagnetic spectrum, is a fundamental exploratory tool in the fields of astronomy, chemistry, materials science, and physics, allowing the composition, physical structure and e ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Bio-layer Interferometry
Bio-layer interferometry (BLI) is an optical biosensing technology that analyzes biomolecular interactions in real-time without the need for fluorescent labeling. Alongside Surface Plasmon Resonance, BLI is one of few widely available label-free biosensing technologies, a detection style that yields more information in less time than traditional processes. The technology relies on the phase shift-wavelength correlation created between interference patterns off of two unique surfaces on the tip of a biosensor. BLI has significant applications in quantifying binding strength, measuring protein interactions, and identifying properties of reaction kinetics, such as rate constants and reaction rates. Method Mechanism Overview Bio-layer interferometry measures kinetics and biomolecular interactions on a basis of wave interference. To prepare for BLI analysis between two unique biomolecules, the ligand is first immobilized onto a bio compatible biosensor while the analyte is in solut ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Tübingen
Tübingen (, , Swabian: ''Dibenga'') is a traditional university city in central Baden-Württemberg, Germany. It is situated south of the state capital, Stuttgart, and developed on both sides of the Neckar and Ammer rivers. about one in three of the 90,000 people living in Tübingen is a student. As of the 2018/2019 winter semester, 27,665 students attend the Eberhard Karls University of Tübingen. The city has the lowest median age in Germany, in part due to its status as a university city. As of December 31, 2015, the average age of a citizen of Tübingen is 39.1 years. The city is known for its veganism and environmentalism. Immediately north of the city lies the Schönbuch, a densely wooded nature park. The Swabian Alb mountains rise about (beeline Tübingen City to Roßberg - 869 m) to the southeast of Tübingen. The Ammer and Steinlach rivers are tributaries of the Neckar river, which flows in an easterly direction through the city, just south of the medieval old t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Fluorescence
Fluorescence is the emission of light by a substance that has absorbed light or other electromagnetic radiation. It is a form of luminescence. In most cases, the emitted light has a longer wavelength, and therefore a lower photon energy, than the absorbed radiation. A perceptible example of fluorescence occurs when the absorbed radiation is in the ultraviolet region of the electromagnetic spectrum (invisible to the human eye), while the emitted light is in the visible region; this gives the fluorescent substance a distinct color that can only be seen when the substance has been exposed to UV light. Fluorescent materials cease to glow nearly immediately when the radiation source stops, unlike phosphorescent materials, which continue to emit light for some time after. Fluorescence has many practical applications, including mineralogy, gemology, medicine, chemical sensors (fluorescence spectroscopy), fluorescent labelling, dyes, biological detectors, cosmic-ray detection, vacu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
SCORE Multiplex
Score or scorer may refer to: *Test score, the result of an exam or test Business * Score Digital, now part of Bauer Radio * Score Entertainment, a former American trading card design and manufacturing company * Score Media, a former Canadian media company Mathematics *Score (statistics), a quantity in statistics *Score, a quantity of twenty ( 20) units *Raw score, an original datum that has not been transformed *Score test, a statistical test * Scorer's function, solutions to differential equations *Scoring rule, measuring the accuracy of probabilistic predictions *Standard score, a quantity derived from the raw score * Score, a period of 20 years Science and technology *Single colour reflectometry (SCORE), an optical technique for monitoring biomolecular interactions Arts, entertainment, and media *Event score, written or printed instructions for a visual art performance Films * ''Score'' (1974 film), an American adult film * ''Score'' (2016 film), a documentary * '' Sco ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Coherence Length
In physics, coherence length is the propagation distance over which a coherent wave (e.g. an electromagnetic wave) maintains a specified degree of coherence. Wave interference is strong when the paths taken by all of the interfering waves differ by less than the coherence length. A wave with a longer coherence length is closer to a perfect sinusoidal wave. Coherence length is important in holography and telecommunications engineering. This article focuses on the coherence of classical electromagnetic fields. In quantum mechanics, there is a mathematically analogous concept of the quantum coherence length of a wave function. Formulas In radio-band systems, the coherence length is approximated by :L = \frac \approx \frac ~, where \, c \, is the speed of light in a vacuum, \, n \, is the refractive index of the medium, and \, \mathrm f \, is the bandwidth of the source or \, \lambda \, is the signal wavelength and \, \Delta \lambda \, is the width of the range of wavelengths i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
