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Romanowsky Stain
Romanowsky staining, also known as Romanowsky–Giemsa staining, is a prototypical staining technique that was the forerunner of several distinct but similar stains widely used in hematology (the study of blood) and cytopathology (the study of diseased cells). Romanowsky-type stains are used to differentiate cells for microscopic examination in pathological specimens, especially blood and bone marrow films, and to detect parasites such as malaria within the blood. Stains that are related to or derived from the Romanowsky-type stains include Giemsa, Jenner, Wright, Field, May–Grünwald and Leishman stains. The staining technique is named after the Russian physician Dmitri Leonidovich Romanowsky (1861–1921), who was one of the first to recognize its potential for use as a blood stain. Mechanism The value of Romanowsky staining lies in its ability to produce a wide range of hues, allowing cellular components to be easily differentiated. This phenomenon is referred to as the '' ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Plasmodium Vivax 01
''Plasmodium'' is a genus of unicellular eukaryotes that are obligate parasites of vertebrates and insects. The life cycles of ''Plasmodium'' species involve development in a blood-feeding insect host which then injects parasites into a vertebrate host during a blood meal. Parasites grow within a vertebrate body tissue (often the liver) before entering the bloodstream to infect red blood cells. The ensuing destruction of host red blood cells can result in malaria. During this infection, some parasites are picked up by a blood-feeding insect (mosquitoes in majority cases), continuing the life cycle. ''Plasmodium'' is a member of the phylum Apicomplexa, a large group of parasitic eukaryotes. Within Apicomplexa, ''Plasmodium'' is in the order Haemosporida and family Plasmodiidae. Over 200 species of ''Plasmodium'' have been described, many of which have been subdivided into 14 subgenera based on parasite morphology and host range. Evolutionary relationships among different ''P ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
William Leishman
Lieutenant-General Sir William Boog Leishman, (, 6 November 1865 – 2 June 1926) was a Scottish pathologist and British Army medical officer. He was Director-General of Army Medical Services from 1923 to 1926. Biography Leishman was born in Glasgow and attended Westminster School and the University of Glasgow and entered the Royal Army Medical Corps. He served in India, where he did research on enteric fever and kala-azar. He returned to the United Kingdom and was stationed at the Victoria Hospital in Netley in 1897. In 1900 he was made Assistant Professor of Pathology in the Army Medical School, and described a method of staining blood for malaria and other parasites—a modification and simplification of the existing Romanowsky method using a compound of methylene blue and eosin, which became known as Leishman's stain. In 1901, while examining pathologic specimens of a spleen from a patient who had died of kala azar (now called "visceral leishmaniasis"), he observed oval ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Methanol
Methanol (also called methyl alcohol and wood spirit, amongst other names) is an organic chemical and the simplest aliphatic alcohol, with the formula C H3 O H (a methyl group linked to a hydroxyl group, often abbreviated as MeOH). It is a light, volatile, colourless, flammable liquid with a distinctive alcoholic odour similar to that of ethanol (potable alcohol). A polar solvent, methanol acquired the name wood alcohol because it was once produced chiefly by the destructive distillation of wood. Today, methanol is mainly produced industrially by hydrogenation of carbon monoxide. Methanol consists of a methyl group linked to a polar hydroxyl group. With more than 20 million tons produced annually, it is used as a precursor to other commodity chemicals, including formaldehyde, acetic acid, methyl tert-butyl ether, methyl benzoate, anisole, peroxyacids, as well as a host of more specialised chemicals. Occurrence Small amounts of methanol are present in normal, healthy hu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
James Homer Wright
James Homer Wright (April 8, 1869 – January 3, 1928) was an early and influential American pathologist, who was chief of pathology at Massachusetts General Hospital from 1896 to 1926. Wright was born in Pittsburgh, Pennsylvania. In 1915, he joined with Richard C. Cabot to begin publication of the ''Case Records of the Massachusetts General Hospital''. These began regular publication as the ''Boston Medical and Surgical Journal'' which later became the ''New England Journal of Medicine''. In 1924, Wright, along with Frank B. Mallory, published ''Pathological Technique: A Practical Manual for the Pathological Laboratory''. The book saw eight editions and for many years was the standard textbook in the field. He is the Wright in Wright's stain, and the Homer Wright rosettes associated with neuroblastoma. See also *Romanowsky stain Romanowsky staining, also known as Romanowsky–Giemsa staining, is a prototypical staining technique that was the forerunner of several disti ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Thionine
Thionine, also known as Lauth's violet, is the salt of a heterocyclic compound A heterocyclic compound or ring structure is a cyclic compound that has atoms of at least two different elements as members of its ring(s). Heterocyclic chemistry is the branch of organic chemistry dealing with the synthesis, properties, and .... It was firstly synthesised by Charles Lauth. A variety of salts are known including the chloride and acetate, called respectively thionine chloride and thionine acetate. The dye is structurally related to methylene blue, which also features a phenothiazine core. The dye's name is frequently misspelled with omission of the e, and is not to be confused with the plant protein thionin. The -ine ending indicates that the compound is an amine. Dye properties and use Thionine is a strongly staining metachromatic dye, which is widely used for histologic stain, biological staining. Thionine can also be used in place of Schiff reagent in quantitative Feulgen st ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Azure A
Azure A is an organic compound with the chemical formula C14H14ClN3S. It is a light blue to dark blue dye. It is used as a screening test for mucopolysaccharides. It can also be used to stain lysosome in blood smears, and is often used in Giemsa stain Giemsa stain (), named after German chemist and bacteriologist Gustav Giemsa, is a nucleic acid stain used in cytogenetics and for the histopathological diagnosis of malaria and other parasites. Uses It is specific for the phosphate groups of .... Thiazine dyes Chlorides Phenothiazines {{organic-compound-stub ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Demethylation
Demethylation is the chemical process resulting in the removal of a methyl group (CH3) from a molecule. A common way of demethylation is the replacement of a methyl group by a hydrogen atom, resulting in a net loss of one carbon and two hydrogen atoms. The counterpart of demethylation is methylation. In biochemistry In biochemical systems, the process of demethylation is catalyzed by demethylases. These enzymes oxidize N-methyl groups, which occur in histones and some forms of DNA: :R2N-CH3 + O → R2N-H + CH2O One such oxidative enzyme family is the cytochrome P450. Alpha-ketoglutarate-dependent hydroxylases are active for demethylation of DNA, operating by a similar pathway. These reactions exploit the weak C-H bond adjacent to amines. In particular, 5-methylcytosines in DNA can be demethylated by TET enzymes as illustrated in the figure. TET enzymes are dioxygenases in the family of alpha-ketoglutarate-dependent hydroxylases. A TET enzyme is an alpha-ketoglut ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Oxidative
Redox (reduction–oxidation, , ) is a type of chemical reaction in which the oxidation states of substrate change. Oxidation is the loss of electrons or an increase in the oxidation state, while reduction is the gain of electrons or a decrease in the oxidation state. There are two classes of redox reactions: * ''Electron-transfer'' – Only one (usually) electron flows from the reducing agent to the oxidant. This type of redox reaction is often discussed in terms of redox couples and electrode potentials. * ''Atom transfer'' – An atom transfers from one substrate to another. For example, in the rusting of iron, the oxidation state of iron atoms increases as the iron converts to an oxide, and simultaneously the oxidation state of oxygen decreases as it accepts electrons released by the iron. Although oxidation reactions are commonly associated with the formation of oxides, other chemical species can serve the same function. In hydrogenation, C=C (and other) bonds ar ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Azure B
Azure may refer to: Colour * Azure (color), a hue of blue ** Azure (heraldry) ** Shades of azure, shades and variations Arts and media * ''Azure'' (Art Farmer and Fritz Pauer album), 1987 * Azure (Gary Peacock and Marilyn Crispell album), 2013 * ''Azure'' (design magazine), Toronto, Ontario * Azure (heraldry), a blue tincture on flags or coats of arms * ''Azure'' (magazine), a periodical on Jewish thought and identity * ''Azure'' (painting), by Gustave Van de Woestijne * "Azure" (song), by Duke Ellington * "Azure", a song by the 3rd and the Mortal from the album ''Painting on Glass'' Computing * Microsoft Azure, a cloud computing platform * Mozilla Azure, a graphics abstraction API Places * Azure, Alberta, a locality in Canada * Azure, Montana, a census-designated place in the United States * Azure Window, a former natural arch in Malta Other uses * Azure Ryder (born 1996), an Australian singer, songwriter, and musician * Azure (barley), a malting barley variety * Bent ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cytoplasm
In cell biology, the cytoplasm is all of the material within a eukaryotic cell, enclosed by the cell membrane, except for the cell nucleus. The material inside the nucleus and contained within the nuclear membrane is termed the nucleoplasm. The main components of the cytoplasm are cytosol (a gel-like substance), the organelles (the cell's internal sub-structures), and various cytoplasmic inclusions. The cytoplasm is about 80% water and is usually colorless. The submicroscopic ground cell substance or cytoplasmic matrix which remains after exclusion of the cell organelles and particles is groundplasm. It is the hyaloplasm of light microscopy, a highly complex, polyphasic system in which all resolvable cytoplasmic elements are suspended, including the larger organelles such as the ribosomes, mitochondria, the plant plastids, lipid droplets, and vacuoles. Most cellular activities take place within the cytoplasm, such as many metabolic pathways including glycolysis, and proces ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chromatin
Chromatin is a complex of DNA and protein found in eukaryotic cells. The primary function is to package long DNA molecules into more compact, denser structures. This prevents the strands from becoming tangled and also plays important roles in reinforcing the DNA during cell division, preventing DNA damage, and regulating gene expression and DNA replication. During mitosis and meiosis, chromatin facilitates proper segregation of the chromosomes in anaphase; the characteristic shapes of chromosomes visible during this stage are the result of DNA being coiled into highly condensed chromatin. The primary protein components of chromatin are histones. An octamer of two sets of four histone cores (Histone H2A, Histone H2B, Histone H3, and Histone H4) bind to DNA and function as "anchors" around which the strands are wound.Maeshima, K., Ide, S., & Babokhov, M. (2019). Dynamic chromatin organization without the 30-nm fiber. ''Current opinion in cell biology, 58,'' 95–104. https://doi.o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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