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Phase Contrast
Phase-contrast imaging is a method of imaging that has a range of different applications. It measures differences in the refractive index of different materials to differentiate between structures under analysis. In conventional light microscopy, phase contrast can be employed to distinguish between structures of similar transparency, and to examine crystals on the basis of their double refraction. This has uses in biological, medical and geological science. In X-ray tomography, the same physical principles can be used to increase image contrast by highlighting small details of differing refractive index within structures that are otherwise uniform. In transmission electron microscopy (TEM), phase contrast enables very high resolution (HR) imaging, making it possible to distinguish features a few Angstrom apart (at this point highest resolution is 40 pm). Atomic physics Phase-contrast imaging is commonly used in atomic physics to describe a range of techniques for dispersively i ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Image
An image or picture is a visual representation. An image can be Two-dimensional space, two-dimensional, such as a drawing, painting, or photograph, or Three-dimensional space, three-dimensional, such as a carving or sculpture. Images may be displayed through other media, including a Projector, projection on a surface, activation of electronic signals, or Display device, digital displays; they can also be reproduced through mechanical means, such as photography, printmaking, or Photocopier, photocopying. Images can also be Animation, animated through digital or physical processes. In the context of signal processing, an image is a distributed amplitude of color(s). In optics, the term ''image'' (or ''optical image'') refers specifically to the reproduction of an object formed by light waves coming from the object. A ''volatile image'' exists or is perceived only for a short period. This may be a reflection of an object by a mirror, a projection of a camera obscura, or a scene d ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Atomic Electron Transition
In atomic physics and chemistry, an atomic electron transition (also called an atomic transition, quantum jump, or quantum leap) is an electron changing from one energy level to another within an atom or artificial atom. The time scale of a quantum jump has not been measured experimentally. However, the Franck–Condon principle binds the upper limit of this parameter to the order of attoseconds. Electrons can ''relax'' into states of lower energy by emitting electromagnetic radiation in the form of a photon. Electrons can also absorb passing photons, which ''excites'' the electron into a state of higher energy. The larger the energy separation between the electron's initial and final state, the shorter the photons' wavelength. History Danish physicist Niels Bohr first theorized that electrons can perform quantum jumps in 1913. Soon after, James Franck and Gustav Ludwig Hertz proved experimentally that atoms have quantized energy states. The observability of quantum jum ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Interferometry
Interferometry is a technique which uses the ''interference (wave propagation), interference'' of Superposition principle, superimposed waves to extract information. Interferometry typically uses electromagnetic waves and is an important investigative technique in the fields of astronomy, Optical fiber, fiber optics, engineering metrology, optical metrology, oceanography, seismology, spectroscopy (and its applications to chemistry), quantum mechanics, Nuclear physics, nuclear and particle physics, plasma physics, interactome, biomolecular interactions, surface profiling, microfluidics, mechanical stress/strain measurement, velocimetry, optometry, and making holograms. Interferometers are devices that extract information from interference. They are widely used in science and industry for the measurement of microscopic displacements, refractive index changes and surface irregularities. In the case with most interferometers, light from a single source is split into two beams that ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Diffraction Grating
In optics, a diffraction grating is an optical grating with a periodic structure that diffraction, diffracts light, or another type of electromagnetic radiation, into several beams traveling in different directions (i.e., different diffraction angles). The emerging coloration is a form of structural coloration. The directions or diffraction angles of these beams depend on the wave (light) Angle of incidence (optics), incident angle to the diffraction grating, the spacing or periodic distance between adjacent diffracting elements (e.g., parallel slits for a transmission grating) on the grating, and the wavelength of the incident light. The grating acts as a dispersion (optics), dispersive element. Because of this, diffraction gratings are commonly used in monochromators and spectrometers, but other applications are also possible such as optical encoders for high-precision motion control and wavefront measurement. For typical applications, a reflection (optics), reflective grati ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Talbot Effect
The Talbot effect is a diffraction effect first observed in 1836 by Henry Fox Talbot. When a plane wave is incident upon a periodic diffraction grating, the image of the grating is repeated at regular distances away from the grating plane. The regular distance is called the Talbot length, and the repeated images are called self images or Talbot images. Furthermore, at half the Talbot length, a self-image also occurs, but phase-shifted by half a period (the physical meaning of this is that it is laterally shifted by half the width of the grating period). At smaller regular fractions of the Talbot length, sub-images can also be observed. At one quarter of the Talbot length, the self-image is halved in size, and appears with half the period of the grating (thus twice as many images are seen). At one eighth of the Talbot length, the period and size of the images is halved again, and so forth creating a fractal pattern of sub images with ever-decreasing size, often referred to as a Talb ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Wave Propagation
In physics, mathematics, engineering, and related fields, a wave is a propagating dynamic disturbance (change from equilibrium) of one or more quantities. '' Periodic waves'' oscillate repeatedly about an equilibrium (resting) value at some frequency. When the entire waveform moves in one direction, it is said to be a travelling wave; by contrast, a pair of superimposed periodic waves traveling in opposite directions makes a '' standing wave''. In a standing wave, the amplitude of vibration has nulls at some positions where the wave amplitude appears smaller or even zero. There are two types of waves that are most commonly studied in classical physics: mechanical waves and electromagnetic waves. In a mechanical wave, stress and strain fields oscillate about a mechanical equilibrium. A mechanical wave is a local deformation (strain) in some physical medium that propagates from particle to particle by creating local stresses that cause strain in neighboring particles too ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
X-ray Detector
X-ray detectors are devices used to measure the flux, spatial distribution, spectrum, and/or other properties of X-rays. Detectors can be divided into two major categories: imaging detectors (such as photographic plates and X-ray film (photographic film), now mostly replaced by various digitizing devices like image plates or flat panel detectors) and dose measurement devices (such as ionization chambers, Geiger counters, and dosimeters used to measure the local radiation exposure, dose, and/or dose rate, for example, for verifying that radiation protection equipment and procedures are effective on an ongoing basis). X-ray imaging To obtain an image with any type of image detector the part of the patient to be X-rayed is placed between the X-ray source and the image receptor to produce a shadow of the internal structure of that particular part of the body. X-rays are partially blocked ("attenuated") by dense tissues such as bone, and pass more easily through soft tissues. Are ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phase-contrast X-ray Image Of Spider
__NOTOC__ Phase-contrast microscopy (PCM) is an optical microscopy technique that converts phase shifts in light passing through a transparent specimen to brightness changes in the image. Phase shifts themselves are invisible, but become visible when shown as brightness variations. When light waves travel through a medium other than a vacuum, interaction with the medium causes the wave amplitude and phase (waves), phase to change in a manner dependent on properties of the medium. Changes in amplitude (brightness) arise from the scattering and absorption of light, which is often wavelength-dependent and may give rise to colors. Photographic equipment and the human eye are only sensitive to amplitude variations. Without special arrangements, phase changes are therefore invisible. Yet, phase changes often convey important information. Phase-contrast microscopy is particularly important in biology. It reveals many cell (biology), cellular structures that are invisible with a brigh ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Optic Axis Of A Crystal
An optic axis of a crystal is a direction in which a ray of transmitted light suffers no birefringence (double refraction). An optic axis is a direction rather than a single line: all rays that are parallel to that direction exhibit the same lack of birefringence. Crystals may have a single optic axis, in which case they are ''uniaxial'', or two different optic axes, in which case they are ''biaxial''. Non-crystalline materials generally have no birefringence and thus, no optic axis. A uniaxial crystal (e.g. calcite, quartz) is isotropic within the plane orthogonal to the optic axis of the crystal. Explanation The internal structure of crystals (the specific structure of the crystal lattice, and the specific atoms or molecules of which it is composed) causes the speed of light in the material, and therefore the material's refractive index, to depend on both the light's direction of propagation and its polarization. The dependence on polarization causes birefringence, in which two ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Staining
Staining is a technique used to enhance contrast in samples, generally at the Microscope, microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissue (biology), tissues), in cytology (microscopic study of cell (biology), cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell (biology), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Phase Plate
Phase or phases may refer to: Science *State of matter, or phase, one of the distinct forms in which matter can exist *Phase (matter), a region of space throughout which all physical properties are essentially uniform *Phase space, a mathematical space in which each possible state of a physical system is represented by a point also referred to as a "microscopic state" **Phase space formulation, a formulation of quantum mechanics in phase space *Phase (waves), the position of a point in time (an instant) on a waveform cycle **Instantaneous phase, generalization for both cyclic and non-cyclic phenomena *AC phase, the phase offset between alternating current electric power in multiple conducting wires **Single-phase electric power, distribution of AC electric power in a system where the voltages of the supply vary in unison **Three-phase electric power, a common method of AC electric power generation, transmission, and distribution *Phase problem, the loss of information (the phase) ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
