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Orthochromasia
In chemistry, orthochromasia is the property of a dye or stain to not change color on binding to a target, as opposed to metachromatic stains, which change color. The word is derived from the Greek '' orthos'' (correct, upright), and chromatic (color). Toluidine blue is an example of a partially orthochromatic dye, as it stains nucleic acids by its orthochromatic color (blue), but stains mast cell granules in its metachromatic color (red). In spectral terms, orthochromasia refers to maintaining the position of spectral peaks, while metachromasia refers to a shift in wavelength, becoming either shorter or longer. In photography, an orthochromatic light spectrum is one devoid of red light. In biology, orthochromatic refers to the greyish staining because of acidophilic and basophilic mixture in the cell. Orthochromatic photography Orthochromatic photography refers to a photographic emulsion that is sensitive to only blue and green light, and thus can be processed with a red ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Panchromatic
Panchromatic emulsion is a type of black-and-white photographic emulsion that is sensitive to all wavelengths of visible light. Description A panchromatic emulsion renders a realistic reproduction of a scene as it appears to the human eye, although with no colors. Almost all modern photographic film is panchromatic. Some older types of film were orthochromatic and were not sensitive to certain wavelengths of light. As naturally prepared, a silver halide photographic emulsion is much more sensitive to blue and UV light than to green and red wavelengths. The German chemist Hermann W. Vogel found out how to extend the sensitivity into the green, and later the orange, by adding sensitising dyes to the emulsion. By the addition of erythrosine the emulsion could be made orthochromatic while some cyanine derivatives confer sensitivity to the whole visible spectrum making it panchromatic. However, his technique was not extended to achieve a fully panchromatic film until the early 190 ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Panchromatic Film
Panchromatic emulsion is a type of black-and-white photographic emulsion that is sensitive to all wavelengths of visible light. Description A panchromatic emulsion renders a realistic reproduction of a scene as it appears to the human eye, although with no colors. Almost all modern photographic film is panchromatic. Some older types of film were orthochromatic and were not sensitive to certain wavelengths of light. As naturally prepared, a silver halide photographic emulsion is much more sensitive to blue and UV light than to green and red wavelengths. The German chemist Hermann W. Vogel found out how to extend the sensitivity into the green, and later the orange, by adding sensitising dyes to the emulsion. By the addition of erythrosine the emulsion could be made orthochromatic while some cyanine derivatives confer sensitivity to the whole visible spectrum making it panchromatic. However, his technique was not extended to achieve a fully panchromatic film until the early 1900s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Photographic Emulsion
Photographic emulsion is a light-sensitive colloid used in film-based photography. Most commonly, in silver-gelatin photography, it consists of silver halide crystals dispersed in gelatin. The emulsion is usually coated onto a substrate of glass, films (of cellulose nitrate, cellulose acetate or polyester), paper, or fabric. Photographic emulsion is not a true emulsion, but a suspension of solid particles (silver halide) in a fluid (gelatin in solution). However, the word ''emulsion'' is customarily used in a photographic context. Gelatin or gum arabic layers sensitized with dichromate used in the dichromated colloid processes carbon and gum bichromate are sometimes called ''emulsions''. Some processes do not have emulsions, such as platinum, cyanotype, salted paper, or kallitype. Components Photographic emulsion is a fine suspension of insoluble light-sensitive crystals in a colloid sol, usually consisting of gelatin. The light-sensitive component is one or a mixture o ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Comparison Between Orthochromatic And Panchromatic Film
Comparison or comparing is the act of evaluating two or more things by determining the relevant, comparable characteristics of each thing, and then determining which characteristics of each are similar to the other, which are different, and to what degree. Where characteristics are different, the differences may then be evaluated to determine which thing is best suited for a particular purpose. The description of similarities and differences found between the two things is also called a comparison. Comparison can take many distinct forms, varying by field: To compare things, they must have characteristics that are similar enough in relevant ways to merit comparison. If two things are too different to compare in a useful way, an attempt to compare them is colloquially referred to in English as "comparing apples and oranges." Comparison is widely used in society, in science and in the arts. General usage Comparison is a natural activity, which even animals engage in when deci ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Erythrosine
Erythrosine, also known as Red No. 3, is an organoiodine compound, specifically a derivative of fluorone. It is a pink dye which is primarily used for food coloring. It is the disodium salt of 2,4,5,7-tetraiodofluorescein. Its maximum absorbance is at 530 nm in an aqueous solution, and it is subject to photodegradation. Uses It is used as a: * food coloring * printing ink * biological stain * dental plaque disclosing agent * radiopaque medium * sensitizer for orthochromatic photographic films *Visible light photoredox catalyst Erythrosine is commonly used in sweets such as some candies and popsicles, and even more widely used in cake-decorating gels. It is also used to color pistachio shells. As a food additive, it has the E number E127. Health effects As a result of efforts begun in the 1970s, in 1990 the U.S. FDA had instituted a partial ban on erythrosine, citing research that high doses have been found to cause cancer in rats. A 1990 study concluded that "chr ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Josef Maria Eder
Josef Maria Eder (16 March 1855 – 18 October 1944) was an Austrian chemist who specialized in the chemistry of photography, and who wrote a comprehensive early history of the technical development of chemical photography. Life and work Eder was born in Krems an der Donau in 1855. He studied chemistry, physics and mathematics at the Vienna University of Technology and at the University of Vienna. In 1876, he received his PhD and in 1879, after his habilitation, became lecturer at the Vienna University of Technology. His research then was focused on the chemistry of photography. After spending some time at the Staatliche Gewerbeschule Vienna, he became lecturer at the Höhere Gewerbeschule Vienna. This change improved his possibilities for doing research. In the following years, Eder developed sensitized gelatin silver process. Orthochromatic photographic plates, in combination with a color filter counter-acting the plates' inhomogeneous sensitivity to light of different wavelength ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Aniline
Aniline is an organic compound with the formula C6 H5 NH2. Consisting of a phenyl group attached to an amino group, aniline is the simplest aromatic amine In organic chemistry, an aromatic amine is an organic compound consisting of an aromatic ring attached to an amine. It is a broad class of compounds that encompasses aniline Aniline is an organic compound with the formula C6 H5 NH2. Consi .... It is an industrially significant Commodity chemicals, commodity chemical, as well as a versatile starting material for fine chemical synthesis. Its main use is in the manufacture of precursors to polyurethane, dyes, and other industrial chemicals. Like most volatile amines, it has the odor of rotten fish. It Combustion, ignites readily, burning with a smoky flame characteristic of aromatic compounds. It is toxic to humans. Relative to benzene, it is electron-rich. It thus participates more rapidly in electrophilic aromatic substitution reactions. Likewise, it is also prone ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Hermann Wilhelm Vogel
Hermann Wilhelm Vogel (26 March 1834 – 17 December 1898) was a German photochemist and photographer who discovered dye sensitization, which is of great importance to photography. Academic career After finishing school in Frankfurt (Oder), he studied at the Royal Industrial Institute of Berlin, earning his Ph.D. with Karl Friedrich August Rammelsberg in 1863. Vogel's thesis, which was published in ''Poggendorffs Annalen'' , had the title: ''Über das Verhalten des Chlorsilbers, Bromsilbers und Iodsilbers im Licht und die Theorie der Photographie'' (Reactions of Silver Chloride, Silver Bromide and Silver Iodide with Light and the Theory of Photography). This marked the beginning of his research into the photographic process. From 1860 until 1865, he was an assistant in the mineralogical museum of the University of Berlin, and from 1884 was director of the photo-technical laboratory of the Technical Institute there. From 1864 he was a professor at Berlin's Technische Hochschu ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cyan
Cyan () is the color between green and blue on the visible spectrum of light. It is evoked by light with a predominant wavelength between 490 and 520 nm, between the wavelengths of green and blue. In the subtractive color system, or CMYK color model, which can be overlaid to produce all colors in paint and color printing, cyan is one of the primary colors, along with magenta and yellow. In the additive color system, or RGB color model, used to create all the colors on a computer or television display, cyan is made by mixing equal amounts of green and blue light. Cyan is the complement of red; it can be made by the removal of red from white. Mixing red light and cyan light at the right intensity will make white light. Colors in the cyan color range are teal, turquoise, electric blue, aquamarine, and others described as blue-green. Etymology and terminology Its name is derived from the Ancient Greek word ''kyanos'' (κύανος), meaning "dark blue enamel, Lapis lazuli". It ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Safelight
A safelight is a light source suitable for use in a photographic darkroom. It provides illumination only from parts of the visible spectrum to which the photographic material in use is nearly, or completely insensitive. Design A safelight usually consists of an ordinary light bulb in a housing closed off by a coloured filter, but sometimes a special light bulb or fluorescent tube with suitable filter material or phosphor (in fluorescent tubes) coated directly on the glass is used in an ordinary fixture. Differently sensitised materials require different safelights. In traditional black-and-white photographic printing, photographic papers normally are handled under an amber or red safelight, as such papers typically are sensitive only to blue and green light. Orthochromatic papers and films are also sensitive to yellow light and must be used only with a deep red safelight, not with an amber one. Panchromatic films and papers, nominally sensitive to the entire spectrum, sometimes ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Chemistry
Chemistry is the science, scientific study of the properties and behavior of matter. It is a natural science that covers the Chemical element, elements that make up matter to the chemical compound, compounds made of atoms, molecules and ions: their composition, structure, properties, behavior and the changes they undergo during a Chemical reaction, reaction with other Chemical substance, substances. Chemistry also addresses the nature of chemical bonds in chemical compounds. In the scope of its subject, chemistry occupies an intermediate position between physics and biology. It is sometimes called the central science because it provides a foundation for understanding both Basic research, basic and Applied science, applied scientific disciplines at a fundamental level. For example, chemistry explains aspects of plant growth (botany), the formation of igneous rocks (geology), how atmospheric ozone is formed and how environmental pollutants are degraded (ecology), the properties ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Staining
Staining is a technique used to enhance contrast in samples, generally at the microscopic level. Stains and dyes are frequently used in histology (microscopic study of biological tissues), in cytology (microscopic study of cells), and in the medical fields of histopathology, hematology, and cytopathology that focus on the study and diagnoses of diseases at the microscopic level. Stains may be used to define biological tissues (highlighting, for example, muscle fibers or connective tissue), cell populations (classifying different blood cells), or organelles within individual cells. In biochemistry, it involves adding a class-specific ( DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewin ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
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