6803
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6803
''Synechocystis'' sp. PCC6803 is a strain of unicellular, freshwater cyanobacteria. ''Synechocystis'' sp. PCC6803 is capable of both phototrophic growth by oxygenic photosynthesis during light periods and heterotrophic growth by glycolysis and oxidative phosphorylation during dark periods. Gene expression is regulated by a circadian clock and the organism can effectively anticipate transitions between the light and dark phases. Evolutionary history Cyanobacteria are photosynthetic prokaryotes that have existed on Earth for an estimated 2.7 billion years. The ability of cyanobacteria to produce oxygen initiated the transition from a planet consisting of high levels of carbon dioxide and little oxygen, to what has been called the Great Oxygenation Event where large amounts of oxygen gas were produced. Cyanobacteria have colonized a wide diversity of habitats, including fresh and salt water ecosystems, and most land environments. Phylogenetically, ''Synechocystis'' branches off l ...
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Cyanobacteria
Cyanobacteria (), also known as Cyanophyta, are a phylum of gram-negative bacteria that obtain energy via photosynthesis. The name ''cyanobacteria'' refers to their color (), which similarly forms the basis of cyanobacteria's common name, blue-green algae, although they are not usually scientifically classified as algae. They appear to have originated in a freshwater or terrestrial environment. Sericytochromatia, the proposed name of the paraphyletic and most basal group, is the ancestor of both the non-photosynthetic group Melainabacteria and the photosynthetic cyanobacteria, also called Oxyphotobacteria. Cyanobacteria use photosynthetic pigments, such as carotenoids, phycobilins, and various forms of chlorophyll, which absorb energy from light. Unlike heterotrophic prokaryotes, cyanobacteria have internal membranes. These are flattened sacs called thylakoids where photosynthesis is performed. Phototrophic eukaryotes such as green plants perform photosynthesis in plast ...
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Cyanobacteria
Cyanobacteria (), also known as Cyanophyta, are a phylum of gram-negative bacteria that obtain energy via photosynthesis. The name ''cyanobacteria'' refers to their color (), which similarly forms the basis of cyanobacteria's common name, blue-green algae, although they are not usually scientifically classified as algae. They appear to have originated in a freshwater or terrestrial environment. Sericytochromatia, the proposed name of the paraphyletic and most basal group, is the ancestor of both the non-photosynthetic group Melainabacteria and the photosynthetic cyanobacteria, also called Oxyphotobacteria. Cyanobacteria use photosynthetic pigments, such as carotenoids, phycobilins, and various forms of chlorophyll, which absorb energy from light. Unlike heterotrophic prokaryotes, cyanobacteria have internal membranes. These are flattened sacs called thylakoids where photosynthesis is performed. Phototrophic eukaryotes such as green plants perform photosynthesis in plast ...
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Cyanophyceae
Cyanobacteria (), also known as Cyanophyta, are a phylum of gram-negative bacteria that obtain energy via photosynthesis. The name ''cyanobacteria'' refers to their color (), which similarly forms the basis of cyanobacteria's common name, blue-green algae, although they are not usually scientifically classified as algae. They appear to have originated in a freshwater or terrestrial environment. Sericytochromatia, the proposed name of the paraphyletic and most basal group, is the ancestor of both the non-photosynthetic group Melainabacteria and the photosynthetic cyanobacteria, also called Oxyphotobacteria. Cyanobacteria use photosynthetic pigments, such as carotenoids, phycobilins, and various forms of chlorophyll, which absorb energy from light. Unlike heterotrophic prokaryotes, cyanobacteria have internal membranes. These are flattened sacs called thylakoids where photosynthesis is performed. Phototrophic eukaryotes such as green plants perform photosynthesis in plastids t ...
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Phototaxis
Phototaxis is a kind of taxis, or locomotory movement, that occurs when a whole organism moves towards or away from a stimulus of light. This is advantageous for phototrophic organisms as they can orient themselves most efficiently to receive light for photosynthesis. Phototaxis is called positive if the movement is in the direction of increasing light intensity and negative if the direction is opposite. Two types of positive phototaxis are observed in prokaryotes. The first is called scotophobotaxis (from the word " scotophobia"), which is observed only under a microscope. This occurs when a bacterium swims by chance out of the area illuminated by the microscope. Entering darkness signals the cell to reverse flagella rotation direction and reenter the light. The second type of phototaxis is true phototaxis, which is a directed movement up a gradient to an increasing amount of light. This is analogous to positive chemotaxis except that the attractant is light rather than a chem ...
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Synechocystis
''Synechocystis'' is a genus of unicellular, freshwater cyanobacteria in the family Merismopediaceae. It includes a strain, ''Synechocystis'' sp. PCC 6803, which is a well studied model organism. Like all cyanobacteria, ''Synechocystis'' branches on the evolutionary tree from its ancestral root, ''Gloeobacter violaceus''. ''Synechocystis'' is not diazotrophic, and is closely related to another model organism, ''Cyanothece ''Cyanothece'' is a genus of unicellular, diazotrophic, oxygenic photosynthesizing cyanobacteria. Modern organisms and cellular organization In 1976, Jiří Komárek defined the prokaryotic cyanobacteria genus ''Cyanothece'' as distinct from ' ...'' ATCC 51442. It has been suggested that originally ''Synechocystis'' possessed the ability to fix atmospheric nitrogen, but lost the genes required for the process. See also * ''Synechocystis'' run-and-tumble References {{Taxonbar, from=Q7662346 Cyanobacteria genera Synechococcales Bacteria genera< ...
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Electroporation
Electroporation, or electropermeabilization, is a microbiology technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane, allowing chemicals, drugs, electrode arrays or DNA to be introduced into the cell (also called electrotransfer). In microbiology, the process of electroporation is often used to transform bacteria, yeast, or plant protoplasts by introducing new coding DNA. If bacteria and plasmids are mixed together, the plasmids can be transferred into the bacteria after electroporation, though depending on what is being transferred, cell-penetrating peptides or CellSqueeze could also be used. Electroporation works by passing thousands of volts (~8 kV/cm) across suspended cells in an electroporation cuvette. Afterwards, the cells have to be handled carefully until they have had a chance to divide, producing new cells that contain reproduced plasmids. This process is approximately ten times more effective in increasing ...
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Celsius
The degree Celsius is the unit of temperature on the Celsius scale (originally known as the centigrade scale outside Sweden), one of two temperature scales used in the International System of Units (SI), the other being the Kelvin scale. The degree Celsius (symbol: °C) can refer to a specific temperature on the Celsius scale or a unit to indicate a difference or range between two temperatures. It is named after the Swedish astronomer Anders Celsius (1701–1744), who developed a similar temperature scale in 1742. Before being renamed in 1948 to honour Anders Celsius, the unit was called ''centigrade'', from the Latin ''centum'', which means 100, and ''gradus'', which means steps. Most major countries use this scale; the other major scale, Fahrenheit, is still used in the United States, some island territories, and Liberia. The Kelvin scale is of use in the sciences, with representing absolute zero. Since 1743 the Celsius scale has been based on 0 °C for the freezing ...
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Covalent Bond
A covalent bond is a chemical bond that involves the sharing of electrons to form electron pairs between atoms. These electron pairs are known as shared pairs or bonding pairs. The stable balance of attractive and repulsive forces between atoms, when they share electrons, is known as covalent bonding. For many molecules, the sharing of electrons allows each atom to attain the equivalent of a full valence shell, corresponding to a stable electronic configuration. In organic chemistry, covalent bonding is much more common than ionic bonding. Covalent bonding also includes many kinds of interactions, including σ-bonding, π-bonding, metal-to-metal bonding, agostic interactions, bent bonds, three-center two-electron bonds and three-center four-electron bonds. The term ''covalent bond'' dates from 1939. The prefix ''co-'' means ''jointly, associated in action, partnered to a lesser degree, '' etc.; thus a "co-valent bond", in essence, means that the atoms share " valence", such a ...
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Diazotroph
Diazotrophs are bacteria and archaea that fix gaseous nitrogen in the atmosphere into a more usable form such as ammonia. A diazotroph is a microorganism that is able to grow without external sources of fixed nitrogen. Examples of organisms that do this are rhizobia and ''Frankia'' (in symbiosis) and ''Azospirillum''. All diazotrophs contain iron-molybdenum or iron-vanadium nitrogenase systems. Two of the most studied systems are those of ''Klebsiella pneumoniae'' and ''Azotobacter vinelandii''. These systems are studied because of their genetic tractability and their fast growth. Etymology The word diazotroph is derived from the words ''diazo'' ("di" = two + "azo" = nitrogen) meaning "dinitrogen (N2)" and ''troph'' meaning "pertaining to food or nourishment", in summary dinitrogen utilizing. The word ''azote'' means nitrogen in French and was named by French chemist and biologist Antoine Lavoisier, who saw it as the part of air which cannot sustain life. Types of diazotrophs Diaz ...
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Freshwater
Fresh water or freshwater is any naturally occurring liquid or frozen water containing low concentrations of dissolved salts and other total dissolved solids. Although the term specifically excludes seawater and brackish water, it does include non- salty mineral-rich waters such as chalybeate springs. Fresh water may encompass frozen and meltwater in ice sheets, ice caps, glaciers, snowfields and icebergs, natural precipitations such as rainfall, snowfall, hail/ sleet and graupel, and surface runoffs that form inland bodies of water such as wetlands, ponds, lakes, rivers, streams, as well as groundwater contained in aquifers, subterranean rivers and lakes. Fresh water is the water resource that is of the most and immediate use to humans. Water is critical to the survival of all living organisms. Many organisms can thrive on salt water, but the great majority of higher plants and most insects, amphibians, reptiles, mammals and birds need fresh water to survive. Fresh wa ...
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Environmental Stress
Stress, either physiological, biological or psychological, is an organism's response to a stressor such as an environmental condition. Stress is the body's method of reacting to a condition such as a threat, challenge or physical and psychological barrier. There are two hormones that an individual produces during a stressful situation, these are well known as adrenaline and cortisol. There are two kinds of stress hormone levels. Resting (basal) cortisol levels are normal everyday quantities that are essential for standard functioning. Reactive cortisol levels are increases in cortisol in response to stressors. Stimuli that alter an organism's environment are responded to by multiple systems in the body. In humans and most mammals, the autonomic nervous system and hypothalamic-pituitary-adrenal (HPA) axis are the two major systems that respond to stress. The sympathoadrenal medullary (SAM) axis may activate the fight-or-flight response through the sympathetic nervous sys ...
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Plastids
The plastid (Greek: πλαστός; plastós: formed, molded – plural plastids) is a membrane-bound organelle found in the cells of plants, algae, and some other eukaryotic organisms. They are considered to be intracellular endosymbiotic cyanobacteria. Examples include chloroplasts (used for photosynthesis), chromoplasts (used for pigment synthesis and storage), and leucoplasts (non-pigmented plastids that can sometimes differentiate). The event which led to permanent endosymbiosis in the Archaeplastida clade (of land plants, red algae, and green algae) probably occurred with a cyanobiont (a symbiotic cyanobacteria) related to the genus '' Gloeomargarita'', around 1.5 billion years ago. A later primary endosymbiosis event occurred in photosynthetic ''Paulinella'' amoeboids about 90–140 million years ago. This plastid belongs to the "PS-clade" (of the cyanobacteria genera ''Prochlorococcus'' and ''Synechococcus''). Secondary and tertiary endosymbiosis has also occurred, in a wi ...
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