Mabinlin
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Mabinlin
Mabinlins are sweet, sweet-tasting proteins extracted from the seed of mabinlang (''Capparis masaikai Levl.''), a plant growing in Yunnan province of China. There are four homologues. Mabinlin-2 was first isolated in 1983 and characterised in 1993, and is the most extensively studied of the four. The other variants of mabinlin-1, -3 and -4 were discovered and characterised in 1994. Protein structures The 4 mabinlins are very similar in their Amino acid#Table of standard amino acid abbreviations and properties, amino acids peptide sequence, sequences (see below). ''Chain A'' M-1: M-2: M-3: M-4: ''Chain B'' M-1: M-2: M-3: M-4: ''Amino acid sequence of Mabinlins homologues are adapted from Swiss-Prot biological database of protein. The molecular weights of Mabinlin-1, Mabinlin-3 and Mabinlin-4 are 12.3 Atomic mass unit, kDa, 12.3 kDa and 11.9 kDa, respectively. With a molecular weight of 10.4kDa, mabinlin-2 is lighter than mabinlin-1. It is a heterodimer consisting ...
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Mabinlang
''Capparis masaikai'', known as mabinlang,Data Portal of the Global Biodiversity Information Facility (GBIF)Biodiversity occurrence data for Capparis masaikai H. Léveillé grows in the subtropical region of the Yunnan province of China and bear fruits of tennis-ball size. The mature seeds are used in traditional Chinese medicine. They are also used as sweets; the seeds elicit a sweet taste when chewed.X Liu, S Maeda, Z Hu, T Aiuchi, K Nakaya, Y Kurihara. Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II. Eur J Biochem 1993. 211(1–2):281-7. The origin of the sweet taste was identified as sweet-tasting proteins named mabinlin Mabinlins are sweet-tasting proteins extracted from the seed of mabinlang ('' Capparis masaikai Levl.''), a plant growing in Yunnan province of China. There are four homologues. Mabinlin-2 was first isolated in 1983 and characterised in 1993, ...s. They are highly sweet, 100-400 ...
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Capparis Masaikai
''Capparis masaikai'', known as mabinlang,Data Portal of the Global Biodiversity Information Facility (GBIF)Biodiversity occurrence data for Capparis masaikai H. Léveillé grows in the subtropical region of the Yunnan province of China and bear fruits of tennis-ball size. The mature seeds are used in traditional Chinese medicine. They are also used as sweets; the seeds elicit a sweet taste when chewed.X Liu, S Maeda, Z Hu, T Aiuchi, K Nakaya, Y Kurihara. Purification, complete amino acid sequence and structural characterization of the heat-stable sweet protein, mabinlin II. Eur J Biochem 1993. 211(1–2):281-7. The origin of the sweet taste was identified as sweet-tasting proteins named mabinlin Mabinlins are sweet, sweet-tasting proteins extracted from the seed of mabinlang (''Capparis masaikai Levl.''), a plant growing in Yunnan province of China. There are four homologues. Mabinlin-2 was first isolated in 1983 and characterised in ...s. They are highly sweet, 100-400 ...
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Pentadin
Pentadin, a sweet-tasting protein, was discovered and isolated in 1989, in the fruit of Oubli (''Pentadiplandra brazzeana'' ), a climbing shrub growing in some tropical countries of Africa. The fruit has been consumed by the apes and the natives for a long time. The berries of the plant were incredibly sweet African locals call them "j'oublie" (French for "I forget") because their taste helps nursing infants forget their mothers' milk. Pentadin, with brazzein discovered in 1994, are the 2 sweet-tasting proteins discovered in this African fruit. Pentadin molecular weight estimated to be 12kDa. It is reported to be 500 times sweeter than sucrose Sucrose, a disaccharide, is a sugar composed of glucose and fructose subunits. It is produced naturally in plants and is the main constituent of white sugar. It has the molecular formula . For human consumption, sucrose is extracted and refined ... on a weight basis, with its sweetness having a slow onset and decline similar to monelli ...
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Monellin
Monellin, a sweet protein, was discovered in 1969 in the fruit of the West African shrub known as serendipity berry (''Dioscoreophyllum cumminsii''); it was first reported as a carbohydrate.GE Inglett, JF May. Serendipity berries - Source of a new intense sweetener. J Food Sci 1969, 34:408-411. The protein was named in 1972 after the Monell Chemical Senses Center in Philadelphia, U.S.A., where it was isolated and characterized. Protein composition Monellin's molecular weight is 10.7 Atomic mass unit, kDa. It has two noncovalently associated polypeptide chains: an A chain sequence with 44 amino acid residues, and a B chain with 50 residues. Monellin chain A (44 AA): Monellin chain B (50 AA): Amino acid#Table of standard amino acid abbreviations and properties, Amino acid sequence of the sweet protein monellin adapted from Swiss-Prot biological database of protein. Monellin has a secondary structure consisting of five beta-strands that form an antiparallel beta-sheet and a ...
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Sweet
Sweetness is a basic taste most commonly perceived when eating foods rich in sugars. Sweet tastes are generally regarded as pleasurable. In addition to sugars like sucrose, many other chemical compounds are sweet, including aldehydes, ketones, and sugar alcohols. Some are sweet at very low concentrations, allowing their use as non-caloric sugar substitutes. Such non-sugar sweeteners include saccharin and aspartame. Other compounds, such as miraculin, may alter perception of sweetness itself. The perceived intensity of sugars and high-potency sweeteners, such as Aspartame and Neohesperidin Dihydrochalcone, are heritable, with gene effect accounting for approximately 30% of the variation. The chemosensory basis for detecting sweetness, which varies between both individuals and species, has only begun to be understood since the late 20th century. One theoretical model of sweetness is the multipoint attachment theory, which involves multiple binding sites between a sweetnes ...
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Sugar Substitute
A sugar substitute is a food additive that provides a sweetness like that of sugar while containing significantly less food energy than sugar-based sweeteners, making it a zero-calorie () or low-calorie sweetener. Artificial sweeteners may be derived through manufacturing of plant extracts or processed by chemical synthesis. Sugar substitute products are commercially available in various forms, such as small pills, powders, and packets. In North America, common sugar substitutes include aspartame, monk fruit extract, saccharin, sucralose, and stevia; cyclamate is also used outside the United States. These sweeteners are a fundamental ingredient in diet drinks to sweeten them without adding calories. Additionally, sugar alcohols such as erythritol, xylitol, and sorbitol are derived from sugars. Approved artificial sweeteners do not cause cancer. Reviews and dietetic professionals have concluded that moderate use of non-nutritive sweeteners as a safe replacement for sugars can he ...
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Thaumatin
Thaumatin (also known as talin) is a low-calorie sweetener and flavor modifier. The protein is often used primarily for its flavor-modifying properties and not exclusively as a sweetener. The thaumatins were first found as a mixture of proteins isolated from the katemfe fruit (''Thaumatococcus daniellii'') (Marantaceae) of West Africa. Although very sweet, thaumatin's taste is markedly different from sugar's. The sweetness of thaumatin builds very slowly. Perception lasts a long time, leaving a liquorice-like aftertaste at high concentrations. Thaumatin is highly water soluble, stable to heating, and stable under acidic conditions. Biological role Thaumatin production is induced in katemfe in response to an attack upon the plant by viroid pathogens. Several members of the thaumatin protein family display significant ''in vitro'' inhibition of hyphal growth and sporulation by various fungi. The thaumatin protein is considered a prototype for a pathogen-response protein do ...
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Brazzein
Brazzein is a sweet-tasting protein extracted from the West African fruit of the climbing plant Oubli (''Pentadiplandra brazzeana'' Henri Ernest Baillon, Baillon). It was first isolated by the University of Wisconsin–Madison in 1994. Brazzein is found in the extracellular region, in the pulp tissue surrounding the seeds. With pentadin, discovered in 1989, brazzein is the second sweet-tasting protein discovered in the Oubli fruit. Like the other sweet proteins discovered in plants, such as monellin and thaumatin, it is extremely sweet compared to commonly used sweeteners (500 to 2000 times sweeter than sucrose). The fruit tastes sweet to humans, monkeys, and bonobos, but gorillas have mutations in their sweetness receptors so that they do not find brazzein sweet, and they are not known to eat the fruit. Traditional use The Oubli plant (from which the protein was isolated) grows in Gabon and Cameroon, where its fruit has been consumed by the apes and local people for a long time ...
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University Of Hawaii
A university () is an institution of higher (or tertiary) education and research which awards academic degrees in several academic disciplines. Universities typically offer both undergraduate and postgraduate programs. In the United States, the designation is reserved for colleges that have a graduate school. The word ''university'' is derived from the Latin ''universitas magistrorum et scholarium'', which roughly means "community of teachers and scholars". The first universities were created in Europe by Catholic Church monks. The University of Bologna (''Università di Bologna''), founded in 1088, is the first university in the sense of: *Being a high degree-awarding institute. *Having independence from the ecclesiastic schools, although conducted by both clergy and non-clergy. *Using the word ''universitas'' (which was coined at its foundation). *Issuing secular and non-secular degrees: grammar, rhetoric, logic, theology, canon law, notarial law.Hunt Janin: "The university ...
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Potato
The potato is a starchy food, a tuber of the plant ''Solanum tuberosum'' and is a root vegetable native to the Americas. The plant is a perennial in the nightshade family Solanaceae. Wild potato species can be found from the southern United States to southern Chile. The potato was originally believed to have been domesticated by Native Americans independently in multiple locations,University of Wisconsin-Madison, ''Finding rewrites the evolutionary history of the origin of potatoes'' (2005/ref> but later genetic studies traced a single origin, in the area of present-day southern Peru and extreme northwestern Bolivia. Potatoes were domesticated there approximately 7,000–10,000 years ago, from a species in the ''Solanum brevicaule'' complex. Lay summary: In the Andes region of South America, where the species is indigenous, some close relatives of the potato are cultivated. Potatoes were introduced to Europe from the Americas by the Spanish in the second half of the 16 ...
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Genetically Modified Organism
A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques. The exact definition of a genetically modified organism and what constitutes genetic engineering varies, with the most common being an organism altered in a way that "does not occur naturally by mating and/or natural recombination". A wide variety of organisms have been genetically modified (GM), from animals to plants and microorganisms. Genes have been transferred within the same species, across species (creating transgenic organisms), and even across kingdoms. New genes can be introduced, or endogenous genes can be enhanced, altered, or knocked out. Creating a genetically modified organism is a multi-step process. Genetic engineers must isolate the gene they wish to insert into the host organism and combine it with other genetic elements, including a promoter and terminator region and often a selectable marker. A number of techniques are a ...
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Sucrose
Sucrose, a disaccharide, is a sugar composed of glucose and fructose subunits. It is produced naturally in plants and is the main constituent of white sugar. It has the molecular formula . For human consumption, sucrose is extracted and refined from either sugarcane or sugar beet. Sugar mills – typically located in tropical regions near where sugarcane is grown – crush the cane and produce raw sugar which is shipped to other factories for refining into pure sucrose. Sugar beet factories are located in temperate climates where the beet is grown, and process the beets directly into refined sugar. The sugar-refining process involves washing the raw sugar crystals before dissolving them into a sugar syrup which is filtered and then passed over carbon to remove any residual colour. The sugar syrup is then concentrated by boiling under a vacuum and crystallized as the final purification process to produce crystals of pure sucrose that are clear, odorless, and sweet. Suga ...
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