Hill Reagent
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Hill Reagent
Discovered in 1937 by Robin Hill (biochemist), Robin Hill, Hill reagents allowed the discovery of electron transport chains during photosynthesis. These are dyes that act as artificial electron acceptors, changing color when they are reduced. An example of a Hill reagent is 2,6-dichlorophenolindophenol (DCPIP). References

* *{{cite journal , title = Discoveries in Oxygenic Photosynthesis (1727–2003): A Perspective , author = Govindjee and David Krogmann , journal = Photosynthesis Research , volume = 80 , issue = 1–3 , pages = 15–57 , year = 2004 , url = , doi = 10.1023/B:PRES.0000030443.63979.e6 , pmid = 16328809 Oxidizing agents Photosynthesis ...
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Robin Hill (biochemist)
Robert Hill Royal Society, FRS (2 April 1899 – 15 March 1991), known as Robin Hill, was a British biochemistry, plant biochemist who, in 1939, demonstrated the 'Hill reaction' of photosynthesis, proving that oxygen is Oxygen evolution, evolved during the light-dependent reaction, light requiring steps of photosynthesis. He also made significant contributions to the development of the Z-scheme of oxygenic photosynthesis. Education and early life Hill was born in New Milverton, a suburb of Leamington Spa, Warwickshire. He was educated at Bedales School, where he became interested in biology and astronomy (he published a paper on sunspots in 1917), and Emmanuel College, Cambridge, where he read Natural Sciences (Cambridge), Natural Sciences. During the First World War he served in the Anti-gas Department of the Royal Engineers. Career In 1922, he joined the Department of Biochemistry at Cambridge, where he was directed to research hemoglobin. He published a number of papers on h ...
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Photosynthesis
Photosynthesis is a process used by plants and other organisms to convert light energy into chemical energy that, through cellular respiration, can later be released to fuel the organism's activities. Some of this chemical energy is stored in carbohydrate molecules, such as sugars and starches, which are synthesized from carbon dioxide and water – hence the name ''photosynthesis'', from the Greek ''phōs'' (), "light", and ''synthesis'' (), "putting together". Most plants, algae, and cyanobacteria perform photosynthesis; such organisms are called photoautotrophs. Photosynthesis is largely responsible for producing and maintaining the oxygen content of the Earth's atmosphere, and supplies most of the energy necessary for life on Earth. Although photosynthesis is performed differently by different species, the process always begins when energy from light is absorbed by proteins called reaction centers that contain green chlorophyll (and other colored) pigments/chromoph ...
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DCPIP
2,6-Dichlorophenolindophenol (DCPIP, DCIP or DPIP) is a chemical compound used as a redox dye. When oxidized, DCPIP is blue with a maximal absorption at 600 nm; when reduced, DCPIP is colorless. DCPIP can be used to measure the rate of photosynthesis. It is part of the Hill reagents family. When exposed to light in a photosynthetic system, the dye is decolorised by chemical reduction. DCPIP has a higher affinity for electrons than ferredoxin and the photosynthetic electron transport chain can reduce DCPIP as a substitute for NADP+, that is normally the final electron carrier in photosynthesis. As DCPIP is reduced and becomes colorless, the resultant increase in light transmittance can be measured using a spectrophotometer. DCPIP can also be used as an indicator for vitamin C. If vitamin C, which is a good reducing agent, is present, the blue dye, which turns pink in acid conditions, is reduced to a colorless compound by ascorbic acid. This reaction is a redox reac ...
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Oxidizing Agents
An oxidizing agent (also known as an oxidant, oxidizer, electron recipient, or electron acceptor) is a substance in a redox chemical reaction that gains or " accepts"/"receives" an electron from a (called the , , or ). In other words, an oxidizer is any substance that oxidizes another substance. The oxidation state, which describes the degree of loss of electrons, of the oxidizer decreases while that of the reductant increases; this is expressed by saying that oxidizers "undergo reduction" and "are reduced" while reducers "undergo oxidation" and "are oxidized". Common oxidizing agents are oxygen, hydrogen peroxide and the halogens. In one sense, an oxidizing agent is a chemical species that undergoes a chemical reaction in which it gains one or more electrons. In that sense, it is one component in an oxidation–reduction (redox) reaction. In the second sense, an oxidizing agent is a chemical species that transfers electronegative atoms, usually oxygen, to a substrate. Combust ...
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