Herbaspirillum Lusitanum
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Herbaspirillum Lusitanum
''Herbaspirillum lusitanum'' is a nitrogen-fixing bacterium found in root nodules of common beans (''Phaseolus vulgaris''). Phylogenetic In biology, phylogenetics (; from Greek φυλή/ φῦλον [] "tribe, clan, race", and wikt:γενετικός, γενετικός [] "origin, source, birth") is the study of the evolutionary history and relationships among or within groups o ... analyses have shown this bacterium belongs to the genus ''Herbaspirillum''. ''H. lusitanum'' lacks the ''nif'' gene. A ''nodD''-like gene is present, but no other ''nod'' genes have been identified. The lack of ''nif'' and ''nod'' genes suggests ''H. lusitanum'' is an opportunistic bacterium capable of colonizing root nodules, but is unable to fix nitrogen. References External linksType strain of ''Herbaspirillum lusitanum'' at Bac''Dive'' - the Bacterial Diversity Metadatabase Burkholderiales Bacteria described in 2003 {{Betaproteobacteria-stub ...
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Nitrogen-fixing
Nitrogen fixation is a chemical process by which molecular nitrogen (), with a strong triple covalent bond, in the air is converted into ammonia () or related nitrogenous compounds, typically in soil or aquatic systems but also in industry. Atmospheric nitrogen is molecular dinitrogen, a relatively nonreactive molecule that is metabolically useless to all but a few microorganisms. Biological nitrogen fixation or ''diazotrophy'' is an important microbials mediated process that converts dinitrogen (N2) gas to ammonia (NH3) using the nitrogenase protein complex (Nif). Nitrogen fixation is essential to life because fixed inorganic nitrogen compounds are required for the biosynthesis of all nitrogen-containing organic compounds, such as amino acids and proteins, nucleoside triphosphates and nucleic acids. As part of the nitrogen cycle, it is essential for agriculture and the manufacture of fertilizer. It is also, indirectly, relevant to the manufacture of all nitrogen chemical compo ...
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Bacterium
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationshi ...
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Root Nodules
Root nodules are found on the roots of plants, primarily legumes, that form a symbiosis with nitrogen-fixing bacteria. Under nitrogen-limiting conditions, capable plants form a symbiotic relationship with a host-specific strain of bacteria known as rhizobia. This process has evolved multiple times within the legumes, as well as in other species found within the Rosid clade. Legume crops include beans, peas, and soybeans. Within legume root nodules, nitrogen gas (N2) from the atmosphere is converted into ammonia (NH3), which is then assimilated into amino acids (the building blocks of proteins), nucleotides (the building blocks of DNA and RNA as well as the important energy molecule ATP), and other cellular constituents such as vitamins, flavones, and hormones. Their ability to fix gaseous nitrogen makes legumes an ideal agricultural organism as their requirement for nitrogen fertilizer is reduced. Indeed, high nitrogen content blocks nodule development as there is no benefit fo ...
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Phaseolus Vulgaris
''Phaseolus vulgaris'', the common bean, is a herbaceous annual plant grown worldwide for its edible dry seeds or green, unripe pods. Its leaf is also occasionally used as a vegetable and the straw as fodder. Its botanical classification, along with other '' Phaseolus'' species, is as a member of the legume family Fabaceae. Like most members of this family, common beans acquire the nitrogen they require through an association with rhizobia, which are nitrogen-fixing bacteria. The common bean has a long history of cultivation. All wild members of the species have a climbing habit, but many cultivars are classified either as ''bush beans'' or ''climbing beans'', depending on their style of growth. Best-known cultivar groups include the kidney bean, the navy bean, the pinto bean, and the wax bean. The other major types of commercially grown beans are the runner bean (''Phaseolus coccineus'') and the broad bean (''Vicia faba''). Beans are grown on every continent except Antarcti ...
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Phylogenetic
In biology, phylogenetics (; from Greek φυλή/ φῦλον [] "tribe, clan, race", and wikt:γενετικός, γενετικός [] "origin, source, birth") is the study of the evolutionary history and relationships among or within groups of organisms. These relationships are determined by Computational phylogenetics, phylogenetic inference methods that focus on observed heritable traits, such as DNA sequences, protein amino acid sequences, or morphology. The result of such an analysis is a phylogenetic tree—a diagram containing a hypothesis of relationships that reflects the evolutionary history of a group of organisms. The tips of a phylogenetic tree can be living taxa or fossils, and represent the "end" or the present time in an evolutionary lineage. A phylogenetic diagram can be rooted or unrooted. A rooted tree diagram indicates the hypothetical common ancestor of the tree. An unrooted tree diagram (a network) makes no assumption about the ancestral line, and does ...
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Nif Gene
The ''nif'' genes are genes encoding enzymes involved in the fixation of atmospheric nitrogen into a form of nitrogen available to living organisms. The primary enzyme encoded by the ''nif'' genes is the nitrogenase complex which is in charge of converting atmospheric nitrogen (N2) to other nitrogen forms such as ammonia which the organism can use for various purposes. Besides the nitrogenase enzyme, the ''nif'' genes also encode a number of regulatory proteins involved in nitrogen fixation. The ''nif'' genes are found in both free-living nitrogen-fixing bacteria and in symbiotic bacteria associated with various plants. The expression of the ''nif'' genes is induced as a response to low concentrations of fixed nitrogen and oxygen concentrations (the low oxygen concentrations are actively maintained in the root environment of host plants). The first Rhizobium genes for nitrogen fixation (nif) and for nodulation (nod) were cloned in the early 1980s by Gary Ruvkun and Sharon R. Long i ...
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Root Nodule
Root nodules are found on the roots of plants, primarily legumes, that form a symbiosis with nitrogen-fixing bacteria. Under nitrogen-limiting conditions, capable plants form a symbiotic relationship with a host-specific strain of bacteria known as rhizobia. This process has evolved multiple times within the legumes, as well as in other species found within the Rosid clade. Legume crops include beans, peas, and soybeans. Within legume root nodules, nitrogen gas (N2) from the atmosphere is converted into ammonia (NH3), which is then assimilated into amino acids (the building blocks of proteins), nucleotides (the building blocks of DNA and RNA as well as the important energy molecule ATP), and other cellular constituents such as vitamins, flavones, and hormones. Their ability to fix gaseous nitrogen makes legumes an ideal agricultural organism as their requirement for nitrogen fertilizer is reduced. Indeed, high nitrogen content blocks nodule development as there is no benefit ...
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Burkholderiales
The Burkholderiales are an order of Pseudomonadota.George M. Garrity: '' Bergey's Manual of Systematic Bacteriology''. 2. Auflage. Springer, New York, 2005, Vol. 2: ''The Proteobacteria Part C: The Alpha-, Beta-, Delta-, and Epsilonproteabacteria'' Like all Pseudomonadota, they are Gram-negative. They include several pathogenic bacteria, including species of ''Burkholderia'', ''Bordetella'', and ''Ralstonia''. They also include '' Oxalobacter'' and related genera, which are unusual in using oxalic acid as their source of carbon. Other well-studied genera include ''Alcaligenes'', '' Cupriavidus'', ''Achromobacter'', ''Comamonas'', '' Delftia'', ''Massilia'', '' Duganella'', '' Janthinobacterium'', ''Polynucleobacter'' (important freshwater bacterioplankton), non-pathogenic ''Paraburkholderia'', '' Caballeronia'', '' Polaromonas'', ''Thiomonas'', ''Collimonas'', '' Hydrogenophaga'', ''Sphaerotilus'', '' Variovorax'', '' Acidovorax'', '' Rubrivivax'' and ''Rhodoferax'' (both members ...
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