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GUS Assay
The GUS reporter system (''GUS'': β-glucuronidase) is a reporter gene system, particularly useful in plant molecular biology and microbiology. Several kinds of GUS reporter gene assay are available, depending on the substrate used. The term GUS staining refers to the most common of these, a histochemical technique. Purpose The purpose of this technique is to analyze the activity of a gene transcription promoter (in terms of expression of a so-called reporter gene under the regulatory control of that promoter) either in a quantitative manner, involving some measure of activity, or qualitatively (on versus off) through visualization of its activity in different cells, tissues, or organs. The technique utilizes the uidA gene of Escherichia coli, which codes for the enzyme, β-glucuronidase; this enzyme, when incubated with specific colorless or non-fluorescent substrates, can convert them into stable colored or fluorescent products. The presence of the GUS-induced color indicates ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
5-bromo-4-chloro-3-indolyl Glucuronide
X-Gluc is a chemical compound with the molecular formula C14H13BrClNO7. It is used as a reagent to detect β-glucuronidase, an enzyme produced by the '' E. coli'' bacterium. It is used to detect ''E. coli'' contamination in food, water and the urinary tract. In addition, it is widely used in molecular biology experiments to mark and select the expression of target genes (GUS reporter system). It is often preferred to other detection methods because it is fast (24-hour testing), relatively accurate ( <1% false negatives and <5% false positives ) and the result is easy to observe and interpret. The reagent is safe to transport and easy to store. See also * *X-gal
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Algae
Algae (; singular alga ) is an informal term for a large and diverse group of photosynthetic eukaryotic organisms. It is a polyphyletic grouping that includes species from multiple distinct clades. Included organisms range from unicellular microalgae, such as ''Chlorella,'' ''Prototheca'' and the diatoms, to multicellular forms, such as the giant kelp, a large brown alga which may grow up to in length. Most are aquatic and autotrophic (they generate food internally) and lack many of the distinct cell and tissue types, such as stomata, xylem and phloem that are found in land plants. The largest and most complex marine algae are called seaweeds, while the most complex freshwater forms are the ''Charophyta'', a division of green algae which includes, for example, ''Spirogyra'' and stoneworts. No definition of algae is generally accepted. One definition is that algae "have chlorophyll ''a'' as their primary photosynthetic pigment and lack a sterile covering of cells around thei ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Mosses
Mosses are small, non-vascular flowerless plants in the taxonomic division Bryophyta (, ) ''sensu stricto''. Bryophyta (''sensu lato'', Schimp. 1879) may also refer to the parent group bryophytes, which comprise liverworts, mosses, and hornworts. Mosses typically form dense green clumps or mats, often in damp or shady locations. The individual plants are usually composed of simple leaves that are generally only one cell thick, attached to a stem that may be branched or unbranched and has only a limited role in conducting water and nutrients. Although some species have conducting tissues, these are generally poorly developed and structurally different from similar tissue found in vascular plants. Mosses do not have seeds and after fertilisation develop sporophytes with unbranched stalks topped with single capsules containing spores. They are typically tall, though some species are much larger. ''Dawsonia'', the tallest moss in the world, can grow to in height. There are app ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Spermatophyte
A spermatophyte (; ), also known as phanerogam (taxon Phanerogamae) or phaenogam (taxon Phaenogamae), is any plant that produces seeds, hence the alternative name seed plant. Spermatophytes are a subset of the embryophytes or land plants. They include most familiar types of plants, including all flowers and most trees, but exclude some other types of plants such as ferns, mosses, algae. The term ''phanerogams'' or ''phanerogamae'' is derived from the Greek (), meaning "visible", in contrast to the cryptogamae (), together with the suffix (), meaning "to marry". These terms distinguished those plants with hidden sexual organs (cryptogamae) from those with visible sexual organs (phanerogamae). Description The extant spermatophytes form five divisions, the first four of which are traditionally grouped as gymnosperms, plants that have unenclosed, "naked seeds": * Cycadophyta, the cycads, a subtropical and tropical group of plants, * Ginkgophyta, which includes a single living s ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Molluscs
Mollusca is the second-largest phylum of invertebrate animals after the Arthropoda, the members of which are known as molluscs or mollusks (). Around 85,000 extant taxon, extant species of molluscs are recognized. The number of fossil species is estimated between 60,000 and 100,000 additional species. The proportion of undescribed species is very high. Many taxa remain poorly studied. Molluscs are the largest marine biology, marine phylum, comprising about 23% of all the named marine organisms. Numerous molluscs also live in freshwater mollusc, freshwater and Terrestrial molluscs, terrestrial habitats. They are highly diverse, not just in size and anatomical structure, but also in behaviour and habitat. The phylum is typically divided into 7 or 8 Taxonomy (biology), taxonomic class (biology), classes, of which two are entirely extinct. Cephalopod molluscs, such as squid, cuttlefish, and octopuses, are among the most neurobiology, neurologically advanced of all inve ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Vertebrates
Vertebrates () comprise all animal taxa within the subphylum Vertebrata () ( chordates with backbones), including all mammals, birds, reptiles, amphibians, and fish. Vertebrates represent the overwhelming majority of the phylum Chordata, with currently about 69,963 species described. Vertebrates comprise such groups as the following: * jawless fish, which include hagfish and lampreys * jawed vertebrates, which include: ** cartilaginous fish (sharks, rays, and ratfish) ** bony vertebrates, which include: *** ray-fins (the majority of living bony fish) *** lobe-fins, which include: **** coelacanths and lungfish **** tetrapods (limbed vertebrates) Extant vertebrates range in size from the frog species ''Paedophryne amauensis'', at as little as , to the blue whale, at up to . Vertebrates make up less than five percent of all described animal species; the rest are invertebrates, which lack vertebral columns. The vertebrates traditionally include the hagfish, which do no ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Rice Embryo
Rice is the seed of the grass species ''Oryza sativa'' (Asian rice) or less commonly ''Oryza glaberrima'' (African rice). The name wild rice is usually used for species of the genera ''Zizania'' and ''Porteresia'', both wild and domesticated, although the term may also be used for primitive or uncultivated varieties of ''Oryza''. As a cereal grain, domesticated rice is the most widely consumed staple food for over half of the world's human population,Abstract, "Rice feeds more than half the world's population." especially in Asia and Africa. It is the agricultural commodity with the third-highest worldwide production, after sugarcane and maize. Since sizable portions of sugarcane and maize crops are used for purposes other than human consumption, rice is the most important food crop with regard to human nutrition and caloric intake, providing more than one-fifth of the calories consumed worldwide by humans. There are many varieties of rice and culinary preferences tend to vary ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Cambridge
Cambridge ( ) is a university city and the county town in Cambridgeshire, England. It is located on the River Cam approximately north of London. As of the 2021 United Kingdom census, the population of Cambridge was 145,700. Cambridge became an important trading centre during the Roman and Viking ages, and there is archaeological evidence of settlement in the area as early as the Bronze Age. The first town charters were granted in the 12th century, although modern city status was not officially conferred until 1951. The city is most famous as the home of the University of Cambridge, which was founded in 1209 and consistently ranks among the best universities in the world. The buildings of the university include King's College Chapel, Cavendish Laboratory, and the Cambridge University Library, one of the largest legal deposit libraries in the world. The city's skyline is dominated by several college buildings, along with the spire of the Our Lady and the English Martyrs ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
University Of Colorado At Boulder
The University of Colorado Boulder (CU Boulder, CU, or Colorado) is a public research university in Boulder, Colorado. Founded in 1876, five months before Colorado became a state, it is the flagship university of the University of Colorado system. CU Boulder is a member of the Association of American Universities, a selective group of major research universities in North America, and is classified among R1: Doctoral Universities – Very high research activity. In 2021, the university attracted support of over $634 million for research and spent $536 million on research and development according to the National Science Foundation, ranking it 50th in the nation. The university consists of nine colleges and schools and offers over 150 academic programs, enrolling more than 35,000 students as of January 2022. To date, 5 Nobel Prize laureates, 10 Pulitzer Prize winners, 11 MacArthur "Genius Grant" recipients, 1 Turing Award laureate, and 20 astronauts have been affiliated with ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Richard Anthony Jefferson
Richard Anthony Jefferson (born 1956) is an American-born molecular biologist and social entrepreneur who developed the widely used reporter gene system GUS, conducted the world's first biotech crop release, proposed the Hologenome theory of evolution, pioneered Biological Open Source and founded The Lens. He is founder of the social enterprise Cambia and a professor of Biological Innovation at the Queensland University of Technology. In 2003 he was named by Scientific American as one of the world's 50 most influential technologists, and is renowned for his work on making science-enabled innovation more widely accessible. He was profiled in 'Open & Shut: The Basement Interviews', and other major media, including in an Economist Feature 'Grassroots Innovator' in 2001. Education Born in Santa Cruz, California, Jefferson studied at the University of California, Santa Barbara at the College of Creative Studies, and obtained his BA (Molecular Genetics) in 1978. He then moved t ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
Beta-galactosidase
β-Galactosidase (EC 3.2.1.23, lactase, beta-gal or β-gal; systematic name β-D-galactoside galactohydrolase), is a glycoside hydrolase enzyme that catalyzes hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides. β-Galactosides include carbohydrates containing galactose where the glycosidic bond lies above the galactose molecule. Substrates of different β-galactosidases include ganglioside GM1, lactosylceramides, lactose, and various glycoproteins. Function β-Galactosidase is an exoglycosidase which hydrolyzes the β-glycosidic bond formed between a galactose and its organic moiety. It may also cleave fucosides and arabinosides but with much lower efficiency. It is an essential enzyme in the human body. Deficiencies in the protein can result in galactosialidosis or Morquio B syndrome. In '' E. coli'', the ''lacZ'' gene is the structural gene for β-galactosidase; which is present as part of the inducible system ''lac'' operon which is activ ... [...More Info...] [...Related Items...] OR: [Wikipedia] [Google] [Baidu] |
