X-Gluc is a chemical compound with the molecular formula C₁₄H₁₃BrClNO₇. It is used as a

reagent In chemistry, a reagent ( ) or analytical reagent is a substance or compound added to a system to cause a chemical reaction, or test if one occurs. The terms ''reactant'' and ''reagent'' are often used interchangeably, but reactant specifies a ...

to detect β-glucuronidase, an enzyme produced by the ''

E. coli ''Escherichia coli'' (),Wells, J. C. (2000) Longman Pronunciation Dictionary. Harlow ngland Pearson Education Ltd. also known as ''E. coli'' (), is a Gram-negative, facultative anaerobic, rod-shaped, coliform bacterium of the genus ''Escher ...

'' bacterium. It is used to detect ''E. coli'' contamination in food, water and the urinary tract. In addition, it is widely used in molecular biology experiments to mark and select the expression of target genes ( GUS reporter system). It is often preferred to other detection methods because it is fast (24-hour testing), relatively accurate ( <1% false negatives and <5% false positives ) and the result is easy to observe and interpret. The reagent is safe to transport and easy to store.

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X-Gluc lab exercise
Susan J Karcher, Purdue University
Histochemical localization of GUS reporter activity in plant tissues
Stanislav Vitha, Texas A&M University Indoles Chloroarenes Glucuronide esters Bromoarenes Reagents

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References

External links