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Flg-Rhizobiales RNA Motif
The ''flg''-Rhizobiales RNA motif is an RNA structure that is conserved in certain bacteria. All known ''flg''-Rhizobiales RNAs are located in the presumptive 5' untranslated regions of operons that contain genes whose functions relate to the creation of flagellar basal bodies. The ''flg''-Rhizobiales RNAs are restricted to the Hyphomicrobiales (formerly known as Rhizobiales), an order of alphaproteobacteria Alphaproteobacteria is a class of bacteria in the phylum Pseudomonadota (formerly Proteobacteria). The Magnetococcales and Mariprofundales are considered basal or sister to the Alphaproteobacteria. The Alphaproteobacteria are highly diverse and p ..., although only some Rhizobiales bacterial are predicted to use ''flg''-Rhizobiales RNAs. The exact function of these RNAs is unknown, although it is hypothesized that they have a ''cis''-regulatory function in controlling expression of the downstream operons. See also * Rhizobiales-2 RNA motif References External links ...
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Hyphomicrobiales
The ''Hyphomicrobiales'' are an order of Gram-negative Alphaproteobacteria. The rhizobia, which fix nitrogen and are symbiotic with plant roots, appear in several different families. The four families ''Nitrobacteraceae'', ''Hyphomicrobiaceae'', '' Phyllobacteriaceae'', and ''Rhizobiaceae'' contain at least several genera of nitrogen-fixing, legume-nodulating, microsymbiotic bacteria. Examples are the genera ''Bradyrhizobium'' and ''Rhizobium''. Species of the ''Methylocystaceae'' are methanotrophs; they use methanol (CH3OH) or methane (CH4) as their sole energy and carbon sources. Other important genera are the human pathogens ''Bartonella'' and ''Brucella'', as well as ''Agrobacterium'' (useful in genetic engineering). Taxonomy Accepted families * ''Aestuariivirgaceae'' Li ''et al''. 2019 * '' Afifellaceae'' Hördt ''et al''. 2020 * '' Ahrensiaceae'' Hördt ''et al''. 2020 * ''Alsobacteraceae'' Sun ''et al''. 2018 * ''Amorphaceae'' Hördt ''et al''. 2020 * ''Ancalomicrobia ...
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Bacteria
Bacteria (; singular: bacterium) are ubiquitous, mostly free-living organisms often consisting of one biological cell. They constitute a large domain of prokaryotic microorganisms. Typically a few micrometres in length, bacteria were among the first life forms to appear on Earth, and are present in most of its habitats. Bacteria inhabit soil, water, acidic hot springs, radioactive waste, and the deep biosphere of Earth's crust. Bacteria are vital in many stages of the nutrient cycle by recycling nutrients such as the fixation of nitrogen from the atmosphere. The nutrient cycle includes the decomposition of dead bodies; bacteria are responsible for the putrefaction stage in this process. In the biological communities surrounding hydrothermal vents and cold seeps, extremophile bacteria provide the nutrients needed to sustain life by converting dissolved compounds, such as hydrogen sulphide and methane, to energy. Bacteria also live in symbiotic and parasitic relationsh ...
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5' Untranslated Region
The 5′ untranslated region (also known as 5′ UTR, leader sequence, transcript leader, or leader RNA) is the region of a messenger RNA (mRNA) that is directly Upstream and downstream (DNA), upstream from the initiation codon. This region is important for the regulation of translation (biology), translation of a transcript by differing mechanisms in viruses, prokaryotes and eukaryotes. While called untranslated, the 5′ UTR or a portion of it is sometimes translated into a protein product. This product can then regulate the translation of the main coding sequence of the mRNA. In many organisms, however, the 5′ UTR is completely untranslated, instead forming a complex Nucleic acid secondary structure, secondary structure to regulate translation. The 5′ UTR has been found to interact with proteins relating to metabolism, and within the 5′ UTR. In addition, this region has been involved in Transcription (biology), transcription regulation, such as the Drosophila melanogaste ...
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Operon
In genetics, an operon is a functioning unit of DNA containing a cluster of genes under the control of a single promoter. The genes are transcribed together into an mRNA strand and either translated together in the cytoplasm, or undergo splicing to create monocistronic mRNAs that are translated separately, i.e. several strands of mRNA that each encode a single gene product. The result of this is that the genes contained in the operon are either expressed together or not at all. Several genes must be ''co-transcribed'' to define an operon. Originally, operons were thought to exist solely in prokaryotes (which includes organelles like plastids that are derived from bacteria), but since the discovery of the first operons in eukaryotes in the early 1990s, more evidence has arisen to suggest they are more common than previously assumed. In general, expression of prokaryotic operons leads to the generation of polycistronic mRNAs, while eukaryotic operons lead to monocistronic mRNA ...
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Gene
In biology, the word gene (from , ; "...Wilhelm Johannsen coined the word gene to describe the Mendelian units of heredity..." meaning ''generation'' or ''birth'' or ''gender'') can have several different meanings. The Mendelian gene is a basic unit of heredity and the molecular gene is a sequence of nucleotides in DNA that is transcribed to produce a functional RNA. There are two types of molecular genes: protein-coding genes and noncoding genes. During gene expression, the DNA is first copied into RNA. The RNA can be directly functional or be the intermediate template for a protein that performs a function. The transmission of genes to an organism's offspring is the basis of the inheritance of phenotypic traits. These genes make up different DNA sequences called genotypes. Genotypes along with environmental and developmental factors determine what the phenotypes will be. Most biological traits are under the influence of polygenes (many different genes) as well as gen ...
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Flagella
A flagellum (; ) is a hairlike appendage that protrudes from certain plant and animal sperm cells, and from a wide range of microorganisms to provide motility. Many protists with flagella are termed as flagellates. A microorganism may have from one to many flagella. A gram-negative bacterium ''Helicobacter pylori'' for example uses its multiple flagella to propel itself through the mucus lining to reach the stomach epithelium, where it may cause a gastric ulcer to develop. In some bacteria the flagellum can also function as a sensory organelle, being sensitive to wetness outside the cell. Across the three domains of Bacteria, Archaea, and Eukaryota the flagellum has a different structure, protein composition, and mechanism of propulsion but shares the same function of providing motility. The Latin word means " whip" to describe its lash-like swimming motion. The flagellum in archaea is called the archaellum to note its difference from the bacterial flagellum. Eukaryoti ...
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Order (biology)
Order ( la, wikt:ordo#Latin, ordo) is one of the eight major hierarchical taxonomic ranks in Linnaean taxonomy. It is classified between Family_(biology), family and Class_(biology), class. In biological classification, the order is a taxonomic rank used in the classification of organisms and recognized by the nomenclature codes. An immediately higher rank, superorder, is sometimes added directly above order, with suborder directly beneath order. An order can also be defined as a group of related families. What does and does not belong to each order is determined by a taxonomist, as is whether a particular order should be recognized at all. Often there is no exact agreement, with different taxonomists each taking a different position. There are no hard rules that a taxonomist needs to follow in describing or recognizing an order. Some taxa are accepted almost universally, while others are recognized only rarely. The name of an order is usually written with a capital letter. Fo ...
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Alphaproteobacteria
Alphaproteobacteria is a class of bacteria in the phylum Pseudomonadota (formerly Proteobacteria). The Magnetococcales and Mariprofundales are considered basal or sister to the Alphaproteobacteria. The Alphaproteobacteria are highly diverse and possess few commonalities, but nevertheless share a common ancestor. Like all ''Proteobacteria'', its members are gram-negative and some of its intracellular parasitic members lack peptidoglycan and are consequently gram variable. Characteristics The Alphaproteobacteria are a diverse taxon and comprises several phototrophic genera, several genera metabolising C1-compounds (''e.g.'', ''Methylobacterium'' spp.), symbionts of plants (''e.g.'', ''Rhizobium'' spp.), endosymbionts of arthropods (''Wolbachia'') and intracellular pathogens (''e.g. Rickettsia''). Moreover, the class is sister to the protomitochondrion, the bacterium that was engulfed by the eukaryotic ancestor and gave rise to the mitochondria, which are organelles in eukaryotic ce ...
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Cis-regulatory Element
''Cis''-regulatory elements (CREs) or ''Cis''-regulatory modules (CRMs) are regions of non-coding DNA which regulate the transcription of neighboring genes. CREs are vital components of genetic regulatory networks, which in turn control morphogenesis, the development of anatomy, and other aspects of embryonic development, studied in evolutionary developmental biology. CREs are found in the vicinity of the genes that they regulate. CREs typically regulate gene transcription by binding to transcription factors. A single transcription factor may bind to many CREs, and hence control the expression of many genes ( pleiotropy). The Latin prefix ''cis'' means "on this side", i.e. on the same molecule of DNA as the gene(s) to be transcribed. CRMs are stretches of DNA, usually 100–1000 DNA base pairs in length, where a number of transcription factors can bind and regulate expression of nearby genes and regulate their transcription rates. They are labeled as ''cis'' because they are ...
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Rhizobiales-2 RNA Motif
The Rhizobiales-2 RNA motif is a set of RNAs found in certain bacteria that are presumed to be homologous because they conserve a common primary and secondary structure (see diagram). The motif was discovered using bioinformatics, and is found only within bacteria that belong to the order Hyphomicrobiales (formerly Rhizobiales), in turn a kind of alphaproteobacteria. Because Rhizobiales-2 RNAs are not consistently located in proximity to genes of a consistent class or function, these RNAs are presumed to function as non-coding RNAs. See also *Flg-Rhizobiales RNA motif The ''flg''-Rhizobiales RNA motif is an RNA structure that is conserved in certain bacteria. All known ''flg''-Rhizobiales RNAs are located in the presumptive 5' untranslated regions of operons that contain genes whose functions relate to the c ... References External links * Non-coding RNA {{molecular-cell-biology-stub ...
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