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Durham Tube
Durham tubes are used in microbiology to detect production of gas by microorganisms. They are simply smaller test tubes inserted upside down in another test tube so they are freely movable. The culture media to be tested is then added to the larger tube and sterilized, which also eliminates the initial air gap produced when the tube is inserted upside down. The culture media typically contains a single substance to be tested with the organism, such as to determine whether an organism can ferment a particular carbohydrate. After inoculation and incubation, any gas that is produced will form a visible gas bubble inside the small tube. Litmus Litmus is a water-soluble mixture of different dyes extracted from lichens. It is often absorbed onto filter paper to produce one of the oldest forms of pH indicator, used to test materials for acidity. It is a purple dye that is extracted fro ... solution can also be added to the culture media to give a visual representation of pH ...
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Test Tube
A test tube, also known as a culture tube or sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. Test tubes are usually placed in special-purpose test tube rack, racks. Types and usage Chemistry Test tubes intended for general chemical work are usually made of glass, for its relative resistance to heat. Tubes made from expansion-resistant glasses, mostly borosilicate glass or fused quartz, can withstand high temperatures up to several hundred degrees Celsius. Chemistry tubes are available in a multitude of lengths and widths, typically from 10 to 20 mm wide and 50 to 200 mm long. The top often features a flared lip to aid pouring out the contents. A chemistry test tube typically has a flat bottom, a round bottom, or a conical bottom. Some test tubes are made to accept a ground glass joint, ground glass stopper or a screw cap. They are often provided w ...
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Durham Tube Diagram
Durham most commonly refers to: * Durham, England, a cathedral city and the county town of County Durham * County Durham, an English county *Durham County, North Carolina, a county in North Carolina, United States * Durham, North Carolina, a city in North Carolina, United States Durham may also refer to: Places Australia *Durham, Queensland, an outback locality in the Bulloo Shire of Queensland *Durham Ox, Victoria * Durham Lead, Victoria, a locality in the City of Ballarat Canada *Durham, Nova Scotia *Durham, Ontario, a small town in Grey County, Ontario *Durham County, Ontario, a historic county *Regional Municipality of Durham, a regional government in the Greater Toronto Area of Ontario **Durham (electoral district), a federal electoral district in Durham Region **Durham (provincial electoral district), a provincial electoral district in Durham Region *Durham Bridge, New Brunswick *Durham Parish, New Brunswick * Durham-Sud, Quebec (also known as South Durham) United Kingdom * ...
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Microbiology
Microbiology () is the scientific study of microorganisms, those being unicellular (single cell), multicellular (cell colony), or acellular (lacking cells). Microbiology encompasses numerous sub-disciplines including virology, bacteriology, protistology, mycology, immunology, and parasitology. Eukaryotic microorganisms possess membrane-bound organelles and include fungi and protists, whereas prokaryotic organisms—all of which are microorganisms—are conventionally classified as lacking membrane-bound organelles and include Bacteria and Archaea. Microbiologists traditionally relied on culture, staining, and microscopy. However, less than 1% of the microorganisms present in common environments can be cultured in isolation using current means. Microbiologists often rely on molecular biology tools such as DNA sequence based identification, for example the 16S rRNA gene sequence used for bacteria identification. Viruses have been variably classified as organisms, as they have ...
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Microorganism
A microorganism, or microbe,, ''mikros'', "small") and ''organism'' from the el, ὀργανισμός, ''organismós'', "organism"). It is usually written as a single word but is sometimes hyphenated (''micro-organism''), especially in older texts. The informal synonym ''microbe'' () comes from μικρός, mikrós, "small" and βίος, bíos, "life". is an organism of microscopic size, which may exist in its single-celled form or as a colony of cells. The possible existence of unseen microbial life was suspected from ancient times, such as in Jain scriptures from sixth century BC India. The scientific study of microorganisms began with their observation under the microscope in the 1670s by Anton van Leeuwenhoek. In the 1850s, Louis Pasteur found that microorganisms caused food spoilage, debunking the theory of spontaneous generation. In the 1880s, Robert Koch discovered that microorganisms caused the diseases tuberculosis, cholera, diphtheria, and anthrax. Because mi ...
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Test Tube
A test tube, also known as a culture tube or sample tube, is a common piece of laboratory glassware consisting of a finger-like length of glass or clear plastic tubing, open at the top and closed at the bottom. Test tubes are usually placed in special-purpose test tube rack, racks. Types and usage Chemistry Test tubes intended for general chemical work are usually made of glass, for its relative resistance to heat. Tubes made from expansion-resistant glasses, mostly borosilicate glass or fused quartz, can withstand high temperatures up to several hundred degrees Celsius. Chemistry tubes are available in a multitude of lengths and widths, typically from 10 to 20 mm wide and 50 to 200 mm long. The top often features a flared lip to aid pouring out the contents. A chemistry test tube typically has a flat bottom, a round bottom, or a conical bottom. Some test tubes are made to accept a ground glass joint, ground glass stopper or a screw cap. They are often provided w ...
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Sterilization (microbiology)
Sterilization refers to any process that removes, kills, or deactivates all forms of life (particularly microorganisms such as fungi, bacteria, spores, and unicellular eukaryotic organisms) and other biological agents such as prions present in or on a specific surface, object, or fluid. Sterilization can be achieved through various means, including heat, chemicals, irradiation, high pressure, and filtration. Sterilization is distinct from disinfection, sanitization, and pasteurization, in that those methods reduce rather than eliminate all forms of life and biological agents present. After sterilization, an object is referred to as being sterile or aseptic. Applications Foods One of the first steps toward modernized sterilization was made by Nicolas Appert, who discovered that application of heat over a suitable period slowed the decay of foods and various liquids, preserving them for safe consumption for a longer time than was typical. Canning of foods is an extension of the ...
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Litmus
Litmus is a water-soluble mixture of different dyes extracted from lichens. It is often absorbed onto filter paper to produce one of the oldest forms of pH indicator, used to test materials for acidity. It is a purple dye that is extracted from a type of algal bloom called ‘lichens’. In an acidic medium, blue litmus paper turns red, and red litmus paper turns blue in a basic or alkaline medium. History The word "litmus" comes from an Old Norse word for "pulp". About 1300 the Spanish physician Arnaldus de Villa Nova began using litmus to study acids and bases. From the 16th century onwards, the blue dye was extracted from some lichens, especially in the Netherlands. Natural sources Litmus can be found in different species of lichens. The dyes are extracted from such species as ''Roccella tinctoria'' (South American), ''Roccella fuciformis'' (Angola and Madagascar), ''Roccella pygmaea'' (Algeria), ''Roccella phycopsis'', '' Lecanora tartarea'' (Norway, Sweden), ''Var ...
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Herbert Durham
Herbert Durham DSc (Cantab), MB, BC, FRCS, ARPS (30 March 1866 – 25 October 1945) was a British physician and distinguished scientist. Early life Herbert Edward Durham was born 30 March 1866, the son of Arthur E. Durham, Senior Surgeon to Guy's Hospital. He married Maud Lowry, daughter of Capt. Harmer of 81st Regiment. Education Durham was educated at University College School, London from 1883–84; King's College, Cambridge from 1884; and Guy's Hospital, London. In 1904 he was awarded John Lucas Walker Studentship in Pathology a scholarship given by the University of Cambridge for original pathological research. Career Bacteriology He was Assistant Demonstrator in Histology from 1884–89, then House Surgeon at Guy's Hospital, London from 1889-95. From 1895-96 he worked at the Hygiene Institute, Vienna where he was associated with Professor Max von Gruber in the discovery of agglutination of bacteria. In 1897 he developed an agglutination reaction for diagnosis of typho ...
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