Direct Linear Plot
In biochemistry, the direct linear plot is a graphical method for enzyme kinetics data following the Michaelis–Menten equation. In this plot, observations are not plotted as points, but as ''lines'' in parameter space with axes K_\mathrm and V, such that each observation of a rate v_i at substrate concentration a_i is represented by a straight line with intercept -a_i on the K_\mathrm axis and v_i on the V axis. Ideally (in the absence of experimental error) the lines intersect at a unique point (\hat_\mathrm, \hat) whose coordinates provide the values of \hat_\mathrm and \hat. Comparison with other plots of the Michaelis–Menten equation The best known plots of the Michaelis–Menten equation, including the double-reciprocal plot of 1/v against 1/a, the Hanes plot of a/v against a, and the Eadie–Hofstee plot of v against v/a are all plots in observation space, with each observation represented by a point, and the parameters determined from the slope and intercepts of the lin ...
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