Defocus
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Defocus
In optics, defocus is the aberration in which an image is simply out of focus. This aberration is familiar to anyone who has used a camera, videocamera, microscope, telescope, or binoculars. Optically, defocus refers to a translation of the focus along the optical axis away from the detection surface. In general, defocus reduces the sharpness and contrast of the image. What should be sharp, high-contrast edges in a scene become gradual transitions. Fine detail in the scene is blurred or even becomes invisible. Nearly all image-forming optical devices incorporate some form of focus adjustment to minimize defocus and maximize image quality. In optics and photography The degree of image blurring for a given amount of focus shift depends inversely on the lens f-number. Low f-numbers, such as to 2.8, are very sensitive to defocus and have very shallow depths of focus. High f-numbers, in the 16 to 32 range, are highly tolerant of defocus, and consequently have large depth ...
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Bokeh Example
In photography, bokeh ( or ; ) is the aesthetic quality of the blur produced in out-of-focus parts of an image. Bokeh has also been defined as "the way the lens renders out-of-focus points of light". Differences in lens aberrations and aperture shape cause very different bokeh effects. Some lens designs blur the image in a way that is pleasing to the eye, while others produce distracting or unpleasant blurring ("good" and "bad" bokeh, respectively). Photographers may deliberately use a shallow focus technique to create images with prominent out-of-focus regions, accentuating their lens's bokeh. Bokeh is often most visible around small background highlights, such as specular reflections and light sources, which is why it is often associated with such areas. However, bokeh is not limited to highlights; blur occurs in all regions of an image which are outside the depth of field. The opposite of bokeh—an image in which multiple distances are visible and all are in focu ...
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Aberration In Optical Systems
In optics, aberration is a property of optical systems, such as lenses, that causes light to be spread out over some region of space rather than focused to a point. Aberrations cause the image formed by a lens to be blurred or distorted, with the nature of the distortion depending on the type of aberration. Aberration can be defined as a departure of the performance of an optical system from the predictions of paraxial optics. In an imaging system, it occurs when light from one point of an object does not converge into (or does not diverge from) a single point after transmission through the system. Aberrations occur because the simple paraxial theory is not a completely accurate model of the effect of an optical system on light, rather than due to flaws in the optical elements. An image-forming optical system with aberration will produce an image which is not sharp. Makers of optical instruments need to correct optical systems to compensate for aberration. Aberration can be anal ...
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Zernike Polynomial
In mathematics, the Zernike polynomials are a sequence of polynomials that are orthogonal on the unit disk. Named after optical physicist Frits Zernike, winner of the 1953 Nobel Prize in Physics and the inventor of phase-contrast microscopy, they play important roles in various optics branches such as beam optics and imaging. Definitions There are even and odd Zernike polynomials. The even Zernike polynomials are defined as :Z^_n(\rho,\varphi) = R^m_n(\rho)\,\cos(m\,\varphi) \! (even function over the azimuthal angle \varphi), and the odd Zernike polynomials are defined as :Z^_n(\rho,\varphi) = R^m_n(\rho)\,\sin(m\,\varphi), \! (odd function over the azimuthal angle \varphi) where ''m'' and ''n'' are nonnegative integers with ''n ≥ m ≥ 0'' (''m'' = 0 for even Zernike polynomials), ''\varphi'' is the azimuthal angle, ''ρ'' is the radial distance 0\le\rho\le 1, and R^m_n are the radial polynomials defined below. Zernike polynomials have the property of being l ...
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Focus (optics)
In geometrical optics, a focus, also called an image point, is a point where light rays originating from a point on the object converge. Although the focus is conceptually a point, physically the focus has a spatial extent, called the blur circle. This non-ideal focusing may be caused by aberrations of the imaging optics. In the absence of significant aberrations, the smallest possible blur circle is the Airy disc, which is caused by diffraction from the optical system's aperture. Aberrations tend to worsen as the aperture diameter increases, while the Airy circle is smallest for large apertures. An image, or image point or region, is in focus if light from object points is converged almost as much as possible in the image, and out of focus if light is not well converged. The border between these is sometimes defined using a "circle of confusion" criterion. A principal focus or focal point is a special focus: * For a lens, or a spherical or parabolic mirror, it is a point ...
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Optical Resolution
Optical resolution describes the ability of an imaging system to resolve detail, in the object that is being imaged. An imaging system may have many individual components, including one or more lenses, and/or recording and display components. Each of these contributes (given suitable design, and adequate alignment) to the optical resolution of the system; the environment in which the imaging is done often is a further important factor. Lateral resolution Resolution depends on the distance between two distinguishable radiating points. The sections below describe the theoretical estimates of resolution, but the real values may differ. The results below are based on mathematical models of Airy discs, which assumes an adequate level of contrast. In low-contrast systems, the resolution may be much lower than predicted by the theory outlined below. Real optical systems are complex, and practical difficulties often increase the distance between distinguishable point sources. The ...
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Gerchberg–Saxton Algorithm
The Gerchberg–Saxton (GS) algorithm is an iterative phase retrieval algorithm for retrieving the phase of a complex-valued wavefront from two intensity measurements acquired in two different planes. Typically, the two planes are the image plane and the far field (diffraction) plane, and the wavefront propagation between these two planes is given by the Fourier transform. The original paper by Gerchberg and Saxton considered image and diffraction pattern of a sample acquired in an electron microscope. It is often necessary to know only the phase distribution from one of the planes, since the phase distribution on the other plane can be obtained by performing a Fourier transform on the plane whose phase is known. Although often used for two-dimensional signals, the GS algorithm is also valid for one-dimensional signals. The pseudocode below performs the GS algorithm to obtain a phase distribution for the plane "Source", such that its Fourier transform would have the amplitude distr ...
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Phase Retrieval
Phase retrieval is the process of algorithmically finding solutions to the phase problem. Given a complex signal F(k), of amplitude , F (k), , and phase \psi(k): ::F(k) = , F(k), e^ =\int_^ f(x)\ e^\,dx where ''x'' is an ''M''-dimensional spatial coordinate and ''k'' is an ''M''-dimensional spatial frequency coordinate. Phase retrieval consists of finding the phase that satisfies a set of constraints for a measured amplitude. Important applications of phase retrieval include X-ray crystallography, transmission electron microscopy and coherent diffractive imaging, for which M = 2. Uniqueness theorems for both 1-D and 2-D cases of the phase retrieval problem, including the phaseless 1-D inverse scattering problem, were proven by Klibanov and his collaborators (see References). Problem formulation Here we consider 1-D discrete Fourier transform (DFT) phase retrieval problem. The DFT of a complex signal f /math> is given by F \sum_^ f e^=, F \cdot e^ \quad k=0,1, \ldots, N-1, ...
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Electron Microscopy
An electron microscope is a microscope that uses a beam of accelerated electrons as a source of illumination. As the wavelength of an electron can be up to 100,000 times shorter than that of visible light photons, electron microscopes have a higher resolving power than light microscopes and can reveal the structure of smaller objects. A scanning transmission electron microscope has achieved better than 50  pm resolution in annular dark-field imaging mode and magnifications of up to about 10,000,000× whereas most light microscopes are limited by diffraction to about 200  nm resolution and useful magnifications below 2000×. Electron microscopes use shaped magnetic fields to form electron optical lens systems that are analogous to the glass lenses of an optical light microscope. Electron microscopes are used to investigate the ultrastructure of a wide range of biological and inorganic specimens including microorganisms, cells, large molecules, biopsy samples, ...
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Phase Contrast
Phase-contrast imaging is a method of imaging that has a range of different applications. It exploits differences in the refractive index of different materials to differentiate between structures under analysis. In conventional light microscopy, phase contrast can be employed to distinguish between structures of similar transparency, and to examine crystals on the basis of their double refraction. This has uses in biological, medical and geological science. In X-ray tomography, the same physical principles can be used to increase image contrast by highlighting small details of differing refractive index within structures that are otherwise uniform. In transmission electron microscopy (TEM), phase contrast enables very high resolution (HR) imaging, making it possible to distinguish features a few Angstrom apart (at this point highest resolution is 40 pm). Light microscopy Phase contrast takes advantage of the fact that different structures have different refractive indices, and ei ...
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Radius Of Curvature (optics)
Radius of curvature (ROC) has specific meaning and sign convention in optical design. A spherical lens or mirror surface has a center of curvature located either along or decentered from the system local optical axis. The vertex of the lens surface is located on the local optical axis. The distance from the vertex to the center of curvature is the radius of curvature of the surface. The sign convention for the optical radius of curvature is as follows: * If the vertex lies to the left of the center of curvature, the radius of curvature is positive. * If the vertex lies to the right of the center of curvature, the radius of curvature is negative. Thus when viewing a biconvex lens from the side, the left surface radius of curvature is positive, and the right radius of curvature is negative. Note however that ''in areas of optics other than design'', other sign conventions are sometimes used. In particular, many undergraduate physics textbooks use the Gaussian sign conventi ...
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Surface Vertex
In Gaussian optics, the cardinal points consist of three pairs of points located on the optical axis of a rotationally symmetric, focal, optical system. These are the '' focal points'', the principal points, and the nodal points. For ''ideal'' systems, the basic imaging properties such as image size, location, and orientation are completely determined by the locations of the cardinal points; in fact only four points are necessary: the focal points and either the principal or nodal points. The only ideal system that has been achieved in practice is the plane mirror, however the cardinal points are widely used to ''approximate'' the behavior of real optical systems. Cardinal points provide a way to analytically simplify a system with many components, allowing the imaging characteristics of the system to be approximately determined with simple calculations. Explanation The cardinal points lie on the optical axis of the optical system. Each point is defined by the effect the opti ...
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Tangent
In geometry, the tangent line (or simply tangent) to a plane curve at a given point is the straight line that "just touches" the curve at that point. Leibniz defined it as the line through a pair of infinitely close points on the curve. More precisely, a straight line is said to be a tangent of a curve at a point if the line passes through the point on the curve and has slope , where ''f'' is the derivative of ''f''. A similar definition applies to space curves and curves in ''n''-dimensional Euclidean space. As it passes through the point where the tangent line and the curve meet, called the point of tangency, the tangent line is "going in the same direction" as the curve, and is thus the best straight-line approximation to the curve at that point. The tangent line to a point on a differentiable curve can also be thought of as a '' tangent line approximation'', the graph of the affine function that best approximates the original function at the given point. Similarly, t ...
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